Effect of alkaline electrolyzed water on physicochemical and structural properties of apricot protein isolate

Zhi-hao Li,Bin Zhou,Xiu-ting Li,Shu-gang Li 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.1

In this current study, comparative study betweenthe effect of electrolyzed water and ultrapure water on theextraction of apricot protein was conducted. The resultsrevealed that under the condition of same pH (pH = 9.5),the extraction efficiency of electrolyzed water on apricotprotein was superior to that of ultrapure water. Moreover,apricot protein (EAP) extracted by electrolyzed water displayedpreferable foaming capacity and emulsion stability. The foaming capacity and emulsion stability of EAP were11.17% and 36.33 min, for UAP, only 4.75% and23.88 min, respectively. Meanwhile, compared to UAP,the secondary structure of EAP was more orderly, in whichthe orderly structures of a-helix and b-sheet were 7.5 and60.2%, while the disorderly structures of b-turn and randomcoil were 8.4 and 23.8%. This work provided a novelextraction strategy, which could improve the extractionrate and minimize the destruction of the structure andfunctional properties of apricot protein.

Comparative analysis of type 2 diabetes-associated gut microbiota between Han and Mongolian people

Shu-chun Li,Yao Xiao,Ri-tu Wu,Dan Xie,Huan-hu Zhao,Gang-yi Shen,En-qi Wu 한국미생물학회 2021 The journal of microbiology Vol.59 No.7

Due to the different rates of diabetes in different ethnic groupsand the structural differences in intestinal microbiota, thisstudy evaluated the changes in diabetes-related intestinal microbiotain two ethnic groups. Fifty-six stool samples werecollected from subjects from the Han and Mongolian ethnicgroups in China, including participants without diabetes(non-diabetic, ND) and with type 2 diabetes (T2D). The 16SrDNA gene V3 + V4 area was extracted from microbiota,amplified by PCR, and used to perform high-throughput sequencingand screen differential microbiota associated withethnicity. The results showed that there were 44 T2D-relatedbacterial markers in the Han subjects, of which Flavonifractor,Alistipes, Prevotella, Oscillibacter, Clostridium XlVa,and Lachnospiracea_incertae_sedis were most closely relatedto diabetes. There were 20 T2D-related bacterial markers inthe Mongolian subjects, of which Fastidiosipila and Barnesiellawere most closely related to diabetes. The commonmarkers of T2D bacteria in the two ethnic groups were Papillibacterand Bifidobacterium. There were 17 metabolic pathwayswith significant differences between the ND and T2Dgroups in the Han group, and 29 metabolic pathways in theMongolian group. The glutamatergic metabolic pathway wasthe only common metabolic pathway in two ethnic groups. The composition and function of diabetes-related bacteriawere significantly different among the different ethnic groups,which suggested that the influence of ethnic differences shouldbe fully considered when studying the association betweendiabetes and bacteria. In addition, the common bacterialmarkers found in diabetic patients of different ethnic groupsin this study can be used as potential targets to study the pathogenesisand treatment of diabetes.

Automated bone segmentation from dental CBCT images using patch-based sparse representation and convex optimization : Segmentation of CBCT image

Wang, Li,Chen, Ken Chung,Gao, Yaozong,Shi, Feng,Liao, Shu,Li, Gang,Shen, Steve G. F.,Yan, Jin,Lee, Philip K. M.,Chow, Ben,Liu, Nancy X.,Xia, James J.,Shen, Dinggang Published for the American Association of Physicis 2014 Medical physics Vol.41 No.4

<P>Cone-beam computed tomography (CBCT) is an increasingly utilized imaging modality for the diagnosis and treatment planning of the patients with craniomaxillofacial (CMF) deformities. Accurate segmentation of CBCT image is an essential step to generate three-dimensional (3D) models for the diagnosis and treatment planning of the patients with CMF deformities. However, due to the poor image quality, including very low signal-to-noise ratio and the widespread image artifacts such as noise, beam hardening, and inhomogeneity, it is challenging to segment the CBCT images. In this paper, the authors present a new automatic segmentation method to address these problems.</P>

Effects of irradiation treatment on protein structure and digestion characteristics of seed-watermelon (Citrullus lanatus var.) kernel protein

Zhi-hao Li,Shang Chu,Ping Wang,Sihai Gao,Shu-gang Li,Xiongwei Yu 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.9

This study examined the effects of differentdoses of irradiation treatments on protein structure anddigestion characteristic of seed-watermelon seed kernelprotein. The results showed that, the molecular structure ofseed-watermelon kernel protein was unfolded after theirradiation treatment, the content of b-sheet structure in thesecondary structure was decreased, while the content ofrandom coil structure increased. The average particle sizeof the protein increased, and the hydrophobic group buriedin the b-sheet structure was exposed hence the surfacehydrophobicity increased. Besides, the surface morphologyof seed-watermelon protein changed from smooth and flatto coarse and concave, the specific surface area in contactwith the aqueous medium increased and its solubilityincreased, the distribution of peptides in the digestabecame wider, and the small molecular weight peptidesgradually increased.

