Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

A New Furofuran Lignan from Geranium thunbergii Sieb. et Zuccc.

Liu, Qing-He,Jeong, Ji-Eun,Choi, Eun-Jin,Moon, Young-Hee,Woo, Eun-Rhan The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.12

A new furofuran lignan, 4-hydroxykobusin (3), together with known lignans, kobusin (1), and 7,7'-dihydroxybursherenin (2), were isolated from the whole plant of Geranium thunbergii Sieb. et Zucc (Geraniaceae). The structures were determined based on the spectral data and a comparison with the published data. This is the first report of the presence of furofuran lignan in Geranium species.

SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells

Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.

A New Furofuran Lignan from Geranium thunbergii Sieb. et Zucc.

Qing-He Liu,정지은,Eun Jin Choi,Young Hee Moon,Eun-Rhan Woo 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.12

A new furofuran lignan, 4-hydroxykobusin (3), together with known lignans, kobusin (1), and 7,7'-dihydroxybursherenin (2), were isolated from the whole plant of Geranium thunbergii Sieb. et Zucc (Geraniaceae). The structures were determined based on the spectral data and a comparison with the published data. This is the first report of the presence of furofuran lignan in Geranium species

Inhibitory Activity of IL-6 Production by Flavonoids and Phenolic Compounds from Geranium thunbergii

Qing-He Liu,우은란 한국생약학회 2008 Natural Product Sciences Vol.14 No.1

Three flavonoids (1 - 3) and three phenolic compounds (4 - 6) were isolated from the whole plant of Geranium thunbergii Sieb. et Zucc (Geraniaceae). Their structures were determined by chemical and spectral analysis. These compounds were examined for the inhibitory activity of IL-6 production in TNF-α stimulated MG-63 cell. Among the isolated compounds, gallic acid (4) and gallic acid methyl ester (6) showed potent inhibitory activity.

Association of Single Nucleotide Polymorphisms in the Prostaglandin-endoperoxide Synthase 2 (PTGS2) and Phospholipase A₂ Group IIA (PLA2G2A) Genes with Susceptibility to Esophageal Squamous Cell Carcinoma

Liu, Fen,Wei, Wen-Qiang,Cormier, Robert T.,Zhang, Shu-Tian,Qiao, You-Lin,Li, Xin-Qing,Zhu, Sheng-Tao,Zhai, Yan-Chun,Peng, Xiao-Xia,Yan, Yu-Xiang,Wu, Li-Juan,He, Dian,He, Yan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background: The prostaglandin-endoperoxide synthase 2 (PTGS2) and phospholipase A2 group IIA (PLA2G2A) genes encode enzymes that are involved in arachidonic acid and prostaglandin biosynthesis. Dysregulation of both genes is associated with inflammation and carcinogenesis, including esophageal squamous cell carcinoma (ESCC). We therefore hypothesized that there is an association between single nucleotide polymorphisms (SNPs) in these genes and susceptibility to ESCC. Methods: We performed a gene-wide tag SNP-based association study to examine the association of SNPs in PTGS2 and PLA2G2A with ESCC in 269 patients and 269 healthy controls from Taihangshan Mountain, Henan and Hebei Provinces, the rural area of China which has the highest incidence of esophageal cancer in the world. Thirteen tag SNPs in PLA2G2A and 4 functional SNPs in PTGS2 were selected and genotyped using a high-throughput Mass Array genotyping platform. Results: We found a modest increased risk of ESCC in subjects with the PTGS2 rs12042763 AA genotype (OR=1.23; 95% CI, 1.00-3.04) compared with genotype GG. For PLA2G2A, a decreased risk of ESCC was observed in subjects with the rs11677 CT (OR=0.51, 95%CI, 0.29-0.85) or TT genotype (OR=0.51, 95%CI, 0.17-0.96) or the T carriers (CT+TT) (OR=0.52, 95%CI, 0.31-0.85) when compared with the CC genotype. Also for PLA2G2A, rs2236771 C allele carriers were more frequent in the control group (P=0.02). Subjects with the GC (OR=0.55, 95%CI, 0.33-0.93) or CC genotype (OR=0.38, 95% CI, 0.16-0.94) or the C carriers (GC+CC) (OR=0.52, 95%CI, 0.32-0.85) showed a negative association with ESCC susceptibility. Conclusions: Our results suggest that PTGS2 and PLA2G2A gene polymorphisms may modify the risk of ESCC development.

Nanosheets of copolymerized ionic liquid-based polyelectrolyte complexes regulated at oil–water interface and their emulsification capability

He Zhang,Jun Bo Zhang,Qing Bo Meng,Wei Guo,Ming Yang,Shuyao Wu,Qiong Wu,Daliang Liu,Xi-Ming Song 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

The emulsification by polyelectrolyte complexes is easy to handle, and could be considered as an effectivestrategy for introducing functional materials to liquid–liquid interfaces, but the related studies are stillinsufficient. Herein, a kind of copolymerized ionic liquid (PIL-co-PVIm) which is synthesized from 1-vinyl-3-ethylimidazolium bromide and 1-vinylimidazole, are used as polycation to form polyelectrolytecomplexes type Pickering emulsifiers with sodium alginate, poly(sodium 4-styrenesulfonate) and poly(acrylic acid) sodium salt respectively as polyanion. For toluene-water emulsion systems andhydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm]PF6)-watersystem, the polyelectrolyte complexes formed by PIL-co-PVIm and sodium alginate or poly (sodium4-styrenesulfonate) showed best emulsification capability. The emulsions stabilized by the polyelectro-lyte complexes at stoichiometric ratio are relatively less stable compared to non-stoichiometriccomplexes, while PIL-co-PVIm without complexation has no emulsification capability. The polyelectro-lyte complexes based on homopolymerized PIL give an unsatisfied emulsification capability, while theemulsification performance could be remarkably improved by copolymerizing ionic liquids with theuncharged comonomer, but excessive contents of the uncharged unit make the polyelectrolytecomplexes less interface active. The sheet-like PECs are formed in aqueous dispersions, and theirthickness is remarkably reduced at oil–water interface after emulsification, approximately ranging from200 nm to 10 nm by manipulating the polyanion.

