Streptobacillus sp.가 생산하는 pullulanase에 관한 연구

오두환,지희숙,최형택,신원철,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.2

A bacterium with the highest pullulanase activity was isolated from the soil and identified as Streptobacillus sp. Y-716. The optimum culture condition of Streptobacillus sp. for the production of pullulanase was as follows; 1.2%(w/v)soluble starch, 0.7%(w/v) CH₃COONH₄, 0.05%(w/v) MgSO₄7 H₂O, 0.01%(w/v)FeSO₄7H₂O, at 30℃, for 48 hrs. The pullulanase from the culture broth of Streptobacillus sp. Y-716 was purified about 46 fold by ammonium sulfate precipitation, dialysis. DEAE-cellulose and Sephadex G-200 column chromatography. The purified enzyme was stable between pH 5.0 and pH 7.5. It was inactivated rapidly above 35℃. The Km value and Vmax of the purified enzyme were 0.0137mg/m/ and 0.666μm/min. on pullulan, respectively.

멸치를 이용한 식품가공용 중간소재의 가공

오광수,노락현,이응호,박희열 國立統營水産專門大學 附設 水産科學硏究所 1989 수산과학연구소보고 Vol.1 No.-

멸치를 보다 효율적으로 이용하기 위한 일련의 연구로서 각종 조리재료나 식품가공용 중간소재로 이용할 수 있는 냉동멸치조미육의 가공조건을 구명하고 본 시제품의 화학성분을 분석하였다. 냉동멸치조미육제품을 가공하기 위하여 채육, 세절한 멸치육에 대해 분리대두단백:물:대두유(1:5:2)로 만든 유화커어드 12.8%, 식염 0.5%, 설탕 2.0%, sodium bicarbonate 0.4%, 중합인산염 0.2%, MSG 0.2%, 분리대두단백 3.05, 양파가루 0.3%, 생강가루 0.1%, 후추가루 0.1% 및 항산화제로서 sodium erythrobate를 0.2% 첨가하여 멸치육 알맹이가 붕괴되지 않도록 혼합한후 -35℃에서 급속동결시켜 carton box로 포장하여 -25±2℃에 동결 저장하는 것이 가장 좋았다. 냉동멸치조미육제품의 수분함량은 73.0%, 조단백질 19.1%, 조지방은 5.0%였으며 생균수는 1.5~1.6x 10^5/g, histamine 함량은 70.6mg/100g이었다. 유화커어드를 첨가함으로써 제품의 지방함량 조정 및 조직감을 개선시킬 수 있었다. 제품의 주요구성 지방산은 linoleic, oleic, palmitic, docosahexaenoic, linolenic, palmitoleic, eicosapentaenoic acid 등으로 고도불포화지방산이 많이 함유되어 있었고, Glu, Asp, Leu, Lys, Als 등이 주요 구성아미노산이었다. 멸치조미육제품의 정미성분은 IMP(160.9mg/100g)와 같은 핵산관련물질 및 Glu(179.4mg/100g), His(85.7mg/100g), Ala(54.4mg/100g), Leu(37.9mg/100g) 등 유리아미노산이 정미성 및 약적으로 보아 주체를 이루고 있었고, 이 외에 total creatinine (158.3mg/100g), 미량의 TMAO, betaine 등으로 이루어져 있었다. Processing conditions and food components of frozen seasoned anchovy meat products were investigated. The separated anchovy meat was chopped, mixed with 12.8% emulsion curd, 0.5% table salt, 2.0% sugar, 0.4% sodium bicarbonate, 0.2% polyphosphate, 0.2% monosodium glutamate, 0.3% onion powder, 0.1% garlic powder, 0.1% ginger powder, 3.0% soybean protein, and 0.2% sodium erythorbate by remodeled stone mortar. This seasoned anchovy meat was frozen with contact freezer, packed in a carton box and then stored at -25±2℃. The major fatty acids of product were linoleic, oleic, palmitic, docosahexaenoic, linolenic, palmitoleic, eicosapentaenoic acid. Amino acid composition of product were mainly consisted of Glu, Asp, Leu, Lys and Ala. The taste compounds of product were IMP 160.0 mg/100g; free amino acids such as Glu, His, Ala, Leu 503.7 mg/100g; total creatinine 158.3 mg/100g and small amounts of betaine, TMAO.

Filton과 활성탄에 의한 Emulsified Oil흡착에 관한 연구

오현제,고종희,정연규 연세대학교 산업기술연구소 1987 논문집 Vol.19 No.2

This study is an experimental research to determine the adsorption capacity of emulsified oil of Filton(FA) in comparison with that of Activated Carbon(AC). Batch type study was conducted with varying the following factors; pH, temperature, adsorbent size, and dosage. The results are as follows. 1. Freundlich isotherms of emulsified oil were; q = ?? for FA and q = ?? for AC. 2. Adsorption capacity of FA was 18.4 mg of Oil/g of FA, while that of AC was 12.8mg of Oil/g of AC. 3. Above 50% of FA adsorption was occurred within 30 minutes of contact time, the equilibrium state was reached in three hours of contact time both FA and AC. 4. Removal efficiency of emulsified oil by adsorbents was increased with the smaller size of adsorbents, the larger amount of dosage, the higher temperature, and the lower value of pH.

