Rapid Detection and Monitoring Therapeutic Efficacy of Mycobacterium tuberculosis Complex Using a Novel Real-Time Assay

( Jiang Li Juan ),( Wen Juan Wu ),( Hai Wu ),( Son Sik Ryang ),( Jian Zhou ),( Wei Wu ),( Tao Li ),( Jian Guo ),( Hong Hai Wang ),( Shui Hua Lu ),( Yao Li ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ≤35 and the ratio of real-time RT-PCR and real-time PCR load was ≥1.51. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including nonrespiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

The Storytelling Rhythms of Chinese and Korean Films

WU JUAN 국제문화기술진흥원 2020 International Journal of Advanced Culture Technolo Vol.8 No.4

In literature it is not only the meaning of words which matters, but also their texture matters i.e. their rhythm, colour and style are relevant and none of these natures can be reduced to an item of information. The texture is also important in film languages, especially the rhythm. In order to make the storytelling rhythm visible, a new concept of ‘the Rhythm Chart Analysis Method (RCAM)’ has been devised in this research and used for analysis. By analyzing original films and remakes in Korean and Chinese, one can find out that different countries have different storytelling rhythms i.e. the same story can be told in different rhythms. The central idea in Korean films is portrayed at the end, which is a typical characteristic of Korean films. And as the defining moment does not occur suddenly the audience can naturally get immersed into the story. But Chinese films communicate with the audience in a more direct way. It directly mentions characteristics of each actor in such a way that it is telling rather than showing. The information of the movie is given to the audience in the initial stages of the movie. Rhythm is as important as story and information. And through this we can find out the cultural differences from the different storytelling rhythms.

Understanding of Globalization and Transnational Chinese Cinema

WU Juan 중국영화포럼 2014 영화중국 Vol.1 No.1

This year, a landmark film co-production treaty between the UK and China was signed, by looking back the Chinese cinema in UK, analysis the reasons for film co-production, this essay explores how globalization influenced Chinese cinema and what is the availability of Chinese cinema.

헤어질 결심으로 환대를 해체하기

오연(Wu Juan) 한국영화학회 2023 영화연구 Vol.- No.96

중국문화연구에 대한 상호텍스트적 접근 — 〈사천의 선인〉과 〈스틸라이프〉를 중심으로

오연(Wu, Juan) 중국문화연구학회 2021 중국문화연구 Vol.- No.53

The aim of this paper is to expand the scope of Chinese cultural studies through the perspective of intertextuality. It is intended to supplement the limitations of regional and cultural researches which have been conducted as mutual academic research, thereby conducting cultural researches on the theme of ‘relationship’. The view of this paper is based on the dialogic relationship in historical and cultural contexts of Bakhtin, and Kristeva’s intertextuality which is on the same line as dialogics. A careful analysis of two works, one by Bertolt Brecht, a wellknown German playwright, which is entitled 〈The Good Person of Szechwan(1943)〉 and the other from a Chinese film entitled 〈Still Life(2006)〉, will clearly reveal their dialogic relationship. The German playwright Bertolt Brecht used the theater as a forum for social critique. He was influenced by a wide variety of sources from Chinese, Japanese and many more. He intends to make a referential use of the advantages of Chinese drama. The play 〈The Good Person of Szechwan〉 is one of Brecht’s most intercultural plays, blending his fascinations for Chinese theatricality and German politics. Jia Zhangke, a Chinese film director who created 〈Still Life〉, was much influenced by Brecht. The flim 〈Still Life〉 is an example of this kind of Interculturalism. The background of creation is different, but the creative techniques of the two works, the power relationship, and ideology in the works are closely interlinked. Many new things can be discovered in this dialogic relationship, which would otherwise be ignored simply by concentrating on one text. Polyphony, which can be found in de-subjective, decentralized, and de-nationalized relationships, shows new possibilities for interpretation and creation. In the future, it will be necessary to pay attention to the cross-cultural landscape in which tradition, modernity, and different countries interact together to form new cultures in various genres.

