정상 임신에서 임신주기에 따른 갑상선기능의 변화

김원배,정재훈,윤보현,이석인,김민선,오태근,조보연,이홍규,고창순 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

It is well known that normal pregnancy is accompanied by a rise in serum concentrations of thyroxine-binding globulin(TBG) and human chorionic gonadotropin (hCG). Alterations of biochemical parameters of thyroid function are recognized during gestation and sensitive tests to evaluate the alterations easily are required. Therefore, a cross-sectional study was undertaken in 140 healthy pregnant women to evaluate the efficacy of free T_4 measured by 2-step RIA compared to other thyroid function tests and to confirm the changes of thyroid function according to the stages of normal pregnancy. The sensitivities of free T_4 index, free T_4(by 2-step RIA), T_3 and TSH were realtively high(99.3%, 93.6%, 92.9%, 83.6%, respectively) compared to those of T_4 and T_3 bead upgake(49.3%, 21.4%) during all stages of pregnancy. There were positive correlations between free T_4 index and free T_4 or total T_4(r=0.68, r=0.72; p$lt;0.001). The values of free T_4 index sharply decreased from 3.22+-0.10(meam +-SEM) during 6th-12th week to an plateau after 16th-20th week of gestation(p$lt;0.01). The serum concentrations free T_4 and T_3 bead uptake also significantly decreased from 1.65+-0.05 ng/dl, 24.7+- 0.7% during 6th-12th week to an plateau after 16th-20th week of gestation, respectively(p$lt;0.001), No differences were found in the changes of serum concentrations of T_3, T_4 and TSH according to the stages of pregnancy. In conclusion, it is adequate to measure some tests including free T_4 index and free T_4 to evaluate thyroid function during pregnancy. The thyroid physiology and changes of thyroid function according to the stages of pregnancy should be considered in the interpretation of thyroid function status during pregnancy(J Kor Soc Endocrinol 9: 183-189, 1994).

여호와 증인 환자에서 산부인과적 무혈 수술의 임상적 고찰

이성욱,오일영,서상기,반태학,박윤희,박성철,한광수 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.5

목적 : 여호와의 증인은 자신들의 교리에 따라 혈액이나 기타 혈액제재의 사용을 거부하는 종파이다. 이들에 대한 산부인과적 무혈 수술의 임상상 및 수술후 결과를 후향적으로 검토하였다. 연구방법 : 1995년 2월부터 1999년 8월까지 세종병원에서 입원 기간 중 어떠한 혈액이나 혈액제재를 투여 받지 않았던 여호와의 증인 환자 50예에 대해 무혈 수술의 성적을 후향적으로 조사하였다. 결과 : 환자의 연령 분포는 9세와 58세 사이로 평균 36.7 ± 8.7세 이었다. 무혈 수술의 가장 흔한 사례는 제왕 절개술이었다. 인간 조혈촉진인자(EPO)를 제왕 절개술 5예, 전자궁 적출술 2예, 난소 종양 2예에 투여하였다. EPO를 투여 받은 9예에서는 평균 혈색소 수치가 수술 전에 비해 수술 후 오히려 의미있게 증가하였다. 조직학적 결과로는 자궁 선근증 9예, 평활근종 8예, 생식 세포종 3예, 자궁외 임신 2예, 자궁 상피내 종양 1예, 상피성 난소 종양 1예, 출혈성 황체 낭종 1예, 성기삭 간질성 종양 1예로 총 27예이었다. 무혈 수술로 인한 술 후 사망은 없었으며 이환율도 적었다. 결론 : 여호와의 증인 환자에 대한 산부인과적 수술이 요구될 때 세심한 수술 기법과 다양한 혈액 보존법 등을 이용한다면 비교적 양호한 무혈 수술의 결과를 얻을 수 있었다. 그러나 향후 여호와의 증인 환자에 대한 특수화된 프로그램의 개발이 필요하다고 사료되었다. Objective : Jehovah's Witnesses are members of a religious denomination whose beliefs prohibit the use of blood or blood products. Plasma volume expanders and extracorporeal hemodilution of the patients's own blood are theologically acceptiable. And so we evaluated effectiveness of major operative procedures carried out on Jehovas's Witnesses patients without blood transfusions or blood products. Methods : We retrospectively studied 50 cases of Jehovas's Witnesses who underwent obstetric and gynecologic bloodless surgery from February 1995 to August 1999. No patients recieved any blood or blood products during hospitalizaion. Results : The age distribution of patients was between 9 years and 58 year, average 36.7±8.7 years old. The most common cases for bloodless surgery was Cesarean section. The recombinant human erythropoietin(EPO) was treated in 5 cases of Cesarean section, 2 cases of TAH and 2 cases of ovarian tumor perioperatively. EPO-treated 9 cases were increased postoperative mean Hb levels compared with preoperative mean Hb levels. Histology revealed 9 cases of adenomyosis, 8 cases of leiomyoma, 3 cases of germ cell tumor, 2 cases of ectopic pregnancy, 1 case of CIS, 1 case of Krukenberg tumor, 1 case of epithelial cell tumor, 1 case of hemorrhagic corpus tumor and 1 case of sexcord stromal tumor, total 27 cases. There was no operative death and postoperative morbidities were few. Conclusions : We concluded that if we preformed the surgeries with careful operative techniques and various blood conserving procedures we could do the bloodless surgery of Jehovah's Witnesses with relative safety. But the establishment of a well programmed special medical supports for the Jehovah's Witnesses community will be needed in future.

