필리핀 여행 후 발생한 쯔쯔가무시병 1예

김계형,김낙현,김문석,김충종,전재현,박완범,장원종,박상원,김익상,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Infectious diseases imported from other countries have increased as more and more Koreans are going abroad for various purposes. Tsutsugamushi disease from other endemic area such as Southeast Asia is important, because it can occur in any season and eschar may be absent. We report a case of imported tsutsugamushi disease acquired in the Philippines. A patient presented with fever, headache, and maculopapular skin rash. However, eschar was absent. Polymerase chain reaction (PCR) for 56-kDa gene of Orientia tsutsugamushi using buffy coat was positive. Serum indirect immunofluorescent antibody assay was initially negative but became positive with a titer of 1:320 at follow-up, Sequencing analysis revealed the strain to be 100% identical to the TW73R strain identified in Taiwan. After the patient received doxycycline, body temperature normalized in 12 hours. Tsutsugamushi disease is one of the differential diagnoses that should be included for patients with fever who have recently returned from Southeast Asian countries. PCR for O. tsutsugamushi using patient's buffy coat was useful for early diagnosis.

중추신경계 합병증을 동반한 삼일열 말라리아 1례

김문석,김가연,강유민,김낙현,전재현,박완범,김홍빈,김남중,박상원,홍윤호,오명돈 대한감염학회 2009 감염과 화학요법 Vol.41 No.5

Plasmodium vivax malaria is an endemic disease in Korea, which rarely causes severe complications including those occurring in the cerebrum. There are limited numbers of complicated cases that have been reported around the world. We experienced a case of vivax malaria with cerebral complication: cognitive impairment and ataxia. A 55-year-old female with diabetes mellitus presented to the emergency department with acute fever of two days’ duration. She did not have any history of travelling abroad or receiving blood transfusions. Peripheral blood smear revealed vivax malaria with parasitemia density of 0.53 percent. She demonstrated loss of orientation, especially regarding time and place, and ataxia. Although the initial hydroxychloroquine treatment for malaria was successful, cognitive impairment and ataxia persisted and were not recovered. Brain MRI showed no structural abnormality. Brain PET showed diffuse hypometabolism in right parieto-temporal lobe of the brain.

Mycobacterium mageritense에 의한 당뇨병성 족부 감염증 1예

김충종,김낙현,김문석,김계형,전재현,박문석,박경운,박완범,박상원,김홍빈,김남중,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Diabetic foot infection is one of the important complications in patients with advanced diabetes mellitus. Limb threatening infections such as osteomyelitis, abscess, and necrotizing fasciitis are frequently accompanied by the disease. Non-tuberculous mycobacterium (NTM) is a rare causative organism of diabetic foot infection. Thus, if one is not suspicious or meticulous, infection due to NTM will be easily overlooked and this will result in delayed diagnose and treat. Therefore, it is necessary to consider NTM as the causative organism if the wound does not respond to the conventional antibiotic treatment and the culture from the adequately obtained specimen reveals atypical acid-fast bacilli. We present a case of diabetic foot infection with osteomyelitis and abscess due to Mycobacterium mageritense, one of the rapid growing mycobacteria, that was successfully treated with surgical debridement and appropriate antibiotic treatment.

에스트로겐이 진피섬유아세포의 증식 및 교원질합성에 미치는 영향

원창훈,한승규,성낙관,김우경 大韓成形外科學會 2004 Archives of Plastic Surgery Vol.31 No.3

PLSI 위상차 제어기에 의한 초음파 모터의 점탄성 특성

김동옥,김원배,성도생,고낙용,최한수,김영동 조선대학교 생산기술연구소 1996 生産技術硏究 Vol.18 No.1

As an actuator for direct drive(DD) manipulator. the travelling-wave type ultrasonic motor(USM) has many excellent performances and good advantages over conventional servo motors such as compact size, light weight, silent motion, low rotation-speed, high torque, low magnetic noise, high speed response, flexible free forms, large holding torque and so on. So it has been expected to be used as servo motor. This paper presents a design method of USM controller to adjust the phase difference of USM power using PLSI (Programmable Large Scale Intersration). we can control the elastic coefficient and the viscous coefficient of the USM by adjusting the phase difference of the motor power. By controlling the elasticity and viscosity, a robot can work in compliance with external environment. To use the result, we will be able to control the robot to reach its goal with compliance motion. It remains for further research to develop the impedance control of USM and to make cooperative robots.

