황색계 식물성 염료중 치자와 울금에 관한 비교연구

박후나,차옥선 漢陽大學校 韓國生活科學硏究所 1994 韓國 生活 科學 硏究 Vol.- No.12

The Purpose of this study is to investigate the vegetable yellow dyes with gardenia and curcuma. After extraction of dyes from gardenia and curcuma, the concentrations of two dyes were compared through light absorbancy comparision. Dyeing effect(△K /S), color, wash fastness, light fastness, drycleaning records were also compared by observations according to the different variations of dyeing temperatures(30℃, 50℃, 80℃) and mordanting agents(Aluminium Potassium, Potassium Chromate) The result of this study are summarized as follows : 1.Gardenia dyes have higher yellow color concentration than curcuma dyes. 2.Dyeing effect Effect of dyeing temperature △K /S value is the highest at 50℃ Effect on materials : △K/S value is higher on silk than on cotton Gardenia dyes have higher △K /S value on silk, while curcuma dyes have higher value on cotton. 3.In Munsell system, the hue of gardenia dyes is 10YR and that of curcuma dyes is 5Y. Curcuma dyes have relatively higher value than gardenia dyes have. Compared with cotton, silk has higher chroma but lower value. At higher dyeing temperature gardenia dyes have lower chroma, while curcuma dyes have higher chroma with lower value 4.Color fastness test : 1) Wash fastness Gardenia dyes mark best fastness at dyeing temperature 80℃. With gardenia dyes, Cr mordanting agent marks better fastness than Al mordanting. On materials cotton has better than silk. Curcuma dyes mark best fastness at dyeing temperture 30℃. With curcuma dyes, Cr mordanting marks better fastness than Al mordanting. Curcuma dyes have, compared with gardenia dyes, better wash fastness on silk, while gardenia dyes have better fastness on cotton. 2) Light fastness Both dyes, gardenia and curcuma mark the grade I. 3) Drycleaning Gardenia dyes mark best fastness at dyeing temperature 80℃. Gardenia dyes make better fastness on cotton with Al mordanting. But on silk, non-mordanting treated materials make relatively better effect. Curcuma dyes mark best fastness at dyeing temperature 30℃. Curcuma dyes mark better fastness on cotton with Al mordanting. But on silk, non-mordanting treatment method makes relatively better fastness effect.

Localization of QTLs for Husk Proportion and Lignin Content Using a High-Density Genetic Linkage Map of Brassica napus

Fu You Fu,Lie Zhao Liu,You Rong Chai,Li Chen,Tao Yang,Ai Fen Ma,Cun Ming Qu,Lin Jiang,Zheng Sheng Zhang,Jia Na Li 한국유전학회 2007 Genes & Genomics Vol.29 No.3

Husk proportion (HP) and lignin content (LC) are two important traits of seed quality of B. napus. Efficient selection of these two traits is an important way to improve the seed quality in meal improvement. A set of recombinant inbred lines (RILs) of B. napus (GH06 × Zhongyou 821) was used for mapping QTLs of HP and LC in a two-year study in different environments. 509 markers (78 SSR markers, 66 RAPD markers and 365 SRAP markers) were mapped on 26 linkage groups with an average length of 73.96 cM. The map covered a total of 1923 cM, and the average distance between two adjacent markers was 3.78 cM. Analysis of variance of LC and HP indicated that. LC might not be significantly affected by environment (F = 2.96 < 3.02) and HP might be affected by genotype × environment interactions. A total of 15 significant QTLs were detected in eight linkage groups with a LOD threshold value of 2.5 (LR ≥ 11.5) by CIM, explaining 4.99-16.14% of phenotype variation. Some QTLs of LC and HP were detected in near regions of the same linkage groups, such as qLCBB05-6-1 and qLCWZ06-6-1, qLCBB05-10-2 and qLCBB06-10-1 and qLCWZ06-10-2, qHPBB05-9-3 and qHPBB06-9-1, qHPBB05-10-4 and qHPBB06-10-2 and qHPBB06-10-3 and qHPWZ06-10-1. This study indicates that the repeatedly detected QTLs of LC and HP might be major-effect QTLs of LC and HP.