Influence of different parameters on nonlinear friction-induced vibration characteristics of water lubricated stern bearings

Lin Chang-Gang,Zou Ming-Song,Zhang Hai-Cheng,Qi Li-Bo,Liu Shu-Xiao 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

To investigate the mechanism of friction-induced vibration and noise of ship water lubricated stern bearings, a two-degree-of-freedom (2-DOF) nonlinear self-excited vibration model is established. The novelty of this work lies in the detailed analysis of influence of different parameters on the stability and nonlinear vibration characteristics of the system, which provides a theoretical basis for the various friction vibration and noise phenomenon and has a very important directive meaning for low noise design of water lubricated stern bearings. The results reveal that the change of any parameter, such as rotating speed of shaft, contact pressure, friction coefficient, system damping and stiffness, has an important influence on the stability and nonlinear response of the system. The vibration amplitudes of the system increase as (a) rotating speed of shaft, contact pressure, and the ratio of static friction coefficient to dynamic friction coefficient increase and (b) the transmission damping between motor and shaft decreases. The frequency spectrum of the system is modulated by the first mode natural frequency, which is continuous multi-harmonics of the first mode natural frequency. The response of the system presents a quasi-periodic motion.

Optimizing Preparation Conditions for Angiotensin-I-Converting Enzyme Inhibitory Peptides Derived from Enzymatic Hydrolysates of Ovalbumin

Qun Huang,Shu-gang Li,Hui Teng,Yong-guo Jin,Mei-hu Ma,Hong-bo Song 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Angiotensin-I-converting enzyme (ACE) inhibitory peptides were prepared from ovalbumin using enzyme hydrolysis with pepsin as an enzyme source. Effects of pH, enzyme dosage, substrate concentration, hydrolysis temperature, and time on the degree of hydrolysis and the ACE inhibition rate were investigated using single factor experiments. Preparation conditions for ACE inhibitory peptides were optimized using a response surface design on the base of single factor experiments. Optimum preparation conditions were a substrate concentration of 5.2 g/100 mL of D.W with a pH value of 2.5, an enzyme dosage of 14,000 U/g, and a hydrolysis time of 250 min at 30℃. The ACE inhibition rate was up to 70.55±1.13% under these conditions.

Circular RNA cFAM210A, degradable by HBx, inhibits HCC tumorigenesis by suppressing YBX1 transactivation

Yu Jian,Li Wen,Hou Guo-jun,Sun Da-peng,Yang Yuan,Yuan Sheng-xian,Dai Zhi-hui,Yin Hao-zan,Sun Shu-han,Huang Gang,Zhou Wei-ping,Yang Fu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Hepatitis B protein x (HBx) has been reported to promote tumorigenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), but the mechanism awaits further investigation. In this study, we found that cFAM210A (a circular RNA derived from the third exon of transcript NM_001098801 of the FAM210A gene; CircBase ID: hsa_circ_0003979) can be silenced by HBx. cFAM210A expression was downregulated and negatively correlated with tumorigenesis in patients with HBV-related HCC. Furthermore, cFAM210A reduced the proliferation, stemness, and tumorigenicity of HCC cells. Mechanistically, HBx increased the N6-methyladenosine (m6A) level of cFAM210A by promoting the expression of RBM15 (an m6A methyltransferase), thus inducing the degradation of cFAM210A via the YTHDF2-HRSP12-RNase P/MRP pathway. cFAM210A bound to YBX1 and inhibited its phosphorylation, suppressing its transactivation function toward MET. These findings suggest the important role of circular RNAs in HBx-induced hepatocarcinogenesis and identify cFAM210A a potential target in the prevention and treatment of HBV-related HCC.

