Pseudolaric Acid B Inhibits Proliferation, Invasion and Epithelial-to-Mesenchymal Transition in Human Pancreatic Cancer Cell

Xiaoyu Li,Xianzhi Zhao,Wen Song,Zibin Tian,Lin Yang,Qinghui Niu,Qi Zhang,Man Xie,Bin Zhou,Yonghong Xu,Jun Wu,Cuiping Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1

Purpose: This study was aimed to investigate the effect of pseudolaric acid B (PAB) on proliferation, invasion and epithelial-to-mesenchymal transition (EMT) in pancreatic cancer cells and to explore the possible mechanism. Materials and Methods: The pancreatic cancer cell line SW1990 was cultured and treated with PAB dose- and time-dependent manners. Cell proliferation and invasion ability were measured by MTT assay and Matrigel/Transwell test, respectively. Semi-quantitative real-time polymerase chain reaction and Western blotting were conducted to detect the expression of EMT markers and the key molecules. Finally, nude mice subcutaneous transplantation tumor model was used to confirm the therapy efficacy of PAB. Results: PAB could inhibit SW1990 cell proliferation and invasion in time- and dose-dependent manners. Vimentin, fibronectin, N-cadherin, Snail, Slug, YAP, TEAD1, and Survivin were down-regulated (p<0.01), while E-cadherin, caspase-9, MST1, and pYAP were up-regulated (p<0.05). Combined PAB and gemcitabine treatment markedly restricted the tumor growth compared with gencitabin or PAB alone groups. Conclusion: PAB could inhibit the proliferation and invasion ability of pancreatic cancer cells through activating Hippo-YAP pathway and inhibiting the process of EMT.

Research on risk management in the coal-fired power generation enterprise

Dai Qinghui,Li Sha 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

The paper analyzes the risks which may emerge in the generate electricity enterprise in the process of reforming, such as the system policy risk, coal supply risk, production operation risk, environmental protection risk, administrative supervision risk, electric power price risk,market power risk and credit risk, etc. The fuzzy comprehensive evaluation model of the coal-fired power generation enterprise is set up in this paper. Then, the paper puts forward certain of risk-evading measure, in combination with the characteristics of luck camp of the coal-fired power generation enterprise.

Procedure for Ranking Ground Motion Records Based on the Destructive Capacity Parameter

Jinjun Hu,Qinghui Lai,Shuang Li,Lili Xie 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.1

The relative input energy of a ground motion is used to quantitatively estimate the destructive capacity of ground motion to a structure, which reflects the deformation capacity and energy dissipation capacity of a structure. The destructive capacity of a ground motion on a structure is known to be relevant to the structural period. Because ranking the destructive capacity of ground motion at all periods is not practical, rationally dividing period ranges should be performed before ranking ground motions according to their destructive capacity and a new method to divide period ranges based on the relative input energy is proposed. According to the correlation coefficient of the relative input energy at adjacent structural periods, the structural periods from 0.2 s to 10 s are divided into several representational period ranges, which include a recommended period within each period range to represent the whole range. Only the ground motion ranking sequence at the recommended period is used in this period range. The rationality of the ranking sequence of ground motions based on destructive capacity is verified by a series of structural seismic response analyses. The results of this paper are compared with those of existing methods, and the results show that the relative input energy index is able to better quantitatively describe the destructive capacity of ground motion.

Comparison of Six Automated Immunoassays With Isotope-Diluted Liquid Chromatography–Tandem Mass Spectrometry for Total Thyroxine Measurement

Songlin Yu,Weiyan Zhou,Xinqi Cheng,Qinghui Meng,Honglei Li,Li’an Hou,Jun Lu,Shaowei Xie,Qian Cheng,Chuanbao Zhang,Ling Qiu 대한진단검사의학회 2019 Annals of Laboratory Medicine Vol.39 No.4

