Developmental Competence of Bovine Embryos Cultured In Vitro According to Culture Methods

Min CS,Son GD,Chung WJ,Song SH,Rho CW,Kang YS,Kong IK,Park CS 한국동물번식학회 2007 Reproductive & Developmental Biology(Supplement) Vol.31 No.2s

Developmental Competence of Bovine Embryos Cultured In Vitro According to Culture Methods

Min CS,Son GD,Chung WJ,Song SH,Rho CW,Kang YS,Kong IK,Park CS 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.2

난소에 발생한 양성 섬유성 조직구종 1 예

진건,정을영,민창수 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.11

Benign fibrous histiocytoma is a tumor composed of a mixture of fibroblastic and histiocytic cells that are often arranged in a cartwheel or storiform pattern and accompanied by varying number of inflammatory cells, foam cells, and siderophages. Benign fibrous histiocytoma represents one of the common soft tissue tumors with most cases presenting in the skin. The common sites of incidence are the limbs, trunk, head, neck, and posterior peritoneal cavity:less commonly, these lesions arise in female reproductive organs. This report documents a case of benign fibrous histiocytoma arising in the ovary of 48 year-old female whose chief complaint was abdominal pain.

Effect of Size of Corpus Luteum on Pregnancy Rate Following Transfer of Hanwoo Embryos Produced In Vivo

Son GD,Chung WJ,Song SH,Min HS,Kong IK,Park CS 한국동물번식학회 2007 Reproductive & Developmental Biology(Supplement) Vol.31 No.2s

Effect of Size of Corpus Luteum on Pregnancy Rate Following Transfer of Hanwoo Embryos Produced In Vivo

Son GD,Chung WJ,Song SH,Min HS,Kong IK,Park CS 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.2

양수내 Creatinine치에 관한 연구

김영석(YS Kim),민병철(BC Min),이명아(MA Lee),박창서(CS Park),박찬용(CY Park),조성진(SJ Cho) 대한산부인과학회 1988 Obstetrics & Gynecology Science Vol.31 No.10

정확한 태아 성숙도 판정을 위해서 임신 제 35주에서 제 41주까지의 정상임부 35례, 자간전 증을 합병한 임부 28예에서 양수와 모체혈청의 creatinine concentration측정 및 shake test를 동시에 실시하였다. 그리고 전례에서 호흡장해증후군의 발생을 관찰하여 위의 검사결과에 입각한 태아 성숙도 판정을 시도하여 다음과 같은 결론은 얻었다. 1. 양수내 creatinine concentration 및 AF/MS비는 정상임신군 및 자간전증군에서 임신주수에 따라 점차로 증가하였다. 2. 자간전증군의 양수와 모체혈청 creatinine concentration 및 평균 AF/MS비는 정상임신군의 수치보다 유의하게 높았다. 3. 태아 성숙기준을 임신 제 37주이상으로 정하였고 양수 creatinine concentration 2.0mg%이 상, AF/MS비 2.5이상 그리고 positive shake test를 성숙기준으로 선택할 때 ,그 정확도는 정 상임신군에서는 85.7%, 82.9% 88.9%이었고, 자간전증군에서는 89.3% 95.7% 92.9% 이었다. 그리고태아체중 2500g을 성숙기준으로 하고 똑같은 방법으로 예측한 태아 성숙판정의 정확 도는 정상임신군에서는 88.6% 91.4% 85.7% 이엇고, 자간전증군에서는 75.0% 78.6% 75.0%ㅇ 었다. 4. 총 7예의 신생아에서 신생아 호흡장해증후군이 발생했는데 4례는 정상임신군에서 3례는 자간전증군에서였다. Accurate estimation of fetal maturity is neccessary where termination of pregnancy before term is indicated. To predict the fetal maturity creatine concentration of amniotic fluid and maternal serum were measured and shake test was performed simultaneously in the 35 normal pregnancy women and 28 preeclamptic women between 35 to 41 gestational weeks. The results were as follows; 1. The creatinine concentration of amniotic fluid and aminiotic fluid/maternal serum concentration of creatinine (AF/MS) ratio increased gradually related to gestational weeks in normal and preeclamptic pregnancy. 2. The mean amniotic fluid and maternal serum creatinine concentration and the mean AF/MS ratio in preeclamptic pregnancy were significantly higher than those inf the the normal pregnancy. 3. Assuming a creatinine concentration 2.0mg% or greater in the amniotic fluid, AF/MS ratio of 2.5 or greater and positive shake test to corespond with 37 weeks of gestational age, the accuracy in predicting fetal maturity was 85.7%, 82.9% and 88.9% in normal pregnancy and 89.3% , 95.7% and 92.9% in preeclamptic pregnancy, respectively. Assuming same indication to correspond with 2500 mg of fetal weight , the accuracy in predicting fetal maturity was 88.6%, 91.4% and 85.7% in normal pregnancy and 75%, 78.6% and 75% in preeclamptic pregnancy, respectively. 4. Total 7 neonates developed respiratory distress syndrome, four in normal group and remainder in preeclamptic pregnancy group.

