외과 영역의 복강경 수술 현황

정호근,오민구 東國大學校醫學硏究所 2004 東國醫學 Vol.11 No.1

복강경 수술이 도입된 이래 기구와 술기의 발달로 복강경 수술의 발전과 적용의 확대를 가져왔다. 소개당시 담낭절제술, 충수절제술 등 기본적인 수술에 국한되었던 복강경 수술이 현재는 다양한 장기의 수술에 이용되고 있다. 이것은 많은 장점을 가지는 최소침습수술(minimally invasive surgery)로서 받아 들여지게 되었다. 최근의 수술 술기의 발달, 의료 기기나 기구들의 발전은 기존의 많은 외과 수술을 복강경 수술로 대체하고 있다. 복강경 수술의 적응이 확대된 현재 외과영역에서 이용되는 여러 종류의 복강경 수술에 대하여 살펴보는 것도 의미가 있을 것이다. After introduction of laparoscopic surgery in the general surgical field, laparoscopic surgery has improved and was widely practiced with development of medical instruments and surgical technologies. Initially laparoscpic surgery had been limited to a basic operation such as cholecystectomy and appendectomy, but now it has been used to a variety of surgery in several organ. As a minimally invasive surgery, laparoscopic surgery has a number of merit. To date, by improving surgical skills and developing medical equipments and instruments, many kinds of the traditional open surgery were replaced by a laparoscopic surgery. With expanding indications of the laparoscopic surgery, this article reviews the current status of the laparoscopic surgery in the surgical field.

다양한 적송잎 추출물의 항생제 내성 황색포도상구균에 대한 항균활성 검증

Nam-Young Kim(김남영),Min-Kyung Jang(장민경),Myung Je Jeon(전명제),Dong-Geun Lee(이동근),HyeJi Jang(장혜지),Seung Woo Lee(이승우),Mihyang Kim(김미향),Sung Gu Kim(김성구),Sang-Hyeon Lee(이상현) 한국생명과학회 2010 생명과학회지 Vol.20 No.4

병원 내 감염의 주된 원인균으로 알려진 황색포도상구균(5. aureus)과 항생제 내성 황색포도상구균(PRSA, MRSA)에 대해 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 적송잎을 대상으로 열수-헥산(HWH), 열수-에탄올(HWE), 헥산, 에탄올 및 열수 등으로 획득된 추출물들로 항균활성을 조사하였다. 헥산, 에탄올, 열수-헥산, 열수-에탄올 추출물들은 그람양성 대표세균인 고초균(B. subfilis), 황색포도상구균 표준균주, 항생제 내성균주인 PRSA 및 MRSA에 대해서 항균활성을 나타내었으며, 특히 MRSA (ATCC 33591) 균주에 대해 다른 균주들 보다 높은 항균활성을 나타냈다. 열수 추출물은 황색포도상구균 표준균주 및 MRSA에서만 항균활성을 나타냈지만, 항균활성은 가장 높아 50 ㎎/㎖의 농도에서 33 ㎜의 생육저해환을 나타냈다. 각 균주들에 대한 생장의 최소저해 농도(MIC)는 HWH 및 HWE 추출물이 모두 0.05 ㎎/㎖의 농도로 가장 낮았고, 열수추출물이 0.5 ㎎/㎖의 농도였으며, 에탄올 추출물은 5 ㎎/㎖의 가장 높은 농도를 나타냈다. 모든 추출물들은 121℃에서 20분간의 열처리 후에도 항균활성이 유지되었으므로 열에 대해 안정한 물질임을 알 수 있었다. 본 연구결과로 식품산업에서 솔잎 추출물을 천연 항균제로 사용할 수 있을 것으로 기대할 수 있다. investigated antimicrobial activities of various pine (Pinus densiflora) needle extracts against antibiotic resistant strains of Staphylococcus aureus. Hot water extract showed the highest antimicrobial activity against normal and methicillin resistant Staphylococcus aureus (MRSA), however, it exhibited no antimicrobial activity against penicillin resistant S. aureus (PRSA). Hot water-hexane (HWH), hot water-ethanol (HWE), hexane, and ethanol extracts showed antimicrobial activity against S. aureus, PRSA and MRSA. Minimum inhibitory concentrations (MIC) of HWH, HWE, hexane, and ethanol extracts were 0.05, 0.05, 0.5 and 5 ㎎/㎖ respectively, and HWH and HWE extracts showed the strongest antimicrobial activity among these extracts. Antimicrobial activities of pine needle extracts were stable after heating at 121℃ for 20 min. These results suggested that pine needle extracts can be used as an effective natural antimicrobial agent for food and medical industries.