간호사의 근무의욕과 업무수행 및 소진경험에 관한 연구

오현이,강희영,백영주 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

This study was designed to investigate the relationship among morale, task performance. and burnout experience of nurses. Two hundred and fourteen nurses working in clinical settings were selected by purposing sampling at 1 university hospital and 2 general hospitals in Kwangju. The data was collected from August 31 to September 9, 1992. For this study, three instruments were used. For measuring nurse's morale, Choi's instrument was used. The questionnaire is corn posed of 32 items. For measuring task performance, Lee's Nursing Performance Evaluation Tool was used The questionnaire is composed of 44 items For measuring nurse's burnout experience used which developed by Pines. The questionnaire is composed of 21 items. The data were analyzed by frequency, t-test. ANOVA. Pearson 's Correlation Coefficient. The results of the study were as follows: 1. The level of nurse's morale was 2.90 point(by 5 point scale) The level of nurse's task performance was 3.50 point (by 5 point scale) and the level of nurse's burnout experience was 3.64 point (by 7 point scale) 2. The relationship among morale, task performance and burnout experience of nurses. The 1st hypothesis. "The higher the level of morale, the level of task performance would be higher." was supported(r=0.455. p<0.001). The 2nd hypothesis, "The higher the level of morale, the level of burnout experience would be lower." was supported (r=-0.572, P<0.001). The 3rd hypothesis. "The higher the level of task performance, the level of burnout experience would be lower." was supported(r=-0.280. P<0.001).

Hoffmann반사를 이용한 선택적 척수 후근 절제술을 위한 근전도 기기와 프로그램의 개발 : 척수 신경근 자극시의 근전도 양상에 대한 연구 A Study on the EMG Patterns Generated by Stimulation of the Spinal Nerve Roots

오창완,권성훈,김희찬,왕규창,김현집 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.6

대구지역 거주자의 식생활 습관과 위장질환과의 관계

오미정,김수월,양희면,현재식,허천식,이명화 한국건강관리협회 2000 건협 Report Vol.3 No.-

외과적 악교정술을 위한 두부방사선학 계측 기준치

성재현,성정옥,경희문,권오원 대한치과교정학회 1989 대한치과교정학회지 Vol.19 No.1

The purpose of this study was to establish Korean normas that will be valuable aids for diagnosis in orthognathic surgery patients. Lateral cephalometric measurements were established for adult Koreans (60 males, 62 females) with normal occlusion and acceptable profiles. The results were as follows: 1. Means, standard deviation and sexual differentiation were included within each measurement. 2. In skeletal mesurements, FH to SN and SN to mandibular plane measurements in females were larger than those in males while all linear measurements in males were larger than those in males were larger than those in females. 3. Significant differences were observed between male and female groups on all of the items that showed vertical relationship in dental measurments. And the measurements of ADH, PDH and overbite in males were larger than those in females while the measurements of incisor exposure in females were larger than those in males. 4. In all measurements of soft tissue except NLA, that is, of FCA, UFH, ULL and LLL, males were larger than those in females.