폐경기여성에서 에스트로젠 보충요법시 병행투여된 프로게스테론제제가 골밀도에 미치는 영향

김동진,진건,신용덕,김수련,박교만,정을영,민창수 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.2

Estrogen replacement therapy is known to reduce the incidence of cardiovascular disease and osteoporosis in postmenopausal women. Estrogen has beneficial effect on bone density and adding progesterone to estrogen is known to have synergistic effect. However, the effect of progesterone on bone mineral density had not been precisely evaluated in Korean postmenopausal women. To evaluate the impacts of progesterone on bone mineral density during estrogen replacement therapy, we conducted the randomized 2-year trials of conjugated equine estrogen (Premarin 0.625 mg/day) with or without cyclic progesterone(medroxyprogesterone acetate : MPA 10 mg/day for 12 days) in 105 postmenopausal women. Bone mineral density was measured in the lumbar vertebrae(L2-L4) using dual energy X-ray absorptiometry(DEXA) at the beginning of the treatment, 6 onths, 12 months and 24 months later, respectively. In Premarin only group, bone mineral density at 6 months and 12 months after treatment did not show significant difference from baseline value. But bone mineral density on 24 months after treatment showed significantly increased value(p value$lt;0.05) than that of initial. In Premarin plus MPA group, bone mineral density at 12 months and 24 months after treatment showed significantly increased than baseline(p value$lt;0.05). And compared with Premarin only group, Premarin plus MPA group showed significant increase in spinal bone density after 2 years treatment(p value$lt;0.05). These results suggest that the addition of MPA in estrogen replacement therapy appears to increase in bone mineral density more than unopposed estrogen. Thus combination of estrogen and progesterone appeared to be effective long-term prophylactic treatment regimen upon postmenopausal bone loss.

난소에 재발한 급성백혈병 1 예

김동진,진건,김수련,박교만,정을영,민창수 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.10

Currently attention is focused on the testicle as a site of leukemic relapse. However, leukemic involvement of the ovary has been described in several autopy series with an incidnce of 11 to 50 percents in patients with bone marrow relapse, but has rarely been reported during the clinical course of leukemia. But leukemic involvement of the ovaries has received little attention, and the significance of ovary associated relapse in relation to the eventual outcome of the patient has not been investigated. We experienced one case of acute lymphocytic leukemia(ALL) presenting as ovarian tumor. Involvement was also presented in abdominal and pelvic lymph node, mesentery, omentum, and serosal surface. Here we present the case with brief review of literature.

