응집성 효모에 의한 고생산성 알콜 발효

손석민,김인규,변유량 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.5

침강조의 중앙에 깔대기 모양의 판을 설치하고 방해판 아래 다시 공기 방울과 CO_2 가스의 별도 배출구를 설치하여 상부로의 난류를 방지하므로 침강 효율이 크게 향상되도록 settler를 고안하였다. 이 settler를 설치한 탑형 발효조에서 응집성효모 S. uvarum의 에탄올 생산성을 검토한 결과 방해판을 설치하지않은 단순한 원통형 settler에 비하여 1.5배 증대되었다. 또한 탑형 발효조에서 균체 순환율이 에탄올 생산성에 미치는 영향을 살펴본 결과 균체 순환율 1∼5 사이에서는 에탄올 생산성의 변화가 거의 없었으나 6 이상에서는 균체가 유출되므로 에탄올 생산성이 감소하였다. 글루코오스 200g/ℓ의 배지를 공급하면서 균체 재순환 연속 에탄올 발효를 한 결과 침강조의 침강성이 매우 우수하여 희석율 2.Oh^-1까지 조업이 가능하였으며, 희석율 1.1h^-1에서 최고 에탄올 생산성 64.Og/ℓ·h를 얻었다. 이 때 잔당은 80.3g/ℓ, 균체 농도는 88g/ℓ, 생성된 에탄올 농도는 58.8g/ℓ였다. 이와같은 생산성은 현재까지 보고된 응집성 효모의 에탄올 생산성에 비하여 2∼3배 향상된 매우 우수한 결과이다. A tower fermentor equipped with a modified settler was used for ethanol fermentation using highly flocculating yeast, Saccharomyces uvarum. The settler was constructed of glass column divided into two chambers by a funnel shaped divider. Gas was allowed to escape from lower chamber of the settler through a small tube. This design significantly reduced the turbulence in upper chamber of the settler and made it possible to operate at high dilution rate. Using the tower fermentor, the effects of operating conditions such as initial glucose concentration, dilution rate and cell recycle ratio were studied. The maximum ethanol productivity, 64.0 g/ℓ·h was obtained at a dilution rate 1.1 h^-1 and a cell recycle ratio 5 with the corresponding ethanol concentration of 58.8 g/ℓ, and cell mass of 88 g/ℓ.

국산 체외 충격파 쇄석기의 음향학적 특성 평가

최민주,이종수,김성삼,조성찬,양형석,손종수,천원기 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

In the study we measured and evaluated the acoustical property of a domestic spark gap type extracorporeal shock wave lithotripsy (ESWL). Altering the discharging medium between the electrodes of the shock wave generator (water, 1% an 10% NaCl electrolyte), we measured shock waveforms and sound pressure level (SPL). For the shock waves produced using the discharging medium, water, it was seen that the shock wave peak pressure went up to 70 MPa, and the SPL was in the range of 90 - 100 dB. As the concentration of NaCl increased in the electrolyte. the SPL did not change much and was shown to increase correlation with the discharging voltage. In the case of the discharging medium. 10% NaCl electrolyte, it was found that the shock amplitudes varied in a narrow range when being high in the concentration of NaCl and were large when being high in discharging voltages. While the stone fragmentation was in process, we measured the acoustic emission and compared their spectral properties.

좌신 동맥류에 시행한 비동맥-신동맥 우회로술 1예

이석렬,손민수,강재희,박호철 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.1

Renal artery aneurysms are rare, in less than 1% consecutive abdominal aortograms. Renal artery aneurysms are bilateral in about 10% of cases. Controversy persists regarding the indications for repair of renal artery aneurysms and optimal method of repair. The authors report a case of renal artery aneurysm with right renal atrophy in a 37-year-old male patient. This patient has no past medical history including hypertension. On physical examination, a pulsatile mass was palpated in left upper abdomen. Preoperative abdominal CT showed right renal atrophy and 8 cm diameter left renal aneurysm. Abdominal aortogram and left renal angiogram showed huge lobulated outpouching contrast collection at the proximal left renal artery without definite neck and diffuse irregularity at the proximal left renal artery. This patient was treated by splenectomy, thrombectomy, aneurysm resection and end to end splenorenal bypass. No remarkable postoperative complication was observed. The patient was doscjarged postoperative 12 days.

