Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China

Yan Zhang,Yali Lv,Feifei Zhang,Wenjing Zhang,Jinhong Wang,Yanyan Cui,Rongjun Wang,Fuchun Jian,Longxian Zhang,Changshen Ning 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4

Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction(PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targetingthe 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710)of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621,9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheepand 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasmaspecies in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as arelationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand theepidemiology of anaplasmosis in ruminants from China.

Effect of Different Levels of Nitrogen, Phosphorus, and Potassium on Root Activity and Chlorophyll Content in Leaves of Brassica oleracea Seedlings Grown in Vegetable Nursery Substrate

Jian Zhang,Yan Wang,Pengcheng Wang,Qi an Zhang,Congsheng Yan,Feifei Yu,Jianqun Yi,Ling Fang 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.1

Nutrients in the substrate are important for plant growth, particularly during the development of vegetableseedlings. The continued development of intensive methods to produce seedlings has made growing healthy and high-gradeseedlings a requirement for nurseries. This study evaluated the effect of nitrogen (N), phosphorus (P), and potassium(K) on the root activity, dry weight, and chlorophyll content in leaves of Brassica oleracea seedlings. D-optimal designwas used in this study because of its accuracy. A total of 10 treatments (T1–T10) were designed, and differentconcentrations were chosen in the following ranges: N (0.6-5.0 g·kg-1), P (0.2-6.0 g·kg-1), and K (0.7-7.0 g·kg-1). Our studyshows that the application of N, P, and K to the substrate significantly influenced the growth of B. oleracea seedlings. T6significantly increased root activity, root dry weight, chlorophyll content in leaves when compared with the control T1,which had added nutrient concentrations at normal levels. However, the addition of high levels of N (5.0 g·kg-1) withhigh (7.0 g·kg-1) or low (0.7 g·kg-1) levels of K (T2 and T9) not only significantly decreased the root dry weight but alsodecreased leaf area. Our results suggest that N, P, and K can be added to the substrate at an appropriate ratio togrow vigorous B. oleracea seedlings under intensive seedling production conditions. The present results give insightinto the nutrient requirements during early growth of B. oleracea seedlings in growth substrate.

Image processing based remote control with robot arm simulator

Jiafeng. Zhang,Feifei. Zhang,Masanori. Ito 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

Motion estimation provides an attractive choice to cumbersome interface devices for human computerinteraction (HCI). Worthy of note, visual recognition of hand gestures can help achieving in an easy and natural way forinteraction between human and computer. The interfaces of HCI and other virtual reality systems depend on accurate,real-time hand and fingertip tracking for association between real objects and relative digital information. They costexpensively and complicated operations make them troublesome. We are developing a real-time, view-based gesture recognition system. Optical flow is estimated and segmented into motion fragments. Using artificial neural network (ANN), it can compute and estimate the motion of gesture. Comparing with the traditional approaches, theoretical andexperimental results show that this method has more simple hardware and algorithm demands but more effectiveness. It can be used in remote control system for understanding the human body languages.

MODEL-BASED CONTROL OF SYNCHRONIZER SHIFTING PROCESS FOR TRAJECTORY TRACKING CONTROL

Yan Zhang,Han Zhao,Mingming Qiu,Feifei Qin,Bingzhan Zhang 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.4

A novel approach of the tracking control for synchronizer displacement is introduced in this paper. Based on modeling for the structure of synchronizer and shifting process, a shifting displacement tracking controller is designed by the Udwadia - Kalaba equation. The engagement rule of synchronizer combination sleeve is regarded as the trajectory constraint of the system, certain constraint force is imposed to follow this trajectory constraint, which could be obtained by the Udwadia- Kalaba equation without using Lagrange multiplier or other auxiliary variables. Specific comparative study with conventional PID control is discussed. Simulations and vehicle test results show that the shifting actuator can accurately track the desired trajectory determined by the upper layer control strategy, thus verify the effectiveness of the controller.

Lyapunov Based Robust Control for Tracking Control of Lower Limb Rehabilitation Robot with Uncertainty

Feifei Qin,Han Zhao,Shengchao Zhen,Hao Sun,Yan Zhang 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.1

This study designs a control of two-degree of freedom lower limb rehabilitation robot (LLRR) for the patient who needs the proper physical therapy after a spinal cord injury (SCI), stroke, or a surgical operation. The robot manipulator can perform specified passive exercises as well as copy exercise motions and perform them without the physiotherapist. Specifically, the uncertainties including the model uncertainty, initial condition deviation and the external disturbance are also considered. Firstly, a unilateral man-robot dynamical model is proposed based on Lagrange method. Then, we propose a Lyapunov based robust control to suppress the effect of uncertainties. The control algorithm consists of a PD feedback component and a piecewise function component. Theoretical analysis is provided to demonstrate that the controller can guarantee the uniform boundedness and uniform ultimate boundedness of the system. Moreover, the joint angle trajectory of a healthy person is explicitly obtained by the experimental platform and used as the pre-specified trajectory of the LLRR. Finally, numerical simulation is presented to illustrate the effectiveness and the trajectory-tracking control performance of the control.

