주사용 세팔로스포린 항생제 YH-1226의 항균스펙트럼 분석 : YH-1226과 cefpirome, cefmenoxime, ciprofloxacin의 시험관내 항균효과 비교

우준희,김백남,이종욱,류지소 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

Background and methods The in vitro activity of YE-1226, a newly developed parentral cephalosporin was compared to those of cefpirome, cefmenoxime, and ciprofloxacin against 223 clinical isolates by measuring minimal inhibitory concentration. Results . YH-1226 and cefpirome were all active against E. coli, K. pneumonzae, and C. fieuizdzz. 81% of were susceptible to cefmenoxime, 75% to ciprofloxacin. For Enterobacter cloacae 95% were susceptible to YH-1226, 83%, 65%, and 85% were susceptible to cefpiiome. cefmenoxime, and ciprofloxacin. For P. aeruginosa, 68%, 58%, 48%, and 65% were susceptible to YH- 1226, cefpirome, cefmenoxime, and ciprofloxacin, respectively. For S. epidennzdzs, 80% were susceptible to YH-1226, 70%, 60%, and 70% were susceptible to cefpirome, cefmenoxime, and ciprofloxacin. With regard to gram-positive strains except S. epidermidis, 69% were susceptible to YH-1226, 57% to cefpirome, 56% to cefmenoxime, and 50% to ciprofloxacin. Conclusion : The broad antimicrobial activity suggests that YH-1226 may be an alternative in the treatment of bacterial infections.

혈액배양검사에서 확인된 G군 연세구균에 의한 봉와직염 1예

조영환,김양수,김백남,추은주,김경덕,김남중,류지소,우준희 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.1

봉와직염은 대부분이 A군 연쇄구균이나 황색포도구균에 의해 발생하는 것으로 알려져있다. 저자들은 관상동맥 우회술을 위한 정맥절제술을 받았던 과거력이 있고 족부백선이 있었던 환자에게서 혈액배양검사에서 확인된 G군 연쇄구균에 의한 봉와직염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Group A streptococci and Staphylococcus aureus are responsible for the overwhelming majority of cases of cellulitis. But, streptococci belonging to other group (group C, group G, and in neonates particularly, group B) are sometimes the etiologic agents. Diagnosing the etiology of cellulitis rests on isolating an organism from blood cultures, skin biopsy, or tissue aspirate. We experienced a case of a group G streptococcal cellulitis that identified by blood cultures, which is relatively rare in Korea and treated the patient with cefazolin.

TREND OF VISIBILITY IMPAIRMENT CAUSED BY SMOG PHENOMENON IN SEOUL

Nam Jun Baik,Jong Hoon Lee,Yong Pyo Kim,Kil Choo Moon,Yung Il Joe 한국화학공학회 1995 Korean Journal of Chemical Engineering Vol.12 No.3

The characteristics of visual air quality in Seoul have been investigated from March to November 1993. Optical properties, meteorological parameters, and particle characteristics were measured and analyzed. On the average, light scattering by particles is the dominant process in light extinction. Fine particle mass concentration, and the fraction of sulfate, nitrate and ammonium ions in the particles were found to play a major role in influencing the occurrence of a smog episode in Seoul. The role of ambient relative humidity on Seoul visibility is discussed.

서울시에서의 빛 소멸/분산계수 측정 및 해석

백남준(Nam Jun Baik),김태오(Tae Oh Kim),김용표(Yong Pyo Kim),문길주(Kil Choo Moon) 대한환경공학회 1993 대한환경공학회 학술발표논문집 Vol.1993 No.12

학술발표회 초록 ( 에너지 / 환경 (Ⅲ) ) : 서울시의 1993년 가을 스모그 모사 연구

백남준(Nam Jun Baik),이성준(Sung Jun Lee),김용표(Yong Pyo Kim),문길주(Kil Choo Moon) 한국화학공학회 1994 춘계학술발표회 Vol.- No.-

Chryseoglobus frigidaquae gen. nov., sp. nov., a novel member of the family Microbacteriaceae

Baik, Keun Sik,Park, Seong Chan,Kim, Ho Jun,Lee, Kang Hyun,Seong, Chi Nam Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.6

