유기합성 및 천연물 검색을 통한 의약품 개발의 최근 동향

우은란 전남대학교 약품개발연구소 1999 약품개발연구지 Vol.8 No.1

모과나무 줄기의 화학성분

우은란,신지은,김홍광,김청룡 한국생약학회 2011 생약학회지 Vol.42 No.3

Chaenomeles sinensis Koehne (Rosaceae) is a deciduous tree and is distributed in China, Korea and Japan. In previous studies on the fruits of C. sinensis, some triterpenoid compouds such as oleanolic acid, tormentic acid were reported. In an ongoing investigation into biologically active compounds from natural products, the methanol extract of the stems of C. sinensis was investigated. By means of the repeated column chromatography using silica gel, Sephadex LH-20, LiChroprep RP-18, betulin (1), tormentic acid (2), 1-β-D-glucopyranosyl-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-α-L-rhamnopyranoside (4) were isolated. The chemical structures of compounds 1-4 were determined by the basis of physico-chemical properties and spectroscopic methods such as 1D and 2D NMR. For the isolated compounds (1-4), the inhibitory activity of IL-6production in TNF-α stimulated MG-63 cell was examined. Among the isolates, betulin (1), 1-β-D-glucopyranosyl-3,4,5-trimethoxybenzene (3), lyoniresinol-2a-O-α-L-rhamnopyranoside (4) showed inhibitory effects on IL-6 production in TNF-αstimulated MG-63 cell.

수종의 생약이 과화산화수소에 의한 Hypoxanthine-guanine Phosphoribosyl Transferase (HPRT) 유전자 돌연변이 억제에 미치는 효과

유호진,우은란 한국생약학회 2004 생약학회지 Vol.35 No.1

DNA damage induced by reactive oxygen species (ROS) seems to play an important role in the induction of muta-tion and cancer. Hydrogen peroxide (H2O2) has been shown to induce a variety of genetic alterations, probably by the generationof hydroxyl radicals via Fenton reaction. In this study, we examined the ability of medicinal herbs in the suppression of H2O2-induced mutagenesis. Human fibroblast GM00637 cells were treated with H2O2 in the presence or absence of medicinal herbs,and H2O2-induced mutant frequency was measured at the hypoxanthine guanine phosphoribosyltransferase (HPRT) locus.Treatment of cells with various doses of H2O2 caused a significant increase of the HPRT mutant frequency. However, pre-treatment of cells with several medicinal herbs reduced H2O2-induced mutant frequency. The strong antimutagenic effects wereobserved from the methylene chloride and ethyl acetate fractions of Selaginella tamariscina, Panax ginseng, and Angelica acuti-loba; ethyl acetate fractions of Rehmania glutinosa, Leonurus sibiricus, Curcuma zedoaria and Commiphora molmol; butanolfractions of Scutellaria barbata, Tribulus terrestris, Curcuma zedoaria, Cyperus rotundus and Carthamus tinctorius, whichwere more than 60% inhibition of H2O2-induced mutant frequency at the HPRT locus.

능이 자실체의 생리활성

문영희,우은란,박영준 한국생약학회 2005 생약학회지 Vol.36 No.4

Sarcodon aspratus (Thelepholaceae), a native mushroom, is distributed in Korea and Japan, and has been widely used in traditional food and folk medicines. To confirm the biological activities of Sarcodon aspratus, the liver protecting activity, anti-clotting activity, and anti-complementary activity of the water extract, EtOH extract, and the water soluble proteoglycan part of S. aspratus were investigated. The EtOH, and water extract of S. aspratus decreased the GOT and GPT releases induced by CCl4 in a dose-dependent manner. On the other hand, the water soluble proteoglycan part of S. aspratus showed weak inhibitory activity. In addition, the EtOH and water extract of S. aspratus prevented CCl4-induced hepatotoxicity, as described by a liver histopathologic study. To confirm the anti-clotting activity, the APTT and PT assay were carried out. As a result, only the crude proteoglycan part of S. aspratus showed the anti-coagulating activity, and this result might be due to the inhibition of intrinsic clotting system. Also, the crude proteoglycan part of S. aspratus showed the anti-complementary activity, and the IC50 value was 50 µl/ml.