Background: Accurate serum total thyroxine (TT4) measurement is important for thyroid disorder diagnosis and management. We compared the performance of six automated immunoassays with that of isotope-diluted liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) as the reference method. We also evaluated the correlation of thyroid stimulating hormone (TSH) with TT4 measured by ID-LC-MS/MS and immunoassays. Methods: Serum was collected from 156 patients between October 2015 and January 2016. TT4 was measured by immunoassays from Abbott (Architect), Siemens (ADVIA Centaur XP), Roche (E601), Beckman-Coulter (Dxi800), Autobio (Autolumo A2000), and Mindray (CL-1000i), and by ID-LC-MS/MS. Results were analyzed using Passing–Bablok regression and Bland–Altman plots. Minimum requirements based on biological variation were as follows: a mean bias of ≤4.5% and total imprecision (CV) of ≤3.7%. Results: All immunoassays showed a correlation >0.945 with ID-LC-MS/MS; however, the slope of the Passing–Bablok regression line varied from 0.886 (Mindray) to 1.23 (Siemens) and the intercept from -12.8 (Siemens) to 4.61 (Mindray). Only Autobio, Beckman-Coulter, and Roche included the value of one in the 95% confidence interval for slope. The mean bias ranged from -10.8% (Abbott) to 9.0% (Siemens), with the lowest value noted for Roche (3.5%) and the highest for Abbott (-10.8%). Only Abbott and Roche showed within-run and total CV ≤3.7%. Conclusions: Though all immunoassays correlated strongly with ID-LC-MS/MS, most did not meet the minimum clinical requirement. Laboratories and immunoassay manufacturers must be aware of these limitations.

Influence of Pore Structure Characteristics on the Mechanical and Durability Behavior of Pervious Concrete Material Based on Image Analysis

Ruyan Liu,Yin Chi,Shengyun Chen,Qinghui Jiang,Xiaoyu Meng,Kun Wu,Shengjia Li 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.4

Pervious concrete has been widely used in parking lots and other lightweight streets. Performances of pervious concrete are strongly dependent on its pore structure characteristics. This paper investigates the relationship among porosity, permeability, compressive strength, durability and the pore structure characteristics of pervious concrete. The influences of basalt fiber and fine aggregate on the pervious concrete are also discussed. Pervious concrete specimens with different pore structures were prepared by combining basalt aggregate with size of 5-10 mm and 10-15 mm at different proportion. The pore structure characteristics such as area porosity, pore sizes, and pore distribution of pervious concrete were obtained and analyzed using image analysis method. Experimental results showed that the pore structure characteristics of pervious concrete have effects on its mechanical and durability behaviors. Homogeneous pore distribution and large distances between pores increase the compressive strength and improve freeze-thaw durability of pervious concrete. Due to the difference in the internal pore structure of pervious concretes, specimens with similar volumetric porosities had different permeability coefficients. A series of numerical models were regenerated through a MATLAB code using pore structural parameters derived from the image analysis method, then the numerical models were fed to ANSYS for meshing and further simulation. The comparison of the simulation and experimental results showed close accuracy, which verified the applied simulation analysis method based on image analysis technology.

Overexpression of a Camellia sinensis DREB transcription factor gene (CsDREB) increases salt and drought tolerance in transgenic Arabidopsis thaliana

Mingle Wang,Jing Zhuang,Zhongwei Zou,Qinghui Li,Huahong Xin,Xinghui Li 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

Dehydration-responsive element-binding protein(DREB) transcription factors play key roles in plant stresssignal transduction pathways. We herein describe the functionsof a Camellia sinensis DREB transcription factor (CsDREB)in response to abiotic stress. Subcellular localization analysesindicated that the CsDREB localizes to the nucleus. CsDREBexpression in C. sinensis leaves was induced by heat, cold,drought, high salinity, H2O2, and exogenous abscisic acid(ABA). Additionally, CsDREB showed no transcriptionalactivation in Saccharomyces cerevisiae. Transgenic Arabidopsisthaliana plants overexpressing CsDREB exhibited enhancedtolerance to salt and drought stresses. The overexpression ofCsDREB in A. thaliana plants resulted in the up-regulatedexpression of ABA-dependent stress-induced genes (i.e.,AtRD29B, AtRAB18, AtABI1, and AtABI2) and ABAindependentstress-induced genes (i.e., AtCOR15a andAtRD29A). Furthermore, an analysis of the CsDREB promotersequence revealed the presence of several abiotic and bioticstress-related motifs, along with the developmental stageandtissue-specific elements. An examination of thetransient expression of the CsDREB promoter in Nicotianabenthamiana leaves revealed that the promoter is highlyresponsive to ABA and methyl jasmonate. Collectively, theseresults suggest that CsDREB may increase plant tolerance tosalt and drought stresses via both ABA-dependent and ABAindependentpathways.