煉炭灰 魚汁吸着飼料 開發에 關한 硏究

鄭鉉丞,李乙熙,金哲旭,宋瑛敏,吳錫斗 진주산업대학교 농업기술연구소 1990 農業技術硏究所報 Vol.3 No.-

우리나라에서 年間 50萬M/T 生産廢水處理되는 魚汁中의 蛋白質, U.G.F., Ca等과 991萬M/T 生産廢棄되는 煉炭灰中의 Fe, Zn等의 微量鑛物質을 混合한 煉炭灰 魚汁吸着飼料(FBF:Fish-soluble-abosorbed briquette ash feed)를 開發하기 爲하여 一次로 1981年부터 1984年까지 煉炭灰의 成分分析과 煉炭灰 添加給與試驗을 哺乳中仔豚과 育成-肥育豚에 實施하였고 哺乳中 仔豚에 實施하고 있는 1, 2次 鐵分注射代用 給與試驗을 거쳐 添加給與飼料化 可能性 確認後 1985年부터 1989年까지 煉炭灰 魚汁吸着飼料를 製造하여 5∼10% 添加 給與試驗結果 優秀한 成績이 나타났으므로 3%, 5%, 10% 水準을 代替配給한 飼料를 製造하여 哺乳仔豚의 鐵分注射代用으로서의 效果, 育成期, 肥育期, 育性-肥育期의 飼養試驗, 試驗飼料의 製造,貯藏性, 消化率 및 經濟性, 豚의 背脂肪層,屠體率, 血液像 等으로 調査한 바 다음과 같다. 1. 煉炭灰와 煉炭의 一般成分 및 鑛物質 分析結果 煉炭灰의 成分含量은 수분(0.15%), 조단백질(0.52%), 조지방(0%), 조섬유(4.13%), NFE(0%), 조회분(95.35%), Ca(0.807%), P(0.074%), K(0.138%), Na(0.025%), Mg(0.183%), Fe(3,769mg/kg), Mn(98mg/kg), Zn(139mg/kg) 및 Cu(21.0mg/kg)이였고 煉炭은 NFE(10.96%), 조회분(71.35%), Ca(0.224%), P(0.043%), K(0.158%), Na(0.039%), Mg(0.191%), Fe(7,385mg/kg), Mn(116mg/kg), Zn(306mg/kg) 및 Cu(17.5mg/kg)이었다. 2. 煉炭灰 魚汁吸着飼料成分 分析 煉炭灰 500g에 濃縮魚汁量을 50g에서 250g 및 1,250g 乾物換算(1:1) 水準까지 混合시킨 煉炭灰 魚汁吸着飼料의 成分含量은 濃縮魚汁量이 50g일때는 各各 11.69% 및 31.31%와 2.61% 및 10.6%로 增加하였고 粗纖維와 粗灰分은 各各 5.51% 및 3.86%와 62.82% 및 50.42%로 감소하였다. 아미노酸 成分에서 lysine의 境遇 濃縮魚汁量이 50g일 때 0.19%로 나타났으나 濃縮魚汁量 增加에 比例하여 250g일 때 0.75%로 나타났다. 기타의 아미노酸들도 濃縮 魚汁量이 增加할수록 아미노산 含量이 높게 나타나는 傾向을 보였다. 鑛物質成分은 Ca, Fe, Zn, Mg, Mn 및 Co등은 濃縮魚汁量인 높을수록 높게 나타났다. 3. 哺乳中의 成長期와 離乳後 育成-肥育期에 微量鑛物質 供給濟로서 煉炭灰를 自由 採食시켰을 때 (1) 增體量은 配合飼料 單飼區보다 配合飼料 + 煉炭灰 給與區가 多少 높았으나 有意性은 認定되지 않았다(p>0.05). (2) 飼料效率은 配合飼料單飼區(4.71)보다 煉炭灰 給與 育成肥育區(4.84)가 약간 낮게 나타났다. (3) 仔豚育成區에서는 飼料效率이 3.0이었으며 萎縮豚에 效果가 좋은 것으로 나타났다. (4) 經濟性 分析結果 1kg增體當 飼料肥로 換算하면 配合飼料單飼區(829원)에서 比해 配合飼料 + 煉炭灰區(852원)가 23원이 增加하였다. (5) 仔豚育成區에서는 1kg 增體當 528원이 소요되었다. (6) 煉炭灰의 嗜好性은 좋은 편이었고 건강상태도 양호했다. 4. 哺乳仔豚에 鐵分注射代用으로서 效果 및 離乳仔豚의 育成期에 微量鑛物質 供給源으로서 煉炭灰를 3, 5, 7% 水準으로 添加 給與하였을 때 (1) 哺乳中 鐵分注射 代用으로 煉炭灰를 自由採食 시켜도 仔豚의 發育成績과 健康狀態는 異狀이 없었고 比較的 良好한 便이었다. (2) 增體量은 煉炭灰를 3% 添加 給與하였을 때 가장 좋게 나타났다(P>0.05). (3) 飼料要求量은 對照區와 處理區間에는 큰 差異가 없었다(p>0.05). (4) 1kg增體에 所要된 飼料費는 T1(3% 첨가구), T2(5% 첨가), C 및 T3(7% 첨가구)에서 636.40, 646.72, 672.52 및 684.56원이었다. (5) 對照區에 比해 處理區에서의 血色素 含量은 암·수 供히 良好하였다. 5. 母豚과 哺乳仔豚에 煉炭灰를 自由 採食시켰을때의 乳汁을 通한 鐵分이 哺乳仔豚에 미치는 影響과 哺乳仔豚이 直接 採食하므로서 哺乳中 實施하는 鐵分注射代用으로서의 效果는 (1) 生後 3週間의 體重은 本 大學(5.27kg)이 原種豚(5.00kg)보다 다소 높게 나타났다. (2) 育成數 및 育成率은 本 大學(8.0頭, 86.0%)이 原種豚(8.3頭, 88.3%)보다 낮게 나타났다. (3) 離乳時 體重은 本 大學(17.8kg)이 原種豚(17.4kg) 보다 多少 높게 나타났다. (4) 分娩時期와 哺乳仔豚數가 같은 區에서 鐵分注射區와 煉炭灰給與區間의 比較成績은 煉炭灰給與區가 多少 좋게 나타났으나 有意差는 없었다(p>0.05). (5) 哺乳中 鐵分注射代用으로 煉炭灰를 自由採食시켜도 仔豚의 發育成績과 健康狀態는 異狀이 없었고 比較的 良好한 便이었다. 5. 哺乳仔豚에 2回 實施하고 있는 鐵分注射를 1回로 줄이고 哺乳母豚과 仔豚에 煉炭灰를 自由 採食시켰을 때 (1) 哺乳中 21日齡의 體重은 鐵分注射 1回 煉炭灰給與區(4.48kg)가 鐵分注射 2回區(4.43kg)보다 苦干 좋게 나타났다. (2) 離乳時體重은 鐵分注射 1回 煉炭灰給與區(16.8kg)가 鐵分注射 2回區(17.4kg) 보다 낮게 나타났다. (3) 15週齡의 體重은 鐵分注射 1回 煉炭灰給與區(48.85kg)가 鐵分注射 2回區(46.0kg) 보다 좋게 나타났다. (4) 終了時 體重은 鐵分注射 1回 煉炭灰給與區(78.3kg)가 鐵分注射 2回區(71.6kg) 보다 좋게 나타났다. (5) 日當 增體量은 鐵分注射 1回 煉炭灰給與區(0.589kg)가 鐵分注射 2回區(0.537kg) 보다 좋게 나타났다. (6) 飼料效率은 鐵分注射 1回 煉炭灰給與區(2.32)가 鐵分注射 2回區(2.34)보다 낮게 나타났다. (7) 鐵分注射 1回 煉炭灰給與區의 健康狀態는 正常이었다. 以上의 結果로 生後 3週齡까지의 鐵分注射 1回代替로서 煉炭灰 給與가 可能하고 育成-肥育 前期까지 微量鑛物質 供給源으로 煉炭灰 給與效果가 있는 것으로 나타났다. 7. 1981年부터 1984年까지 4年間 돼지에 煉炭灰를 급여하였을 때 鐵分注射代用 및 微量鑛物質 供給源으로서 效果를 究明하기 위하여 본 대학 부속목장에서 실시한 결과를 綜合的으로 考察해 본 것이다. 대체적으로 姙娠中 암돼지에 煉炭灰給與區와 대조구간에 나타난 仔豚의 生時體重, 21日齡 體重, 56日齡 體重은 有意的인 差異가 없었다. 育成率은 煉炭灰給與區가 優秀하였다. 離乳後 계속 煉炭灰를 給與한 育成豚은 대조구보다 15週齡 체중, 일당증체량 및 사료효율이 유의적으로 높았다. 이상의 결과를 綜合 考察할 때 平素에 煉炭灰를 먹이면 仔豚에 鐵分注射를 아니해도 이상이 없고 育成豚은 微量鑛物質 不足으로 발생하는 여러 가지 疾病이 발생하지 아니하였으므로 煉炭灰를 먹였을 때 돼지의 성장 및 飼料費 節減에 效果가 있는 것으로 나타났다. 8. 