Fabrication and Characterization of Vertically Aligned ZnO Nanorod Arrays via Inverted Monolayer Colloidal Crystals Mask

Cheng Chen,Taotao Ding,Zhiqiang Qi,Wei Zhang,Jun Zhang,Juan Xu,Jingwen Chen,Jiangnan Dai,Changqing Chen 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.4

The periodically ordered ZnO nanorod (NR) arrays have been successfully synthesized via a hydrothermal approach on thesilicon substrates by templating of the TiO2ring deriving from the polystyrene (PS) nanosphere monolayer colloidal crystals(MCC). With the inverted MCC mask, sol–gel-derived ZnO seeds could serve as the periodic nucleation positions for thesite-specific growth of ZnO NRs. The large-scale patterned arrays of single ZnO NR with good side-orientation can be readilyproduced. According to the experimental results, the as-integrated ZnO NR arrays showed an excellent crystal quality andoptical property, very suitable for optoelectronic applications such as stimulated emitters and ZnO photonic crystal devices.

Effect of Vanadium on the Microstructure and Mechanical Properties of 2100 MPa Ultra-High Strength High Plasticity Spring Steel Processed by a Novel Online Rapid-Induction Heat Treatment

Qi‑Lei Dai,Kun Li,Kai‑Ren Meng,Zhou Fang,Wen Chen,Tian‑Bao Yang,Chi Feng,Jin‑Ming Wu,R. D. K. Misra 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.4

Advanced automotive industries generate large demand for the next generation of high strength and high toughness springsteels. Vanadium-containing 55SiCrV spring steels subjected to rapid-induction heating treatment can fulfil such requirements. However, the effect of vanadium microalloying under online rapid-induction heat treatments is rarely reported. Acomparative study of the microstructure and tensile properties of 55SiCr and 55SiCrV spring steel wires subjected to a novelonline rapid induction heat treatment has been demonstrated herein. It is found that the tensile strength of the 55SiCr springwire decreases with the decrease in the wire speed in online rapid-induction heating, and the plasticity increases. Whereas,the tensile strength of the 55SiCrV steel wire increases with the decrease in the wire speed with the retained high plasticity,which is attributed to the strengthening effect of the dislocations. Through the optimized rapid-induction heating/coolingthermal cycles and intermediate-temperature tempering treatment, the tensile strength of the 55SiCrV steel wire approaches2106 MPa with total elongation of 9.7%. Compared with the 55SiCr spring steel, the addition of V in 55SiCrV spring steelchanges the strengthening and toughening mechanisms via the grain refinement and enhancement in the hardenability andtempering resistance. The finely dispersed V-containing secondary phases are rarely found in the matrix, which indicates thatthe precipitation effect stemming from the addition of V is not the dominant strengthening factor in the online rapid-inductionheat process. The proposed novel online rapid-induction heat treatment provides a promising pathway for the mechanicalproperty improvement of the spring steel.

The ways for ginsenoside Rh2 to fight against cancer: the molecular evidences in vitro and in vivo.

Qi-rui Hu,Yao Pan,Han-cheng Wu,Zhen-zhen Dai,Qing-xin Huang,Ting Luo,Jing Li,Ze-yuan Deng,Fang Chen The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

Cancer is a global public health issue that becomes the second primary cause of death globally. Considering the side effects of radio- or chemo-therapy, natural phytochemicals are promising alternatives for therapeutic interventions to alleviate the side effects and complications. Ginsenoside Rh2 (GRh2) is the main phytochemical extracted from Panax ginseng C.A. Meyer with anticancer activity. GRh2 could induce apoptosis and autophagy of cancer cells and inhibit proliferation, metastasis, invasion, and angiogenesis in vitro and in vivo. In addition, GRh2 could be used as an adjuvant to chemotherapeutics to enhance the anticancer effect and reverse the adverse effects. Here we summarized the understanding of the molecular mechanisms underlying the anticancer effects of GRh2 and proposed future directions to promote the development and application of GRh2.

Evaluate Dry Deposition Velocity of the Nitrogen Oxides Using Noah-MP Physics Ensemble Simulations for the Dinghushan Forest, Southern China

Qi Zhang,Ming Chang,Shengzhen Zhou,Weihua Chen,Xuemei Wang,Wenhui Liao,Jianing Dai,ZhiYong Wu 한국기상학회 2017 Asia-Pacific Journal of Atmospheric Sciences Vol.53 No.4

There has been a rapid growth of reactive nitrogen (Nr) deposition over the world in the past decades. The Pearl River Delta region is one of the areas with high loading of nitrogen deposition. But there are still large uncertainties in the study of dry deposition because of its complex processes of physical chemistry and vegetation physiology. At present, the forest canopy parameterization scheme used in WRF-Chem model is a single-layer “big leaf” model, and the simulation of radiation transmission and energy balance in forest canopy is not detailed and accurate. Noah-MP land surface model (Noah-MP) is based on the Noah land surface model (Noah LSM) and has multiple parametric options to simulate the energy, momentum, and material interactions of the vegetation-soil-atmosphere system. Therefore, to investigate the improvement of the simulation results of WRF-Chem on the nitrogen deposition in forest area after coupled with Noah-MP model and to reduce the influence of meteorological simulation biases on the dry deposition velocity simulation, a dry deposition single-point model coupled by Noah- MP and the WRF-Chem dry deposition module (WDDM) was used to simulate the deposition velocity (Vd). The model was driven by the micro-meteorological observation of the Dinghushan Forest Ecosystem Location Station. And a series of numerical experiments were carried out to identify the key processes influencing the calculation of dry deposition velocity, and the effects of various surface physical and plant physiological processes on dry deposition were discussed. The model captured the observed Vd well, but still underestimated the Vd. The self-defect of Wesely scheme applied by WDDM, and the inaccuracy of built-in parameters in WDDM and input data for Noah-MP (e.g. LAI) were the key factors that cause the underestimation of Vd. Therefore, future work is needed to improve model mechanisms and parameterization.

