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SH Song,JG Kim,HJ Song,B Mohana Kumar,SR Cho,CY Choe,SH Choi,노규진,SY Choe 사단법인 한국동물생명공학회 2005 Reproductive & developmental biology Vol.29 No.2
The objective of this study was to examine the effect of EGF on meiotic maturation and pronuclear (PN)formation of porcine oocytes. Prepubertal gilt cumulus-oocyte-complexes (COCs) aspirated from 2 6 mm folliclesof abbatoir ovaries were matured in TCM199 containing 0.1 mg/ml cysteine, 0.5 ug/ml FSH and LH, and EGF (0,5, 10, 20, 40 ng/ml) for 22 hr at 39°C in a humidified atmosphere of 5% CO2 in air. They were then cultured foran additional 22 hr without hormones. In Experiment 1, to examine the nuclear maturation at 44 hr of culture, theexpanded cumulus cells were removed by vortexing for 1 min in 3 mg/ml hyaluronidase. The oocytes were fixedin acetic acid : methanol (1:3, v/v) at least for 48 hr and stained with 1% orcein solution for 5 min. Nuclear statuswas classified as germinal vesicle (GV), germinal vesicle breakdown (GVBD), prophase-metaphase I (PI-MI), andPII-MII under microscope. In Experiment 2, to investigate PN formation, oocytes were fertilized with Percoll-treatedfreshly ejaculated sperm (1 × 105 cells/ml) in mTBM with 0.3% BSA and 2 mM caffeine for 5 hr, and cultured inNCSU-23 medium with 0.4% BSA. At 6 hr of culture, the embryos were fixed in 3.7% formaldehyde for 48 hr andstained with 10 ug/ml propidium iodide for 30 min. PN status was classified as no or one PN (unfertilized), 2 PN(normal fertilized) and 3 PN (polyspermy). Differences between groups were analyzed ≥ using one-way ANOVAafter arc-sine transformation of the proportional data. The rate of oocytes that had reached to PII-MII weresignificantly (P<0.05) higher in all groups added EGF than that of non-treated group (67%), but it did not differamong the all added groups (86%, 85%, 79% and 81%, in 5, 10, 20 and 40 ng/ml EGF, respectively). No differenceson the incidence of 2PN were observed in all treated groups (25%, 30%, 33%, 29% and 29%, in 0, 5, 10, 20 and 40ng/ml EGF, respectively), however, in non-treated group, polyspermy tended to be increased (66% vs. 58%, 54%, 52%and 55%, 0 vs. 5, 10, 20, 40 ng/ml EGF, respectively). These results suggest that EGF can be effectively used as anadditive for enhancing oocyte maturation and reducing the incidence of polyspermy in pig. (Key words: Pig oocytes, In vitro maturation, Pronuclear formation, EGF)
진통시 태아심박형 ( Fetal Heart Rate Patterns ) 및 태아혈액 pH 및 태아예후와의 관계
송선희(SH Song),최의숙(ES Choi),정복숙(BS Chung),김종일(JI Kim),우복희(BH Woo),강신명(SM Kang) 대한산부인과학회 1981 Obstetrics & Gynecology Science Vol.24 No.12
1980년 6월부터 8월까지 이화대학병원 산부인과에 입원한 고위험도군의 만삭임산부 56명에 대하여 지속적인 직접태아감사 방법과 태아혈액 pH를 측정하여 태아예후와의 관계를 연구 하여 다음과 같은 결론을 얻었다. 1. 중등도의 변이하강, 만기하강, 연장하강의 평균 태아두피혈 pH는 각각 7.278, 7.259, 7.238 로써 비교적 낮은 경향을 보여주었으며 이들은 역시 Apgar 점수가 6.5, 5.6, 4.7로써 낮은 관 계가 있었다. 2. 무주기변화 및 조기하강을 나타낸 12예는 모두 정상범위의 pH를 나타내었고 변이하강을 나타낸 23예중 acidosis 및 precidosis의 빈도가 각각 4%이었고 만기하강을 나타낸 3예중 1 예가 acidosis를 1예가 preacidosis를 나타내었다. 3. 변이, 만기 및 연장하강이 나타낼때는 과반수이상, 즉 55-100%의 태아장애를 예측할 수 있었다. 4. 직접태아감시 및 태아혈맥 pH측정에 의한 모체 및 태아의 합병증은 없었다. 따라서 직접 태아감시에 있어서 지속적이고 점진적인 변이 또는 만기하강이 나타날때는 태아두피혈 pH 의 반복측정이 태아장애 조기발견에 지침이 될 것으로 추정된다. 1. The mean value of fetal blood pH were relatively low in moderate variable (7.279), late (7.259) and prolonged decerations (7.238) Also, the mean Apgar scores in one minute were sighnificantly lower in moderate variable (6.5) late (5.6) and prolonged deceleration (4.6) than no periodic changes (7.1) 2. All of fetal scalp pH values of no periodic changes (5cases) and early deceleration (7 cases) were above 7.25 pH units. But 9% of variable decerations and 67% of late decerations were below 7.25 pH units. 3. Apgar scores below 6 in one minute were associated with 55% of variable decerations 80% of late decerations and 100% of prolonged decerations 4. There were no specific maternal and fetal complications with applications of scalp electrods and sampling of scalp blood for pH determination.
송승규,김수평,김은중,장은덕,김사진,이귀세라,신재인,이상형 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.5
저자들은 53세된 환자에서 난소에 발생된 원발성 평활근육종 1예를 경험하였기에 간단한 문헌고찰과 함께 보고하는 바이다. Primary leiomysarcomas of the ovary are extremely rare. Fewer than 20 cases have been described in the literature. This cse is occurred in a 53 years old multiparous woman. We experienced a case of pure ovarian leiomysarcoma which may originate from smmoth muscle cells of the ovarian blood vessel. We report this case with a brief view of the literature.