포장방법과 저장조건이 돈육 등심의 저장성 및 보수력에 미치는 영향

허선진,주선태,오성현,김영직,김영환,이정일,박구부 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.1

To investigate the effects of packaging method and storage condition on meat quality, a total of eight pork loins were selected from a commercial meat plant at 24 hr postmortem. The loins were cut to 2.5㎝ thick steaks and were randomly assigned to the five treatment combination; vacuum packaging and submersion in chilled salt water(SCSW)(T1), non-vacuum packaging and submersion in chilled salt water (T2), vacuum packaging and storage in conventional refrigerator(SCR)(T3), non-vacuum packaging and storage in conventional refrigerator(T4), and wrap packaging and storage in conventional refrigerator(TS). All samples were stored at 0℃ for 20 days to measure color of meat(CIE L*, a*. b*), pH, drip loss, purge loss %, lipid oxidation (TBARS; thiobarbituric acid reactive substances) and total plate counts (TPC). CIE L* and b* value of SCSW(T1, 2) samples were significantly(P$lt;0.05) lower than SCR samples when they were measured at storage 20 days. The sample of SCSW(T1, 2) showed significantly(P$lt;0.05) lower pH than those of SCR(T3, 4, 5) during storage. The sample of SCSW(T1, 2) showed significantly(P$lt;0.05) higher Drip loss than those of SCR(T3, 4, 5) during 5 days storage. T2 showed the lowest purge loss % whereas T3 remarked the highest purge loss %. The samples of SCSW showed significantly(P$lt;0.05) lower TBARS than those of SCR during storage. TPC was samples of SCSW(T1, 2) showed significantly(P$lt;0.05) lower than other treatments. These results suggested that NVP(non-vacuum packaging) and SCSW could reduce purge loss and discoloration, and extend shelf-life of pork loin.

소화 효소 및 시간에 따른 고섬유소 사료의 In vitro 소화율 측정법

오서영(S. Y. Oh),이민영(M. Y. Lee),김재만(J. M. Kim),이지환(J. H. Lee),윤원(W. Yun),송태화(T. H. Song),정연근(Y. K. Cheong),조진호(J. H. Cho) 충북대학교 동물생명과학연구소 2015 동물생명과학연구 Vol.7 No.-

This study was conducted to investigate assay of high fiber diet in vitro digestibility according to digestive enzyme and time. Treatment of this study included: 1) CON (basic method) 2) T1 (sample amount: 2 times; enzyme level: 2 times) 3) T2 (sample amount: 2 times, enzyme level: 2 times, assay time: 1.5 times) 4) T3 (sample amount: 2 times, enzyme level: 2 times, assay time: 2 times). With regard to dry matter (DM), organic matter (OM) digestibility, T3 and CON were higher than T1 and T2 (P<0.05). Also, with regard to DM and OM digestibility by time of digestion, base was similar T2. In conclusion, in vitro digestibility was closely related to digestion time.