Bacillus subtilis JK-56이 생산하는 chitinase isozyme의 정제와 특성 규명

김낙원,정영기,전홍기 부산대학교 유전공학연구소 2002 분자생물학 연구보 Vol.18 No.-

토양으로부터 chitinase를 생성하는 균주를 분리하여 동정한 결과 Bacillus subtilis로 판명되었으며, 분리한 균주를 Bacillus subtilis JK-56이라 명명하였다. B. subtilis JK-56의 chitinase 생산 최적 조건을 검토한 결과 1% chitin, 0.5% polypeptone, 0.1% KCl, 0.05% MnSO_4 · 4H_2O이며 초발 pH 7.0, 배양온도 37℃에서 가장 많은 효소를 생산하였다. 본 균주가 생산하는 chitinase를 정제하기 위해서 native-PAGE를 이용해 효소활성 band를 확인한 결과, 1개의 강한 활성 band와 2개의 약한 활성 band를 가지는 isozyme중 1개의 강한 활성 band를 정제하였고 정제된 효소를 Chi-56A라고 명명하였다. Chi-56A의 효소 특성에 관해서 실험한 결과 분자량은 약 53kDa, pl는 4.3으로 확인되었다. 본 효소는 65℃까지 상당히 안정하였으며 효소의 최대활성 온도도 65℃로 확인되는 등 열에 대해 상당히 안정한 효소로 확인되었다. Collidal chitin에 대한 정제효소 Chi-56A의 K_m값은 17.33g/L였다. 그리고 pH 6.0에서 최대의 활성을 나타내었고, 산성범위보다 알칼리범위에서 안정한 것으로 나타났다. 또한 Mn^2+ 존재 하에서 높은 활성을 나타내었고 Co^2+와 Mg^2+ 존재 하에서도 활성이 약간 증가한 반면에 Hg^2+ 존재 하에서는 상당한 저해를 받았다. Chito 올리고당에 대한 분해 산물을 HPLC로 확인해 본 결과 짝수개의 올리고당의 분해산물은 (GlcNAc)_2만을 생산하였고 홀수개의 올리고당에 대해서는 GlcNAc와 (GlcNAc)_2를 생산하는 것으로 비환원성 말단으로부터 이당체인 diacetyl chitobiose ((GlcNAc)_2)를 생산하는 exo형 chitinase로 추정된다. Chitin, a β-1,4 polymer of N-acetyl-D-glucosamine, is one of the most abundant organic compounds in nature. Chitinase (EC 3.2.1.14) is an enzyme that degrades chitin to chito-oligosaccharides, diacetyl chitobiose and N-acetyl-D-glucosamine. An extracellular chitinase-producing bacterial strain was isolated from soil and named to as Bacillus subtilis JK-56. Optimum culture condition of B. subtilis JK-56 for the production of chitinase was 1% chitin, 0.5% polypepton, 0.1% KCl, 0.05% MnSO_4 · 4H_2O, 37℃, initial pH 7.0 and 40 hour culture time. When B. subtilis JK-56 was grown in the optimum medium, one major active band and two minor active bands were detected by native-PAGE and active staining of the gel. Among them, the major band was purified from the culture supernatant by 70% ammonium sulfate precipitation and native-PAGE with BIO-RAD Model 491 Prep-Cell and named as Chi-56A. Its molecular weight was estimated to be 53kDa monomer and the isoelectric point (pl) was pH 4.3. The pH and temperature for the optimum activity of Chi-56A were pH 6.0 and 65℃, respectively. Chi-56A was stable up to 65℃ and in alkaline region. Its K_m value for colloidal chitin was 17.33g/L. HPLC analysis of the reaction products confirmed that Chi-56A was an exo type chitinase.

콜레라독소의 피하투여에 의한 생쥐의 림프절 및 비장내 B 및 T 림프구의 분포변화

이원규,박낙진,김종원,허영범,김정혜,안희경 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.2

CCD카메라를 이용한 3차원 데이터의 획득 및 가공

김영동,고낙용,김아현,강원찬,정헌 조선대학교 에너지.자원신기술연구소 2003 에너지·자원신기술연구소 논문지 Vol.25 No.1

Accurate acquisition of surface geometries such as machined surfaces, biological surfaces, and deformed parts have been very important technique in scientific study an engineering, expecially for system design, manufacturing and inspection. Two camera method keeps accuracy more than double than mechanical method. In this paper, we describe the processes surface data of teeth for automatic processing, transformation that changes to coping data of possible numeric data form, and processed inlay teeth. As the result of the inlay manufacture, which can process with high resolution, therefore we construct the automatic processing system that depends on manual.

유방암의 뇌하수체 전이에 의한 양안 시력소실 1 예

최낙원,안명주,김재민,박동우,박용욱 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.1

Pituitary gland metastasis is a rare clinical diagnosis, usually made in the setting of known primary systemic cancer with widespread metastasis to other organs. But visual loss as a presenting symptom of metastasis to the pituitary gland is unusal. We report here a case of 45-year-old woman who had been diagnosed as having metastatic breast cancer involving bone and liver 2 years ago, and received high dose chemotherapy with peripheral blood stem cell transplantation, developed sudden bilateral complete visual loss. At initial presentation, the patient had no light perception and no pupillary response to light in both eyes, but without evidence of panhypopituitarism, or diabetes insipidus. Brain MRI showed a large enhanced mass invasion to the cavernous sinus and extension to the suprasella portion without dura or base-of-skull metastasis. Pathologic examination showed metastatic breast cancer. Despite the transsphenoidal decompression surgery of metastatic tumor, the visual loss was not improved.

사후분해 시체에 대한 법의학적 접근

최영식,이상용,김유훈,조갑래,이봉우,양경무,정낙은,서중석,이한영,이원태,강현욱 大韓法醫學會 2000 대한법의학회지 Vol.24 No.2

Disposal of a homicide victim by dismemberment is rare, but individual cases are on record in most major medicolegal departments. Recognition of postmortem mutilation may be of importance in the interpretation of certain murders committed by sexual perverts and other mentally deranged individuals and sometimes performed for the sole reason of easier disposal of the body. Postmortem dismemberment is usually readily recognizable as such; The edges of the injuries are dry and lack evidence of bleeding. The joints may be disarticulated without fracture, or the use of an axe or saw may be evident from examination of bones. Parallel horizontal or oblique furrows in the bone surface are caused by skipping of the saw prior to establishing depth. Such patterns on the bone may assist in identifying the particular saw involved. So we report 25 dismembered corpses that autopsied in National Institute of Scientific Investigation. This paper can help in attempting to establish not only the first criminal investigation steps but also the medicolegal approach methods in unidentified and dismembered deaths.