煉炭灰 魚汁吸着飼料를 育成期에 5%, 肥育期에는 10%를 添加하여 飼養試驗하였을 때 (1) 日當 增體量은 試驗區(784.52g)가 對照區(645.24g)보다 높게 나타났다. (2) 飼料攝取量은 試驗區(185.53kg)가 對照區(165.46kg)보다 높게 나타났으며, (3) 飼料效率은 試驗區(2.82)가 對照區(3.05)보다 有意的으로 높게 나타났다. (4) 煉炭灰 魚汁吸着飼料 添加 飼養으로서 1kg 增體當 飼料費가 49.45원이 節減되었다. (5) 90kg 到達日齡은 試驗區(155日)가 對照區(169日)보다 14日이 短縮되었다. 9. 煉炭灰 魚汁吸着飼料의 代替水準(0%, 3%, 5%, 10%)別 飼養試驗成績은 (1) 育成期 煉炭灰 魚汁吸着飼料를 C(0%), T1(3%), T2(5%), T3(10%) 水準別로 代替 配合한 飼料로 飼育하였을 때 ① 日當增體量은 試驗區가 T1:658g, T2:686g, T3:683g으로 對照區의 618g 보다 높게 나타났으며, ② 1일 飼料攝取量은 T3:1.61kg>T2:1.59kg>T1:1.55kg>C:1.54kg의 順으로 魚汁吸着飼料量이 增加함에 따라 증가하였다. ③ 增體量은 T2(38.47kg)>T3(38.20kg)>T1(36.83kg)>對照區(34.59kg)의 順으로 T2區가 가장 높게 나타났고, 飼料要求率도 T2(2.32), T1(2.36), T3(2.37)區의 순으로 좋게 나타났으며 全般的으로 處理區가 對照區(2.49)에 比하여 좋은 성적을 나타내었다. ④ 1kg 增體當 飼料單價는 T2區가 291.24원으로 가장 낮게 나타났고 對照區에 比해 1kg 增體當 24.62원의 節減效果가 나타났다. ⑤ 育成期飼料의 消化率은 對照區의 處理區間에 有意적인 差異가 없었고 貯藏期間에는 成分의 變化없이 安定한 것으로 나타났다. (2) 肥育期 煉炭灰 魚汁吸着飼料를 配合飼料에 0%(C), 3%(T1), 5%(T2) 및 10%(T3) 水準으로 代替 配合하여 飼育하였을 때 ① 日當增體量은 T3(779.33g)와 T2(752g)에서 對照區(674g) 보다 높았고 (p<0.01) 飼料效率 및 90kg 到達日齡도 試驗區에서 良好한 成績을 나타내었다. ② 肥育飼料의 消化率은 多少 向上되는 傾向이나 有意的인 差異는 없었다. ③ 煉炭灰魚汁吸着飼料를 配合한 試驗區에서는 貯藏初期 7日間에 成分含量이 낮아졌으나 以後 21日까지는 安定的인 狀態를 나타내었다. ④ 背脂肪層 두께는 T3(10%)區가 1.51cm로 對照區 1.56cm보다 얇게 나타났다 (p<0.01). ⑤ 屠體率은 對照區(61.99%)에 比하여 試驗區(63.67∼64.65%)가 높게 나타났다 (p<0.01). ⑥ 1kg 增體에 所要된 飼料費는 對照區(386.81원), T1(374.19원), T2(347.79원) 및 T3(33699원)로써 試驗區의 飼料費가 절감되었다. (3) 育成-肥育期 煉炭灰 魚汁吸着飼料를 配合飼料에 0%(C), 3%(T1), 5%(T2) 및 10%(T3) 水準으로 代替 配合하여 飼育하였을 때 ① 增體量, 日當增體量, 飼料效率 및 90kg 到達日齡 等에서 對照區에 比하여 FBF를 5%(T2), 10%(T3) 代替 配合하여 飼育하였을 때 良好하게 나타났다 (p>0.01). ② 育成期 및 肥育期飼料의 消化率은 多少 向上되는 傾向이었으나 有意性은 없었다. ③ 煉炭灰魚汁吸着飼料를 代替 配合한 試驗區에서는 貯藏初期 7日間에 成分含量이 낮아졌으나 以後 21日까지는 安定的 狀態를 나타내었다. ④ 背脂肪層은 T3(10%)區가 1.51cm로 對照區 1.56cm 보다 얇게 나타났다 (p<0.01). ⑤ 屠體率은 對照區(61.99%)에 比하여 試驗區(63.67∼64.65%)가 높게 나타났다 (p<0.01). ⑥ 1kg當 增體에 所要된 飼料費는 對照區(350.77원), T1(336.32원), T2(320.89원), T3區(316.11원)의 순으로써 煉炭灰魚汁吸着飼料 添加時 飼料費가 節減되었다. 10. 煉炭灰 魚汁吸着飼料와 濃縮魚汁을 配合飼料에 各各 10%씩 代替 給與하였을 때 (1) 終了時體重과 日當增體量은 對照區(94.05kg, 678g)에 比하여 煉炭灰魚汁吸着飼料 代替區(98.65kg, 752g) 및 濃縮魚汁代替區(95.40kg, 709g)順으로 나타났다. (2) 飼料效率은 對照區에 (3.65) 比하여 煉炭灰魚汁吸着代替區(3.24) 및 濃縮魚汁 代替區(3.40)에서 良好하게 나타났다. (3) 1kg增體當 所要飼料費는 煉炭灰 魚汁代替區(369.33), 濃縮魚汁代替區(388.15), 對照區(413.56)의 順으로 나타났다. (4) 90kg到達日齡은 煉炭灰魚汁 代替區(162.1), 濃縮魚汁 代替區(163.7) 및 對照區(166.9)의 順으로 短縮되었다. 11. 煉炭灰 魚汁吸着飼料給與 飼養時의 血液狀은 赤血球, 血色素量, 赤血球容積 및 白血球數는 無處理區에서 높은 편이고, 平均赤血球容積, 平均血球血色素量, 平均赤血球血色素濃度는 煉炭灰魚汁吸着飼料 添加區에서 약간 높았으나, 白血球 鑑別計劃數를 包含한 이들 血液像은 正常値 範圍內였다. 12. 哺乳仔豚에 鐵分注射代用으로서 煉炭灰 魚汁吸着飼料를 給與하였을 때 (1) 日當 飼料攝取量은 對照區 408g 보다 處理區가 418g으로 10g이 더 높았다 (p<0.01). (2) 平均 日當增體量은 對照區 222.94g 보다 處理區가 296.89g이 더 높았다(p<0.01). (3) 飼料效率은 對照區 1.83 보다 處理區가 1.41로 改善되었다. (4) 哺乳仔豚에 鐵分注射代用으로 煉炭灰魚汁吸着飼料를 自由採食 시켰을 때 仔豚의 健康狀態의 發育成績은 比較的 良好하였다. 13. 煉炭灰 魚汁吸着飼料 代替 水準別 配合飼料製造費用은 1kg當 單價로서 育成期 配合飼料는 代替水準 0%가 125.34원, 3%가 125.56원, 5%가 126.03원 및 10%가 125.43원으로 나타났고, 肥育期飼料는 0% 113.31원, 3% 113.52, 5% 113.68, 10% 114.10원으로 나타났다 (煉炭灰 魚汁吸着飼料 1kg當 生産費 100원 基準). 以上의 結果를 보아 廢棄物인 煉炭灰와 廢水處理되는 魚汁을 混合하여 飼料資源化하므로써 飼料費節減, 飼料效率改善, 營養素利用 增進, 環境汚染防止, 公害處理, 煉炭灰魚汁吸着飼料 開發 活用으로써 導入飼料外貨 節減效果와 養畜農家의 所得增大에 이바지할 것으로 生覺된다. In Korea, 500,000M/T of fish soluble and 9,910,000M/T of briquette-ash are yearly produced and discarded as waste materials. A series of experiments was performed to develop fish soluble-absorbed briquette ash feed(FBF) in order to utilize the protein, UGF, Ca contained in the abandoned fish soluble and Fe, Zn, and other microminerals in the discarded briquette-ash. From the year 1981 to 1984 the chemical composition of briquette-ash was analyzed and its feeding effects on sucking, growing and fattening pigs were studied. Also the effects of briquette-ash addition for substituting Fe injection in pigs at sucking were examined. After the potential of briquette-ash feed was proved, from the year 1985 to 1989 fish soluble-absorbed briquette ash feed(FBF) was made and previously fed from 5 to 10%, subsequently, 3, 5, and 10% of pig ration was substituted by FBF and its effects on sucking pig as substitution for Fe injection, its feeding effects on growing, fattening and growing-fattening pigs, changes during storage, digestibility, economics, backfat thickness and hematological values were examined and gave results as follows. 