Vibrational Energy Properties of Twin–Block Ballastless Track with Anti–vibration Structure on Bridge by Power Flow Analysis

Na Fu,Zhenhang Zhao,Yutao Liu,Yuxiang Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2

This article aims to investigate the vibrational energy properties of the twin–block ballastless track with anti–vibration structure (TBTAS) by mobility power flow method. Power flow of the components in TBTAS and bridge are deduced and power flow transfer rate is proposed to present the vibrational energy transmission property of the TBTAS–bridge system. Power flow of rail, twin–block slab and bridge in frequency domain is calculated based on a train–track–bridge interaction model. The effect of the stiffness of the elastic pad on power flow is discussed. The results show that the power flow of the rail exhibits a broad peak in the frequency range of 100 − 400 Hz which corresponds to the broad peak of the rail mobility and exhibits another peak around 1,000 Hz which is the pinned–pinned vibration frequency of the rail. By using elastic pad, the power flow of the bridge reduces by a maximum of 43 dB in the frequency range of 1 − 1,000 Hz. The power flow transfer rate shows that the vibrational energy concentrates in the slab. The vibrational energy of slab and bridge reaches the balance when the stiffness of the elastic pad is 40 MPa/m.

ACT001 alleviates inflammation and pyroptosis through the PPAR-γ/NF-κB signaling pathway in LPS-induced alveolar macrophages

Fu Qiang,Shen Na,Fang Tao,Zhang Hewei,Di Yanbo,Liu Xuan,Du Chao,Guo Jianshuang 한국유전학회 2024 Genes & Genomics Vol.46 No.3

Background ACT001 is an anti-inflammatory agent that has been widely investigated for its role in tumors, intracranial diseases, and fibrotic diseases, but its effect on acute lung injury is less known. Objective The purpose of this study was to investigate the effect and mechanism of ACT001 on regulating inflammation and pyroptosis in lipopolysaccharide (LPS)-induced alveolar macrophages. Methods NR8383 alveolar macrophages treated with LPS were used to replicate the proinflammatory macrophage phenotype observed during acute lung injury. After ACT001 treatment, we measured the secretion and expression levels of critical inflammatory cytokines, the rate of pyroptosis, and the expression of NLRP3 inflammasome-associated proteins and pyroptosis-associated proteins. In addition, we assessed the role of the PPAR-γ/NF-κB signaling pathways and further validated the results with a PPAR-γ inhibitor. Results Our findings confirmed that ACT001 reduced the expression and release of inflammatory factors, attenuated cell pyroptosis, and downregulated the expression of NLRP3, ASC, caspase-1 p20, and GSDMD-N. These effects may be achieved by activating PPAR-γ expression and then inhibiting the NF-κB signaling pathway. When macrophages were treated with the PPAR-γ inhibitor, the protective effects of ACT001 were reversed. Conclusion ACT001 significantly ameliorated inflammation and pyroptosis via the PPAR-γ/NF-κB signaling pathways in LPS-induced NR8383 alveolar macrophages. Background ACT001 is an anti-inflammatory agent that has been widely investigated for its role in tumors, intracranial diseases, and fibrotic diseases, but its effect on acute lung injury is less known. Objective The purpose of this study was to investigate the effect and mechanism of ACT001 on regulating inflammation and pyroptosis in lipopolysaccharide (LPS)-induced alveolar macrophages. Methods NR8383 alveolar macrophages treated with LPS were used to replicate the proinflammatory macrophage phenotype observed during acute lung injury. After ACT001 treatment, we measured the secretion and expression levels of critical inflammatory cytokines, the rate of pyroptosis, and the expression of NLRP3 inflammasome-associated proteins and pyroptosis-associated proteins. In addition, we assessed the role of the PPAR-γ/NF-κB signaling pathways and further validated the results with a PPAR-γ inhibitor. Results Our findings confirmed that ACT001 reduced the expression and release of inflammatory factors, attenuated cell pyroptosis, and downregulated the expression of NLRP3, ASC, caspase-1 p20, and GSDMD-N. These effects may be achieved by activating PPAR-γ expression and then inhibiting the NF-κB signaling pathway. When macrophages were treated with the PPAR-γ inhibitor, the protective effects of ACT001 were reversed. Conclusion ACT001 significantly ameliorated inflammation and pyroptosis via the PPAR-γ/NF-κB signaling pathways in LPS-induced NR8383 alveolar macrophages.