VIP를 분비하며 신경절신경종의 분화를 가지는 갈색 세포종 1예

나민아,김상수,김동욱,최재훈,이태근,공옥녀,안준협,김지연,성한동,정철호,손석만,김인주,김용기 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

갈색 세포종은 신경내분비종양의 하나로 VIP를 포함한 다양한 신경내분비 펩타이드를 분비할 수 있다. 저자들은 만성 설사와 체중 감소를 주소로 내원한 환자에서 좌측 부신에 VIP를 생성하는 갈색 세포종을 진단하여 성공적으로 제거한 후 증상이 모두 호전된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Vasoactive intestinal peptide (VIP) is a 28-amino acid peptide, which may cause secretory diarrhea by stimulating the production of adenylate cyclase. Neuroendocrine tumors, secreting vasoactive intestinal peptide (VIP), are almost always of a pancreatic in origin. However, a pheochromocytoma may produce several neuropeptides, containing VIP, as they are considered to be neuroendocrine tumors. A 57-year-old woman, who presented with chronic watery diarrhea, hypokalemia, weight loss and a left adrenal mass, is described. Histologically, the tumor was diagnosed as a pheoch-romocytoma, with ganglioneuronal differentiation, and was histochemically confirmed to produce a vasoactive intestinal polypeptide. A left adrenal VIP-producing pheochromocytoma was successfully resected. After surgery, her diarrhea subsided and the electrolytes, affected neuroendocrine hormone levels, blood pressure and blood sugar level were normalized (J Kor SOC Endocrinol 18:227-231, 2003).

Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

혼화재 및 폴리프로필렌섬유의 사용에 따른 콘크리트의 품질변화에 대한 기초적 연구

김정섭,손순채,조창호,최진석,김민국 朝鮮大學校 建設技術硏究所 2001 建設技術硏究 Vol.21 No.1

This study is to identify the effect of using admixture and polypropylene fiber on the quality of concrete. The results of the changes in the quality of concrete that has not yet hardened and concrete that has hardened is as follows: 1. Depending on the proportions of admixture, the greater the fly ash content and the lower slag and silica fume, the greater the slump. Admixture and polypropylene together contributed to increased slump. 2. The more fly ash, slag and silica fume added, the less the air. 3. After 360 days of age, greater fly ash content contributed to a lower compressive strength, while greater slag added to higher compressive strength. Silica fume shows a high compressive strength when 10% of silica fume was added on the 360th day of age. 4. Tensile Strength at 360 days of age, the less fly ash and the more slag, the higher the tensile strength. Silica fume at 360 days of age increase strength when the mixing proportion was 30%. 5. The more fly ash and the less slag and silica fume that were mixed, the greater the fire resistance. When added to and polypropylene fiber, the fire resistance of concrete of no effect. 6. The more fly ash and silica fume, the more carbonation shown and when there was only 10% slag present, the neutralization was also shown.

Carbamazepine Degradation by Photolysis and Titanium Dioxide Photocatalysis

Im, Jong-Kwon,Son, Hyun-Seok,Kang, Young-Min,Zoh, Kyung-Duk Wiley (John WileySons) 2012 Water environment research Vol.84 No.7

<P>We investigated the degradation of carbamazepine by photolysis/ultraviolet (UV)-C only and titanium dioxide photocatalysis. The degradation of carbamazepine by UV-only and titanium-dioxide-only (adsorption) reactions were inefficient, however, complete degradation of carbamazepine was observed by titanium dioxide photocatalysis within 30 min. The rate of degradation increased as initial carbamazepine concentration decreased, and the removal kinetics fit well with the Langmuir-Hinshelwood model. The addition of methanol, a radical scavenger, decreased carbamazepine removal, suggesting that the hydroxide radical played an important role during carbamazepine degradation. The addition of oxygen during titanium dioxide photocatalysis accelerated hydroxide radical production, thus improving mineralization activity. The photocatalytic degradation was more efficient at a higher pH, whereas the removal of carbamazepine and acridine (a major intermediate) were more efficient under aerobic conditions. The mineralization of carbamazepine during photocatalysis produced various ionic by-products such as ammonium and nitrate by way of nitrogen dioxide.</P>