<P>A motile, rod-shaped, yellow-pigmented bacterium, designated strain CW1<SUP>T</SUP>, was isolated from a water-cooling system in the Republic of Korea. Cells were Gram-stain-positive, aerobic, catalase-positive and oxidase-negative. Strain CW1<SUP>T</SUP> formed slender rods with unusual bulbous protuberances. The major fatty acids were iso-C16 : 1 (33.7 %), anteiso-C15 : 0 (27.2 %), iso-C14 : 0 (13.3 %) and C16 : 0 (10.8 %). The cell-wall peptidoglycan was of type B2<I>β</I>, containing lysine as the diamino acid. The respiratory quinones were menaquinones with 12, 13 and 14 isoprene units. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW1<SUP>T</SUP> formed an evolutionary lineage within the radiation enclosing members of the family <I>Microbacteriaceae</I> and was related to, but distant from, members of the genera <I>Microcella</I> and <I>Yonghaparkia</I>. On the basis of the evidence presented, strain CW1<SUP>T</SUP> is considered to represent a novel species of a new genus in the family <I>Microbacteriaceae</I>, for which the name <I>Chryseoglobus frigidaquae</I> gen. nov., sp. nov. is proposed. The type strain of <I>Chryseoglobus frigidaquae</I> is CW1<SUP>T</SUP> (=KCTC 13142<SUP>T</SUP> =JCM 14730<SUP>T</SUP>).</P>

비만증에서 성장호르몬 치료효과에 따른 성장호르몬-결합단백의 변화

남수연,손주익,김경욱,박우일,김준우,박운형,윤승백,송기섭,김경래,송영득,임승길,이현철,허갑범 대한비만학회 2000 The Korean journal of obesity Vol.9 No.2

