골수암유래 암세포주 KHOS/NP와 MG-63의 성장에 미치는 마늘의 암세포 독성효과

이정석,임성남,오명숙,지은정 全北大學校 齒醫學硏究所 1993 전북치대논문집 Vol.11 No.1

Most anticancer drugs so far used have brought many side effects to the host, which is inevitable since they can not specifically distinguish between cancer cells and rapidly dividing normal cells. Therefore, it is very important to develope a chemopreventive agent proven for a long period of time not to toxic to normal cells. Since garlic has been attracted interests from many areas of cancer researchers as a potent anticancer agent, attempts were made to see the direct cytotoxic effect of garlic to human osteosarcoma cell lines. The present study was designed to investigate the effect of garlic on the two cell lines of rapidly growing osteosarcoma with comparable doubling time of 20 hours, namely KHOS/NP and MG-63. Four different garlic preparations commnly used in the Koreans, that is, raw and heated garlic dissolved in distilled water, raw and heated garlic dissolved in saline were compared in thier cytotoxic effect to the KHOS/NP and MG-63. The results obtained were as follows. 1. Raw garlic dissoved in distilled water showed the best cytotoxic effect among four different preparations of garlic used in this study. 2. The cytotoxic effect of garlic to the osteosarcoma was different, to the cell lines showing mostly more effective to KHOS/NP than to MG-63. 3. To KHOS/NP, the cytotoxic effect of garlic was markedly increased with concentrations but not with incubated time. 4. To MG-63, the cytotoxic effect of garlic w3s increased both with concentrations and incubated time. 5. The difference of inhibitory action of garlic between KHOS/NP and MG-63 was marked when heated garlic in saline was treated at high concentration(0.1875g% ) for 48 hours.

초고속 통신망에서 비디오의 주관적 품질 평가에 관한 연구

강명화,강진석,최연성,김장형 제주대학교 산업기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.1

According to information communication business faces with competitions in a various spheres with the development of multimedia and information communication, the time when we have to make an effort to improve a quality comes from an era when we devote ourselves to quantitative expansion. we will try to perform quality assessments of real-time interactive multimedia service, and so on - in this research and present criteria of new subjective quality assessments which is fit on Korea by analyzing examples of ITU-T and foreign countries and Korea.

혈소판 농축 혈장이 임플란트 주변 골형성에 미치는 효과에 대한 연구

백명환,한석환,권종진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.1

Various methods have been used to fill in the defects around the implants. Recently, researchs of developing and purifying various growth factors, and efforts to apply these growth factors in clinical situations have been carried out. Among those tries, platelet-rich plasma(PRP) containing abundant PDGF and TGF-β obtained by the sequestration and concentration of platelets by the gradient density centrifugation became one of the popular and preferred methods becuase of relatively simple procedure and abundant growth factor components. Bone morphogenic protein(BMP), platelet-derived growth factor(PDGF), and transforming growth factor-beta(TGF-β) are growth factors in the PRP that promote the bone formation. To elucidate the effects of PRP on the bone formation in peri-implant bone defects, twenty-seven Steri-Oss implants were inserted in the mandibular premolar areas of 8 adult dogs and artificial bone defects(3×4×4㎜) were prepared at the buccal side of the implants in 1st premolar, at the distal side of the implants in 3rd premolar. The control groups were filled with bovine bone grafts without PRP, and the experimental groups were filled with bovine bone grafts with PRP. Animals were sacrificed at 1, 2, 4, 8, and 12 weeks after the operation and speimens were observed by light and electron microscope. The results were as follows. first, the bone formation was observed at 1st week in the experimental groups, but at 2nd week in the control groups. second, the mature bone was observed at 4th week in the experimental groups. but 8th week in the control groups. the bone growth rate of experimental groups was more rapid than that of control groups. third, at 12th week, bone density ratio was higher in the experimental groups(71.65%) than that of the control groups(48.49%). fourth, Grafted bovine bone were almost completely absorbed at 1st week in experimental groups, but it was still present at 8th week in control groups. From the above results, PRP may accelerate the formation of new bone by promoting absorption of the graft material and increasing the growth rate of bone.

