Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.

A Resilience Model for Engineered Slopes Subject to Anchor Corrosion

Xue-You Li,Zeng-Bin Fan,Tao Lu,Te Xiao,Li-Min Zhang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.3

Many high engineered slopes are stabilized using anchors, which may corrode over time. Proper maintenance is essential to recover the system performance and upkeep slope functions. This paper presents a resilience model for maintenance decision by analysing the degradation of an anchor-stabilized slope due to corrosion and evaluating the recovery of slope performance after maintenance. Failure probability is used as an indicator to characterize the evolution of slope performance within its service life. The timing for maintenance is determined when the failure probability reaches an intolerable value. Information obtained from regular inspections is used to reduce the uncertainty in the determined maintenance time. The effectiveness of possible repair schemes is measured by a resilience index for the slope attained after maintenance, which considers the reduction in the failure probability and the duration over which the failure probability remains acceptable. A benefit index, which incorporates both the effectiveness and cost of a repair measure, is defined and used to quantitatively evaluate possible maintenance schemes. The proposed model is illustrated using an anchor-stabilized rock slope example, which provides a means to quantitatively assess different repair measures and enables engineers to select an effective and economic measure for slope maintenance.

Meta-analysis of Excision Repair Cross-complementation Group 1 (ERCC1) Association with Response to Platinum-based Chemotherapy in Ovarian Cancer

Li, Feng-Ying,Ren, Xiao-Bin,Xie, Xin-You,Zhang, Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Recent studies suggested that the ovarian cancers with negative excision repair cross-complementation group 1 enzyme (ERCC1) expression have a better response to platinum-based chemotherapy than those with positive ERCC1 expression. The objective of this study was to evaluate whether ERCC1 expression is associated with response to platinum-based chemotherapy in ovarian cancers. MEDLINE, PubMed, Web of Science and CNKI databases were used for searching studies relating to ERCC1 protein expression and response to platinum-based chemotherapy in ovarian cancers. Statistical analysis was based on the method for a fixed effects meta-analysis. Pooled odds ratios (ORs) with 95% confidence intervals for ERCC1 protein expression and response to platinum-based chemotherapy were generated. Publication bias was investigated with Begg's test. Five studies involving 306 patients with ovarian cancer were included. Compared to patients with positive ERCC1 expression, those with negative ERCC1 expression had a better response to platinum-based chemotherapy. The pooled OR was 5.264 (95% CI: 2.928-9.464, P < 0.001) and publication bias was not found (P = 0.904). The result was similar in both in Asians and Caucasians (P < 0.001 and P = 0.028, respectively). ERCC1 protein expression status is significantly associated with response to platinum-based chemotherapy in ovarian cancers.

Dynamic analysis of wind-vehicle-bridge systems using mutually-affected aerodynamic parameters

Bin Wang,You-Lin Xu,Yongle Li 한국풍공학회 2015 Wind and Structures, An International Journal (WAS Vol.20 No.2

Several frameworks for the dynamic analysis of wind-vehicle-bridge systems were presented inthe past decade to study the safety or ride comfort of road vehicles as they pass through bridges undercrosswinds. The wind loads on the vehicles were generally formed based on the aerodynamic parameters ofthe stationary vehicles on the ground, and the wind loads for the pure bridge decks without the effects ofroad vehicles. And very few studies were carried out to explore the dynamic effects of the aerodynamicinterference between road vehicles and bridge decks, particularly for the moving road vehicles. In this study,the aerodynamic parameters for both the moving road vehicle and the deck considering themutually-affected aerodynamic effects are formulized firstly. And the corresponding wind loads on the roadvehicle-bridge system are obtained. Then a refined analytical framework of the WVB system incorporatingthe resultant wind loads, a driver model, and the road roughness in plane to fully consider the lateral motionof the road vehicle under crosswinds is proposed. It is shown that obvious lateral and yaw motions of theroad vehicle occur. For the selected single road vehicle passing a long span bridge, slight effects are causedby the aerodynamic interference between the moving vehicle and deck on the dynamic responses of thesystem.