Interleukin-1이 인간의 과립막황체세포에서 인슐린 유사 성장인자들과 그 결합단백질들의 생성에 미치는 영향

최영민,이진용,문신용,김정구,김석현,민응기,서창석 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.7

인간 난소의 과립막황체세포에서 IL-1의 일부 작용이 IGF계에 영향을 미침으로써 중개될 수 있는가와 이런 작용이 IL-1 수용체를 통해서 이루어지는가를 규명하고자 과립막황체세포를 IL-1α(0.2∼10 ng/ ml), IL-1β(0.2∼10 ng/ml)를 단독 또는 hCG(0.1 g/ ml), IL-1ra(100 ng/ml)와 함께 복합적으로 첨가된 무혈청 배양액에서 배양 후 이중항체 방사면역법, Western ligand blot과 면역침전법으로 측정될 수 있는 IGF들 농도와 IGFBP들의 강도를 비교분석하여 다음과 같은 결과를 얻었다. 1. IL-1α와, IL-1β 모두 0.2∼10 ng/ml 농도에서 IGF-II 생산을 유의하게 증가시켰으나 IGF-I은 과립막황 체세포의 조건 배양액에서 검출되지 않았다. 2. IL-1 0.2 ng/ml에 노출되었던 세포군에서는 배양액 내 IGFBP-1, IGFBP-3, IGFBP-4 축적이 유의하게 감소하였다. 3. hCG는 과립막황체세포의 배양액내 IGFBP들의 축 적을 감소시켰으나, IL-1β 1 ng/ml과 함께 복합적 처치 된 세포군에서 IGFBP-1, IGFBP-4의 축적이 증가되었다. 4. IL-1ra는 단독 첨가시 과립막황체세포의 배양액내 IGFBP들 축적에 영향이 없었으나, IL-1β 0.2 ng/ ml과 함께 복합적으로 첨가한 경우 IL-1β의 IGF-II 생산과 IGFBP들 축적에 대한 작용을 차단하였다. 이상의 결과로 보아 IL-1은 IGF계를 통하여 인간 과 립막황체세포의 기능에 영향을 미칠 수 있으며 IL-1의 이러한 작용은 그 수용체를 통하여 이루어진다고 사료된다. The relationship between the immune system and the endocrine system in the ovary has become of an challenging and exciting subject to reproductive scientists. The intraovarian regulators such as insulin-like growth factor (IGF) system and interleukin-1 (IL-1) are believed to regulate ovarian function. This study was undertaken to investigate the effects of IL-1 on the production of IGFs and IGF binding proteins (IGFBPs) in human granulosa-luteal cells and to evaluate if this action is receptor mediated. The cells were recovered from follicular fluid obtained from women undergoing oocyte retrieval for in-vitro fertilization procedures. Purified granulosa-luteal cell preparations were cultured for 3 days in serum free media supplemented with IL-1, IL-1 receptor antagonist (IL-1ra), human chorionic gonadotropin (hCG) alone or these agents in combination. IGFs and IGFBPs in conditioned media were analyzed using double antibody radioimmunoassay, Western ligand blot and immunoprecipitation. No immunoreactive IGF-I was detectable in all conditioned media of granulosa luteal cells at a detectable level of 2 nM/L. At a concentration of 0.2-10 ng/ml both IL-1 and IL-1β was found to stimulate IGF-II production. The accumulation of IGFBP-1, IGFBP-3 and IGFBP-4 in cultures of human granulosa-luteal cells was inhibited by both forms of IL-1 (0.2 ng/ml). Treatment of granulosa-luteal cell culture with hCG (0.1 g/ml) also resulted in a significant decrease in IGFBPs accumulation but concurrent treatment with IL-1β (1 ng/ml) stimulated the accumulation of IGFBP-1 and IGFBP-4. IL-1ra (100 ng/ml) had no effect on the IGF production and IGFBPs accumulation when applied by itself but administration of an IL-1ra with IL-1β (0.2 ng/ml) neutralized the IL-1β-induced effect on the IGF production and IGFBPs accumulation in granulosa- luteal cells. These observations suggest that IL-1 modulate IGFBPs in human granulosa-luteal cell cultures and that this action of IL-1 is receptor-mediated.