The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins

Hu, Jian-hong,Li, Qing-Wang,Li, Gang,Chen, Xiao-Yu,Hai-Yang, Hai-Yang,Zhang, Shu-Shan,Wang, Li-Qiang Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.4

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been made to evaluate the effect of LDL on boar sperm motility and other characteristics. The experiment was carried out to investigate the effect of LDL on the freezing of boar sperm in 0.25 ml straws. The aim was to evaluate the quality of boar spermatozoa cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Tris-citric acid-glucose (TCG) and Tris-citric acid-fructose (TCF), two basic freezing extenders containing egg yolk. Similarly, acrosome and plasma membrane integrity were also evaluated and compared to semen cryopreserved with TCG and TCF. Analysis of sperm quality after freeze-thaw showed that the motility, acrosome and plasma membrane integrity were improved with LDL in the extender, as compared to the TCG and TCF. The highest post-thaw integrity of acrosome and plasma membrane and motility were obtained with 9% LDL (w/v). Consequently, the optimum LDL concentration in the extender was 9%. It is also suggested that the concentration of LDL addition is important for the effect on boar sperm protection during freezing and thawing. The percentage of motile spermatozoa was significantly higher after freezing in 9% LDL than in TCG and TCF 54.4% versus 30.4% and 30.1% (p<0.05), respectively. The integrity of acrosome and plasma membrane were also significantly higher at 70.3% and 50.5% respectively with semen frozen in 9% LDL extender compared to TCG at 37.8% and 30.3% and TCF at 36.4% and 29.9%, respectively (p<0.05),. In conclusion, we propose that extender containing LDL extracted from hen egg yolk could be used as a cryoprotective media with a better efficiency than TCG and TCF. LDL improved boar semen quality, allowing better spermatozoa motility, acrosome and plasma membrane integrity after the freeze-thaw process. Furthermore, we found out that the extender with 9% LDL concentration significantly enhanced motility, acrosome and plasma membrane integrity of boar sperm after freezing and thawing.

Molecular characterization of novel haplotypes of eIF4E family in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Shuan-Tao Liu,Zhi-Gang Zhang,Qiao-Yun Li,Shu-Fen Wang,Zhi-Zhong Zhao,Jin-Dong Lu,Wen-Ling Xu,Xian-Xian Liu,Wei-Min Fu 한국유전학회 2013 Genes & Genomics Vol.35 No.6

Two genes coding for eukaryotic translation initiation factors, eIF4E.a and eIF4E.c, were isolated from twelve accessions of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Polymorphism analysis revealed that 94and 142 polymorphic sites were characterized from allele of BraeIF4E.a and BraeIF4E.c which produced complex haplotype structures. Six novel haplotypes were characterized from the two alleles respectively. Among the six novel haplotypes of BraeIF4E.a, three loss-of-function mutations were identified in which a conserved single nucleotide deletion mutation cause the early termination of BraeIF4E.a coding product; while for six new BraeIF4E.c haplotypes, their coding product show amino acid substitution mutations on non-conservative amino acid residues which might affect TuMV infection in Chinese cabbage.

Experimental Study and Numerical Simulation of Precast Shear Wall with Rabbet-Unbonded Horizontal Connection

Chong-fang Sun,Shu-ting Liang,Xiao-jun Zhu,Hu Li,Jian-min Guo,Gang Li,Ya-min Song,Dong-yue Wu 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.1

This paper reports the results of a seismic performance study of a precast shear wall with a new horizontal connection. The new connection is the rabbet-unbonded horizontal connection, which is composed of rabbets and unbonded rebar segments. The rabbets are used to improve the shear capacity and prevent slippage of the connection, and the unbonded rebar segments are used to improve the ductility and energy dissipation. Three specimens were tested with different parameters under cyclic quasi-static loading. The test results showed that the specimen with a larger unbonded level had a richer hysteresis curve, larger ductility, larger energy dissipation, and slightly smaller bearing capacity. Moreover, in relation to the stiffness degradation, in the initial stage, the specimen with a larger unbonded level had a smaller stiffness, whereas in the last stage, the stiffnesses were similar regardless of the unbonded level. A parameter analysis using a finite element model proved that the ductility and energy dissipation of a shear wall with the rabbet-unbonded horizontal connection increased with the unbonded length and level. In addition, when the axial compression ratio increased, the bearing capacity increased, but the load-displacement curves decreased more rapidly. It was concluded that the unbonded length and unbonded level could effectively improve the ductility and energy dissipation of a shear wall. However, they should not be too large under high pressure, and the design suggestions for the new connection need further research considering other factors.