1. Analysis of proximate composition and mineral contents of briquette and briquette-ash. Briquette-ash contained moisture(0.15%), C·protein (0.52%), C·fat (0%), C·fiber (4.13%), NFE (0%), C·ash (95.35%), Ca (0.807%), P (0.074%), K (0.138%), Na (0.025%), Mg (0.025%), Mg (0.183%), Fe (3,769mg/kg), Mn (98mg/kg), Zn (139mg/kg), Cu (21.0mg/kg). And in the briquette contained NFE (10.96%), C·ash (71.35%), Ca (0.224%), P (0.043%), K (0.158%), Na (0.039%), Mg (0.19%), Fe (7,385mg/kg), Mn (116mg/kg), Zn (306mg/kg), Cu (17.5mg/kg). 2. Analysis of Fish soluble-absorbed Briquette ash Feed (FBF). To study the effects of feeding Fish soluble-absorbed Briquette ash Feed (FBF) in pigs, firstly the chemical composition, mineral contents and amino acids contents of briquette ash absorbed concentrated fish solubles were analyzed. Increasing absorbed rate of concentrated fish soluble to briquette ash, chemical composition, mineral contents and amino acids contents resulted higher. 3. When the briquette-ash was fed ad libitum to sucking and growing-fattening pigs as micromineral supplements; (1) Body weight gain was little higher in the briquette-ash supplied group than control(p>0.05). (2) In the growing-fattening pig, feed efficiency was better in the control group (4.71) than the briquette-ash supplied group (4.84). (3) In the weaning pig the feed efficiency was 3.0 and suplemental effects of briquette-ash were outstanding in atrophic pigs. (4) Making economical analysis by term of feed cost per kg weight gain, in the briquette-ash supplemented group, the feed cost was increased to 852 won where it was 829 won in the control group. (5) In the weaning pig group, 528 won was used per kg weight gain. (6) The palatability of briquette-ash was good and healty conditions were normal, too. 4. When the briquette-ash was supplemented in 3%(T1), 5%(T2) and 10%(T3) to the sucking, weaning and growing pigs; (1) When the briquette-ash was fed as substitution for Fe injection for sucking pigs, the growing state and health conditions were good enough. (2) Body weight gain was best when 3% of briquette-ash was supplemented(p>0.05). (3) There was no significant difference in feed efficiency(p>0.05). (4) Feed costs required per kg weight gain for T1, T2 and T3 were 636.40, 672.52 and 684.56 won respectively. (5) Hematological values of treatment group were in normal range. 5. Effects of briquette-ash addition as substitution for Fe injection when breeding and sucking pigs are fed with briquette-ash. (1) The pig of this college (5.27kg) had slightly heavier average weight at the age fo 3 wks. than the purebred of National Livestock Breeding Station(5.00kg). (2) The purebred (8.3pigs. 88.3%) had higher litter size and survival rate at weaning than the pig of this college. (3) The pig of this college (17.8kg) had slightly heavier average weight at weaning than the purebred of National Livestock Breeding Station (17.4kg). (4) In the group equal to farrowing season and litter size at sucking, there were nonsignificant defferences for the reproductive traits between briquette-ash addition and Fe injection