Face inpainting via Learnable Structure Knowledge of Fusion Network

You Yang,Sixun Liu,Bin Xing,Kesen Li 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.3

With the development of deep learning, face inpainting has been significantly enhanced in the past few years. Although image inpainting framework integrated with generative adversarial network or attention mechanism enhanced the semantic understanding among facial components, the issues of reconstruction on corrupted regions are still worthy to explore, such as blurred edge structure, excessive smoothness, unreasonable semantic understanding and visual artifacts, etc. To address these issues, we propose a Learnable Structure Knowledge of Fusion Network (LSK-FNet), which learns a prior knowledge by edge generation network for image inpainting. The architecture involves two steps: Firstly, structure information obtained by edge generation network is used as the prior knowledge for face inpainting network. Secondly, both the generated prior knowledge and the incomplete image are fed into the face inpainting network together to get the fusion information. To improve the accuracy of inpainting, both of gated convolution and region normalization are applied in our proposed model. We evaluate our LSK-FNet qualitatively and quantitatively on the CelebA-HQ dataset. The experimental results demonstrate that the edge structure and details of facial images can be improved by using LSK-FNet. Our model surpasses the compared models on L1, PSNR and SSIM metrics. When the masked region is less than 20%, L1 loss reduce by more than 4.3%.

Evaluation of Non-isothermal Melt Crystallization Kinetics of the Poly(lactic acid)/Graphene Composites under Variant Cooling Circumstances via Solidification Analysis Approach

Bin Yang,Peng Zhang,Shu-Chun Zhao,Ru Xia,Li-Fen Su,Ji-Bin Miao,Peng Chen,Jia-Sheng Qian,You Shi 한국고분자학회 2019 폴리머 Vol.43 No.5

The poly(lactic acid) (PLA)/graphene (GN) composites were prepared by solution blending. An in situ measurement technique was adopted to examine the non-isothermal crystallization of PLA/GN composites, which was different from the typical circumstances at constant cooling rate in DSC. The rheological properties of the samples were investigated, and the Carreau-A model was applied to non-linearly fit the rheological data. The non-isothermal crystallization kinetics and solidification behaviors at variant cooling rates were investigated using a modified version of Avrami equation by Jeziorny coupled with three-parameter model (TPM) and four-parameter model (FPM). Our findings showed that the viscosity of the PLA/GN composites dropped with increasing shear rate or GN content. Crystallization kinetic analysis suggested that GN served as an effective nucleating agent for PLA under non-isothermal melt crystallization conditions with variant cooling rate.

Crosswind effects on high-sided road vehicles with and without movement

Bin Wang,You-Lin Xu,Le-Dong Zhu,Yong-Le Li 한국풍공학회 2014 한국풍공학회지 Vol.18 No.2

The safety of road vehicles on the ground in crosswind has been investigated for many years. One of the most important fundamentals in the safety analysis is aerodynamic characteristics of a vehicle in crosswind. The most common way to study the aerodynamic characteristics of a vehicle in crosswind is wind tunnel tests to measure the aerodynamic coefficients and/or pressure coefficients of the vehicle. Due to the complexity of wind tunnel test equipment and procedure, the features of flow field around the vehicle are seldom explored in a wind tunnel, particularly for the vehicle moving on the ground. As a complementary to wind tunnel tests, the numerical method using computational fluid dynamics (CFD) can be employed as an effective tool to explore the aerodynamic characteristics of as well as flow features around the vehicle. This study explores crosswind effects on a high-sided lorry on the ground with and without movement through CFD simulations together with wind tunnel tests. Firstly, the aerodynamic forces on a stationary lorry model are measured in a wind tunnel, and the results are compared with the previous measurement results. The CFD with unsteady RANS method is then employed to simulate wind flow around and wind pressures on the stationary lorry. The numerical aerodynamic forces are compared with the wind tunnel test results. Furthermore, the same CFD method is extended to investigate the moving vehicle on the ground in crosswind. The results show that the CFD results match with wind tunnel test results and the current way using aerodynamic coefficients from a stationary vehicle in crosswind is acceptable. The CFD simulation can provide more insights on flow field and pressure distribution which are difficult to be obtained by wind tunnel tests.

