Risk Factors of Rehemorrhage in Postoperative Patients with Spontaneous Intracerebral Hemorrhage : A Case-Control Study

Ren, Yanming,Zheng, Jun,Liu, Xiaowei,Li, Hao,You, Chao The Korean Neurosurgical Society 2018 Journal of Korean neurosurgical society Vol.61 No.1

Objective : Rehemorrhage is the most severe complication of postoperative patients with spontaneous intracerebral hemorrhage. The aim of the present study was to assess independent predictors of rehemorrhage and find the possibility of preventing rehemorrhage in postoperative patients with spontaneous intracerebral hemorrhage (sICH). Methods : Medical records of 263 postoperative patients with sICH from our Hospital were reviewed. The relationships between rehemorrhage and parameters were examined by univariate and multivariate analyses. The parameters include time from onset to surgery, hematologic paremeters, neuroimaging characteristics, level and variability of systolic blood pressure, medical histories, operation duration, and blood loss. In addition, relationship between rehemorrhage and clinical outcome were analyzed by using multivariate analyses. Results : Thirty-five (13.31%) patients experienced rehemorrhage after operation. Multivariate analyses indicated that the following factors were independently associated with rehemorrhage : history of diabetes mellitus (odds ratio [OR], 2.717; 95% confidence interval [CI], 1.005-7.346; p=0.049), and midline shift (for every 1 mm increase, OR, 1.117; 95% CI, 1.029-1.214; p=0.009). Rehemorrhage was an independent risk factor of poor functional outcome (OR, 3.334; 95% CI, 1.094-10.155; p=0.034). Conclusion : Our finding revealed that history of diabetes mellitus and admission midline shift were possibly associated with rehemorrhage in postoperative patients with sICH.

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

Liu, Wei-Jun,Ren, Jian-Guo,Li, Ting,Yu, Guo-Zheng,Zhang, Jin,Li, Chang-Sheng,Liu, Zhi-Su,Liu, Quan-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

Mangiferin loaded magnetic PCEC microspheres: preparation, characterization and antitumor activity studies in vitro

WenJing Xiao,Jun Hou,Jie Ma,BoTao Yu,JianDong Ren,WeiHua Jin,Juan Wu,DeZhi Zheng,KaiHua Fan 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.8

Mangiferin is a promising effective chemopreventiveagent against various tumors. However, itsclinical use is limited by poor water solubility and lowbioavailability. In this article, mangiferin loaded magneticPCEC microspheres (MG-MS) were designed, characterizedand the antitumor activity of MG-MS was evaluatedin vitro. The magnetic nanoparticles (MNP) were synthesizedvia the high-temperature reaction of ironacetylacetonate in phenyl ether in the presence of oleic acidand oleylamine. Poly (ε-caprolactone)-poly (ethyleneglycol)-poly (ε-caprolactone) (PCL-PEG-PCL, PCEC)copolymers were formed by ring-opening copolymerizationof ε-CL initiated by PEG-diol using Sn(Oct)2 as acatalyst and MG-MS were prepared by solvent diffusionmethod. MNP, PCEC copolymer, and MG-MS werecharacterized by GPC, TEM, XRD, FT-IR, 1H-NMP andMalvern Laser Particle Sizer. Meanwhile, the antiproliferativeactivity in vitro and in vitro release behavior of thismicrospheres were studied in detail. The results indicatethat the obtained magnetic microspheres might have greatpotential as an effective carrier for mangiferin used incancer chemotherapy.

Phase separation phenomena and thermodynamics of octylphenol polyoxyethylene ether in micellar solutions: Effect of high concentration of inorganic salts and linear correlation

Dan Ni Li,Jing Huang,Zhao Hua Ren,Rui Sheng,Zheng Bo Qian,Bei Bei Li,Xiao Feng Quan,Ye Xi Zhang,Jun Ru Wang,Huan Tian 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