group. (5) When fed liberally, briquette-ash as the substitute for Fe injection in sucking pig, there is relatively superior tendency to the growth and health conditions in pigs. 6. When the Fe injection was reduced to once and briquette-ash was supplied liberally; (1) Pig weight at 21 days was heavier in the pigs feeding briquette-ash after injected Fe once than in those injected Fe twice. (2) Pig weight at weaning(16.8kg) was lower in the pigs feeding briquette-ash after injected Fe once than in those injected Fe twice (17.4kg). (3) Pig weight at 15 wks. was heavier in the pigs feeding briquette-ash after injecte Fe once (48.85kg) than in those injected Fe twice (46.0kg). (4) Final body weight was heavier in the pigs feeding briquette-ash after injected Fe once (78.3kg) than in those injected Fe twice (71.6kg). (5) Feed efficiency was better in the pigs feeding briquette-ash after injected Fe once (2.32) than in those injected Fe twice (2.34) (6) The health conditions of the pigs feeding briquette-ash injected Fe once were normal. (7) The results obtainced in this study suggest that briquette-ash feeding can substitute Fe injection effectively supplementing sufficient microminerals from growing to fattening. 7. The results of feeding briquette-ash to pigs during 4 years from 1981 to 1984 in substitution for Fe injection and as micromineral supplements are reviewed. Generally, when sows are fed briquette-ash ad libitum, there were no significant differences in birth weight, weight at 21 days and weight at 56 days. The survival rate was better in the briquette-ash supplemented group. However when the briquette-ash was supplied continuously after weaning, there were significant differences in weight at 15 days, weight gain and feed efficiency. In conclusion, it might be suggested that the pigs farrowed from sows fed ad libitum briquette-ash could grow without Fe injection and when fed briquette-ash ad libitum, it can prevent micromineral deficiency and gain body weight more rapidly. 8. When FBF was added by 5% in the growing pig ration and 10% in the fattening pig ration; (1) Daily body weight gain was higher in the treatment group (784.52g) than the control group (645.24g). (2) Feed intake was higher in the treatment group (185.53kg) than the control group (165.46kg). (3) Feed efficiency was significantly higher in the treatment group (2.82) than the control group (3.05). (4) Adding FBF in ration, 49.45 won was saved per kg body weight gain. (5) Ages at 90kg were shortened by 14 days in the treatment group (155d) than the control group (169d). 9. Effects of levels 0%(C), 3%(T1), 5%(T2), and 10%(T3) of substitution of FBF (1) Growing period 1) Daily body weight gain was higher in the treatment group (T1:658g, T2:686g, T3:683g) than the control group (618g). 2) Daily feed intake was in order of T3:1.61kg>T2:1.59kg>T1:1.55kg>C:1.54kg. it increased according to the level of fish soluble. 3) Body weight gain was in order of T2:38.47kg>T3:38.20kg>T1:36.83kg>C:34.59kg. Feed efficiency was in order of T2:2.32>T3:2.37>T1:2.36>C:2.49. Generally the treatment group was better than the control group. 4) Feed cost per kg weight gain was lowest in T2(291.24won) and it saved 24.62won per kg weight gain compared to the control group. 5) There was no significant difference in the digestibility of growing diets and it was stable during the storage period. (2) Fattening period 1) Daily weight gain was higher in T3 (779.33g) and T2 (752.0g) than control (674.0g) significantly (p<0.01). Feed efficiency and ages at 90kg were also better in the treatment group. 