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RISS 인기검색어
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- 저자 : Mingle Wang (검색결과 3 건)
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Mingle Wang,Jing Zhuang,Zhongwei Zou,Qinghui Li,Huahong Xin,Xinghui Li 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Dehydration-responsive element-binding protein(DREB) transcription factors play key roles in plant stresssignal transduction pathways. We herein describe the functionsof a Camellia sinensis DREB transcription factor (CsDREB)in response to abiotic stress. Subcellular localization analysesindicated that the CsDREB localizes to the nucleus. CsDREBexpression in C. sinensis leaves was induced by heat, cold,drought, high salinity, H2O2, and exogenous abscisic acid(ABA). Additionally, CsDREB showed no transcriptionalactivation in Saccharomyces cerevisiae. Transgenic Arabidopsisthaliana plants overexpressing CsDREB exhibited enhancedtolerance to salt and drought stresses. The overexpression ofCsDREB in A. thaliana plants resulted in the up-regulatedexpression of ABA-dependent stress-induced genes (i.e.,AtRD29B, AtRAB18, AtABI1, and AtABI2) and ABAindependentstress-induced genes (i.e., AtCOR15a andAtRD29A). Furthermore, an analysis of the CsDREB promotersequence revealed the presence of several abiotic and bioticstress-related motifs, along with the developmental stageandtissue-specific elements. An examination of thetransient expression of the CsDREB promoter in Nicotianabenthamiana leaves revealed that the promoter is highlyresponsive to ABA and methyl jasmonate. Collectively, theseresults suggest that CsDREB may increase plant tolerance tosalt and drought stresses via both ABA-dependent and ABAindependentpathways.
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Yin, Binfeng,Wang, Yu,Dong, Mingling,Wu, Jing,Ran, Bei,Xie, Mengxia,Joo, Sang Woo,Chen, Yiping Elsevier 2016 Biosensors & bioelectronics Vol.86 No.-
<P><B>Abstract</B></P> <P>A rapid and multiplexed immunosensor was developed based on a quantum dot (QD)-reverse assaying strategy (RAS) and immuno-magnetic beads (IMBs) for one-step and simultaneous detection of <I>Escherichia coli O157: H7</I> and <I>Salmonella</I>. In a conventional QD-based immunosensor, the fluorescence signal of the “IMBs-target-QD” immunoconjugate is directly used as the assaying readout. However, the fluorescence signal is affected by IMBs due to light scattering and the “IMBs-target-QD” immunoconjugate needs multiple washing and re-suspension steps. To address these problems, we use the surplus QD-antibody conjugate as signal readout in the RAS, which prevents interference from the IMBs, increases the fluorescence signal, and avoids complex operations. Compared with conventional QD-based immunosensor, the sensitivity of QD-RSA immunosensor for detection of <I>Escherichia coli O157: H7</I> has been improved fifty-fold, and whole analysis procedure can be finished within 1h. Therefore, this RSA strategy is promising for improving the performance of QD-based immunosensors and could greatly broaden their applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The immunosensor based on quantum dot-reverse assaying strategy (QD-RAS) was developed. </LI> <LI> The sensitivity of QD-RAS improved 50 times compared to conventional QDs-immunosensor. </LI> <LI> The whole analysis in QD-RAS can be finished in one-step within 1h. </LI> </UL> </P>
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Liu, Shanqin,Cai, Mingle,Deng, Renhua,Wang, Jianying,Liang, Ruijing,Zhu, Jintao 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.1
A facile and versatile route to prepare porous polymer microparticles with tunable pore size and density through the combination of phase separation and emulsion-solvent evaporation method is demonstrated. When volatile organic solvent (e.g., chloroform) diffuses through the aqueous phase containing poly(vinyl alcohol) (PVA) and evaporates, n-hexadecane (HD) and polystyrene (PS) in oil-in-water emulsion droplets occur to phase separate due to the incompatibility between PS and HD, ultimately yielding microparticles with porous structures. Interestingly, density of the pores (pore number) on the shell of microparticles can be tailored from one to hundreds by simply varying the HD concentration and/or the rate of solvent evaporation. Moreover, this versatile approach for preparing porous microparticles with tunable pore size and density can be applied to other types of hydrophobic polymers, organic solvents, and alkanes, which will find potential applications in the fields of pharmaceutical, catalyst carrier, separation, and diagnostics.
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