기(氣)수련이 스트레스 반응에 미치는 영향

박미숙,이명수,정영자,김혜정,문성록,김용규 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.3

본 연구는 스트레스 반응을 측정하는 스트레스 반응 척도 설문지(Symptoms of Stress: SOS)를 이용한 횡단적 연구로서 심신수련의 하나인 기수련이 스트레스 반응에 미치는 효과를 살펴보았다. 72명의 건강한 정상인과 180명의 기수련자를 대상으로 하여 조사하였으며, 기수련자들은 수련기간에 따라 초급군(1-4개월; 64명), 중급군(5-12개월; 50명), 고급군(1년 이상; 66명)로 나누었다. 실험결과 1년 이상 수련한 기수련자들이 말초혈관 증상군, 심폐 증상군, 중추신경계 증상군, 위장계증상군, 습관적 행동 형태군, 우울 증상군, 불안증상군등의 스트레스 반응 하위척도에서 대조군에 비해 통계적으로 유의하게 감소하였다. 전체 스트레스 값은 대조군에 비해 기수련자 집단이 아주 낮았으며 수련기간이 길수록 감소하였다. 이러한 결과는 기수련이 스트레스 중재와 스트레스 증상을 줄이는데 효과가 있음을 나타낸다. We examined the effects of Qi-training, one of Korean traditional psychosomatic training, on stress coping ability by cross-sectional investigation with inventory of symptoms of stress (SOS). Four types of groups- normal healthy (n-74), three groups of Qi-trainees (group primary level: subjects with Qi-training for 1-4months, n=64; middle level: 5-12 months, n=50; high level: more than 12 months, n=66) participated in this study, Qi-trainees with over 1 year training had significantly lower scores in all SOS subscales compared to controls. Total stress scores of Qi-trainee groups were substantially lower than controls and further reduction with larger training periods. These results suggest Qi-training is effective in stress management and decreasing symptoms of stress

유산균 투여가 건강한 성인의 분변미생물 및 부패산물 생성에 미치는 영향

신명수,김용재,배형석,백영진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

유산균의 장내균총과 부패산물 생성에 미치는 영향을 알아보기 위하여, 건강한 성인 남녀 6명의 지원자에게 Lactobacillus acidophilus 와Bifidobacterium longum으로 제조한 유산균분말(각각 1.5×10 exp (9) cells)을 하루에 2회씩 2주동안 섭취하도록 하였다. 그리고 지원자 분변으로부터 균총수 변화와 부패산물인 암모니아, indole, skatole, p-cresole량을 측정하였다. 유산균의 투여 기간중에 분변내 bifidobacteria의 수가 8.78±0.39(log cfu/g feces±S.D)에서 9.27±0.29로 증가하였고(p<0.05) 투여 중지후에는 약간 감소하였으며, lactobacilli의 수는 6.15±0.80에서 6.76±0.48로 증가하였다(p<0.05). Enterococcus 균수는 투여 기간중에 6.66±0.80에서 7.72±0.40으로 증가하였다가 투여 중지후에는 감소하는 경향을 보였다. 그리고 혐기성균인 Bacteroides는 투여중에 8.45±0.34에서 9.15±0.21으로 증가하였으나(p<0.01), 유해균주로 알려진 Closridium(lecithinase negative)과 Staphylococcus 균수는 현저히 감소하였다. 그러나, 유산균 투여 실험기간인 6주 동안 분변내 암모니아, indole, skatole, p-cresole의 유의적인 농도변화는 없었다. To investigate the effects of lactic acid bacteria administration on fecal microflora and putrefactive metabolites in human being, Lactobacillus acidophilus and Bifidobacterium longum powder (1.5×10 exp (9) cells, respectively) was administrated to six healthy volunteers (average 28 years old) twice a day for 2 weeks. During the administration of lactic acid bacteria, the numbers of bifidobacteria, lactobacilli, and enterococci in feces were increased significantly, whereas those of Staphylococcus and lecithinase-negative Clostridium were decreased considerably. In addition, a number of anaerobic Bacteroides were increased. However, the contents of fecal ammonia and putrefactive metabolites (indole, skatole, p-cresole) were not changed during the administration.