Room Temperature Electroluminescence from n-ZnO:Ga/i-ZnO/p-GaN:Mg Heterojunction Device Grown by PLD

Lichun Zhang,Qingshan Li,Feifei Wang,Chong Qu,Fengzhou Zhao 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.3

The n-ZnO:Ga/p-GaN:Mg and n-ZnO:Ga/i-ZnO/p-GaN:Mg heterojunction light emitting diodes (LEDs) were fabricated by the pulsed laser deposition (PLD) technique. The blue electroluminescence (EL) of the n-ZnO:Ga/ p-GaN:Mg heterojunction LEDs is emitted mainly from the p-GaN layer instead of the n-ZnO:Ga layer, for the reason that the electron injection from n-ZnO:Ga prevailed over the hole injection from p-GaN:Mg due to the higher carrier concentration and carrier mobility in n-ZnO:Ga. On the other hand, the n-ZnO:Ga/i-ZnO/p- GaN:Mg heterojunction LEDs exhibited dominant ultraviolet-blue emission. The reason for this difference is attributed to the inserted undoped i-ZnO layer between n-ZnO:Ga and p-GaN:Mg, in which the holes from p- GaN:Mg and the electrons from n-ZnO:Ga are recombined.

Improving organic field-effect transistors based on double active layers structure

Xinan Guo,Feifei Xing,Fei Hong,Jianhua Zhang,Bin Wei,Jun Wang 한국물리학회 2010 Current Applied Physics Vol.10 No.1

Organic field-effect transistors (OFETs) were fabricated based on double active layers structure. Different substrate temperatures and thermal treatment were adopted to prepare double active layers and optimize film morphology. The grain size of organic films can be effectively controlled by the change in substrate temperature in the process of deposition. An improved device performance was obtained compared with conventional single layer devices. This result is attributed to the introduction of double active layers. We believe that this kind of optimization will simultaneously improve charge injection and transportation of OFETs.

Joint Optimization Method Combining Genetic Algorithm and Numerical Algorithm Based on MATLAB

Yanhua Guo,Feifei Liu,Ning Zhang,Tao Wang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.11

A two-bar plane truss is builtin MATLAB based on mathematical model. Then the authors use genetic algorithm toolbox to solve this problem. The parametric truss model is set up in the finite element analysis software ANSYS. It is analyzed by first-order algorithm. The comparison of two kinds results show the pure genetic algorithm doesn’t always have an advantage over other algorithms. In the end, a joint optimization method is put forward on the basis of genetic algorithm. It combines genetic algorithm based on MATLAB toolbox and numerical algorithm based on quasi-Newton method. This method is illustrated by the numerical example of the two-bar plane truss. The results show this joint optimization method can get the global optimal solution of this problem every time.

A New Concept of Diesel Engine Fuel Injection System with DDVC Hydraulic Actuator

Kyoko Narukawa,Feifei Zhang,Masanori Ito 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10

One of the most important subjects for the ship in recent years is to decrease the environmental impact by the exhaust from diesel engine. This research proposes a new composition of a simple, cheap, efficient fuel injection system by using the Direct Drive Volume Control actuator. It is well known that fuel injection pressure, injection timing and injection pattern has big influence for efficient combustion and this study shows the proposed injection system has availability to optimize those parameters.

Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol -induced liver injury in a mouse model of chronic alcohol feeding

Fengwei Tian,Feifei Chi,Gang Wang,Xiaoming Liu,Qiuxiang Zhang,Yongquan Chen,Hao Zhang,Wei Chen 한국미생물학회 2015 The journal of microbiology Vol.53 No.12

Lactobacillus rhamnosus CCFM1107 was screened for high antioxidative activity from 55 lactobacilli. The present study attempted to explore the protective properties of L. rhamnosus CCFM1107 in alcoholic liver injury. A mouse model was induced by orally feeding alcohol when simultaneously treated with L. rhamnosus CCFM1107, the drug Hu-Gan- Pian (HGP), L. rhamnosus GG (LGG), and L. plantarum CCFM1112 for 3 months. Biochemical analysis was performed for both serum and liver homogenate. Detailed intestinal flora and histological analyses were also carried out. Our results indicated that the administration of L. rhamnosus CCFM1107 significantly inhibited the increase in the levels of serum aminotransferase and endotoxin, as well as the levels of triglyceride (TG) and cholesterol (CHO) in the serum and in the liver. Glutathione (GSH), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were elevated while the levels of malondialdehyde (MDA) were decreased. The enteric dysbiosis caused by alcohol was restored by increasing the numbers of both lactobacilli and bifidobacteria and decreasing the numbers of both enterococci and enterobacter. Histological analysis confirmed the protective effect of L. rhamnosus CCFM1107. Compared with the other lactobacilli and to the drug Hu-Gan-Pian, there is a high chance that L. rhamnosus CCFM1107 provides protective effects on alcoholic liver injury by reducing oxidative stress and restoring the intestinal flora.