중증 외상환자에서 염기 결핍수치의 유용성

문준동,김수진,문철규,최성혁,전정민,이성우,홍윤식 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: This study's objective was to determine the prognostic value of the base deficit measured in the emergency department(ED) and to determine whether base deficit can provide information not provided by advanced injury scoring system. Methods: This study was a retrospective analysis of data collected for two years. Thirty-two severe trauma patients who were admitted to the Emergency Department of Korea University Hospital were included in this study. The patients were divided into two groups: the normal base deficit group(-3 mmol/L to 3 mmol/L) and the elevated base deficit group(>3 mmol/L). The base-deficit value, age, sex, head injury, organ failure rate, and survival were considered, and the also Revised Trauma Score(RTS), Acute Physiology And Chronic Health Evaluation(APACHE II), and Injury Severity Score(ISS) were measured. Result: The elevated base-deficit group showed a lower survival rate and a higher organ failure rate compared to the normal base deficit group. Logistic Regression showed a strong association between base deficit and mortality rate. Among the previous injury scoring Systems(RTS, APACHE II, ISS), base deficit had the strongest correlation with RTS. By using base deficit value and the RTS together, we obtained a higher positive predictive value than that obtained by using base deficit or RTS alone. Conclusion: The admission value of the base deficit in the ED is a useful tool in predicting the outcome in severe trauma patients, and it can be an adjunct to previous injury scoring systems. As an advanced injury scoring System is developed in the future, the base deficit may have some significant role.

결절성 및 괴상성 간세포암에서 역동적 전산화단층촬영 소견에 따른 경동맥화학색전술결과의 예측

이성문,김홍,지성우,우성구,이정형,서수지,손철호 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.2

Purpose: To evaluate retrospectively whether it is possible to predict the transarterial chemoembolization(TACE) result with the pre-TACE dynamic CT findings. Materials and Methods: The dynamic CT findings of 33 patients with 35 nodular and 8 massive hepatocellular carcinoma(HCCs) who was taken follow up CT after TACE, were reviewed retrospectively. After excluding the diffuse or infiltrative HCCs, the mass was classified into two groups of nodular and massive types. The size, location, degree of enhancement, pseudocapsule, portal vein(PV) thrombosis and arterioportal(AP) shunt were evaluated on the dynamic CT scans. The vascularity, multiple or parasitic blood supply, AP shunt and the possibility of superselection were considered on the angiography. After TACE, the pattern of lipiodol uptake and residual enhancing nodules were evaluated on the follow-up CT scans. Results: The good TACE results could be obtained in 18 of 35 nodular HCCs with dynamic CT findings of small size(mean=2.94cm), peripheral location, hyper-or iso-enhancement, and absence fo PV thrombosis, with angiographic finding of possible superselection, but the pseudocapsule did not give important effect on the result. Most of the massive HCCs had poor TACE results. Conclusion: The prediction of the TACE results of nodular and massive HCCs was possible with dynamic CT findings. If the good prognostic factors were not observed on the pre-TACE dynamic CT, the multiple TACE and/or combination therapy with percutaneous ethanol injection(PEI), surgical resection or others should be considered for the effective control of the HCCs.

이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향

황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Inheritance and linkage mapping of resistance genes to insects, stink bug (Riptortus clavatus) and adzuki bean weevil (Callosobruchus chinensis)

Jung Kyung Moon,Young Sun Kim,Jin Kyo Jung,Ja Hwan Ku,Young Tae Kim,Jeong Bong Kim,Hong Tae Yun,Yeong Ho Lee,Si Ju Kim 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.1

A New Soybean Cultivar with Lodging Resistance and Adaptability for Mechanized Harvesting, "Shingi"

Jung Kyung Moon,Keum Yong Park,Hong Tae Yun,Yeong Ho Lee,Min Jung Seo,Sun Lim Kim,Yong Hwan Ryu,Suk Ha Lee,Ja Hwan Ku,Jae Hwan Roh,Jung Soo Park,Ik Jae Kim,Soo Kyeong KimMoon 한국육종학회 2005 한국육종학회지 Vol.37 No.2

Protective Effects of Fucoidan against UVB-Induced Oxidative Stress in Human Skin Fibroblasts

Mi Jung Ku(구미정),Myeong Sook Lee(이명숙),Hee Jung Moon(문희정),Yong Hwan Lee(이용환) 한국생명과학회 2010 생명과학회지 Vol.20 No.1