Sarcodon aspratus에서 분리한 수용성 단백다당체의 항암효과

문영희,우은란,박영준 한국생약학회 2002 생약학회지 Vol.33 No.3

자귀나무 뿌리껍질의 식물화학적 성분연구

고재종,우은란,문영희 한국생약학회 2004 생약학회지 Vol.35 No.3

Repeated column chromatography of an ethyl acetate extract of the root bark of Albizzia julibrissin Durazz afforded four compounds, euscaphic acid ester glucoside (1), luteolin-7-O-neohesperidoside (2), (+)-medioresinol (3), (-)-syringaresinol (4). Their structures were determined by chemical and spectroscopic methods. Compounds 1-4 were isolated from this plant for the first time. Among these compounds, (+)-medioresinol (3) exhibited moderate cytotoxic activity against XF 498 and HCT 15 cell line.

한련초로부터 정제한 다당체의 혈당강하 효과

문영희,우은란,이동행 한국생약학회 2003 생약학회지 Vol.34 No.3

팔손이나무(Fatsia japonica) 줄기의 성분연구

이 환,우은란,이동성 한국생약학회 2021 생약학회지 Vol.52 No.4

Fatsia japonica is grown wild to Eastern Asia, including Korea, Japan, and Taiwan and it is known as ornamental plant, and it is also known that pharmacological action. In this study, we have selected the stem of F. japonica with consideration about biological activities and amount of yield. In addition, four compounds (1-4) were isolated from the stem of F. japonica. Extensive spectroscopic and chemical studies established the structures of these compounds as maltose (1), begoniifolide A (2), leiyemudanoside B (3), leonticin F (4). All of the compounds were investigated for their anti-inflammatory, anti-neuroinflammatory, and neuro-protective effects on RAW264.7, BV2, and HT22 cells. However, among four compounds, there were no effects by maltose (1), begoniifolide A (2), leiyemudanoside B (3), leonticin F (4) on the anti-inflammatory, anti-neuroinflammatory, and neuro-protective action. This is the first report on the isolation of maltose (1), begoniifolide A (2), leiyemudanoside B (3), leonticin F (4) from the stem of F. japonica. Begoniifolide A (2), leiyemudanoside B (3), leonticin F (4) were isolated for the first time from this plant. It might be necessary to continue the further studies to find the biological active compounds isolated from the stem of F. japonica.

Studies on the Antibacterial Activity and Active Constituents of Sonbanghwalmyoungum

이범용,안덕균,우은란,박호군,Lee, Beom-Yong,Ahn, Duk-Kyun,Woo, Eun-Rhan,Park, Ho-Koon The Society of Korean Medicine 1998 대한한의학회지 Vol.19 No.1