전기오븐에서 과열증기주입에 따른 열처리가 닭고기의 이화학적 특성변화에 미치는 영향

천지연,권봉구,이수현,민상기,홍근표,Chun, Ji-Yeon,Kwon, Bong-Gu,Lee, Su-Hyun,Min, Sang-Gi,Hong, Geun-Pyo 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.1

This study was carried out to observe the effect of superheated steam combined with oven heating on the physico-chemical and sensory properties of chicken meat. Specially, chicken breasts and thighs were heated for 40 min in various heating formulations such as oven heating, superheated steam heating or a combination of two kinds of heating. In the physical properties measurement, the shear force was increased as superheated steam heating time and chicken thighs were higher than chicken breasts in all treatments (p<0.05). The highest level of water holding capacity was solely superheated steam treated chicken for 40 min (p<0.05). The $L^*$ value was decreased but $a^*$ value or $b^*$ value were increased after cooking. Chicken breast exhibited a higher colour value than chicken thigh. Superheated heating was effective to reduce heating loss as 22.64% (p<0.05). However, pH was not different depending on the heating formulation or part of the chicken meat (p>0.05). In the sensory test, the combination of 10 min oven heating and 30 min superheated steam heating was effective to create a good flavour of chicken meat. In this study, an optimum formulation was developed which was a combination of 10 min oven heating and 30 min superheated steam heating. It was more effective to improve the quality of chicken meat than the single heat treatment of chicken meat.

S-126 : Screening and Preemptive Therapy for Reactivation of Hepatitis B Virus Associated with Infliximab

( Min Geun Gu ),( Heon Ju Lee ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Background and aims: Reactivation of hepatitis B virus (HBV) associated with infliximab has become an issue, because of widespread use of infliximab. Infliximab, anti-tumor necrosis factor (TNF) α inhibitor, is used for treatment of immune-mediated diseases such as rheumatologic and dermatologic diseases as well as inflammatory bowel diseases. TNF-α inhibitors may result in the frequent occurrence of increased susceptibility or reactivation of concurrent infection. This study aimed to investigate performance of screening and preemptive therapy for reactivation of HBV induced by infliximab in real-world. Methods: Between December 2004 and January 2013, a total of 51 patients received infliximab therapy in Yeungnam University Hospital. We retrospectively reviewed all medical records and analyzed performance of serologic screening and preemptive therapy of HBV, and evaluated patients with reactivation of HBV. Results: Median age of enrolled patients was 33.0 years. Male was predominant (66.7%). The most common cause of use of infliximab was Crohn`s disease (68.6%). 39 patients (76.5%) were evaluated for HBsAg, and 32 patients (62.7%) were checked for IgG anti-HBc. No serologic test for HBV was conducted in 12 patients (23.5%). HBsAg positive was detected in 2 patients (3.9%), and IgG anti-HBc positive was detected in 11 patients (21.6%), respectively. In total, 11 patients underwent current or post HBV infection. Reactivation of HBV developed in two patients (2/11, 18.2%) with HBsAg seropositivity. The two patients received treatment with entecavir, and achieved complete and partial virological response, and biochemical responses. Conclusions: Our study showed that adherence of screening and preemptive therapy for HBV prior to infliximab therapy was neglected. More attention for reactivation of HBV should be paid to patients before infliximab therapy.