자외선 B는 반응성 산소종(reactive oxygen species, ROS)의 발생을 유도하고 세포내 항산화 물질의 저장을 방해한다. 후코이단은 L-fucose와 황 에스터기를 포함하는 다당류로서 갈조류의 주요 성분으로 알려져 있다. 본 연구에서는 인간 피부 섬유아세포인 HS68세포에 자외선을 조사하여 산화 스트레스를 유도한 후 후코이단의 보호효과를 확인하고자 하였다. 후코이단의 전처치로 malondialdehyde (MDA)의 양이 용량-의존적으로 감소하였고, 세포내 glutathiopne도 후코이단 100 ㎍/ml을 투여하였을 때 후코이단을 투여하지 않고 자외선만 조사한 군에 비하여 21.5% 감소하였다. 후코이단을 10, 100 ㎍/ml의 용량으로 투여하였을 경우 자외선 B에 의한 ROS 생성이 자외선만 조사하였을 때 보다 각각 40.1%와 68.4%로 유의하게 감소하였다. 세포노화와 관련된 β-galactosidase의 양성 염색률은 후코이단을 10, 100 ㎍/ml 투여하였을 경우 자외선만 조사하였을 때보다 각각 23.1%, 16.4% 감소하였다. 이러한 결과로 미루어 후코이단은 자외선에 의한 산화 스트레스에 대하여 광보호 효과가 있는 것으로 생각된다. Ultraviolet-B (UVB) radiation induces the formation of reactive oxygen species (ROS) and depletes stores of cellular antioxidants. Fucoidan, polysaccharides containing L-fucose and sulfate ester groups, are constituents of brown algae. In this study, the protective effects of fucoidan on UVB-induced oxidative stress in cultured human skin fibroblast HS68 cells were assessed. Pretreatment with fucoidan significantly reduced malondialdehyde (MDA) content in a dose-dependent manner. With fucoidan pretreatment at a dose of 100 ㎍/ml, the level of intracellular glutathione was increased by 21.5%, compared to UVB irradiation alone. Fucoidan significantly reduced UVB-induced ROS generation by 40.1% and 68.4% at 10 and 100 ㎍/ml, respectively, compared to UVB irradiation alone. The positive staining rates of senescence-associated β-galactosidase were reduced by 23.1% and 16.4% with 10 and 100 ㎍/ml of fucoidan, compared to UVB irradiation alone. Fucoidan may exert a photoprotective effect against UVB radiation-induced oxidative stress.

Survivorship of the bean bug, Riptortus clavatus, fed on seed extract fractions of amungbean variety

Jin Kyo Jung,Bo-Yoon Seo,Jung-Kyung Moon,Ja-Hwan Ku 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

To isolate plant insecticidal factors, a series of survivorship tests were conducted using artificial diets mixed with seed extract of a mungbean variety (Vigna radiata), which gave a high mortality to the bean bug, Riptortus clavatus. Seed flour was extracted with methanol and ethanol solutions, and then the crude extract was first fractionated with layers of ether, n-butanol and water in solvent/solvent partition. Layers of ether and n-butanol fractions showed insecticidal effects to the bean bug, respectively. The n-butanol fraction was further isolated with several fractions on a reversed-phase column chromatography, and its 100% methanol fraction showed a high insecticidal effect. The fraction was further isolated on a normal-phase column chromatography, and the 70% methanol fraction showed the highest insecticidal effect. The final fraction was analyzed and fractionated on a reversed-phase HPLC. The active isolates were almost insoluble in any organic solvents and did not show any absorption in ultra-violet and visible light wavelength range. On the other hand, the ether fraction of crude extract was isolated on a normal phase column chromatography, and its hexane fraction showed the activity. The hexane fraction was further isolated on a reversed-phase HPLC and tested for the survivorship of the bean bug.

원인 불명의 위장관 출혈에서 이중 풍선 소장내시경의 유용성

정현구 ( Hyun Ku Jung ),위준환 ( Jun Hwan Wi ),김진오 ( Jin Oh Kim ),정인섭 ( In Seop Jung ),고봉민 ( Bong Min Ko ),조주영 ( Joo Young Cho ),이준성 ( Joon Seong Lee ),이문성 ( Moon Sung Lee ),심찬섭 ( Chan Sup Shim ),김부성 ( Boo 대한내과학회 2007 대한내과학회지 Vol.73 No.3