RhoGDI2 induced malignant phenotypes of pancreatic cancer cells via regulating Snail expression

Yi Bin,Hu You,Zhu Dongming,Yao Jun,Zhou Jian,Zhang Yi,He Zhilong,Zhang Lifeng,Zhang Zixiang,Yang Jian,Tang Yuchen,Huang Yujie,Li Dechun,Liu Qiuhua 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: Rho GDP dissociation inhibitor 2 (RhoGDI2) has been shown to contribute to the aggressive phenotypes of human cancers, such as tumor metastasis and chemoresistance. Objective: This study aimed to assess the effects of RhoGDI2 on tumor progression and chemoresistance in pancreatic cancer cells. Methods: The expression of RhoGDI2 in pancreatic cancer cells was detected by Western blot analysis. Gain-of-function and loss-of-function approaches were done to examine the malignant phenotypes of the RhoGDI2-expressing or RhoGDI2-depleting cells. The correlation between RhoGDI2 and Snail was also analyzed. Results: Differential expression of RhoGDI2 protein in pancreatic cancer cell lines was identified. Gain-of-function and loss-of-function experiments showed that RhoGDI2 induced the malignant phenotypes of pancreatic cancer cells, including proliferation, migration, invasion, and gemcitabine (GEM) chemoresistance. The upregulation of RhoGDI2 stimulated the expression of Snail, resulting in the altered expression of epithelial marker E-cadherin and mesenchymal marker Vimentin, which were characteristics of the tumorigenic activity of epithelial-mesenchymal transition. The expression of RhoGDI2 and Snail was upregulated in clinical tumor samples, and higher expression of RhoGDI2 or Snail was significantly associated with poor patient survival in pancreatic ductal adenocarcinoma (PDAC). Conclusion: The findings indicated that RhoGDI2 promoted GEM resistance and tumor progression in pancreatic cancer and that RhoGDI2 might be a potential therapeutic target in patients with PDAC.

Dynamic analysis of wind-vehicle-bridge systems using mutually-affected aerodynamic parameters

Wang, Bin,Xu, You-Lin,Li, Yongle Techno-Press 2015 Wind and Structures, An International Journal (WAS Vol.20 No.2

Several frameworks for the dynamic analysis of wind-vehicle-bridge systems were presented in the past decade to study the safety or ride comfort of road vehicles as they pass through bridges under crosswinds. The wind loads on the vehicles were generally formed based on the aerodynamic parameters of the stationary vehicles on the ground, and the wind loads for the pure bridge decks without the effects of road vehicles. And very few studies were carried out to explore the dynamic effects of the aerodynamic interference between road vehicles and bridge decks, particularly for the moving road vehicles. In this study, the aerodynamic parameters for both the moving road vehicle and the deck considering the mutually-affected aerodynamic effects are formulized firstly. And the corresponding wind loads on the road vehicle-bridge system are obtained. Then a refined analytical framework of the WVB system incorporating the resultant wind loads, a driver model, and the road roughness in plane to fully consider the lateral motion of the road vehicle under crosswinds is proposed. It is shown that obvious lateral and yaw motions of the road vehicle occur. For the selected single road vehicle passing a long span bridge, slight effects are caused by the aerodynamic interference between the moving vehicle and deck on the dynamic responses of the system.

A new phenylethanoid glycoside with antioxidant and anti-HBV activity from Tarphochlamys affinis

Xian-Li Zhou,Qing-Wei Wen,Xing Lin,Shi-Jun Zhang,Ying-Xin Li,You-Jia Guo,Ren Bin Huang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.5

A new phenylethanoid glycoside, named taraffinisosideA (1), together with five known glycosideswere isolated from the stems and leaves of Tarphochlamysaffinis. The structure of taraffinisoside A was identified onthe basis of detailed spectral analysis. Compounds 1–4 and6 showed potent antioxidant activities with IC50 values of10.36, 19.73, 43.95, 15.30 and 46.04 lM by 1,1-diphenyl-2-picryhydrazyl radical-scavenging assay. Compounds 1, 2and 4 showed anti-HBV activities, with IC50 values of0.50, 0.72 and 0.26 mM for HBsAg and 0.93, 0.42 and0.07 mM for HBeAg, respectively.