약효(藥效) 및 안정성(安定性)이 입증(立證)된 한약(韓藥)으로부터 새로운 항균제(抗菌劑)를 도출(導出)하고자, 임상(臨床)에서 청열작용(淸熱作用)이 있거나 염증성(炎症性) 질환(疾患)과 박테리아의 감염(感染)으로 비롯된 질환(疾患)에 자주 쓰이는 22개(個)의 처방(處方)에 대(對)해 paper disk 법(法)을 사용(使用)하여 항균(抗菌) 효능(效能)을 검색(檢索)하였다. 시료(試料)는 한약(韓藥)의 고용(股用) 방법(方法)을 고려(考慮)하여 물로 추출(抽出)하였으며, 사용(使用) 균주(菌柱)로는 그람 양성균(陽性菌)인 포도상구균(葡萄狀球菌)과 고초균(枯草菌) 그리고 그람 음성균(陰性菌)인 대장균(大腸菌)과 녹농균(綠膿菌)을 사용(使用)하였고, 대조물질(對照物質)로는 tetracycline을 사용(使用)하여 상법(常法)에 의(依)해 실험(實驗)하였다. 22개(個)의 처방(處方) 가운데 백두옹탕(白頭翁湯), 황연해독탕(黃連解毒湯), 증황연황연해독탕(增黃連黃連解毒湯), 사심탕(瀉心湯), 용담사간탕(龍膽瀉肝湯), 선방활명음(仙方活命飮) 등(等)의 처방(處方)이 탁월(卓越)한 항균(抗菌) 활성(活性)을 보였다. 이 가운데 본(本) 연구(硏究)에서는 탕제(湯劑)의 구성(構成) 약재(藥材)가 다른 처방(處方)들에 비(比)해 비교적(比較的) 다양(多樣)한 선방활명음(仙方活命飮)에 대(對)해 활성(活性) 성분(成分)의 규명(糾明) 및 구성(構成) 약재간(藥材間)의 상화작용(相互作用)을 밝히고자 하였다. 선방활명음(仙方活命飮) 5 kg을 메탄올 (MeOH)로 추출(抽出)한 뒤 우수(優秀)한 항균(抗菌) 활성(活性)을 보인 에칠아세테이트 (EtOAc) 분획(分劃)에 대(對)하여 silica gel 및 Sephadex LH-20 column chromatography를 반복(反復)하여 stilbene 계열(系列)의 화합물(化合物)인 Rhapontigenin (Compound 1)과 Rhaponticin (Compound 2)을 얻었다. S. aureus SG 511에 대(對)해 Rhapontigenin은 50 mg/ml 이상(以上) 농도(濃度)에서 항균(抗菌) 효과(效果)를 나타내었고, Rhaponticin은 $200\;mg/m{\ell}$ 농도(濃度)에서 미약(微弱)하게 유효(有效)한 항균(抗菌) 효과(效果)를 나타내었으며, B. subtilis ATCC 6633에 대(對)해 Rhapontigenin은 50 mg/ml 이상(以上) 농도(濃度)에서 항균(抗菌) 효과(效果)를 나타내었고, Rhaponticin은 200 mg/ml 농도(濃度)에서 우수(優秀)한 항균(抗菌) 효과(效果)를 나타내었으며, 100 mg/ml 농도(濃度)에서 미약(微弱)하게 유효(有效)한 항균(抗菌) 효과(效果)를 나타내었다. 또한 E. coli 055에 대(對)해서는 Rhapontigenin과 Rhaponticin 모두 200 mg/ml 농도(濃度)에서 미약(微弱)하게 유효(有效)한 항균(抗菌) 효과(效果)를 나타내었다. 이상(以上)의 결과(結果)로 보아 선방활명음(仙方活命飮)의 항균(抗菌) 효능(效能)은 군약(君藥)인 대황(大黃)의 성분(成分) 중(中)의 하나인 stilbene 계열(系列)의 화합물(化合物)인 Rhapontigenin과 Rhaponticin의 작용(作用)에 의(依)한 것이며, 이는 한의학(韓醫學) 방제(方劑) 원리(原理)인 군신좌사(君臣佐使) 이론(理論)에서 군약(君藥)이 주증(主症)에 주(主)로 작용(作用)하는 약물(藥物)이라는 것을 밝혀주는 것이라고 사료(思料)된다.

Anti-Candida Property of a Lignan Glycoside Derived from Styrax japonica S. et Z. via Membrane-Active Mechanisms

Cana Park,우은란,이동건 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.6

Styraxjaponoside C was investigated with respect to its antifungal activity and mechanisms of action. Devoid of hemolytic activity, Styraxjaponoside C demonstrated an antifungal effect against the human pathogenic yeast Can-dida albicans in an energy-independent manner. To char-acterize the mechanisms of the antifungal activity of Styraxjaponoside C, fluorescence analysis with membrane probe 1,6-diphenyl-1,3,5-hexatriene, and flow cytometric analysis on C. albicans were conducted. The results showed that Styraxjaponosdie C induced cytoplasmic membrane perturbation. The current study suggested that Styraxjaponoside C was active against C. albicans with membrane-active mechanisms.