2) Digestibility was improved in the treatment group by not signivicantly. 3) Until the 7th day of storaging period, the chemical composition of FBF added ration decreased but it did not show any change until the 27th day. 4) Backfat thickness was significantly (p>0.01) thinner in T3 (1.51cm) than control (1.56cm). 5) Dressing percentage was significantly (p>0.01) higher in the treatment group (63.67∼64.65%) than in the control group (61.99%). 6) Feed costs required per 1kg weight gain were 386.81 won in control, 374.91won in T1, 347.79 in T2 and 336.99won in T3. As result feed costs were saved adding FBF in the ration. (3) Growing-fattening period 1) Weight gain, feed efficiency and ages at 9kg were significantly higher in FBF added T2(5%) and T3(10%) group. 2) Digestibility of growing and fattening diet was improved in the treatment group but not significantly. 3) The chemical composition of FBF added ration decreassed slightly till the day 7 of storaging period. But after 7th day it did not show and change untill the dat 27. 4) Backfat thickness was significantly (p<0.01) thinner in T3 (1.51cm) than control (1.56cm). 5) Dressing percentage was significantly (p<0.01) higher in the treatment group (63.67∼64.65%) than in the control group (61.99%). 6) Feed costs required per 1kg weight gain were 350.71won in control, 336.32 won in T1, 320.89won in T2 and 316.11won in T3. As result feed costs were saved when FBF was added in the ration. 10. When the ration was substituted by FBF and fish soluble by 10% respectively; (1) Final weight and daily weight gain were 94.05kg, 678g in control group whereas 98.65kg, 752g in FBF substituted ration and 95.40kg, 709g in fish soluble substituted ration. (2) Feed efficiency was 3.65 in control group whereas 3.24 in FBF substituted ration and 3.40 in fish soluble substituted ration giving better resuluts. (3) Feed cost required per kg weight gain was in order of FBF substituted ration (369.33 won), fish soluble substituted ration(388.15 won) and control group (413.56 won). (4) Ages at 90kg were in order of FBF substituted ration (162.1), fish soluble substituted ration (163.7) and control group (166.9). 11. Hematological values of FBF fed pigs RBC, hemoglobin, hematocrit and WBC were higher in the non-treated group, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were a little higher in the treated group. And all the above hematological values including differential count were within the normal range. 12. Effects of FBF addition as substitution for Fe injection for sucking pigs. (1) Daily feed consumption was larger by 10g in the treatment group (418g) than in the control group(408g). (2) Average daily weight gain was larger by 73.95g in the treatment group (296.98g) than in the control group (222.94g). (3) Feed efficiency was higher in the treatment group (1.41) than in the control group (1.83). (4) When the sucking pigs were fed liberally FBF as the substitute for Fe injection, there was relatively superior tendency to their growth and health conditions. 13. Production cost of FBF per kg (when the production cost of 1kg of FBF is fixed as 100 won) (1) Growing 0% ; 125.34 won, 3% ; 125.56 won, 5% ; 126.03 won and 10% ; 125.43 won (2) Fattening 0% ; 113.31 won, 3% ; 113.52 won, 5% ; 113.68 won and 10% ; 114.10 won As conclusion, making FBF with the abandoned briquette-ash and fish soluble results in lowering feed cost, increasing feed efficiency, increasing nutrient availability, decreasing pollution, saving the foreign currency used to import feed ingredients and increasing farmers income.