사람 위선암에서 cathepsin L의 발현증가

홍원선,홍석일,박인철,손영숙,정훈용,양석균,김해련,민영일 울산대학교 의과대학 1996 울산의대학술지 Vol.5 No.1

cathepsin L은 lysosomal cysteine 단백분해효소로서 기저막(basement membrane)과 세포외기질(extracellular matrix)을 파괴하여 암세포의 침윤과 전이에 중요한 역할을 하는 물질로 알려져 있다. 이러한 cathepsin L에 대한 mRNA 발현도를 5개의 사람 위선암(gastric adenocarcinoma) 세포주와 5명의 위선암 환자에서 채취한 조직에서 방사능으로 표지된 cathepsin L특이 cDNA를 사용한 Northern blot법으로 측정하였다. 위암의 전이병소에서 수립한 세포주인 SNU-5, SNU-16, MKN-45와 Kato Ⅲ에서는 cathepsin L mRNA가 발현되었으나 원발병소에서 수립한 AGS 세포주에서는 mRNA의 발현이 관찰되지 않았다. 5명의 위암 환자에서는 원발병소, 전이가 확인된 임파절 및 암 근처 정상 위점막에서 각각 조직을 채취하여 cathepsin L mRNA의 발현을 측정하였다. 원발병소와 전이병소에서는 모두 cathepsin L mRNA가 발현되었으나 정상 위점막조직에서는 전예에서 mRNA 발현이 관찰되지 않았다. 한편 mRNA의 발현도는 1예에서는 전이병소가 원발병소에 비해 높았으나, 2예에서는 전이병소에서 발현도가 낮았으며, 나머지 2예에서는 원발병소와 전이병소 사이에 차이가 없어, 원발병소와 전이병소 사이에 mRNA의 발현도의 일관성 있는 경향은 관찰되지 않았다. 이상의 결과는 cathepsin L은 위암의 발생과 진행에 있어 암세포의 침윤과 전이를 촉진하는 것 이외에 또 다른 역할을 할 가능성을 시사하고 있다고 사료된다. Cathepsin L, a lysosomal cysteine protease, is known to play an important role in cancer invasion and metastasis by degrading the components of basement membrane and extracellular matrix. The mRNA expression of cathepsin L was determined by Northern blot analysis using a radiolabeled cDNA specific for cathepsin L in five human gastric adenocarcinoma cell lines and five surgical specimens of primary gastric adenocarcinomas, their metastatic lymph nodes and matched adjacent normal mucosae. The mRNA of cathepsin L was expressed in all of the four cell lines established from the metastatic sites, SNU-5, SNU-16, MKN-45 and Kato Ⅲ, while not detected in one cell line established from the primary site, AGS. The mRNA was expressed in all of the five primary and five metastatic cancer specimens tested, while it was not detected in all matched normal mucosae. The intensities of the mRNA expressions, however, did not show the consistent pattern between primary sites and metastatic lymph nodes. These results suggest that cathepsin L may have the other function in addition to facilitation of the invasion and metastasis during the development and progression of stomach cancer.

[P3-20] Development and Characterization of High Quality Salted Mackerel using Enzyme Hydrolysates of Ecklonia cava

Min Seok Yoon,Hyung Jun Kim,Kwon Hyun Park,Jin-Soo Kim,Jeong-Suk Lee,You-Jin Jeon,Hee Jin Son,Min soo Heu 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11

Physicochemical Qualities and Consumer Perception of Tomato Sponge Cakes

Seok Min Son,Jun Ho Lee 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.4

The effects of differing baking ingredient formulations on physicochemical qualities and consumer preferences were investigated using sponge cakes incorporated with tomato powder, a healthy and beneficial food ingredient, as a model system. Tomato powder was incorporated into cake batter at four different amounts (0%, 10%, 20%, and 30%, w/w) by replacing equivalent amount of wheat flour. After appropriate mixing, sponge cakes were baked and cake quality attributes were evaluated after cooling. Specific volume decreased with an increase in the tomato powder substitution, although not significantly (p>0.05). On the other hand, baking loss increased from 10.3 (control) to 13.4 (30% sample) as the tomato powder level increased in the formulation. Lightness (L<SUP>*</SUP>) decreased significantly from 79.5 to 74.1 whereas the firmness significantly increased with the higher incorporation of tomato powder (p<0.05). The consumer preferences on color, taste, and flavor, but not softness, were significantly affected by the amount of tomato powder incorporated in the sample (p<0.05). With respect to overall acceptability, the 20% sample received the highest mean score of 5.1, although this was not significantly different from the 10% sample or control (p>0.05). The incorporation of tomato powder, up to 20%, in the formulation of sponge cakes did not significantly influence the consumers" acceptability in all attributes tested.