꿀벌의 日週收蜜活動에 關與하는 諸要因에 關한 硏究

李明熱,吳賢宇,崔承允,禹建錫 서울대학교농과대학농업개발연구소 1989 서울대농학연구지 Vol.14 No.1

In order to employ the honeybees (Apis mellifera) profitably as insect-pollinators, the factors governing their diurnal foraging activity, the patterns of diurnal flight activity at the hive entrance and the seasonal fluctuation of colony size were examined. The results obtained are summarized as follows. 1. There was a maximum flight activity at the hive entrance between 11h and 15h during the spring and fall but it was between 08h and 09h or 16h and 17h during the summer. This activity highly correlated with the solar energy(Mj/m²) during the spring and fall. 2. The brood area and colony weight developed to the maximum in early July but total number of flying bees at the hive entrance reached the peak early in May, and decreased in June but increased again late in July. 3. The higher the degree of flowering developed, the more active the foraging behavior. Though the degree of flowering was similar in different varieties, the foraging preference in the apple blossoms differed with varieties. 4. The amount of nectar per flower was variable with time hours, age of flowers and plant varieties. 5. The number of honeybees collecting only the nectar in the apple blossoms increased in the afternoon. Foraging behavior in the pear blossoms changed with the varieties and the numbers of honeybees collecting only the pollen (66%) or only the nectar (31%) did not change with time in the peach blossoms. 6. The diurnal foraging activities of honeybees on eight honey plants highly correlated with the solar energy but temperature, relative humidity and wind speed were not.

湖南地方의 高等學生과 大學生들의 結婚觀

李明淑 朝鮮大學校 1977 綜合論文集 Vol.1977 No.-

We know that marriage is very important matter for our life, but the effort to develop a marriage by scientific method is very weak so far. In Korea, the study on the marriage has been carried out partially, so the views of marriage for young people has come into question in our society which has been influenced by the countries in Europe and America these days. In this research, it is the purpose to give the right views of marriage for young people through the statistical comparative study on the views of marriage between high school students and college students. The difference between high school students and college students by the result of study is as follows: 1. In a way to get married, both high school students and college students want a compromising way of high stability through half free love and half matchmaker. For the age of women's marriage the views of high school students is one year younger than the views of college students. 2. In academic career of a spouse, college students want more higher one than high school students. Under the condition of choice of a spouse, college students are interested in spiritual matter, but high school students are more interested in material comport and physically strong. 3. In a way to select a spouse, high school students want to find one by, themselves, but college students want to find one through their parents or their friends. 4. Both high school students and college students do not want to live together with their parents after get married. In economical aspect, high school students want more women's job after get married than college students. 5. Both high school students and college students think that to make a love and to get married are different thing. In the purity problem, college students throw open more than high school students. 6. For the ideal coiple both high school students and college students want marriage life with friendship.

ICT를 활용한 수학과 교수-학습 방안 연구

진명숙,정권수,엄경련 순천대학교 과학교육연구소 2001 科學과 敎育 Vol.9 No.-

The purpose of thesis is to make the students who are not interested in athematics have interest in mathematics by using information & Communication Technology(ICT), to understand the nathematical principles, to know how close they are to us through data connected with real life and direct manufacturing and experiment of cyberspace, to solve the mathematics for themselves, and to make mathematics teachers develop and make use of ICT teaching-learning manual. Let’s see the following thesis for this purpose. First, it was divided into 8 teaching-learning activity types in connection with characteristics and educational use possibility of Information Technology. It was analyzed into concept, teaching-learning process procedures, and illustration for teaching-learning. Second, gathered and analyzed the scattered internet sites about mathematics, and made use of the intenet sites for teaching and learning mathematics. Third, for effective ICT teaching-learning. I chose the teaching-learning main idea, objectives and teaching-learning activity type. and I checked the aility to use information Technology and then chose the environment and media for information Technology. And then I planned the teaching-learning strategy. Fourth, I developed and applied the teaching-learning model using information Technology, and analyzed the results. This ICT teaching-learning activities brought interests for mathematics, accomplishment motivation, confidence about mathematics, and sense of superiority to the students. In addition to my student became active in mathematics class through this ICT teaching-learning activities.