Heme oxygenase-1 mediates nicotine- and lipopolysaccharide-induced expression of cyclooxygenase-2 and inducible nitric oxide synthase in human periodontal ligament cells

Pi, S.-H.,Jeong, G.-S.,Oh, H.-W.,Kim, Y.-S.,Pae, H.-O.,Chung, H.-T.,Lee, S.-K.,Kim, E.-C. Blackwell Publishing Ltd 2010 Journal of periodontal research Vol.45 No.2

<P><I>Pi S-H, Jeong G-S, Oh H-W, Kim Y-S, Pae H-O, Chung H-T, Lee S-K, Kim E-C. Heme oxygenase-1 mediates nicotine- and lipopolysaccharide-induced expression of cyclooxygenase-2 and inducible nitric oxide synthase in human periodontal ligament cells. J Periodont Res 2010; 45: 177–183. © 2010 John Wiley & Sons A/S</I></P><P>Background and Objective: </P><P>Although heme oxygenase-1 (HO-1) plays a key role in inflammation, its anti-inflammatory effects and mechanism of action in periodontitis are still unknown. This study aimed to identify the effects of HO-1 on the proinflammatory mediators activated by nicotine and lipopolysaccharide (LPS) stimulation in human periodontal ligament (PDL) cells.</P><P>Material and Methods: </P><P>The production of nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) was evaluated using Griess reagent and an enzyme immunoassay, respectively. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and HO-1 proteins was evaluated by Western blot analysis.</P><P>Results: </P><P>Lipopolysaccharide and nicotine synergistically induced the production of NO and PGE<SUB>2</SUB> and increased the protein expression of iNOS, COX-2 and HO-1. Treatment with an HO-1 inhibitor and HO-1 small interfering RNAs blocked the LPS- and nicotine-stimulated NO and PGE<SUB>2</SUB> release as well as the expression of iNOS and COX-2.</P><P>Conclusion: </P><P>Our data suggest that the nicotine- and LPS-induced inflammatory effects on PDL cells may act through a novel mechanism involving the action of HO-1. Thus, HO-1 may provide a potential therapeutic target for the treatment of periodontal disease associated with smoking and dental plaque.</P>

Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea

Yoon, J.-H.,Kang, S.-J.,Lee, M.-H.,Oh, T.-K. Microbiology Society 2008 International journal of systematic and evolutiona Vol.58 No.8

<P>A Gram-negative, non-motile, rod-shaped, agarolytic bacterial strain, designated JW-26T, was isolated from seawater off Jeju Island in Korea, and was subjected to a polyphasic taxonomic investigation. Strain JW-26T grew optimally at pH 7.0-8.0, at 30 degrees C and in the presence of approximately 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain JW-26T joins Tamlana crocina HST1-43T, with which it exhibited a 16S rRNA gene sequence similarity value of 95.5 %. Strain JW-26T contained MK-6 as the predominant menaquinone and iso-C15:0, C15:0 and iso-C15:1 as the major fatty acids. The DNA G+C content was 36.8 mol%. There were no distinct phenotypic and, particularly, chemotaxonomic properties to differentiate strain JW-26T from the genus Tamlana. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain JW-26T is distinguishable from the type strain of T. crocina. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain JW-26T represents a novel species within the genus Tamlana, for which the name Tamlana agarivorans sp. nov. is proposed. The type strain is JW-26T (=KCTC 22176T =CCUG 55327T).</P>

사료급여방법이 제주재래마의 체성장 및 도체품질에 미치는 영향

오운용(W . Y . Oh),강태홍(T . H . Kang),김동철(D . C . Kim),진신흠(S . H . Jin),홍성귀(S . K . Hong),양승주(S . J . Yang),정재홍(J . H . Jung) 한국축산학회 1993 한국축산학회지 Vol.35 No.6