Instance segmentation with pyramid integrated context for aerial objects

Juan Wang,Liquan Guo,Minghu Wu,Guanhai Chen,Zishan Liu,Yonggang Ye,Zetao Zhang 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.3

Aerial objects are more challenging to segment than normal objects, which are usually smaller and have less textural detail. In the process of segmentation, target objects are easily omitted and misdetected, which is problematic. To alleviate these issues, we propose local aggregation feature pyramid networks (LAFPNs) and pyramid integrated context modules (PICMs) for aerial object segmentation. First, using an LAFPN, while strengthening the deep features, the extent to which low-level features interfere with high-level features is reduced, and numerous dense and small aerial targets are prevented from being mistakenly detected as a whole. Second, the PICM uses global information to guide local features, which enhances the network's comprehensive understanding of an entire image and reduces the missed detection of small aerial objects due to insufficient texture information. We evaluate our network with the MS COCO dataset using three categories: airplanes, birds, and kites. Compared with Mask R-CNN, our network achieves performance improvements of 1.7%, 4.9%, and 7.7% in terms of the AP metrics for the three categories. Without pretraining or any postprocessing, the segmentation performance of our network for aerial objects is superior to that of several recent methods based on classic algorithms.

KIF11 Functions as an Oncogene and Is Associated with Poor Outcomes from Breast Cancer

Juan Zhou,Wei-Rong Chen,Li-Chao Yang,Jun Wang,Jia-Yuan Sun,Wen-Wen Zhang,Zhen-Yu He,San-Gang Wu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3

Purpose The study aimed to search and identify genes that were differentially expressed in breast cancer, and their roles in cancer growth and progression. Materials and Methods The Gene Expression Omnibus (Oncomine) and The Cancer Genome Atlas databases (https://cancergenome.nih.gov/) were screened for genes that were expressed differentially in breast cancer and were closely related to a poor prognosis. Gene expressions were verified by quantitative real-time polymerase chain reaction, and genes were knocked down by a lentivirus-based system. Cell growth and motility were evaluated and in vivo nude mice were used to confirm the in vitro roles of genes. Markers of epithelial-to-mesenchymal transition and the associations of KIF11 with the classical cancer signaling pathways were detected by Western blot. Results A series of genes expressed differentially in patients with breast cancer. The prognosis associated with high KIF11 expression was poor, and the expression of KIF11 increased significantly in high stage and malignant tumor cells. Inhibiting KIF11 expression in lentivirussuppressed cells revealed that KIF11 inhibition significantly reduced cell viability and colony formation, inhibited migration and invasion, but promoted apoptosis. The sizes and weights of KIF11-inhibited tumors in nude mice were significantly lower than in the negative controls. Western blot showed that E-cadherin in breast cancer was significantly upregulated in KIFinhibited cells and tumor tissues, whereas N-cadherin and vimentin were significantly downregulated. BT549 and MDA231 cells with KIF11 knockdown exhibited decreased ERK, AMPK, AKT, and CREB phosphorylation. Conclusion KIF11 acts as a potential oncogene that regulates the development and progression of breast cancer.

Exhaust gas based nanoarchitectonics for porous carbon materials for high-performance supercapacitor

Juan Du,Aibing Chen,Xueqing Gao,Haixia Wu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

Pyrolyzing carbon precursors will also lead to a large amount of exhaust gas, which is a waste ofresources and a huge pollution. Therefore, realizing the full utilization of exhaust gas from carbon precursorsis significant. Herein, cobalt (Co) modified hard templates with micropores or mesopores is used toprepare porous carbon for supercapacitors, in which Co species provide the active sites for the captureand deposition of exhaust gas derived from zeoliticimidazolate framework. Using N2 as protective andcarrier gas, the whole process can be realized in a simple tubular furnace. The obtained PC can replicatethe morphology and pore structure of the hard template with the characteristics of micropores or mesoporesrespectively. The microporous structure of PC gives it higher surface area and pore volume than PCwith mesopores, which makes it have high capacitance of 445.7 F g1 in supercapacitor.