McCoy 세포에서 Chlamydia trachomatis의 병원성 인자에 관한 연구

강정숙,최지영,차영주,김영주,김덕례,여명구,박열 조선대학교 생명과학연구소 1998 생명과학 연구 Vol.6 No.-

본 연구는 병원성 세균인 Chlamydia trachomatis(C. trachomatis)가 숙주세포에침습시 C. trachomatis의 병원성 인자와 C. trachomatis와 상호작용하는 숙주 세포막의 인자를 규명함으로써 숙주세포에 대한 감염율을 향상시켜 보다 향상된 진단 방법을 개발하기 위하여 수행하였다. C. trachomatis로 감염된 세포에서 기본소체의 막 단백질을 분리한 결과 각 혈청형의 39 kDa과 42 kDa에서 major outer membranes(MOMPs)이 관찰되었으며, 혈청형 E와 G의 MOMPs는 42.5 kDa으로 동일하였지만 혈청형 F, H, I, 그리고 K의 MOMPs는 각각 다르게 관찰되었다. C. trachomatis가 숙주세포로 침습시 관련된 병원성 인자에 대하여 조사하고자 단층세포로 배양된 배양용기에 C. trachomatis를 24, 48 그리고 73시간대별로 접종시켜 관찰한 결과 72시간의 MOMPs의 양이 가장 많이 증가하였으며 이 결과로 MOMPS이 C. trachomatis 병원성에 중요한 작용을 함을 확인할 수 있었다. C. trachomatis가 침습시 숙주세포의 막 단백질 변화양상을 관찰하기 위해 정상세포와 C. trachomatis로 1, 2, 3 그리고 4시간대별로 접종 시간을 달리한 숙주세포의 막 단백질을 분리한 결과 42 kDa의 단백질 양이 Chlamydia에 감염된 세포 내에서 증가됨을 관찰할 수 있었다. 이로 42 kDa의 막 단백질이 침습시 숙주세포 표면에 대한 수용체로서 작용함을 알 수 있었다. The present study was performed to analyze the pathogenic factors involved in the invasion process of Chlamydia trachomatis(C. trachomatis) into McCoy cells. In this study, elementary bodies(EBs) of C. trachomatis serotypes(E, F, G, H, I, K and LGV) could be isolated by the method of Percoll density gradient centrifugation and the pathogens of serotypes were also compared from whole-cell lysates by using sodium dodecyl sulfate-polyacrylamide gel elelectrophoresis. All chlamydial serotypes showed a single predominant protein ranging from 39 to 42 kDa. The major outer membrane proteins(MOMPs) of the E and G serotypes had an identical molecular weight of 42.5 kDa. In contrast, the MOMPs of the F, H, I and K serotypes were showed different molecular weights of MOMPs. By immunoblotting with anti-MOMP antibody it was revealed that the quantify of MOMPs was time-dependently increased when McCoy cells were infected with Chlamydia for 24, 48 and 72 hr. These results indicated that MOMPs are closely related to chlamydial infection into the host cell.

수용성 Polymer-metal Complex의 형성과 중합 개시제로서의 활용성에 관한 연구(Ⅰ)

맹기석,송해영,권오승,황명천,황택성 충남대학교 공업교육연구소 1983 論文集 Vol.6 No.2(B)

The formation of Water-Soluble Poly(Methacrylic acid) (PMA) and poly (acrylamide) (PAAm)-Cu(II) complexes was achieved at various PH. The structures and the optimum conditions for the formation of complexes have been determined through UV, IR and viscosity measurements. In addition, it was found that they can be used as an initiator for vinyl type monomers. Formed polymer-metal complexes are the most stable at around PH=7 and therefore give rise to maximum absorbance in UV spectrum. As PH is increased the conformation of polymer-metal complexes has become more compact. This can be explained in terms of decreasing viscosity with increasing PH. It was also observed that the function as an initiator of the polymer-metal complexes is very active at around PH=7 and the complexes can polymerize vinyl type monomers up to 10% Conversion. These results can explain the fact that the stable complexes were formed at around PH=7 and the formed complexes can be used as good initiators.