Nine Cheju Native Horses with an average weight of 169.2㎏ were used in a 238-d (34-wk) fattening trial designed to evaluate the effects of feeding method on growth performance and carcass characteristics. Horses were randomly alloted to three treatment groups: T₁ 6-wk (Former fattening period: FFP) + 28-wk (Latter fattening period: LFP), T₂, 9-wk (FFP) + 25-wk (LFP), T₃, 12-wk (FFP) + 22-wk (LFP). During the FFP, concentrate was restricted at approximately l.5% of their body weights but horses were allowed ad libitum access to concentrate during the subsequent LFP. The horses were individually penned in 2.5-m×3-m pipe stalls with front feeding gates. They were allowed 3 h of exercise in a pen (50-m×30-m) outside the barn monthly. Horses were weighed at 30-d intervals and feed consumption was measured daily. At the end of the 238-d fattening period, one horse in each treatment was selected randomly and slaughtered for meat quality evaluation. Results obtained from the present study were summarized as follows; during the overall fattening period, average daily gains (ADG) and nutrient intake (TDN) per ㎏ wt, gain were 0.59, 5.10(T₁), 0.51, 5.84(T₂) and 0.43, 6.37㎏(T₂), respectively. When horses were allowed ad libitum access to concentrate for 28 wk(T₁), ADG and feed efficiency(TDN) were improved by 37%(P$gt;.05) and20%(P$gt;.05)compared to those allowed concentrate free choice for 22 wk(T₃). Horse in T, group had an improved dressing percentage and carcass weight with good quality grades(intramuscular fat, meat color, fat color). Taste panelists(150 amateures) detected no difference from longissimus lumborum steaks between Cheju Native Horse and improved Cheju Native Cattle, but found juiciness and meat flavor were superior to those of beef.

Association of tumor necrosis factor- gene polymorphisms with advanced stage endometriosis

Lee, G. H.,Choi, Y. M.,Kim, S. H.,Hong, M. A.,Oh, S. T.,Lim, Y. T.,Moon, S. Y. Oxford University Press 2008 Human reproduction Vol.23 No.4

<P>BACKGROUND: This study was performed to investigate whether specific haplotypes and several single nucleotide polymorphisms in the promoter region of the tumor necrosis factor (TNF)-alpha gene are associated with the risk of advanced stage endometriosis in a Korean population. METHODS: This study comprised women with (n = 246) or without (n = 248) endometriosis. The TNF:g.[-1031T > C], TNF:g.[-863C > A] and TNF:g.[-857C > T] polymorphism in the TNF-alpha gene were assessed by PCR-restriction fragment length polymorphism analysis, which utilized digestion by BbsI, HypCH4IV and HypCH4IV restriction enzymes, respectively. In silico haplotypes were deduced by using the Haploview version 3.32. RESULTS: The genotype distribution of TNF:g.[-1031T > C] was significantly different between total endometriosis patients and the controls (T/T of 56.9 versus 60.1%, T/C of 35.4 versus 37.5% and C/C of 7.7 versus 2.4%, respectively, P = 0.027). This difference at the TNF:g.[-1031T > C] tends to increase in Stage IV endometriosis (P = 0.01). However, there was no difference in the TNF:g.[-863C > A] and TNF:g.[-857C > T] site between the two groups. Even when the endometriosis cases were subdivided into American Society for Reproductive Medicine Stages III and IV, genotype differences were not found. The CC homozygote at TNF:g.-863 was more frequently found in the controls than Non-CC group (P = 0.04; odds ratio = 0.67; 95% confidence interval = 0.45-0.98). All haplotypes and diplotypes, deduced by in silico analysis, showed no association with subgroups or controls. CONCLUSIONS: Our results suggest that the genotype frequencies at the TNF:g.[-1031T > C] and the TNF:g.[-863C > A] sites may be associated with advanced stage endometriosis in the Korean population.</P>

Common promoter polymorphism in monocyte differentiation antigen CD14 is associated with serum triglyceride levels and body mass index in non-diabetic individuals