The phase separationphenomena or cloud points (CP) behaviorof nonionic octylphenol polyoxyethylene etherwith oxyethylene glycol ether unit of 15 (OP-15) were investigated in aqueous micellar solutions containinghigh concentrations of inorganic salts including NaCl, KCl, MgCl2, CaCl2, BaCl2, AlCl3, FeCl3, Na2SO4and Na2SiO3. The change in CP induced by salts and its mechanism are discussed theoretically. In aqueous solutionscontaining high concentration of salt, for thefirst time the linear relationship between CP and saltconcentrationiswellestablishedtocharacterizethedepressionofCP.Basedonpseudophaseseparationmodel,thermodynamic parameters are estimated and adopted to further describe the phase separation process andthe interactions between OP-15 molecules or the OP-15/water interactions. Thermodynamic parametersincluding standard Gibbs energy change (DG0CP), enthalpy change (DH0CP) and entropy change (DS0CP)indicate that the additions of different salts result in the change in entropy-driven nonspontaneousprocess of phase separation. These data, which are from the compensation temperatures and the changeof molar heat capacity, also prove that different ions induce the change in the interactions betweensurfactant molecules or the surfactant–water interaction and thus affect the depression of CP. Thesefindings help with understanding the effect of inorganic salts, especially the high concentration of salt, onCP of nonionic surfactant and then broadening the application of nonionic surfactant based on CP such asthe extraction of biomaterials in biological samples derived from animals or plants, etc.

Analysis and Design of a PFC AC–DC Converter with Electrical Isolation

Chia-Ching Lin,Lung-Sheng Yang,Ren-Jun Zheng 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

This study presents a single-phase power factor correction AC?DC converter that operates in discontinuous conduction mode. This converter uses the pulse-width modulation technique to achieve almost unity power factor and low total harmonic distortion of input current for universal input voltage (90 Vrms to 264 Vrms) applications. The converter has a simple structure and electrical isolation. The magnetizing-inductor energy of the transformer can be recycled to the output without an additional third winding. The steady-state analysis of voltage gain and boundary operating conditions are discussed in detail. Finally, experimental results are shown to verify the performance of the proposed converter.

Effects of different modification techniques on molecular structure and bioactivity of Bombyx mori pupa protein

Zhi-Feng Zhou,Zi-Xu Ren,Hai-Yan Yu,Jun-Qiang Jia,Zhong-Zheng Gui 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

To improve the properties of silkworm (Bombyx mori) pupa protein (SPP), the effects of different modification techniques included enzymolysis, ultrasound, and micronization on the molecular structure and bioactivity of SPP has been evaluated in this study. The results showed that enzymolysis modification led to the most substantial change in the area and shape of the infrared (IR) absorption peaks, decreased in size with an increased number of cracks of modified protein by scanning electron microscopy images, followed by ultrasound and micronization modification. After modification by enzymolysis, ultrasound, and micronization, the sulfhydryl concentrations of SPPwere increased by 48.56%, 34.82%, and 12.46%, respectively; disulfide bond concentrations were reduced by 40.47%, 10.79%, and 11.72%, respectively. Furthermore, the ratios of essential amino acids among total amino acids increased by 13.85%, 2.22%, and 6.93%, respectively. Antioxidant activity and ACE-inhibitory activity of SPP also increased notably after modifications.

Analysis and Design of a PFC AC-DC Converter with Electrical Isolation

Lin, Chia-Ching,Yang, Lung-Sheng,Zheng, Ren-Jun The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.5

This study presents a single-phase power factor correction AC-DC converter that operates in discontinuous conduction mode. This converter uses the pulse-width modulation technique to achieve almost unity power factor and low total harmonic distortion of input current for universal input voltage $90V_{rms}$ to $264V_{rms}$) applications. The converter has a simple structure and electrical isolation. The magnetizing-inductor energy of the transformer can be recycled to the output without an additional third winding. The steady-state analysis of voltage gain and boundary operating conditions are discussed in detail. Finally, experimental results are shown to verify the performance of the proposed converter.

Identification and characterization of a novel endoglucanase (CMCase) isolated from the larval gut of Bombyx mori

Ru-Jian Ma,Chun-Yan Wang,Yan-Wei Liu,Thasma Raman Sivakumar,Zi-Xu Ren,Ying Fang,Jun-Qiang Jia,Zhong-Zheng Gui 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1

While screening for cellulase-producing fungi from insect gut, a fungus with high endoglucanase (carboxymethylcellulase; CMCase) activitywas isolated fromthe larval gut of Bombyx mori. Based onmorphological characteristicsand using an 18S rRNA-based molecular phylogenetic approach, the fungus, strain BMC-2, was identified as aMucor sp. expressing a novel alkalotolerant cellulase. The maximum production of cellulase by the BMC-2 strainwas observed at 55°C and pH8.0. The CMCase activity was inhibited by Cu2+ N Na+ N Zn2+ N Mg2+ N Ba2+, andinduced by Ca2+, Mn2+, Fe2+, and K+.

Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.