밴드결찰술을 이용한 직장 신경내분비종양의 내시경 치료 결과

구민근 ( Min Geun Gu ),이시형 ( Si Hyung Lee ) 대한소화기학회 2018 대한소화기학회지 Vol.72 No.3

목적: 직장 신경내분비종양의 치료에서 밴드결찰술을 이용한 점막하절제술은 점막절제술에 비하여 시술시간이 짧고 합병증의 발생이 적은 것으로 보고된다. 본 연구에서는 밴드결찰술을 이용한 점막하절제술이 기존의 점막절제술보다 더 우수한지 알아보고자 하였다. 대상 및 방법: 2007년 3월부터 2017년 5월까지 직장 신경내분비종양으로 밴드결찰술을 이용한 점막하절제술과 점막절제술로 치료한 환자를 대상으로 분석하였다. 결과: 총 148명의 환자가 등록되었으며, 120명은 밴드결찰술을 이용한 점막하절제술로 치료하였고, 28명은 점막절제술로 치료하였다. 밴드결찰술을 이용한 점막하절제술이 완전 절제율과 치유 절제율에서 점막절제술보다 우수한 성적을 보고하였다(93.3% vs. 75.0% and 92.5% vs. 71.4%, p=0.009 and p=0.003, respectively). 결론: 직장 신경내분비종양의 치료에서 밴드결찰술을 이용한 점막하절제술은 점막절제술보다 더 우수한 치료 술기이다. Background/Aims: Ligation-assisted endoscopic submucosal resection (ESMR-L) is preferred for the treatment of rectal neuroendocrine tumors because its results are better than those for endoscopic mucosal resection (EMR), and the procedure time is shorter and the incidence of complications is lower than endoscopic submucosal dissection. The aim of this study was to evaluate the clinical usefulness of ESMR-L compared with EMR for rectal neuroendocrine tumors. Methods: From March 2007 to May 2017, 148 patients diagnosed with rectal neuroendocrine tumors were divided into ESMR-L and EMR groups and analyzed retrospectively. Results: Of 148 patients with rectal neuroendocrine tumor, 120 had ESMR-L and 28 had EMR. The ESMR-L group had a significantly higher rate of complete resection and curative resection than the EMR group (93.3% vs. 75.0% and 92.5% vs. 71.4%, p=0.009 and p=0.003, respectively). Conclusions: ESMR-L for the treatment of small rectal neuroendocrine is a significantly superior modality to EMR. (Korean J Gastroenterol 2018;72:128-134)

키틴분해 곰팡이 Penicillium janthinellum LPB-01의 생물학적 특성 연구

구본근 ( Bon Geun Koo ),백구 ( Gu Baek ),신서윤 ( Seo Yoon Shin ),김민정 ( Min Jung Kim ),김태준 ( Tae Jun Kim ),선준홍 ( Jun Hong Seon ),전소영 ( So Young Jeon ),임다영 ( Da Young Im ),박지민 ( Ji Min Park ),박제권 ( Jae Kweon P 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.4

The aim of the present study is to define the characteristics and biological activities of chitinolytic fungus Penicillium janthinellum LPB-01. Chitinolytic activity was determined using 4-nitrophenyl N-acetyl-β-D-glucosaminide (about 417 pmol/ μg/min) and 4-Nitrophenyl N,N`-diacetyl-β-D-chitobiosides (665 pmol/μg/min) as substrates, and reducing sugar assay. P. janthinellum LPB-01 actively degrades chitin and sulfated chitin producing chitooligosacharides. These results revealed that P. janthinellum LPB-01 is suitable for depolymerizing chitinous materials including sulfated chitin to produce low molecular weight chitooligosacharides. In addition, significant anti-oxidant effects of culture supernatant of P. janthinellum LPB-01 cultured in different conditions were determined by using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical. Based on these results, we concluded that P. janthinellum LPB-01 can be used as a produced for potentially active materials.