목적: 위내시경 및 대장내시경검사에서 출혈부위를 확인할 수 없는 원인 불명의 위장관 출혈 환자에 있어서 소장검사는 필수이며 현재 캡슐내시경과 소장내시경의 발달로 소장 병변을 진단하는데 유용하게 쓰이고 있다. 이번 연구는 원인 불명의 위장관 출혈 환자에게 이중 풍선 소장내시경을 시술하여 그 유용성을 평가하고자 시행하였다. 방법: 2004년 11월부터 2005년 8월까지 순천향대학교병원 소화기병센터에서 혈변, 흑색변과 토혈로 위내시경 및 대장내시경 검사를 시행하였으나 출혈 병소를 찾지 못했던 24명의 환자를 대상으로 이중 풍선 소장내시경 검사를 시행하였다. 결과: (1) 대상 환자 24명의 평균연령은 48세(범위 21-81세)였고, 남자는14명, 여자 10명이었으며 내원 당시 환자의 평균혈색소(Hb)는 9.0 g/dL (범위 5.9-11.9 g/dL)이었다. (2) 이중 풍선 소장내시경 검사의 접근방법으로는 구강 7예, 항문 3예, 구강, 항문 양쪽 모두에서 접근한 경우가 14예에서 시행이 되었으며 검사에 관련된 합병증은 발생되지 않았다. (3) 24명의 환자 중 22명에서 출혈 병소가 관찰되었으며 출혈 원인으로는 소장궤양 9예, 혈관이형성증 6예, 크론병이 3예, 위장관 간질종양 2예, 메켈 게실 1예, 소장 외 병소 1예가 관찰되었다. (4) 출혈 병소의 위치는 근위부 공장이 가장 흔하였으며 2곳 이상의 병변이 10예에서 관찰되었다. 결론: 이중 풍선 소장내시경 검사는 기술을 요하는 검사이기는 하나 안전한 검사이며 소장의 병변은 물론 특히 원인 불명의 위장관 출혈에서 출혈 병소를 찾는데 유용한 검사로 생각한다. Background: Double balloon enteroscopy was developed to improve access to the small intestine. The aim of this study was to evaluate the efficacy of double balloon enteroscopy in patients with obscure gastrointestinal bleeding. Methods: From November 2004 through August 2005, 24 consecutuve patients (14 males, 10 females; mean age 48±15.1 years, range 2181 years) with gastrointestinal bleeding of an obscure origin were enrolled in this study. The patients underwent enteroscopy using the double balloon technique for the following indications: (1) clinical evidence of gastrointestinal bleeding such as melena and hematochezia (the Hb levels ranged from 5.9 g/dL to 11.9 g/dL, mean 9.0±2.3 g/dL) (2) no site and cause of blood loss detected by upper endoscopy and colonoscopy. Results: Of 24 patients that underwent a double balloon enteroscopy, bleeding points were identified in 22 patients. The causes of bleeding were nine small bowel ulcera, six angiodysplasiaa, three cases of Crohn`s disease, two gastrointestinal stromal tumors, one Meckel`s diverticulum and one cecal diverticular ulcer. However, two cases showed negative findings. No patient suffered from procedure related complication. Conclusions: Double balloon enteroscopy is a safe and useful diagnostic tool for obscure gastrointestinal bleeding. (Korean J Med 73:267-273, 2007)

Fucoidan의 멜라닌 합성과 tyrosinase 활성도 억제 효과

정숙희(Sook Hee Jung),구미정(Mi Jung Ku),문희정(Hee Jung Moon),유병철(Byeng Chul Yu),전만중(Man Joong Jeon),이용환(Yong Hwan Lee) 한국생명과학회 2009 생명과학회지 Vol.19 No.1

미역, 다시마 등 갈조류의 추출물로서 생리활성성분으로 각광 받고 있는 fucoidan이 멜라닌 합성에 미치는 영향을 알아봄으로써 미백제로서의 개발 가능성 여부를 확인하기 위하여 B16F10 melnoma 세포를 이용하여 멜라닌 합성과 tyrosinase 활성도에 대한 실험의 결과 B16F10 melnoma 세포의 멜라닌 생성과 tyrosinase 활성도는 fucoidan의 농도가 증가함에 따라 농도 의존적으로 억제되었다. B16F10 melnoma 세포에 α-MSH를 투여 후 멜라닌 생성과 tyrosinase의 활성도 역시 fucoidan의 농도가 증가할수록 멜라닌 생성과 tyrosinase활성도가 억제되는 경향을 보였다. 또한 DOPA 염색의 결과 fucoidan은 농도 의존적으로 tyrosinase 활성도를 억제하였다. 이상의 결과 해조 추출 다당류인 fucoidan은 B16F10 melanoma 세포의 멜라닌 합성과 tyrosinase 활성도 억제 작용을 나타내므로 미백 활성 물질로서의 가능성을 가지고 있는 것으로 생각된다. Melanogenesis is a physiological process that results in the synthesis of melanin pigments. Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. Among the possible melanin-reducing compounds, tyrosinase inhibitors are most promising for preventing and treating pigmentation disorder and are used as skin-whitening agents in the cosmetic industry. In the present study, the effects of fucoidan on melanogenesis and tyrosinase activity of B16F10 melanoma cells were investigated. Melanin synthesis and tyrosinase activity in B16F10 melanoma cells were decreased in a dose-dependent manner by fucoidan. Melanin production and tyrosinase activity in B16F10 melanoma cells stimulated by α-melanocyte stimulating hormone (α-MSH) were inhibited by fucoidan with a dose-dependent manner compared to control. Fucoidan inhibited tyrosinase activity of B16F10 melanoma cells with a dose-dependent manner as assessed by 3,4-dihydroxyphenylalanine (DOPA) staining. In conclusion, these findings indicate that fucoidan, which inhibit melanin synthesis and tyrosinase activity, is an effective skin-whitening agent.