Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

( Jia-jun Xie ),( Xu Chen ),( Jin Niu ),( Jun Wang ),( Yun Wang ),( Qiang-qiang Liu ) 한국수산과학회 2017 Fisheries and Aquatic Sciences Vol.20 No.2

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and H₂O₂ supplementation were applied (control group: without both astaxanthin and H₂O₂ treated; H₂O₂ group: just with H₂O₂ treated; H₂O₂ + AST group: with both astaxanthin and H₂O₂ treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of H₂O₂ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation (H₂O₂ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from H₂O₂ (P < 0.05), but it could be raised by astaxanthin supplementation (H₂O₂ vs H₂O₂ + AST), and the malondialdehyde (MDA) was significant higher in H₂O₂ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

Expression of Connexin 43 and E-cadherin Protein and mRNA in Non-small Cell Lung Cancers in Chinese Patients

Zhao, Jun-Qiang,Sun, Fang-Jie,Liu, Shan-Shan,Yang, Jun,Wu, Yu-Quan,Li, Gui-Shan,Chen, Qing-Yong,Wang, Jia-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Aim: Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC). Methods: Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR. Results: (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis. Conclusion: The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.

Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

Xie, Jia-jun,Chen, Xu,Niu, Jin,Wang, Jun,Wang, Yun,Liu, Qiang-qiang The Korean Society of Fisheries and Aquatic Scienc 2017 Fisheries and Aquatic Sciences Vol.20 No.4

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and $H_2O_2$ supplementation were applied (control group: without both astaxanthin and $H_2O_2$ treated; $H_2O_2$ group: just with $H_2O_2$ treated; $H_2O_2$ + AST group: with both astaxanthin and $H_2O_2$treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of $H_2O_2$ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation ($H_2O_2$ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from $H_2O_2$ (P < 0.05), but it could be raised by astaxanthin supplementation ($H_2O_2$ vs $H_2O_2$ + AST), and the malondialdehyde (MDA) was significant higher in $H_2O_2$ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

Iso-suillin Isolated from Suillus luteus, Induces G₁ Phase Arrest and Apoptosis in Human Hepatoma SMMC-7721 Cells

Jia, Zhi-Qiang,Chen, Ying,Yan, Yong-Xin,Zhao, Jun-Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Iso-suillin, a natural product isolated from Suillus luteus, has been shown to inhibit the growth of some cancer cell lines. However, the molecular mechanisms of action of this compound are poorly understood. The purpose of this study was to investigate how iso-suillin inhibits proliferation and induces apoptosis in a human hepatoma cell line (SMMC-7721). We demonstrated the effects of iso-suillin on cell proliferation and apoptosis in SMMC-7721 cells, with no apparent toxicity in normal human lymphocytes, using colony formation assays and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Western blotting was used to examine the expression of $G_1$ phase-regulated and apoptosis-associated protein levels in iso-suillin treated SMMC-7721 cells. The results indicated that iso-suillin significantly decreased viability, induced $G_1$ phase arrest and triggered apoptosis in SMMC-7721cells. Taken together, these results suggest the potential of iso-suillin as a candidate for liver cancer treatment.

Comparison of Liver Transplantation and Liver Resection for Hepatocellular Carcinoma Patients with Portal Vein Tumor Thrombus Type I and Type II

Jia-Yu Lv,Ning-Ning Zhang,Ya-Wei Du,Ying Wu,Tian-Qiang Song,Ya-Min Zhang,Yan Qu,Yu-Xin Liu,Jie Gu,Ze-Yu Wang,Yi-Bo Qiu,Bing Yang,Da-Zhi Tian,Qing-Jun Guo,Li Zhang,Ji-San Sun,Yan Xie,Zheng-Lu Wang,Xin 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.1

