Possible Epithelial Ovarian Cancer Association with HPV18 or HPV33 Infection

Zhang, Pei-Pei,Zhou, Lei,Cao, Jia-Shi,Li, Yi-Ping,Zeng, Zhi,Sun, Ni,Shen, Li,Zhu, Hao-Yue,Ruan, Yang,Zha, Wen-Ting,Wang, Xin-Yu,Zhang, Ke-Qiang,Zhang, Ran Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.6

The present study was conducted to investigate the prevalence of HPV infection in epithelial ovarian cancer (EOC) in Hunan province. DNA samples were collected from paraffin embedded ovarian tissue from 322 patients with EOC, 99 with ovarian benign tumors and 199 normal persons. The polymerase chain reaction and direct sequencing were used to identify the HPV types in the samples. The relationship between the infection of human papillomavirus (HPV) and the epithelial ovarian carcinoma (EOC) was investigated combined with clinical data. The prevalence of HPV18 and HPV33 in EOC group and benign group was higher than in the normal group. HPV18 and HPV33 may play a role in the development of both EOC and ovarian benign tumor and may participate in the development of EOC with traditional risk factors, family history and abortion, possibly exerting synergistic effects.

Network Intrusion Detection Based on Directed Acyclic Graph and Belief Rule Base

Bang-Cheng Zhang,Guan-Yu Hu,Zhi-Jie Zhou,You-Min Zhang,Pei-Li Qiao,Lei-Lei Chang 한국전자통신연구원 2017 ETRI Journal Vol.39 No.4

Intrusion detection is very important for network situation awareness. While a few methods have been proposed to detect network intrusion, they cannot directly and effectively utilize semi-quantitative information consisting of expert knowledge and quantitative data. Hence, this paper proposes a new detection model based on a directed acyclic graph (DAG) and a belief rule base (BRB). In the proposed model, called DAG-BRB, the DAG is employed to construct a multi-layered BRB model that can avoid explosion of combinations of rule number because of a large number of types of intrusion. To obtain the optimal parameters of the DAG-BRB model, an improved constraint covariance matrix adaption evolution strategy (CMA-ES) is developed that can effectively solve the constraint problem in the BRB. A case study was used to test the efficiency of the proposed DAG-BRB. The results showed that compared with other detection models, the DAG-BRB model has a higher detection rate and can be used in real networks.

Seismic Behaviors of Steel Bar Reinforced Joints of Concrete Filled Steel Tubular Laminated Columns

Yingying Zhang,Yuan Huang,Ke Lei,Jianing Pei,Qilin Zhang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.9

This paper presents the numerical studies on seismic behaviors of steel bar reinforced joints of Concrete Filled Steel Tubular Laminated Columns (CFSTLC). First, the material constitutive relations and modeling details are introduced. Then, the failure mode, bearing capacity and energy dissipation of CFSTLC joints under cyclic loading are studied, in which the effects of concrete grade, steel grade and reinforcement arrangement are discussed. Finally, the restoring force model is proposed to describe the seismic behaviors of bar reinforced joints. Results show that the bar reinforced joints perform good seismic performance. There are three main failure modes, including flexural failure of beam, shear failure of column and mixed failure. Increasing concrete grade and reinforcement ratio of column can increase the ultimate bearing capacity and energy dissipation capacity. Using bundled longitudinal reinforcement bars in beams can slightly reduce the ultimate bearing capacity, but can improve the energy dissipation capacity of specimens with flexural failure modes. Increasing the reinforcement ratio and steel grade can decrease the degradation rate of structural stiffness. The tri-linear restoring force model can make a good prediction of hysteretic behaviors of CFSTLC joints.

Seismic behaviors of ring beams joints of steel tube-reinforced concrete column structure

Yingying Zhang,Jianing Pei,Yuan Huang,Ke Lei,Jie Song,Qilin Zhang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.27 No.4

This paper presents the seismic behaviors and restoring force model of ring beam joints of steel tube-reinforced concrete column structure under cyclic loading. First, the main failure mode, ultimate bearing capacity, stiffness degradation and energy dissipation capacity are studied. Then, the effects of concrete grade, steel grade, reinforcement ratio and radius-to-width ratios are discussed. Finally, the restoring force model is proposed. Results show that the ring beam joints of steel tube-reinforced concrete column structure performs good seismic performances. With concrete grade increasing, the ultimate bearing capacity and energy dissipation capacity increase, while the stiffness degradation rates increases slightly. When the radius-width ratio is 2, with reinforcement ratio increasing, the ultimate bearing capacity decreases. However, when the radius-to-width ratios are 3, with reinforcement ratio increasing, the ultimate bearing capacity increases. With radius-to-width ratios increasing, the ultimate bearing capacity decreases slightly and the stiffness degradation rate increases, but the energy dissipation capacity increases slightly.

