Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model

Lei, Xuelian,Cai, Lin,Li, Xiao,Xu, Hua,Geng, Chong,Wang, Chunhui The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.3

$Na^+/H^+$ exchangers (NHEs) have been shown to be involved in regulating cell volume and maintaining fluid and electrolyte homeostasis. Pooled evidences have suggested that loss of $Na^+/H^+$ exchanger isoform 8 (NHE8) impairs intestinal mucosa. Whether NHE8 participates in the pathology of infectious colitis is still unknown. Our previous study demonstrated that somatostatin (SST) could stimulate the expression of intestinal NHE8 so as to facilitate $Na^+$ absorption under normal condition. This study further explored whether NHE8 participates in the pathological processes of infectious colitis and the effects of SST on intestinal NHE8 expression in the setting of infectious colitis. Our data showed that NHE8 expression was reduced in Citrobacter rodentium (CR) infected mice. Up-regulation of NHE8 improved diarrhea symptom and mucosal damage induced by CR. In vitro, a similar observation was also seen in Enteropathogenic E. coli (EPEC) infected Caco-2 cells. Seglitide, a SST receptor (SSTR) 2 agonist, partly reversed the inhibiting action of EPEC on NHE8 expression, but SSTR5 agonist (L-817,818) had no effect on the expression of NHE8. Moreover, SST blocked the phosphorylation of p38 in EPEC-infected Caco-2 cells. Taken together, these results suggest that enhancement of intestinal NHE8 expression by SST could ameliorate the symptoms of mice with infectious colitis.

Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy

Xing Lei,Li Yanqin,Li Wenhao,Liu Rong,Geng Yuanming,Ma Weiqun,Qiao Yu,Li Jianwen,Lv Yingtao,Fang Ying,Xu Pingping 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). METHODS: The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesisrelated indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14 days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. RESULTS: Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p\0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p\0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. CONCLUSION: It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.

Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model

Xuelian Lei,Lin Cai,Xiao Li,Hua Xu,Chong Geng,Chunhui Wang 대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.3

Na+/H+ exchangers (NHEs) have been shown to be involved in regulating cell volume and maintaining fluid and electrolyte homeostasis. Pooled evidences have suggested that loss of Na+/H+ exchanger isoform 8 (NHE8) impairs intestinal mucosa. Whether NHE8 participates in the pathology of infectious colitis is still unknown. Our previous study demonstrated that somatostatin (SST) could stimulate the expression of intestinal NHE8 so as to facilitate Na+ absorption under normal condition. This study further explored whether NHE8 participates in the pathological processes of infectious colitis and the effects of SST on intestinal NHE8 expression in the setting of infectious colitis. Our data showed that NHE8 expression was reduced in Citrobacter rodentium (CR) infected mice. Up-regulation of NHE8 improved diarrhea symptom and mucosal damage induced by CR. In vitro, a similar observation was also seen in Enteropathogenic E. coli (EPEC) infected Caco-2 cells. Seglitide, a SST receptor (SSTR) 2 agonist, partly reversed the inhibiting action of EPEC on NHE8 expression, but SSTR5 agonist (L-817,818) had no effect on the expression of NHE8. Moreover, SST blocked the phosphorylation of p38 in EPEC-infected Caco-2 cells. Taken together, these results suggest that enhancement of intestinal NHE8 expression by SST could ameliorate the symptoms of mice with infectious colitis.

Decomposition of Interference Hyperspectral Images Based on Split Bregman Iteration

( Jia Wen ),( Lei Geng ),( Cailing Wang ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.7

Images acquired by Large Aperture Static Imaging Spectrometer (LASIS) exhibit obvious interference stripes, which are vertical and stationary due to the special imaging principle of interference hyperspectral image (IHI) data. As the special characteristics above will seriously affect the intrinsic structure and sparsity of IHI, decomposition of IHI has drawn considerable attentions of many scientists and lots of efforts have been made. Although some decomposition methods for interference hyperspectral data have been proposed to solve the above problem of interference stripes, too many times of iteration are necessary to get an optimal solution, which will severely affect the efficiency of application. A novel algorithm for decomposition of interference hyperspectral images based on split Bregman iteration is proposed in this paper, compared with other decomposition methods, numerical experiments have proved that the proposed method will be much more efficient and can reduce the times of iteration significantly.