연구배경 : 비만인에서 성장호르몬은 체지방의 분해를 촉진시키고 동화작용을 증강시킨다. 또한 성장호르몬 치료는 내장지방량을 감소시킴으로써 내장지방형 비만과 관련된 대사성 합병증을 줄일 수 있음이 밝혀져 있다. 사람의 성장호르몬 결합단백은 성장호르몬 수용체의 세포외 부분이 단백분해에 의해 분리되어 혈중에서 순환하는 물질로서 혈중 성장호르몬 결합단백의 농도는 성장호르몬 수용체의 활성도를 간접적으로 반영해 주는 물질로 알려져 있다. 성장호르몬 - 결합단백의 혈중 농도는 비만인에서 증가되어 있으며 내장지방량과 매우 높은 양성의 연관 관계를 보인다. 본 연구에서 저자등은 비만인에서 식사제한과 성장호르몬투여 병용요법시 성장호르몬-결합단백의 변화를 알아보고자 하였다. 방법 : 18명의 비만여성 실험군과 8명의 정상체중여성 대조군에서 성장호르몬-결합단백, 경구 당부하 검사 후 인슐린, 유리지방산, 인슐린양 성장인자-I의 농도, 전산화단층 촬영을 이용한 피하지방과 내장지방량을 측정하였다. 18명의 비만군은 각각 12주간 성장호르몬 (n=9, 0.03 IU/kg IBW/day)과 위약 (n=9, vehicle injection)으로 치료받았으며 동시에 하루 열량을 표준 체중 1kg당 25kcal의 저열량 식사요법을 시행하였다. 결과 : 이상에서 다음의 결과를 얻었다. 1. 정상체중의 대조군과 비교하여 비만군에서는 성장호르몬-결합단백의 농도가 증가되어 있었고 치료 전 두 비만군의 성장호르몬-결합단백의 농도는 비슷한 수준이었다 (성장호르몬군, 2.24±0.51; 위약군, 2.22±0.27; 대조군, 1.04±0.20 ng/mL) 2. 12 주의 치료 뒤의 두 실험군의 체중감소량은 유의한 차이가 없었다. (성장호르몬군, 6.01±2.14; 위약군, 5.47±3.56 kg). 총 체중감소량에서 지방조직의 감소가 차지하는 부분은 성장호르몬치료군이 위약군에 비해 유의하게 (1.20±0.51 vs 0.65±0.44 kg, p<0.05) 높았으며 내장지방량의 감소는 성장호르몬치료군에서 위약군보다 현저한 감소를 보였다 (27.9% vs 21.6%, p<0.05). 12주 치료 후 성장호르몬 치료군의 성장호르몬 - 결합단백농도는 치료전과 비교하여 유의한 감소 (1.90±0.13 vs 1.55±0.14 ng/mL)를 보였으나 정상대조군의 성장호르몬- 결합단백농도 보다는 높았다 (성장 호르몬군, 1.55±0.41 vs 대조군 , 1.04±0.20 ng/mL, p<0.05). 이와는 대조적으로 위약군에서의 성장호르몬-결합단백의 농도는 치료 후 감소하는 경향을 보이긴 했으나, 통계적으로 유의한 감소를 나타내지는 못했다. (2.22±0.27 vs 1.91±0.39 ng/mL). 3. 치료 후 성장호르몬-결합단백의 농도의 변화는 총 체지방량, 내장지방량, 유리지방산-분비반응면적의 감소와 양의 상관관계를 보였다. 인슐린양-성장인자-I과인슐린-분비반응면적과 성장호르몬-결합단백의 농도변화는 연관관계가 업었다. 4. 다중 선형 회귀 분석 결과 성장호르몬 치료 후 내장지방량의 변화가 성장호르몬-결합단백의 농도 감소에 64%의 기여함을 보였다. 결론 : 비만증에서 증가된 성장호르몬-결합단백의 농도가 저열량 식사요법과 저용량 성장호르몬 치료를 통한 체중감량에 의해 가역적으로 감소할 수 있음을 확인하였다. 또 비만증의 성장호르몬 치료 후 기대되는 내장지방량의 감소와 제근육량의 증가는 주로 치료전 성장호르몬-결합단백의 농도에 의해 결정됨을 보여주었다. 이러한 일련의 관찰 결과를 종합할 때 성장 호르몬에 의해 성장호르몬-결합단백의 농도가 조절된다기 보다는 오히려 이와는 반대로 이미 존재하는 성장호르몬- 결합단백의 농도가 성장호르몬의 치료효과를 결정하는 것임을 시사한다고 하겠다. Background : Growth hormone (GH) can play a possible therapeutic role in obesity by accelerating lipolysis and exerting an anabolic effect. Considering that the serum GH-binding protein (GHBP) level may reflect the GH receptor, the elevated levels of GHBP in obesity may indicate up-regulation of the GH receptor and increase GH sensitivity. Serum GHBP level is associated with body fat amount. It is not certain how GHBP level can be changed by GH treatment with diet restriction in obese subjects. Methods: We measured serum GHBP, insulin and free fatty acid (FFA) during oral glucose tolerance (OGTT) and IGF-I level as well as visceral and subcutaneous adipose tissue (AT) areas by using CT scan in 18 obese subjects and in 9 age0matched healthy lean subjects. The 18 obese subjects were treated with GH (n=9, 0.03 IU/kg IBW/day) or placebo (n=9, vehicle injection) for 12 weeks and were fed a diet of 25 kcal/kg IBW/day. Results : 1. The GHBP levels were elevated in obese subjects compared to lean controls and were similar in both obese groups before treatment (GH, 2.24±0.51; placebo, 2.22±0.27; control, 1.04±0.20 ng/mL). 2. After 12 week-treatment, the loss of BW was not different in both groups (GH, 6.01±2.14; placebo, 5.47±3.56 kg). The fraction of BW loss as fat loss was significantly greater (1.20±0.51 vs 0.65±0.44 kg/kg, p<0.05) and visceral fat was decreased more in GH group than in placebo group (27.9% vs 21.6%, p<0.05). After 12 week treatment, GHBP level in GH group was significantly decreased but not to the level of normal control (GH 1.55±0.41 vs control, 1.04±0.20 ng/mL, p<0.05) whereas GHBP level in placebo group tended to decrease after treatment, but did not reach significance (2.22±0.27 vs 1.91±0.39 ng/mL). 3. The treatment-induced changes in GHBP levels were positively correlated with decreases in total body fat, visceral AT area and FFA-area under the curve (AUC). NO correlation was found between changes in GHBP level and changes in IGF-I and insulin-AUC. 4. Multiple stepwise regression analysis revealed that changes in visceral AT area and total body fat amount after treatment were accounted for 64% of fall in GHBP levels. Conclusion : Increased GHBP levels in obesity are reversible weight loss resulting from low-dose GH treatment and diet restriction. GH exposure does not substantially regulate GHBP levels but the decrease in visceral fat amount mainly related to the changes in GHBP level.

