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Kim, Joonki,Jung, Jae-Hoon,Reyes, Jose L.,Kim, Youn-Sung,Kim, Sun-Young,Chung, Kyung-Sook,Kim, Jin A.,Lee, Minsun,Lee, Yoontae,Narry Kim, V.,Chua, Nam-Hai,Park, Chung-Mo Wiley (Blackwell Publishing) 2005 The Plant journal Vol.42 No.1
<P>Class III homeodomain-leucine zipper proteins regulate critical aspects of plant development, including lateral organ polarity, apical and lateral meristem formation, and vascular development. ATHB15, a member of this transcription factor family, is exclusively expressed in vascular tissues. Recently, a microRNA (miRNA) binding sequence has been identified in ATHB15 mRNA, suggesting that a molecular mechanism governed by miRNA binding may direct vascular development through ATHB15. Here, we show that miR166-mediated ATHB15 mRNA cleavage is a principal mechanism for the regulation of vascular development. In a gain-of-function MIR166a mutant, the decreased transcript level of ATHB15 was accompanied by an altered vascular system with expanded xylem tissue and interfascicular region, indicative of accelerated vascular cell differentiation from cambial/procambial cells. A similar phenotype was observed in Arabidopsis plants with reduced ATHB15 expression but reversed in transgenic plants overexpressing an miR166-resistant ATHB15. ATHB15 mRNA cleavage occurred in standard wheat germ extracts and in Arabidopsis and was mediated by miR166 in Nicotiana benthamiana cells. miR166-assisted ATHB15 repression is likely to be a conserved mechanism that regulates vascular development in all vascular plants.</P>
Photocatalytic enhancement of cesium removal by Prussian blue-deposited TiO<sub>2</sub>
Kim, Hyuncheol,Kim, Minsun,Kim, Wooyul,Lee, Wanno,Kim, Soonhyun Elsevier 2018 Journal of hazardous materials Vol.357 No.-
<P><B>Abstract</B></P> <P>After the Fukushima nuclear accident, tremendous efforts were made to treat radiocesium, radiostrontium, and other radioactive materials. For the first time, we demonstrate that a TiO<SUB>2</SUB> photocatalyst can significantly enhance Cs adsorption by Prussian blue-deposited TiO<SUB>2</SUB> (PB/TiO<SUB>2</SUB>) under UV irradiation. In this study, we synthesized PB/TiO<SUB>2</SUB> using the photodeposition method. After the Cs ions were adsorbed on the PB/TiO<SUB>2</SUB> in darkness, we then exposed the PB/TiO<SUB>2</SUB> to UV light irradiation. This resulted in a further increase in Cs ion adsorption of more than 10 times the amount adsorbed in darkness. This photocatalytic-enhanced adsorption of Cs ions was not observed on PB mixed with SiO<SUB>2</SUB>, nor under visible light irradiation. We investigated the effects of PB concentration, PB/TiO<SUB>2</SUB> concentration, and gas purging on both dark and photocatalytic-enhanced adsorption of Cs ions by PB/TiO<SUB>2</SUB>. Based on the results, we suggest that the photocatalytic-enhanced adsorption of Cs ions on PB/TiO<SUB>2</SUB> is due to photocatalytic reduction of PB, which leads to additional adsorption of Cs ions. The change in solution color before and after the reaction, and the change in solution pH in the dark and during UV irradiation strongly support this suggestion. The photocatalytic-enhanced adsorption of Cs ions was equivalent during radioactive <SUP>137</SUP>Cs removal, indicating important applications for pollutant removal from contaminated water.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Prussian blue (PB)-deposited TiO<SUB>2</SUB> was synthesized using the photodeposition method. </LI> <LI> TiO<SUB>2</SUB> photocatalysts greatly enhance Cs adsorption by PB/TiO<SUB>2</SUB> under UV irradiation. </LI> <LI> Photocatalytic-enhanced Cs adsorption is due to photocatalytic reduction of PB. </LI> <LI> Photocatalysts can be used for radioactive <SUP>137</SUP>Cs removal from contaminated water. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Minsun,Shin, Seung Won,Lim, Cheol Won,Kim, Jaeyun,Um, Soong Ho,Kim, Dukjoon RSC Publications 2017 Biomaterials Science Vol.5 No.2
<P>Iron oxide nanoparticles (NPs) were encapsulated with polyaspartamide-based graft copolymers to bind and track the immune cells as imaging probes. Mono-disperse iron oxide NPs with a mean diameter of 10.7 nm were synthesized by the thermal decomposition method, and their shape and distribution were measured by electrophoretic light scattering and transmission electron microscopy. To enhance their biocompatibility, interfacial and hydrodynamic stability, and fluorescence detection, biodegradable polysuccinimide (PSI) grafted with several functional groups of octadecylamine (C18), ethanolamine (EA), ethylenediamine (EDA), 4-(<I>N</I>-maleimidomethyl) cyclohexane carboxylic acid<I>N</I>-hydroxysuccinimide ester (SMCC), and fluorescein isothiocyanate (FITC) was coated on the iron oxide NPs. The structure of the C18/EA/SMCC/FITC-<I>g</I>-PSI copolymer was confirmed using<SUP>1</SUP>H-NMR and FTIR spectroscopy, and its cell binding ability was investigated by flow cytometry and confocal laser scanning microscopy. The synthesized C18/EA/SMCC/FITC-<I>g</I>-PSI copolymer showed an excellent binding affinity to CD4+ T cells, and was highly biocompatible as the cell viability at the highest polymer concentration of 0.4 mg mL<SUP>−1</SUP>was greater than 85 and 75% after 24 and 48 h, respectively, from MTT assay.</P>