Percutaneous Sacroplasty for Painful Sacral Metastases Involving Multiple Sacral Vertebral Bodies: Initial Experience with an Interpedicular Approach

Qing-Hua Tian,He-Fei Liu,Tao Wang,Ying-Sheng Cheng,Chun-Gen Wu 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.6

Objective: To report our initial experience of percutaneous sacroplasty (PSP) with an interpedicular approach for treating painful sacral metastases involving multiple sacral vertebral bodies. Materials and Methods: This study prospectively enrolled 10 consecutive patients (six men and four women; mean age, 56.3 ± 13.8 years) who underwent PSP for painful sacral metastases involving multiple sacral vertebral bodies from March 2017 to September 2018. Visual analogue scale (VAS) scores, Oswestry disability index (ODI) values, and the number of opioids prescribed to the patients were assessed before and after PSP. The procedure duration, length of hospitalization, and complications were also recorded. Results: Mean VAS and ODI declined significantly from 6.90 ± 1.20 and 74.40 ± 5.48 before the procedure to 2.70 ± 1.34 and 29.60 ± 14.57 after the procedure, respectively (p < 0.01). The median number of opioids prescribed per patient decreased from 2 (interquartile range [IQR] 1–3) pre-procedure to 1 (IQR 0–3) post-procedure (p < 0.01). Nine of the 10 patients showed no or decreased opioid usage, and only 1 patient showed unchanged usage. The mean procedure duration was 48.5 ± 3.0 minutes. The average length of hospitalization was 4.7 ± 1.7 days. Extraosseous cement leakage occurred in three cases without causing any clinical complications. Conclusion: PSP with an interpedicular approach is a safe and effective treatment in patients with painful sacral metastases involving multiple sacral vertebral bodies and can relieve pain and improve mobility.

Dietary Factors and Risk of Pancreatic Cancer: a Multi-Centre Case-Control Study in China

Liu, Shu-Zheng,Chen, Wan-Qing,Wang, Ning,Yin, Meng-Meng,Sun, Xi-Bin,He, Yu-Tong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: Pancreatic cancer is the sixth leading cause of cancer death with an increasing trend in China. Dietary intake is believed to play an important role in pancreatic cancer carcinogenesis. The aim of this paper was to evaluate associations between some dietary factors and risk of pancreatic cancer in a multi-centre case-control study conducted in China. Materials and Methods: Cases (n=323) were ascertained from four provincial cancer hospitals. Controls (n=323) were randomly selected from the family members of patients without pancreatic cancer in the same hospitals, 1:1 matched to cases by gender, age and study center. Data were collected with a questionnaire by personal interview. Odds ratios (OR) and 95% confidence intervals (95%CI) were estimated using conditional logistic regression. Results: Tea intake (OR =0.49; 95%CI: 0.30-0.80) was associated with a half reduction in risk of pancreatic cancer. Reduced vegetable consumption (P trend: 0.04) was significant related to pancreatic cancer. Although no significant association was found for meat and fruit, ORs were all above or below the reference group. A protective effect was found for fruit (OR=1.73 for consumption of 1-2 times/week vs more than 3 times/week; 95%CI: 1.05-2.86). A high intake of meat was associated to a higher risk of pancreatic cancer (OR=0.59 for consumption of 1-2 times/week vs. more than 3 times/week; 95%CI: 0.35-0.97). Conclusions: The present study supports fruit consumption to reduce pancreatic cancer risk and indicates that high consumption of meat is related to an elevated risk. Direct inverse relations with tea and vegetable intake were also confirmed.

Assessment of g‒Mg3N2 membrane performance for efficient removal of dioxane contaminant from wastewater: DFT‒MD simulation treatments

Liu Huazhong,He Wenxuan,Xu Han,Wang Xiaolan,An Qing 한국물리학회 2023 Current Applied Physics Vol.54 No.-

We implemented DFT calculations for gas phase and DFT‒MD simulations for ambient conditions to evaluate the efficient performance of g‒Mg3N2 monolayer in 1,4‒dioxane removal from wastewater. The geometries, interaction energies, and interaction nature of the adsorbed dioxane and H2O molecules on the Mg3N2 surface were investigated. It was found that the interaction of H2O exceeds that of dioxane by twelve though, the two adsorbates follow rather a similar trend in the adsorption process. The AIM analysis was carried out to investigate the nature of bonding for adsorbed dioxane/H2O on the Mg3N2 surface. Furthermore, DFT‒MD simulation shows that dioxane tends to adsorb on the Mg3N2 nanosheet in the presence of water molecules at ambient conditions, making the Mg3N2 potentially suitable adsorbent for dioxane adsorption in aqueous solution. This exploration presents a foundation for the capability of computational study for predicting the adsorption and remediation of organic contaminants from wastewater.