Water-solid Suspension Grafting of Dual Monomers on Polypropylene to Prepare Ion-imprinted Fibers for Selective Adsorption of Cr(VI)

Zhengwei Luo,Lei Li,Mulin Guo,Hui Jiang,Wenhua Geng,Wuji Wei,Zhouyang Lian 한국섬유공학회 2020 Fibers and polymers Vol.21 No.12

In this work, a new strategy was developed to prepare Cr(VI)-imprinted polypropylene (PP) fibers-based materialsfor the adsorption of Cr(VI) from water. Granular PP was modified with dual monomers (glycidyl methacrylate (GMA) andacrylamide (AM)) by water-solid suspension grafting copolymerization. Then, this fibers morphology was obtained by meltblowing. Hence, the preparation steps of the fibrous Cr(VI)-imprinted adsorbent involved surface ion-imprinting andcrosslinking. The influence of the grafting conditions on the grafting percentage was investigated. The maxima graftingpercentages of GMA and AM were 16.4 and 10.6 %, respectively. The chemically modified products showed good mobilityand stability, thus meeting the requirements of melt-blowing method. The prepared Cr(VI)-imprinted polymers showed amaximum adsorption capacity to Cr(VI) of 43.2 mg/g at pH 3. The adsorption data were well fitted by the pseudo-secondorderand Langmuir adsorption models. The adsorbent showed good selectively toward Cr(VI) in a mixed solution containingCu(II) and SO42−. The X-ray photoelectron spectroscopy demonstrated that the adsorbed Cr(VI) was partly reduced to Cr(III).

An Algorithm for Data Aggregation Scheduling with Long-lifetime and Low-latency in Wireless Sensor Networks

Zhengyu Chen,Geng Yang,Lei Chen,Jin Wang 보안공학연구지원센터 2012 International Journal of Future Generation Communi Vol.5 No.4

Surface Parameter Measurement of Braided Composite Preform Based on Faster R-CNN

Zhitao Xiao,Lei Pei,Lei Geng,Ying Sun,Fang Zhang,Jun Wu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

Pitch length and surface braiding angle are two important parameters of braided composite preforms. In this paper,a method based on Faster R-CNN is proposed to measure the two parameters. First, after image acquisition, a fabric imagedatabase including initial cropped images, augmented images, and target images is established. Then, the target images areclassified into four categories according to the gray change characteristics. Third, a Faster R-CNN fabric detection model istrained on the fabric image database. Fourth, targets are detected by the trained network, and corners are detected based onthe detected targets. Finally, pitch lengths and surface braiding angles are measured based on the detected corners. Experimental results show that the proposed method achieves the automatic measurement of pitch lengths and surfacebraiding angles of 2D and 3D braided composite preforms with high accuracy.

Polymorphisms in the uncoupling protein 3 gene and their associations with feed efficiency in chickens

Jin, Sihua,Yang, Lei,He, Tingting,Fan, Xinfeng,Wang, Yiqiu,Ge, Kai,Geng, Zhaoyu Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.9

Objective: The uncoupling protein 3 (UCP3) is a member of the mitochondrial anion carrier superfamily and has crucial effects on growth and feed efficiency in many species. Therefore, the objective of the present study was to examine the association of polymorphisms in the UCP3 gene with feed efficiency in meat-type chickens. Methods: Six single nucleotide polymorphisms (SNPs) of the UCP3 gene were chosen to be genotyped using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in meat-type chicken populations with 724 birds in total. Body weight at 49 (BW49) and 70 days of age (BW70) and feed intake (FI) in the interval were collected, then body weight gain (BWG) and feed conversion ratio (FCR) were calculated individually. Results: One SNP with a low minor allele frequency (<1%) was removed by quality control and data filtering. The results showed that rs13997809 of UCP3 was significantly associated with BWG and FCR (p<0.05), and that rs13997811 had significant effects on BW70 and BWG (p<0.05). Rs13997812 of UCP3 was strongly associated with BW70, FI, and FCR (p<0.05). Furthermore, individuals with AA genotype of rs13997809 had significantly higher BWG and lower FCR (p<0.05) than those with AT genotype. The GG individuals showed strongly higher BW70 and BWG than AA birds in rs13997811 (p<0.05). Birds with the TT genotype of rs13997812 had significantly greater BW70 and lower FCR compared with the CT birds (p<0.05). In addition, the TAC haplotype based on rs13997809, rs13997811, and rs13997812 showed significant effects on BW70, FI, and FCR (p<0.05). Conclusion: Our results therefore demonstrate important roles for UCP3 polymorphisms in growth and feed efficiency that might be used in meat-type chicken breeding programs.