Ovarian Ablation Using Goserelin Improves Survival of Premenopausal Patients with Stage II/III Hormone Receptor-Positive Breast Cancer without Chemotherapy-Induced Amenorrhea

Juan Zhou,San-Gang Wu,Jun-Jie Wang,Jia-Yuan Sun,Fengyan Li,Qin Lin,Huan-Xin Lin,Zhen-Yu He 대한암학회 2015 Cancer Research and Treatment Vol.47 No.1

Purpose The purpose of this study was to assess the value of ovarian ablation using goserelin inpremenopausal patients with stage II/III hormone receptor-positive breast cancer withoutchemotherapy-induced amenorrhea (CIA). Materials and MethodsWe retrospectively reviewed the data of breast patients treated between October 1999 andNovember 2007 without CIA. The Kaplan-Meier method was used for calculation of thesurvival rate. Log rank method and Cox regression analysis were used for univariate andmultivariate prognostic analysis. ResultsThe median follow-up period was 61 months. Initially, 353 patients remained without CIAafter chemotherapy and 98 among those who received goserelin and tamoxifen (TAM). Inunivariate analysis, goserelin improved locoregional recurrence-free survival (LRFS) (98.9%vs. 94.1%, p=0.041), distant metastasis-free survival (DMFS) (85.4% vs. 71.9%, p=0.006),disease-free survival (DFS) (85.4% vs. 71.6%, p=0.005), and overall survival (OS) (93.5%vs. 83.5%, p=0.010). In multivariate analysis, goserelin treatment was an independentfactor influencing DMFS (hazard ratio [HR], 1.603; 95% confidence interval [CI], 1.228 to2.092; p=0.001), DFS (HR, 1.606; 95% CI, 1.231 to 2.096; p=0.001), and OS (HR, 3.311;95% CI, 1.416 to 7.742; p=0.006). In addition, treatment with goserelin resulted in significantlyimproved LRFS (p=0.039), DMFS (p=0.043), DFS (p=0.036), and OS (p=0.010) inpatients aged < 40 years. In patients aged  40 years, goserelin only improved DMFS(p=0.028) and DFS (p=0.027). ConclusionOvarian ablation with goserelin plus TAM resulted in significantly improved therapeuticefficacy in premenopausal patients with stage II/III hormone receptor-positive breast cancerwithout CIA.

Lightweight multiple scale-patch dehazing network for real-world hazy image

( Juan Wang ),( Chang Ding ),( Minghu Wu ),( Yuanyuan Liu ),( Guanhai Chen ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.12

Image dehazing is an ill-posed problem which is far from being solved. Traditional image dehazing methods often yield mediocre effects and possess substandard processing speed, while modern deep learning methods perform best only in certain datasets. The haze removal effect when processed by said methods is unsatisfactory, meaning the generalization performance fails to meet the requirements. Concurrently, due to the limited processing speed, most dehazing algorithms cannot be employed in the industry. To alleviate said problems, a light-weight fast dehazing network based on a multiple scale-patch framework (MSP) is proposed in the present paper. Firstly, the multi-scale structure is employed as the backbone network and the multi-patch structure as the supplementary network. Dehazing through a single net-work causes problems, such as loss of object details and color in some image areas, the multi-patch structure was employed for MSP as an information supplement. In the algorithm image processing module, the image is segmented up and down for processed separately. Secondly, MSP generates a clear dehazing effect and significant robustness when targeting real-world homogeneous and nonhomogeneous hazy maps and different datasets. Compared with existing dehazing methods, MSP demonstrated a fast inference speed and the feasibility of real-time processing. The overall size and model parameters of the entire dehazing model are 20.75M and 6.8M, and the processing time for the single image is 0.026s. Experiments on NTIRE 2018 and NTIRE 2020 demonstrate that MSP can achieve superior performance among the state-of-the-art methods, such as PSNR, SSIM, LPIPS, and individual subjective evaluation.