Diameter Effect of Silver Nanorod Arrays to Surface-enhanced Raman Scattering

Geun Hoi Gu,Min Young Kim,Hyeok Jin Yoon,서정쌍 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.3

The effect the diameter of silver nanorod arrays whose distance between the nanorods was uniform at 65 nm have on Surface-enhanced Raman Scattering (SERS) has been studied by varying the diameter from 28 to 51 nm. Nanorod length was fixed at approximately 62 nm, which is the optimum length for SERS by excitation with a 632.8 nm laser line. The transverse and longitudinal modes of the surface plasmon of these silver nanorods were near 400 and 630 nm, respectively. The extinction of the longitudinal mode increased with increasing nanorod diameter, while the transverse mode did not change significantly. High-quality SERS spectra of p-aminothiophenol and benzenethiol adsorbed on the tips of the silver nanorods were observed by excitation with a 632.8 nm laser line. The SERS enhancement increased with increasing nanorod diameter. We concluded that the SERS enhancement increases when the diameter of silver nanorods is increased mainly by increasing the excitation efficiency of the longitudinal mode. The enhancement factor for the silver nanorods with a 51 nm diameter was approximately 2 × 107.

Medicinal Chemistry : RESEARCH ARTICLE ; NRF2 Signaling Negatively Regulates phorbol-12-Myristate-13-Acetate(PMA)-Induced Differentiation of Human Monocytic U937 Cells into pro-Inflammatory Macrophages

( Min Gu Song ),( In Geun Ryoo ),( Hye Young Choi ),( Bo Hyun Choi ),( Sang Tae Kim ),( Tae Hwe Heo ),( Joo Young Lee ),( Pil Hoon Park ),( Mi Kyoung Kwak ) 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

Blood monocytes are recruited to injured tissue sites and differentiate into macrophages, which protect against pathogens and repair damaged tissues. Reactive oxygen species (ROS) are known to be an important contributor to monocytes` differentiation and macro-phages` function. NF-E2-related factor 2(NRF2), a transcription factor regulating cellular redox homeostasis, is known to be a critical modulator of inflammatory responses. We herein investigated the role of NRF2 in macrophage differentiation using the human mono-cytic U937 cell line and phorbol-12-myristate-13-acetate(PMA). In U937 cells with NRF2 silencing, PMA-stimulated cell adherence was significantly facilitated when compared to control U937 cells. Both transcript and protein leves for pro-inflammatory cytokines, includ-ing interleukine-1β(1L-1β), 1L-6, and tumor necrosis factor-a (TNFa) were highly elevated in PMA-stimulated NRF2-silenced U937 compared to the control, In addition, PMA-induc-ible secretion of monocyte chemotactic protein 1 (MCP-1)was significantly hign in NRF2-silenced U937. As an underlying mechanism, we showed that NRF2-knockdown U937 retained high levels of cellular ROS and endoplasmic reticulum (ER) stress markes expres-slion;and subsequently, PMA-stimulated leves of Ca2+and PKCa were greater in NRF2-knockdown U397 cells, which caused enhanced nuclear accumulation of nuclear factor-kB (NFkB) p50 and extracellular signal-regulated kinase (ERK)-1/2 phosphorylation. Whereas the treatment of NRF2-slienced U937 cells with pharmacological inhibitors of NFkB or ERK1/2 largely blocked PMA-induced 1L-1βand 1L-6 expression, indicating that these pathways are associated with cell differentiation. Taken together, our results suggest that the NRF2 system functions to suppress PMA-stimulated U937 cell differentiationinto.

Anti-Obesity Effect of Polysaccharies isolated from Macroalgae Tott

Gu Baek,Bon Geun Koo,Yeon Woo Kim,Min Joo Kim,Jae Kweon Park 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Clinical outcomes of medically treated acute aortic intramural hematoma (초)

( Min Kyu Kang ),( Jong Seon Park ),( Won Jong Park ),( Ung Kim ),( Sang Hee Lee ),( Dong Gu Shin ),( Young Jo Kim ),( Yoon Kyung Cho ),( Chang Wook Nam ),( Seung Ho Hur ),( Jin Bae Lee ),( Jae Geun R 대한내과학회 2012 대한내과학회 추계학술대회 Vol.2012 No.1