Production of Poly-3-hydroxybutyrate from Xylose by Bacillus megaterium J-65

Jun Hong-Ki(전홍기),Young-Hi Jin(진영희),Hae Nam Kim(김해남),Yun-Tae Kim(김윤태),Sam Woong Kim(김삼웅),Hyung Suk Baik(백형석) 한국생명과학회 2008 생명과학회지 Vol.18 No.12

본 연구는 생분해성 플라스틱인 poly-β-hydroxybutyrate (PHB)의 생산단가를 낮추기 위한 노력으로 저가의 기질로부터 PHB 대량생산을 위한 기초자료를 얻는데 그 목적을 두었다. Hemicellulose hydrolysate는 지구상에 풍부하게 존재하는 저가의 waste by-product로서 xylose가 많이 포함되어 있다. 본 연구에서는 xylose로부터 PHB를 생산할 수 있는 균주를 토양에서 분리하여, 분류학적 위치를 밝히고, 균체 생육 최적 조건, PHB 생산을 위한 최적 발효 배양 조건, PHB의 구조 확인 등을 검토 하였으며, 그 결과는 다음과 같다. 토양으로부터 분리한 균주 J-65는 형태학적, 배양적, 생화학적 및 partial 16S rRNA sequence에 근거하여 Bacillus megaterium로 동정하였다. B. megaterium J-65의 균체 생육 최적 조건은 온도 37℃, 초발 pH 8.0이었으며 2% xylose, 0.25% (NH₄)₂SO₄, 0.3% Na₂HPO₄ㆍ12H₂O, 0.1% KH₂PO₄였다. PHB 축적에 영향을 미치는 요인을 검토하기 위해 균체생육 최적배지에서 37℃, 24시간 1차 배양한 후, 균체를 회수하여 각종 영양분이 결핍된 배지에 2차 배양을 실시한 결과 B. megaterium J-65는 균형생육조건(balanced-growth condition)에서 PHB를 합성하는 균주로 나타났다. PHB보다 물성이 향상된 PHB/HV 공중합체를 생산하기 위하여 보조기질로 propionic acid를 첨가하였을 때, 0.1% propionic acid 농도에서 HV ㏖%가 14%인 PHB/HV 공중합체가 합성되었다. 5 l 용량의 발효조에 B. megaterium J-65를 회분배양하였을 때 배양 21시간에 건조균체량 10 g/l, PHB 3.5 g/l를 얻을 수 있었고, 유가배양을 실시한 결과 배양 48시간에 건조균체량 26.52 g/l, PHB 9.28 g/l를 얻을 수 있었다. 생산된 PHB를 alkaline solution 처리와 chloroform을 이용한 유기용매 추출법을 이용하여 추출ㆍ정제한 후 Gas Chromatography로 정제를 확인하고 300㎒ 1H-NMR을 실시한 결과 3-hydroxybutyrate의 homopolymer임을 확인하였다. A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were 37℃ and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% (NH₄)₂SO₄, 0.3% Na₂HPO₄ㆍ12H₂O, and 0.1% KH₂PO₄. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of β-hydroxybutyric and β-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.

Zhihengliuella aestuarii sp. nov., isolated from tidal flat sediment.