Experimental analysis on the main contents of Rhizoma gastrodiae extract and inter-transformation throughout the fermentation process of Grifola frondosa

Na Wang,Tian-xiang Wu,Yong Zhang,Xiao-bao Xu,Sha Tan,Hong-wei Fu 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.3

Gastrodin (GA), p-hydroxylbenzaldehyde (HBA),p-hydroxybenzyl alcohol (gastrodigenin, HA) and parishin notonly are the major active ingredients of Rhizoma gastrodiae,but exist transformed relations from each other throughout thefermentation process of Grifola frondosa in this work.We hadfound that parishin (non-free gastrodin) almost could completelytransformed into gastrodin (GA, free gastrodin) afterR. gastrodiae alcohol extract was sterilized by moist heat(121 C, 30 min), but before was added into submerged cultivationof G. frondosa. However, interestingly and importantly,gastrodin re-synthesized of parishin after R. gastrodiaealcohol extract’s addition into submerged cultivation ofG. frondosa. In addition, the reduction of p-hydroxylbenzaldehydeand p-hydroxybenzyl alcohol in G. frondosa fermentationprocess reconfirmed that the G. frondosa strain 51616really could synthesize gastrodin into parishin by submergedfermentation. This paper firstly also reported G. frondosa’seffects on R. gastrodiae.

Hyperbilirubinemia Reduces the Streptozotocin-Induced Pancreatic Damage through Attenuating the Oxidative Stress in the Gunn Rat

Fu, Yan Yan,Kang, Kyung Ja,Ahn, Jung Myung,Kim, Hae-Ryoung,Na, Ki Young,Chae, Dong-Wan,Kim, Suhnggwon,Chin, Ho Jun Tohoku University Medical Press 2010 The Tohoku journal of experimental medicine Vol.222 No.4

<P>Oxidative stress is an important pathogenic factor in diabetes. Bilirubin may serve a cytoprotective function as an anti-oxidant. The Gunn rat lacks the enzyme uridine-diphosphate glucuronosyltransferase that is responsible for conjugation of bilirubin, exhibiting elevation of plasma bilirubin. We examined the effect of hyperbilirubinemia on the pancreatic damage caused by streptozotocin (STZ) in the Gunn rat. Male Wistar rats and male Gunn rats were treated with STZ (WS and GS groups, respectively) or vehicle (WC and GC groups, respectively). All 5 rats in the WS group developed diabetes, defined as fasting blood glucose 300 mg/dL or more, at 3 days, whereas only 2 of the 5 GS rats became diabetic at 7 days after STZ injection. Without insulin supplement at 7 days after STZ injection, the WS group displayed higher levels of fasting blood glucose (510.3 ± 50.3 vs. 236.4 ± 42.5 mg/dL, <I>p</I> = 0.003) and HbA1c (5.0 ± 0.1 vs. 3.9 ± 0.1, <I>p</I> = 0.001), compared to those of GS group. In Wistar rats, STZ induced apoptosis of the pancreatic islet cells, accompanied with activation of NADPH oxidase and increased production of reactive oxygen species and nitric oxide, but not in Gunn rats. Moreover, in a rat insulinoma cell line (RIN-m5F), pre-treatment with bilirubin (0.1 mg/dL) decreased cell death and apoptosis caused by STZ, and also reduced H<SUB>2</SUB>O<SUB>2</SUB> production. Considering the protective effect of hyperbilirubinemia against STZ-induced injury, we postulate that bilirubin could be a potential therapeutic modality for oxidative stress of pancreas islets.</P>

Channel Model and Throughput Analysis for LEO OFDM Satellite Communication System