Purpose: The aim of this study was to compare the efficacy of liver transplantation (LT) and liver resection (LR) for hepatocellularcarcinoma (HCC) patients with portal vein tumor thrombus (PVTT) and to investigate risk factors affecting prognosis. Materials and Methods: A total of 94 HCC patients with PVTT type I (segmental PVTT) and PVTT type II (lobar PVTT) were involvedand divided into LR (n=47) and LT groups (n=47). Recurrence-free survival (RFS) and overall survival (OS) were comparedbefore and after inverse probability of treatment weighting (IPTW). Prognostic factors for RFS and OS were explored. Results: Two treatment groups were well-balanced using IPTW. In the entire cohort, LT provided a better prognosis than LR. Among patients with PVTT type I, RFS was better with LT (p=0.039); OS was not different significantly between LT and LR(p=0.093). In subgroup analysis of PVTT type I patients with α-fetoprotein (AFP) levels >200 ng/mL, LT elicited significantly longermedian RFS (18.0 months vs. 2.1 months, p=0.022) and relatively longer median OS time (23.6 months vs. 9.8 months, p=0.065). Among patients with PVTT type II, no significant differences in RFS and OS were found between LT and LR (p=0.115 and 0.335,respectively). Multivariate analyses showed treatment allocation (LR), tumor size (>5 cm), AFP and aspartate aminotransferase(AST) levels to be risk factors of RFS and treatment allocation (LR), AFP and AST as risk factors for OS. Conclusion: LT appeared to afford a better prognosis for HCC with PVTT type I than LR, especially in patients with AFP levels>200 ng/mL.

Phenolic composition and nutritional attributes of diaphragma juglandis fructus and shell of walnut (Juglans regia L.)

Qiang Hu,Jia Liu,Jun Li,Hui Liu,Nan Dong,Yang-yang Geng,Yang Lu,Yan Wang 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.2

Phenolic composition and nutritional attributes of diaphragma juglandis fructus (Djf) and walnut shells (Ws) were investigated. Phenolic acids, hydroxybenzoic acid, isoflavone, and flavone were identified in the free phenolic fractions (FPFs) of both Djf and Ws. Bound phenolic fractions were less than FPFs both in content and diversity. The soluble dietary fiber contents of Djf and Ws were 25.56 g/100 g and 9.5 g/100 g, respectively. The contents of unsaturated fatty acids (1912.28 mg/kg and 9137.56 mg/kg, respectively) were significantly higher than that of saturated fatty acid both in Djf and Ws. The content of essential amino acids in Djf (9.67 mg/g) was significantly higher than that in Ws. More than eight types of monosaccharides were detected in Djf and Ws. The monosaccharide content of Djf (314.16 mg/g) was significantly higher than that of Ws (60.97 mg/g). Trehalose was the predominant component both in Djf (71.2%) and Ws (78.6%).

The Dynamic Mechanical Properties for Recycled Aggregate Concrete under Tensile-Compressive States

Zhen-Jun He,Zhi-qiang Wang,Meng-jia Ding,Zhen-wei Wang,Xiao-jie Zhang,Lian-shuo Liu 대한토목학회 2020 KSCE Journal of Civil Engineering Vol.24 No.5

The experiments under biaxial tensile-compressive (T-C) complex stress were carried out for two kinds of substitution percentages of recycled coarse aggregate (RCA) on biaxial proportional loading (α = σ1:σ3 =1:0, 0: -1, 0.05: -1, 0.1: -1, 0.25: -1, 0.5: -1, 1: -1 and-1: -1) and four loading deformation rates of 10−5/s, 10−4/s , 10−3/s, and 10−2/s. The research was completed in the static-dynamic true triaxial machine. The experimental phenomena indicate the tensile failure appears in these specimens of recycled aggregate concrete (RAC) under biaxial T-C stress states. The dynamic mechanical behavior for RAC under biaxial T-C loadings was affected by the strain rates, stress ratios and its substitution percentages of RCA. Accompanied by the increase of strain rates, its strength of RAC is improved. As the tensile stress increases, its compressive strength decreases. The T-C strengths for RAC under biaxial T-C states are less than its corresponding uniaxial T-C strengths at a certain identical strain rates, respectively. Considering the effect of loading rates, a new tensile-compressive failure criterion is established under complex stress states.