Baik, Keun Sik,Lim, Chae Hong,Park, Seong Chan,Choe, Han Na,Kim, Ho Jun,Kim, DuWoon,Lee, Kang Hyun,Seong, Chi Nam Society for General Microbiology 2011 International journal of systematic and evolutiona Vol.61 No.7

<P>A Gram-stain-positive, ovoid to short rod-shaped, yellow-pigmented bacterium, designated strain DY66(T), was isolated from tidal-flat sediment collected from Deukryang Bay (Republic of Korea), and its taxonomic position was investigated by using a polyphasic approach. Strain DY66(T) grew optimally at 30 °C and pH 8-9 and in 2???% (w/v) NaCl. The peptidoglycan type was A4관, L-Lys-L-Ala-D-Glu, and tyvelose and glucose were the major cell-wall sugars. The predominant menaquinones were MK-10 and MK-9. Major cellular fatty acids (>10???% of total) were anteiso-C(15???:???0) and iso-C(15???:???0). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, an unknown aminophospholipid and three unknown aminolipids. The DNA G+C content was 59.1 mol%. This chemotaxonomic profile supported the assignment of strain DY66(T) to the genus Zhihengliuella. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that strain DY66(T) belonged to the family Micrococcaceae and was related to the genus Zhihengliuella. On the basis of the evidence presented in this study, strain DY66(T) represents a novel species of the genus Zhihengliuella, for which the name Zhihengliuella aestuarii sp. nov. is proposed. The type strain is strain DY66(T) (???=???KCTC 19557(T) ???=???JCM 16364(T)).</P>

Localization of Cyclooxygenase Isozymes in Skin Wound Healing in Mouse

고준혁(Jun Hyuk Koh),조백현(Bek Hyun Cho),허탁(Tag Heo),배미옥(Mi Ok Bae),이송은(Song Eun Lee),안규윤(Kyu Youn Ahn),배춘상(Choon Sang Bae),김백윤(Baik Yoon Kim),박성식(Sung Sik Park),남광일(Kwang Il Nam) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.2

Cyclooxygenase (COX)-1 and -2 expressions in the incisional wound healing of mouse skin were determined by immunohistochemistry and Western blot analysis. By Western blotting, compared to normal skin, COX-2 activity was increased at days 1, 4, 8, and 12 and was maximal at 4 day after incisional wound of mouse skin whereas COX-1 was barely detectable. In normal skin, COX-1 immunostaining was observed among the basal cells of epidermis whereas COX-2 immunostaining was detected in the more differentiated, suprabasal keratinocytes. At 1~4 days after wound, COX-2 staining was particularly prominent in the inflammatory cells, and at day 8, many macrophage-like cells were stained positively. COX-2 immunoreactive fibroblast, macrophage-like cells, and newly formed vascular endothelial cells were increased in number at 12 days after incision. These data suggest that COX-2 is constitutively expressed, just as is COX-1, in epidermis and is associated with keratinocyte differentiation. In addition, these findings support the well-established role for COX-2, the prostaglandins that they generate, as mediators of inflammatory response. 생쥐 피부상처 치유과정에서 cyclooxygenase(COX)-1과 COX-2의 분포 및 역할을 Western blot 분석과 면역조직화학 염 색을 통하여 알아보고자 하였다. Western blotting 실험에서 COX-2 단백질은 정상 피부에 비해 손상 후 1일부터 증가하여 4일째 최고치를 나타내며 12일 까지 증가한 반면에, COX-1은 검출이 되지 않을 정도의 적은 단백량을 나타내었다. 면역조직화학 염색에서 COX-1은 정상 및 손상 피부의 표피 바닥층과 털주머니의 윗부위에서 드물게 양성 반응을 나 타내었다. COX-2는 정상 및 손상군 표피에서 바닥층 위의 각질세포와 털주머니의 윗부위 및 진피유두에서 양성이었다. 손상 후 1일과 4일째 진피의 염증세포에서 COX-2에 강한 양성 반응을 나타냈고, 8일째는 큰포식세포에서, 12일째는 큰포 식세포, 섬유모세포와 신생혈관 내피세포에서 양성 반응을 보였다. 이상의 결과로서, COX-2가 COX-1과 마찬가지로 표피에 상재하는 구성요소로서 각질세포의 분화를 조절할 것임을 암 시하였고, 진피에서는 염증과 같은 자극에 대한 반응을 매개함을 확인할 수 있었다.