Zhenyu Na,Qinyang Guan,Ce Fu,Yang Cui,Qing Guo 보안공학연구지원센터 2013 International Journal of Future Generation Communi Vol.6 No.6

Life Science : Chemical Constituents of Cynanchum wilfordii and the Chemotaxo-nomy of Two Species of the Family Asclepiadacease, C wilfordii and C auriculatum

( Yan Fu Jiang ),( Hyun Gyu Choi ),( Ying Li ),( Yu Mi Park ),( Jong Hwa Lee ),( Do Hoon Kim ),( Je Hyun Lee ),( Jong Keun Son ),( Min Kyun Na ),( Seung Ho Lee ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0

Definitive identification of original plant species is important for standardizing herbal medicine. Although only the dried roots of Cynanchum wilfordii (Asclepiadaceae) are prescribed as Cynanchi WilfordiiRadix in Korean Pharmacopoeia, the roots of C. wilfordii and C. auriculatum are often misused in the Korean herbal market due to their morphological similarity and similar name. Therefore, it would be very useful to discover an effective chemical marker for the identification of the two species. To this end, we carried out a phytochemical study on the roots of C. wilfordii. As a result, twenty compounds were isolated from the roots of C. wilfordii and their chemical structures were identified as β-sitosterol (1), wilfoside C1N (2), wilfoside C3N (3), wilfoside K1N (4), methyleugenol (5), wilfoside C1G (6), cynauriculoside A (7), daucosterol (8), 2,4-dihydroxyacetophenone (9), cynandione A (10), 2,5-dihydroxyacetophenone (11), acetovanillone (12), p-hydroxyacetophenone (13), sucrose (14), conduritol F (15), geniposide (16), succinic acid (17), 3-(β-D-ribofuranosyl)-2,3-dihydro-6H-1,3-oxazine-2,6-dione (18), bungeiside A (19), cynanoneside B (20). Among them, compounds 15, 16, 18, 19, and 20 were isolated for the first time from this species. Furthermore, conduritol F (15) was demonstrated to be contained only in C. wilfordii. Therefore, it may be useful as a chemical marker to identify the two species C. wilfordii and C. auriculatum.

Isolation and Characterization of Bioactive Compounds from Root of Rubus coreanus Miquel and their Antimicrobial Activity

Jang, Ha Na,Ha, Ji Hoon,Lee, Yoon Ju,Fu, Min Min,Park, Soo Nam The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.1

Rubus coreanus Miquel (RCM), also known as Korean blackberry or bokbunja, is used as a South Korean traditional medicine to treat acne and inflammatory skin conditions. The antimicrobial activity of RCM root and its active compounds remain unclear. In this study, we prepared a 50% ethanol fraction, ethyl acetate fraction, and acid-treated ethyl acetate fraction (aglycone fraction) of RCM root, and evaluated antibacterial activities against the skin pathogens Staphylococcus aureus, Pseudomonas acnes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. In a paper disc assay, all fractions of RCM root showed antimicrobial activities against the five skin pathogens. The ethyl acetate fraction displayed 6-, 12-, and 2-fold higher minimal inhibitory concentration (MIC) than the 50% ethanol fraction against S. aureus, E. coli, and P. acnes, respectively. The aglycone fraction displayed 2-fold higher MIC than methyl paraben against P. acnes, S. aureus, E. coli, and P. aeruginosa. The ethyl acetate fraction displayed a minimal bactericidal concentration (MBC) similar to that of methyl paraben, and the aglycone fraction showed 2- to 4-fold higher MBCs than those of methyl paraben. In particular, the ethyl acetate fraction was not cytotoxic and showed thermal stability after incubation at high temperatures ($60-121^{\circ}C$). Finally, the ethyl acetate fraction was separated and four components were identified: procyanidin C, propelagonidin dimer, ellagic acid, and methyl ellagic acid acetyl pentose. The compounds showed high antibacterial activities. These results suggest that RCM root is potentially applicable as a natural preservative in cosmetics.

A Driver Circuit of Spatial Light Modulator

Lan Wu,Fu-na Zhou,Minghao Zhu 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.6