Distinctions Between Clinicopathological Factors and Prognosis of Alpha-fetoprotein Negative and Positive Hepatocelluar Carcinoma Patients

Xu, Jia,Liu, Chang,Zhou, Lei,Tian, Feng,Tai, Ming-Hui,Wei, Ji-Chao,Qu, Kai,Meng, Fan-Di,Zhang, Ling-Qiang,Wang, Zhi-Xin,Zhang, Jing-Yao,Chang, Hu-Lin,Liu, Si-Nan,Xu, Xin-Shen,Song, Yan-Zhou,Liu, Jun,Z Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

Serum alpha-fetoprotein (AFP) is a significant marker for clinical diagnosis and prognosis evaluation in hepatocellular carcinoma (HCC) patients. However, some proportion of liver cancer patients are AFP-negative (AFP ${\leq}$20ng/ml). In order to study the differences between clinicopathological factors and prognosis of alpha-fetoprotein negative and positive patients, a total of 114 cases (41 AFP-negative and 73 AFP-positive) were selected for our research. By systematically statistical analysis, the results demonstrated that compared with AFP-negative patients, AFP-positive examples were more likely to feature cirrhosis nodules, non-complete neoplasm capsules, and a poor Edmondson-steiner grade. Furthermore, AFP-negative patients demonstrated a favorable long-term prognosis. By univariate analysis and multivariate analysis with Cox's proportional hazards model, multiple tumors were found to be independent risk factors for worse survival of AFP negative patients; however, less tumor-free margins, multiple tumors and Edmondson-steiner grades III/IV, proved to be independent risk factors leading to a poor prognosis of AFP positive cases. Finally, we can infer that high levels of AFP signify a highly malignant tumor and unfavorable prognosis.

Study on Safety of Up-righting Project for a Capsized Ship Model Based on Cable Tensions, Theoretical Model and NumericalMethod Accounting for Dynamic Effect

Yu-Qiang Sun,De-Jun Jia 국제이네비해양경제학회 2018 International Journal of e-Navigation and Maritime Vol.10 No.1

During an up-righting project for a capsized ship, the dynamic effect enlarges the tensions of cables. The cable tensions which are calculated based on statics and the safety evaluation which is based on these tensions cannot ensure the safety of the up-righting project. Due to the above reasons, a numerical simulation project is applied to investigate the dynamic effect on cable tensions of up-righting projects and evaluate the safety of up-righting project in current research. Firstly, a theoretical equation of the quasi-static up-righting project model is established in current research and is solved. Subsequently, the precision of the numerical simulation method applied in current research is checked by comparing cable tensions which are calculated by theoretical model and numerical simulation method. The cable tensions in different cases are solved by the numerical simulation method which accounts for dynamic effect in order to investigate the relationship between cable tensions and capsized ship weights, cable stiffness and winding-in speed. Finally, the safety of up-righting project is evaluated based on all the cable tensions calculated. It is pointed out that cable tension increases with the value of capsized ship weight, cable stiffness or winding-in speed approximately. The safety coefficients of cable in some high winding-in speeds are bellow 1.0. The results indicate that the dynamic effect is significant and should be accounted for during the up-righting project.

Effect of hanging-type sand fence on characteristics of wind-sand flow fields

Jian-jun Cheng,Jia-qiang Lei,Sheng-yu Li,Hai-feng Wang 한국풍공학회 2016 Wind and Structures, An International Journal (WAS Vol.22 No.5

A hanging-type sand-retaining wall is a very common sand-blocking fence structure used to prevent sand movement. This type of wall is widely used along the Qinghai–Tibet and Gobi desert railways in Xinjiang, Western China. To analyze the characteristics of wind-sand flow fields under the effect of such a sand fence structure, a wind tunnel test and a field test were carried out. The wind tunnel test showed the zoning characteristics of the flow fields under the effect of the hanging-type sand-retaining wall, and the field test provided the sediment transport data for effective wind-proof interval and the sand resistance data in the front and behind the sand-retaining wall. The consistency of the wind-sand flow fields with the spatial distribution characteristic of wind-carried sand motion was verified by the correspondences of the acceleration zone in the flow field and the negative elevation points of the percentage variations of the sand collection rate. The spatial distribution characteristic of the field sand collection data further showed the spatial structural characteristic of the sandy air currents under the action of the hanging-type sand-retaining wall and the sand resistance characteristic of the sand-retaining wall. This systematic study on the wind-sand flow fields under the control of the hanging-type sand-retaining wall provides a theoretical basis for the rational layout of sand control engineering systems and the efficient utilization of a hanging-type sand-retaining wall.