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Oligomeric Structure of the ATP-dependent Protease La (Lon) of Escherichia coli
박성철,Baolei Jia,양재경,Duyet Le Van,Yong Gi Shao,한상우,전영주,정진하,정강원 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1
Lon, also known as protease La, belongs to a class of ATP-dependent serine protease. It plays an essential role in degradation of abnormal proteins and of certain short-lived regulatory proteins, and is thought to possess a Ser-Lys catalytic dyad. To examine the structural organization of Lon, we performed an electron microscope analysis. The averaged images of Lon with end-on orientation revealed a six-membered, ringshaped structure with a central cavity. The side-on view showed a two-layered structure with an equal distribution of mass across the equatorial plane of the complex. Since a Lon subunit possesses two large regions containing nucleotide binding and proteolytic domains, each layer of the Lon hexamer appears to consist of the side projections of one of the major domains arranged in a ring. Lon showed a strong tendency to form hexamers in the presence of Mg2+, but dissociated into monomers and/or dimers in its absence. Moreover, Mg2+-dependent hexamer formation was independent of ATP. These results indicate that Lon has a hexameric ring-shaped structure with a central cavity, and that the establishment of this configuration requires Mg2+, but not ATP.
$Ar^+$ RF 플라즈마 처리조건이 임베디드 PCB내 전극 Cu박막과 ALD $Al_2O_3$ 박막 사이의 계면파괴에너지에 미치는 영향
박성철,이장희,이정원,이인형,이승은,송병익,정율교,박영배,Park, Sung-Cheol,Lee, Jang-Hee,Lee, Jung-Won,Lee, In-Hyung,Lee, Seung-Eun,Song, Byoung-Ikg,Chung, Yul-Kyo,Park, Young-Bae 한국마이크로전자및패키징학회 2007 마이크로전자 및 패키징학회지 Vol.14 No.1
임베디드 PCB 기판내 유전체 재료인 Atomic Layer Deposition(ALD) $Al_2O_3$ 박막과 전극재료인 스퍼터 증착된 Cu박막 사이의 계면접착력을 $90^{\circ}$ 필 테스트방법으로 측정하여 순수 빔 굽힘을 가정한 에너지 평형 해석을 통하여 계면파괴에너지를 구하였다. $Cu/Al_2O_3$의 계면파괴에너지(${\Gamma}$)는 매우 약하여 측정할 수 없었으나, 접착력 향상층 Cr 박막을 삽입하여 $Cr/Al_2O_3$의 계면파괴에너지는 $10.8{\pm}5.5g/mm$를 얻었다. $Al_2O_3$ 표면에 $0.123W/cm^2$ 의 power density로 2분간 $Ar^+$ RF 플라즈마 전처리를 하고 Cr박막을 삽입한 $Cr/Al_2O_3$ 계면파괴에너지는 $39.8{\pm}3.2g/mm$으로 매우 크게 증가하였는데, 이는 $Ar^+$ RF 플라즈마 전처리에 따른 mechanical interlocking효과와 Cr-O 화학결합 효과가 동시에 기여한 것으로 생각된다. Interfacial fracture energy(${\Gamma}$) between $Al_2O_3$ thin film deposited by Atomic Layer Deposition(ALD) and sputter deposited Cu electrode for embedded PCB applications is measured from a $90^{\circ}$ peel test. While the interfacial fracture energy of $Cu/Al_2O_3$ is very poor, Cr adhesion layer increases the interfacial fracture energy to $39.8{\pm}3.2g/mm\;for\;Ar^+$ RF plasma power density of $0.123W/cm^2$, which seems to come from the enhancement of the mechanical interlocking and Cr-O chemical bonding effects.
박성철,안유정,송병준,조진일,Park, Sung-Chul,Ahn, Yoo-Jeong,Song, Byung-Joon,Cho, Jin-ll 한국교육녹색환경연구원 2017 교육·녹색환경연구 Vol.16 No.2
본 연구는 교육부, 학교현장, 건축가, 기업 등 관련 기관의 정책수립, 운영계획수립, 시설건립 등 다양한 사업추진과 밀접한 관계가 있는 '안전체험교실 시설모형을 개발'하기 위하여 안전체험교실에 적용하기 위한 필수 교육프로그램을 도출하고 단위공간 조성과 이에 대한 구성요소 및 활용방안을 제시하는데 목적이 있다. 본 연구는 5단계로 내용을 구성하였다. 첫째, 안전교육 수행 및 시설계획 기본방향과 관련한 문헌을 분석하였으며, 둘째, 공간규모, 시설구성 등 공간조성 실태와 운영실태분석을 바탕으로 안전교육 체험교실 실태를 조사하였다. 이를 기반으로 기존 안전체험교실의 장 단점을 분석하고 델파이 조사 및 전문가 참여디자인을 통하여 5가지의 시설모형(안)을 개발하였다. 최종적으로 개발된 안전체험교실 시설모형(안)은 1) 교통안전(보행안전, 차량안전, 지하철안전) 2) 응급처치(응급구조, 신고요령) 3) 재난안전(화재 대피안전, 지진 생활안전) 4) 승강기안전(승강기안전, 에스컬레이터안전) 5) 약물 및 폭력안전(흡연 음주안전, 성폭력안전, 식품안전)에 대한 체험존과 각 영역의 세부 안전체험공간이다. 이러한 과정을 통해 개발된 안전체험교실 시설모형(안)은 학교현장의 수용 가능한 공간적 규모에 따라 각 모형을 조합 또는 분리하여 안전체험교실을 조성할 수 있을 것이다. The purpose of this study is to derive education programs for safety training class, create unit spaces and present components and methods of utilizing the spaces for the development of facilities models closely related to various policy, operation plan and facility construction projects promoted by related institutions such as the Ministry of Education, schools, architects and companies. This study is divided into five steps. First, we reviewed the literature related basic directions for safety education and facility plan, second, field survey included both field conditions such as spatial size and facility configuration and analysis of operating conditions like hours of operation and personnel. Base on literature review and field survey, it were used to analyze strengths and weaknesses of existing safety training classes, and five facility models was developed based on the Delphi method and expert participatory design. The result show that the facility models (drafts) of safety training class were developed as follows: (1)the facility model for traffic safety(pedestrian safety, vehicle safety, subway safety) (2)the facility model for first aid(emergency rescue, how to report) (3)the facility model for disaster safety(fire evacuation safety, life earthquake safety) (4)the facility model for elevator safety(elevator safety, escalator safety) (5)the facility model for drugs and violence safety (smoking drinking, sexual harassment safety, food safety) The safety training class can be composed by combining or separating each module according to affordable space size of each school.