인간 면역부전 바이러스(HIV) 감염자 사이에서의 1기 및 2기 매독의 유행

장희창,조재현,박완범,이기덕,이창섭,김홍빈,오명돈,최강원 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 국내 HIV 감염자 사이에서 발생한 매독의 유행을 보고하고, 그 역학적 특성을 규명하고자 하였다. 재료 및 방법 : 1999년 7월부터 2003년 9월까지 서울대학교 병원에서 추적 관찰을 받아온 HIV 감염자를 대상으로 하여 1기 및 2기 매독의 발생을 조사하였다. 발생률을 정확히 구하기 위해 추적 관찰을 받은 모든 HIV 감염자의 인년을 6개월 간격으로 구하였다. 결과 : 51개월 동안, 465명의 HIV 감염자가 서울대학교 병원에서 추적 관찰을 받았다. 이중 38명이 1기 및 2기 매독으로 진단되었다. 1기 및 2기 매독의 발생률은 이기간 동안 100인년 당 4.1명이었다. 1999년 7월부터 2001년 12월 사이에는 발생자가 없었으나, 이후 발생률은 꾸준히 증가하여 2003년 9월에는 100인년 당 18.8명이 되었다. 1기 및 2기 매독의 발생률은 동성애자 및 양성애자에서 이성애자에서보다 4.3배 높았고, HAART로 치료를 받지 않은 환자에서 HAART로 치료를 받고 있던 환자에서 보다 10.9배 높았다. 결론 : 2002년부터 국내 HIV 감염자 사이에서 1기 및 2기 매독이 유행하기 시작하였고, 이러한 유행은 동성애자와 양성애자 및 HAART로 치료를 받고 있지 않던 사람 사이에서 발생하였다. Background : This study was performed to characterize the epidemiologic and clinical features of outbreak of syphilis among HIV sero-positive patients in Korea. Materials and Methods : A retrospective case review of patients diagnosed with primary and secondary syphilis from July 1999 to September 2003 was carried out at Seoul National University Hospital in Korea. To estimate the incidence, person-years (PYs) of all HIV sero-positive patients, who visited the hospital in the same period, were calculated every 6 months. Results : In a 51 month period, 465 HIV-positive patients were followed up at Seoul National University Hospital. 38 cases of primary and secondary syphilis were diagnosed. The incidence of primary and secondary syphilis was 4.1 per 100 PYs during the study period. There was no case from July 1999 to December 2001, and then the incidence rose until September 2003 from 5.5 per 100 PYs in 1999 to 18.8 per 100 PYs in 2003. The rate of primary and secondary syphilis was 4.3 times higher among homosexual and bisexual men than heterosexual men (95% CI 1.87 to 11.17), and 10.9 times higher among patients who did not receive HAART than patients who were receiving HAART (95% CI 5.47 to 21.79). Conclusion : The outbreak of primary and secondary syphilis among HIV-positive patients started in 2002 and has been escalating, especially among homosexual/bisexual men and in patients who did not receive HAART.

경북대학교 병원 교정과에 내원한 부정교합 환자의 분포 및 변동추이

권오원,경희문,곡덕부,성재현,박동옥 대한치과교정학회 1989 대한치과교정학회지 Vol.19 No.3

1795 patients who visited orthodontic department from 1979 to 1987, were surveyed on the yearly tendency of patient distribution and state of Angle's classification. The results were as follows: 1. There was increased visiting rate of patient per year and higher visiting rate in female than in male. 2. 8-15 age group was 70.3% in total visiting patients and over 20 age group was 10.8%, under 7 age group was 7.3%. 3, Class I malocclusion was 26.3%, Class II div 1 was 31.0%, Class II div 2 was 1.6% and Class III was 41.1% in total visiting pateint. 4. There was increased tendency to be received preventive treatment than fixed treatment and increased extracting rate per year in fixed treatment. 5. There was increased tendency for the number of the patient to be received orthognathic surgery.