Shin, H. D.,Park, K. S.,Park, B. L.,Cheong, H. S.,Cho, Y. M.,Lee, H. K.,Lee, J.-Y.,Lee, J.-K.,Kim, H. T.,Han, B. G.,Kim, J. W.,Koh, I.,Kim, Y. J.,Oh, B.,Kimm, K.,Park, C. Blackwell Science Ltd 2006 Diabetic medicine Vol.23 No.1

<P>Abstract</P><P>Aims </P><P>Growing evidence supports the hypothesis that chronic low-grade inflammation related to innate immunity may play an important role in the pathophysiology of Type 2 diabetes mellitus (T2DM). The monocyte differentiation antigen CD14 gene (<I>CD14</I>) acts as the receptor for lipopolysaccharide (LPS) and augments monocyte/macrophage inflammatory responses.</P><P>Methods </P><P>We have sequenced the gene, including all exons, exon/intron boundaries, and the −1.5 kb of the 5′ flanking region. Two common loci (minor allele frequency > 0.05) were genotyped in 775 T2DM patients and 316 control subjects recruited in the Korean T2DM Study.</P><P>Results </P><P>Eight polymorphisms, including four non-synonymous forms, were identified in <I>CD14</I>. No polymorphisms were found in association with T2DM. However, one common promoter SNP (<I>−260T>C</I>) was significantly associated with both the serum triglyceride level (TG) and body mass index (BMI) in non-diabetic control subjects. Individuals who carried the minor allele (C) had higher TG levels (1.65 ± 0.81 vs. 1.46 ± 0.80 mmol/l; <I>P</I> = 0.0007) and BMI (23.96 ± 3.00 vs. 23.28 ± 3.22 kg/m<SUP>2</SUP>; <I>P</I> = 0.04) as compared with subjects carrying T/T genotypes.</P><P>Conclusion </P><P>Our data suggest that lipid metabolism and obesity, important pathophysiological elements of T2DM and the metabolic syndrome, are regulated by complex mechanisms that include the CD14 gene polymorphism-mediated genetic propensity to non-specific inflammatory responses.</P>

Growth Performance and Carcass Characteristics of Korean Native Ducks Fed Diets with Varying Levels of Limiting Amino Acids

Choo, Y.K.,Kwon, H.J.,Oh, S.T.,Kang, C.W.,Kim, H.K.,Hong, E.C.,Heo, K.N.,Lee, S.K.,An, B.K. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

There are multiple experiments conducted with male Korean native ducks (KND) to evaluate the optimal levels of limiting amino acids (AA). In Exp. 1, a total of 450 one-d-old male KNDs were divided into five groups with six replicates and fed experimental diets with varying levels of lysine, total sulfur amino acids (TSAA) and threonine (T1, 0.90/0.74/0.70%; T2, 1.00/0.82/0.77%; T3, 1.10/0.90/0.85%; T4, 1.20/0.98/0.93%; T5, 1.30/1.07/1.01%) to 21 d of age. In Exp. 2, one-d-old male KND were received and fed commercial starter diet from hatching to 21 d of age, and then divided into five groups with six replicates and fed one of five diets varying levels of lysine, TSAA, and threonine (T1, 0.73/0.62/0.54%; T2, 0.80/0.68/0.60%; T3, 0.87/0.74/0.65%; T4, 0.94/0.80/0.70%; T5, 1.01/0.86/0.75%) during 22 to 56 d of age, respectively. The BW gain was linearly increased as dietary limiting AA levels increased to 1.20% lysine, 0.98% TSAA and 0.93% threonine. There were no significant differences in feed intake, gain:feed and uniformity among groups. In Exp. 2, the BW gain and gain:feed were not affected by dietary limiting AA levels. There were no significant differences in carcass characteristics and meat quality among groups. The growth performance and carcass characteristics did not show the significant response to increasing dietary limiting AA levels in KND during 22 to 56 d of age. In conclusion, the levels of lysine, TSAA and threonine necessary to maximize growth for starter phase were at least 1.20%, 0.98%, and 0.93%, respectively. On the other hands, KND require relatively low levels of limiting AA for late growth and carcass yield. The dietary levels of 0.73% lysine, 0.62% TSAA and 0.54% threonine appear to be adequate during growing phase.