Robust Design of Coordinated Set Planning with the Non-Ideal Channel

( Jianxin Dai ),( Shuai Liu ),( Ming Chen ),( Jun Zhou ),( Jie Qi ),( Jingwei Liang ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.5

In practical wireless systems, the erroneous channel state information (CSI) sometimes deteriorates the performance drastically. This paper focuses on robust design of coordinated set planning of coordinated multi-point (CoMP) transmission, with respect to the feedback delay and link error. The non-ideal channel models involving various uncertainty conditions are given. After defining a penalty factor, the robust net ergodic capacity optimization problem is derived, whose variables to be optimized are the number of coordinated base stations (BSs) and the divided area`s radius. By the maximum minimum criterion, upper and lower bounds of the robust capacity are investigated. A practical scheme is proposed to determine the optimal number of cooperative BSs. The simulation results indicate that the robust design based on maxmin principle is better than other precoding schemes. The gap between two bounds gets smaller as transmission power increases. Besides, as the large scale fading is higher or the channel is less reliable, the number of the cooperated BSs shall be greater.

CpG Array Analysis of Histone H3 Lysine 4 Trimethylation by Chromatin Immunoprecipitation Linked to Microarrays Analysis in Peripheral Blood Mononuclear Cells of IgA Nephropathy Patients

Suwen Qi,Yong Dai,Weiguo Sui,Ming Yang,Jiejing Chen 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.2

Purpose: The purpose of the present study was to investigate the aberrance of histone H3 lysine 4 trimethylation (H3K4me3) in patients with IgA Nephropathy (IgAN). Materials and Methods: In this study, H3K4me3 variations in peripheral blood mononuclear cells (PBMCs) from 15 IgAN patients and 15 healthy subjects were analyzed using chromatin immunoprecipitation linked to microarrays analysis (ChIP-chip). ChIP real-time PCR was used to validate the microarray results. Expression analysis by quantitative real-time PCR (qRT-PCR) revealed correlations between mRNA and H3K4me3 levels. DNA methylation status was analyzed by quantitative methylation-specific PCR. Results: We found that 321 probes displayed significant H3K4me3 differences in IgAN patients compared with healthy controls. Among these probes, 154 probes displayed increased H3K4me3and 167 probes demonstrated decreased H3K4me3. For further validation, we selected 4 key relevant genes (FCRL4, GALK2, PTPRN2 and IL1RAPL1) to study. The results of ChIP real-time PCR coincided well with the microarray data. Quantitative RT-PCR revealed the correlations between the mRNA expression and the methylation levels of H3K4me3. Different degrees of DNA methylation alterations appeared on the selected positive genes. Conclusion: Our studies indicated that there were significant alterations in H3K4me3 in IgAN patients. These findings may help to explain the disturbed immunity and abnormal glycosylation involved in IgAN patients.

Structural Characteristics of SiGe/Si Materials Investigated by Raman Spectroscopy

Chang Chun Chen,Ben Hai Yu,Jiang Feng Liu,Qi Run Dai 대한금속학회 2005 METALS AND MATERIALS International Vol.11 No.4

A High-Throughput Method Based on Microculture Technology for Screening of High-Yield Strains of Tylosin-Producing Streptomyces fradiae

Yao Zhiming,Fan Jingyan,Dai Jun,Yu Chen,Zeng Han,Li Qingzhi,Hu Wei,Yan Chaoyue,Hao Meilin,Li Haotian,Li Shuo,Liu Jie,Huang Qi,Li Lu,Zhou Rui 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6

Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by highperformance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 μg/ml) and UN-C137 (6889.72 ± 70.25 μg/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 μg/ml). These mutant strains will form the basis for further strain breeding in tylosin production.

Response of Glucocorticoid Receptor Alpha and Histone Deacetylase 2 to Glucocorticoid Treatment Predicts the Prognosis of Sudden Sensorineural Hearing Loss

Xiuling Zhang,Jinxiang Chen,Ziwen Gao,Hui Qi,Yanhong Dai,Wandong She 대한이비인후과학회 2019 Clinical and Experimental Otorhinolaryngology Vol.12 No.4

Objectives. To investigate glucocorticoid receptor (GR) and histone deacetylase 2 (HDAC2) gene expression and protein levels in peripheral blood mononuclear cells (PBMCs) of patients with severe or profound sudden sensorineural hearing loss (SSNHL) and to explore the roles of GRs and HDAC2 in glucocorticoid (GC) insensitivity. Methods. Fifty-five severe or profound SSNHL patients were enrolled in the study. According to hearing improvement after GC treatment, patients were assigned into two groups: GC-sensitive and GC-resistant. A normal reference group included 20 healthy volunteers without hearing loss. Quantitative real-time polymerase chain reaction and Western blot analyses were used to detect the relative expression of GRα, GRβ, and HDAC2 in PBMCs at the mRNA and protein levels. Results. The protein levels of GRs and HDAC2 in PBMCs of SSNHL patients were lower than the normal reference values before GC treatment. Compared with the GC-resistant group, both the mRNA and protein levels of GRα and HDAC2 were significantly increased in the GC-sensitive group after GC treatment. Conclusion. A lack of GRα and HDAC2 induction following steroid treatment in GC-resistant SSNHL patients may play a fundamental mechanistic role in GC insensitivity. Response of GRα and HDAC2 to steroid treatment may, thus, predict the prognosis of hearing improvement in SSNHL patients.

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.