Camouflage Pattern Attributes According to Background Context
( Minsun Kim ),( Jaeyeong Ji ),( Yongjae Kim ),( Eunji Lee ),( Seongjin Jang ),( Suyoung Kim ),( Jeonghwan Han ),( Kwanghee Han ) 한국감성과학회 2015 추계학술대회 Vol.2015 No.-
Animals use camouflage abilities to escape from predators as a survival tactic, likewise, it is critical for people to use effective camouflage strategies in combat situations. Advances in technology have increased demand for adaptive camouflage of military uniforms making it paramount to find the optimal adaptive camouflage pattern for various environments. Previous studies examined effectiveness of color-combinations for camouflage patterns according to various environmental contexts. In this study, researchers aim to identify the optimal camouflage patterns and the exclusion of properties of the colors. Camouflage pattern combination strategies used in this experiment were generated by manipulating the size (small vs. medium vs. large) and contrast (high vs. middle vs. low). We performed the visual search tasks using photo-simulation to evaluate the effectiveness of camouflage strategies. Each stimulus of pattern strategy was presented in three environmental contexts (Woodland vs. Rural vs. Urban) and performance (error rate) was measured. As a result, the pattern composed of middle level of contrast and medium size appeared to be more effective than the others in the Urban context. In the Woodland context, low contrast and small size of patterns were superior to the others. In Rural conditions, strategies with middle contrast and large size were better than the other strategies. This study aims to lay the foundation for optimal adaptive camouflage patterns in different environmental contexts and provide a theoretical basis for future military uniforms.
진동형 히트파이프 단열부 내경 변화에 관한 열 성능 연구
김민선(Minsun Kim),박철우(Chulwoo Park),김상식(Sangsik Kim),김대중(Daejoong Kim) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12
The temperature behavior of Pulsating Heat Pipe (PHP) according to the diameter change were studied by limiting the diameter change to only the adiabatic section. To investigate details of temperature behavior, 4 types of PHP are fabricated. Type 1 and 3 have the same inner diameter of evaporator, condenser and adiabatic section of 2 and 3 mm respectively. The diameters of evaporator, condenser and adiabatic section of type 2, 4 are 2/2/3 and 3/3/2 mm respectively. PHP is set up vertically and activated with bottom heat mode. Ethanol is adopted as working fluid and the filling ratio is fixed at 60%. To investigate operation of PHP in various heat input, heat input power was increased from 10 to 140 W with 10 W step. The time for each heat input step is 1200 seconds. As a result of the experiment, PHP type 3 shows the best performance with the thermal resistance of 0.05℃/W at 140 W heat input.
Yoonmok Park,Minsun Kim,Jeongsug Kim,Baek Hie Nahm 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
With the development of next generation sequencing (NGS) technology, the variation of sequences represented as SNP between cultivars becomes available at genome level. The major domestic cultivars with high yield have been developed by breeding of indica and japonica, it is important to localize the region of origin according to the genotype for further characterization of unique features of cultivars. For the localization of SNP at genome level, the paired end sequences of 6 major domestic rice cultivars, Ilmi, Ilpoom, Sulgaeng, Bakjinju, Hwayoung and Woonkwang were compared against Japonica and Indica Rice Genomes as reference genomes. The genomic DNAs were prepared from callus tissues and paired-end of the fragments were sequenced with NGS Sequencer, Illumina HISeq. About 50x coverage of paired-end sequences were trimmed according to the quality of the sequences, and errors were corrected with statistical analysis of kmers of 15. The trim-corrected sequences were mapped and variants were analyzed against reference genomes. The overall change rate of Ilmi against Nipponbare IRGSP 1.0 and Indica BGI 93-11 reference genomes were 0.92 base/1kb (1/1,079 base) and 8.09 base/1kb (1 base/123 bases), respectively. Among 6 cultivars, overall rate of Bakjinju showed the lowest overall change rate of 0,53 base/1kb, and Hwayoung showed highest frequency of 0.92 base/1kb. Compared to high level in the range of change rate of 7.0-9.3 base/1kb against indica, domestic cultivars showed lower range of change rate 0.2-3.3 base/1kb with unique local high peak against japonica genome depend on the chromosomes. Compared to assembly of genome sequences, the variation of nucleotides compared to reference sequences is much faster and simple to characterize the genotype. The types of variation and the effect on functional categories will be presented.