Trends in Specific Immunotherapy for Allergic Rhinitis: A Survey of Chinese ENT Specialists

Han Zhou,Qi-Lei Tao,Jun-Min Wei,Geng Xu,Lei Cheng 대한천식알레르기학회 2014 Allergy, Asthma & Immunology Research Vol.6 No.4

Purpose: Specific immunotherapy (SIT) is a suitable but uncommon treatment option for allergic rhinitis (AR) in China. The current understandingand attitude of Chinese ENT (ear, nose, and throat) specialists in regards to SIT is unclear. This study investigates current trends in the awarenessand application status of SIT among Chinese ENT specialists. Methods: We performed a nationwide, cross-sectional survey with a specially designedquestionnaire given to 800 ENT specialists in China. A member of the trained research group conducted face-to-face interviews with eachrespondent. Results: Most of the respondents considered AR (96.0%) and allergic asthma (96.0%) the most suitable indications for SIT. Of all respondents,77.0% recommended the application of SIT as early as possible; in addition, SIT was considered ‘relatively controllable and safe’ bymost respondents (80.6%). The highest allergen-positive rate in AR was associated with house dust mite (47.7%) and obvious differences existedamong geographical regions. Conventional subcutaneous immunotherapy was the most highly recommended treatment option (96.2%). ‘The highcost of SIT’ (86.6%) and ‘lack of patient knowledge of SIT’ (85.2%) were probably the main reasons for the lower clinical use of SIT in China. Conclusions:Most cases showed that the opinions of Chinese ENT specialists appeared to be in agreement with recent SIT progress and internationalguidelines; however, many areas still need to enhance the standardization and use of SIT in China. Clinical guidelines for SIT require improvement;in addition, Chinese ENT specialists need continuing medical education on SIT.

Association of polymorphisms in Pit-1 gene with growth and feed efficiency in meat-type chickens

Sihua Jin,Tingting He,Lei Yang,Yucui Tong,Xingyong Chen,Zhaoyu Geng 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.11

Objective: The pituitary specific transcription factor-1 (Pit-1) gene is responsible for pituitary development and growth hormone expression and is regarded as a pivotal candidate gene for growth and production in chickens. Therefore, the aim of this study was to investigate the association of Pit-1 polymorphisms with growth and feed efficiency traits in yellow meat-type chickens. Methods: In the present study, five single nucleotide polymorphisms (SNPs) of Pit-1 were selected and genotyped by high-throughput matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in 724 meat-type chickens. Results: Association analysis showed that rs13687126 of Pit-1 was strongly associated with body weight gain (BWG) and feed intake (FI) (p<0.05), and that rs13687128 was significantly correlated with body weight at 70 days of age (BW70), BWG and feed conversion ratio (FCR) (p<0.05). SNP rs13905622 was strongly related to BW70 and FCR (p<0.05). Furthermore, birds with the GG genotype of rs13687126 had larger BWG and FI than those with the AG genotype (p<0.05). Individuals with the TT genotype of rs13687128 were significantly higher BW70 and BWG than those of the CT and CC genotype, while FCR was just the opposite (p<0.05). For rs13905622, the AA chickens showed strongly larger BW70 and lower FCR compared with the AT and TT chickens (p<0.05). Additionally, an ACA haplotype based on rs13687126, rs13687128, and rs13905622 had significant effects on BW70 and FCR (p<0.05). Conclusion: Our studies thus provide crucial evidence for the relationship between polymorphisms of Pit-1 and growth and feed efficiency traits which may be useful for meat-type chicken breeding programs.