Probability Evaluation of Excess Voltage in a Distribution Network with Uneven Charging Electric Vehicle Load

Chen Wei,Zhang Lei,Pei Xiping 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.1

Traditional deterministic power fl ow analysis does not take into account the randomness of the charging load due to electric vehicles. In order to accurately assess the impact of charging load on the voltage quality of a distribution network, we present a method of evaluating the probability of excessive voltage in a distribution network with an uneven charging electric vehicle load, based on Latin hypercube sampling. First, we establish a probability model of uneven charging load of an electric vehicle, and construct a sample matrix for the uneven charging scenario using Latin hypercube sampling. The dynamic probability distribution of the voltage across the nodes is obtained using a probabilistic power fl ow calculation and non-parametric kernel density estimation to realize a probability assessment of the voltage quality of the distribution network. Finally, a simulation analysis using an IEEE 30-node system shows that the proposed method is accurate and eff ective

Surface Parameter Measurement of Braided Composite Preform Based on Faster R-CNN

Zhitao Xiao,Lei Pei,Lei Geng,Ying Sun,Fang Zhang,Jun Wu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

Pitch length and surface braiding angle are two important parameters of braided composite preforms. In this paper,a method based on Faster R-CNN is proposed to measure the two parameters. First, after image acquisition, a fabric imagedatabase including initial cropped images, augmented images, and target images is established. Then, the target images areclassified into four categories according to the gray change characteristics. Third, a Faster R-CNN fabric detection model istrained on the fabric image database. Fourth, targets are detected by the trained network, and corners are detected based onthe detected targets. Finally, pitch lengths and surface braiding angles are measured based on the detected corners. Experimental results show that the proposed method achieves the automatic measurement of pitch lengths and surfacebraiding angles of 2D and 3D braided composite preforms with high accuracy.

Protective association of Klotho rs495392 gene polymorphism against hepatic steatosis in non-alcoholic fatty liver disease patients

Wen-Yue Liu,Xiaofang Zhang,Gang Li,Liang-Jie Tang,Pei-Wu Zhu,Rafael S. Rios,Kenneth I. Zheng,Hong-Lei Ma,Xiao-Dong Wang,Qiuwei Pan,Robert J. de Knegt,Luca Valenti,Mohsen Ghanbari,Ming-Hua Zheng 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.2

Background/Aims: Non-alcoholic fatty liver disease (NAFLD) is closely associated with metabolic dysfunction. Among the multiple factors, genetic variation acts as important modifiers. Klotho, an enzyme encoded by the klotho (KL) gene in human, has been implicated in the pathogenesis of metabolic dysfunctions. However, the impact of variants in KL on NAFLD risk remains poorly understood. The aim of this study was to investigate the impact of KL rs495392 C>A polymorphism on the histological severity of NAFLD. Methods: We evaluated the impact of the KL rs495392 polymorphism on liver histology in 531 Chinese with NAFLD and replicated that in the population-based Rotterdam Study cohort. The interactions between the rs495392, vitamin D, and patatin-like phospholipase domain containing 3 (PNPLA3) rs738409 polymorphism were also analyzed. Results: Carriage of the rs495392 A allele had a protective effect on steatosis severity (odds ratio [OR], 0.61; 95% confidence interval [CI], 0.42–0.89; P=0.010) in Chinese patients. After adjustment for potential confounders, the A allele remained significant with a protective effect (OR, 0.66; 95% CI, 0.45–0.98; P=0.040). The effect on hepatic steatosis was confirmed in the Rotterdam Study cohort. Additional analysis showed the association between serum vitamin D levels and NAFLD specifically in rs495392 A allele carriers, but not in non-carriers. Moreover, we found that the rs495392 A allele attenuated the detrimental impact of PNPLA3 rs738409 G allele on the risk of severe hepatic steatosis. Conclusions: The KL rs495392 polymorphism has a protective effect against hepatic steatosis in patients with NAFLD.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Using SiNx double-layer deposition to reduce electrode incidence of short circuits due to impurity particles in thin film transistor preparation

Zhao Shuo,Yu Xiang,Pei Jingxuan,Yang Lei,Zhang Zhiqiang,Zhang Pingping 한국물리학회 2023 Current Applied Physics Vol.48 No.-

Aviation safety relies on accurate scope presentation of air navigation displays, and lack of display information due to poor dot line may cause the pilot to misjudge the air conditions. Impurity particles occurred during preparing thin film transistor (TFT) circuit may be a leading reason for poor dot line. This work attempts to replace existing SiNx single-layer deposition with double-layer, so that the incidence risk can be lower through reducing number and size of the impurity particles. Double-layer deposition approach has the dot line structures and performances to that of single-layer, and can meet the requirements of display usage. Results of field test demonstrate that double-layer deposition reduces defect rate of display more efficient than single-layer. Proposed mechanism of double-layer deposition illuminates that 2nd-SiNx can cover holes generated by falling impurity particles to avoid short circuits in both indium tin oxide (ITO) and source-drain (SD) contact.

Novel Phage Display-Derived H5N1-Specific scFvs with Potential Use in Rapid Avian Flu Diagnosis

( Jie Wu ),( Xian Qiao Zeng ),( Hong Bin Zhang ),( Han Zhong Ni ),( Lei Pei ),( Li Rong Zou ),( Li Jun Liang ),( Xin Zhang ),( Jin Yan Lin ),( Chang Wen Ke ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5

The highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtype infect poultry and have also been spreading to humans. Although new antiviral drugs and vaccinations can be effective, rapid detection would be more efficient to control the outbreak of infections. In this study, a phage-display library was applied to select antibody fragments for HPAI strain A/Hubei/1/2010. As a result, three clones were selected and sequenced. A hemagglutinin inhibition assay of the three scFvs revealed that none exhibited hemagglutination inhibition activity towards the H5N1 virus, yet they showed a higher binding affinity for several HPAI H5N1 strains compared with other influenza viruses. An ELISA confirmed that the HA protein was the target of the scFvs, and the results of a protein structure simulation showed that all the selected scFvs bound to the HA2 subunit of the HA protein. In conclusion, the three selected scFVs could be useful for developing a specific detection tool for the surveillance of HPAI epidemic strains.