Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

Zinc protoporphyrin과 다양한 용매조건이 MTT formazan의 발색 안정성에 미치는 영향

박경아,최유미,최현아,김미리,김현정,홍정일 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-

An assay method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide (MTT) is commonly utilized for analyzing cell viability. In this method, MTT tetrazolium is converted to formazan by mitochondrial reductase of viable cells. The formzan formed is, however, not stable; the stability is affected by various factors. In the present study, effects of zinc protoporphyrin (ZnPP) and different solvents on color response of formazan dye were investigated. MTT formazan was stable under dark; light enhanced the color degradation of formazan. ZnPP induced rapid degradation of formazan under light condition in DMSO solution. Decolorization of formazan was faster in aqueous solvents, including distilled water, HCl as well as Triton X-100 solution. Formazan dye was most unstable in HCl (0.1 N) regardless of light or presence of ZnPP. Color response of formazan dye in NaOH (0.1 N) was enhanced by ~2 folds as compared to one in DMSO. MTT formazan was stable in this condition; the color change was less than 10% even under light during 2 hr. ZnPP, however, induced rapid decolorizaion of formazan dissolved in NaOH under light. H2O2, an oxidizing agent, did not affect color response of MTT formazan. The present results suggest that various factors could affect the color response of MTT formazan, which needs to be considered carefully in MTT assay. Key words:MTT tetrazolium, formazan, zinc protoporphyrin, cell viability, sodium hydroxide.

청주시 소비자의 그린마케팅에 대한 인식과 그린제품 구매의도

권소영, 박혜영, 최혜미, 최현미, 유현정 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to analyze consumers' perception of green marketing and purchase intention of green products. Questionnaire survey was conducted from 9 August to 29 August 2010. We have surveyed 298 consumers who were living in Cheongju area. Major findings are as follows. First, consumers' environment perception and involvement, awareness of green marketing, evaluation for green products, purchase intention of green products were generally positive. It means that Korean consumers have a positive attitude and behavioral intention for green consumption. Second, consumers responded high perception level for necessity of green marketing and purchase intention of green products. On the other hand, consumers think that green product manufacturers need to more protect our environment. Consumers evaluated the quality of green products is good but its price is high. Third, insufficient information was the most common response of the items asked for the resistance of the whole process for the production of green products. For benefit part, high quality and symbolic value of green consumer were given answer highly. Fourth, there were differences among the consumer groups from environment perception, awareness of green marketing, evaluation for green products, and purchase intention of green products by consumers' characteristics. Old aged group were high involved on environment and student group showed relatively low environment perception and involvement of green consumption. Four million won or less income groups were relatively higher interest in green products and eco-conscious consumer groups have a high degree of purchasing intention of green products.

국가 음악과 교육과정에 기초한 초·중등학교 음악예술프로그램 적용

석문주,권덕원,최은식,최미영,김명숙,박장미,손미현,정지혜 이화여자대학교 사범대학 교육과학연구소 2011 교육과학연구 Vol.42 No.2

In order to increase opportunities for students to enjoy the musical arts, it is necessary to develop musical arts programs that are based on students’ interests and the national (or provincial) curriculum, that can be systematically and effectively supported by teachers. In this regard, the purpose of this study was to develop those musical arts, carry out the programs in one elementary school and one middle school from October to December 2011, and analyze each implementation. For the analysis, lesson videos, students’ worksheets, in-depth interviews with students, teachers, and teaching artists, and lesson plans were collected. The conclusions and implications are as follows: 1) the role and meaning of musical arts programs based on national curriculum were more highlighted; 2) students could have great depth of aesthetic experience by the means of direct contact with professional musicians and concrete musical experience; 3) the role of music mediators and its importance was magnified; 4) there was a positive change in the musical preferences and attitudes of students with regard to the musical artsprograms. 본 연구는 학생들의 음악 예술 향수기회 확대를 위해 학생들의 흥미와 국가 교육과 정을 중심으로 하는 ‘현장성’, 교사들의 음악 교육을 효과적으로 지원할 수 있는 ‘체계성’, 그리고 음악 예술 전문가들과 학교 사이에 꾸준히 실행될 수 있는 ‘지속성’을 갖춘 음악예술프로그램을 2010년 10월부터 12월까지 초등학교와 중학교에 적용하고 그 결과를 분석하고자 하는데 목적이 있다. 본 연구는 수업 동영상, 학생 활동지, 학생과 교사 심층인터뷰, 강사 인터뷰, 수업안 자료를 수집하여 분석하였다. 본 연구의 결론은 첫째, 국가 교육과정을 반영한 음악 예술프로그램의 역할과 의미가 더욱 확장되었다. 둘째, 음악가들과의 직접적인 만남과 구체적인 예술적 체험을 통하여 학생들은 이전과 비교될 수 없는 ‘깊이 있는 미적 경험’을 하게 되었다. 셋째, 음악메디에이터의 역할이 부각되었다. 넷째, 음악예술프로그램을 통하여 학생들에게 음악에 대한 삶의 변화가 있었다.

요통 및 방사통 환자에서 고장성 식염수를포함한 경막외 주사 효과

김현배,김미정,최기섭,이상건 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.1

금연구연 지정에 대한 공공기관 근무자의 태도

최연희,전은영,김현미 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

The purpose of this study is to investigate the attitude of public works on the nonsmoking areas. The subjects were a volunteer sample of 436 public workers in Daegu city. Data was collected through interviews and questionaires from March 10 to May 9, 2001, and analyzed by frequency, percentage, chi-square test and multiple logistic regression, using a SPSS program. The results of this study were as follows: Smokers of the subject group accounted for 47.9%, and smokers who smoke one pack of cigarettes per day and a cigarette to its two-thirds length was the most. 42.6% of smokers have positive attitude to quit smoking, but 36.6% has no intention to quit. Among the subjects who answer 'yes' to designate the nonsmoking area were 63.3%. In the designated places of nonsmoking area within public workplace, 'elevator' showed the highest(95.0%), 'cafeteria'(73.0%), 'physical-training room'(71.6%), and 'passage'(66.4%). For designation of nonsmoking areas, nonsmokers were more positive than smokers and the difference was statistically significant. To designated the factors influencing attitude of public workers on nonsmoking areas, logistic regression analysis has been done. According to the results of this analysis, the volume of smoking and a job barrier being due to cigarette smoking were selected as significant factors. In conclusion, more smoking and health education for public workers is needed to reduce the rate of national smoking and allow people to have a good smoking attitude. It is required to arrange an institutional system who can strictly public workers' smoking in their offices.

학교 건강증진사업을 위한 보건교육전략

최연희,김현미 慶山大學校 保健福祉硏究所 2000 保健福祉硏究 Vol.4 No.-

호주의 정신보건전략에 관한 연구

최현미 한국 정신보건 사회사업학회 2001 정신보건과 사회사업 Vol.12 No.-

본 연구에서는 호주의 정신보건전략을 소개하고 우리의 지역정신보건에 함의하는 바를 생각해 보았다. 호주는 1992년 연방정부가 수립한 정신보건전략을 시작으로 탈시설화를 전폭적으로 시행에 옮김으로써 지역정신보건사업을 확장하여 실시하고 있다. 본 연구에서는 호주의 정신보건제도를 연구하기 위하여 2001년 7월과 8월에 호주 멜버른대학교의 정신보건연구소에 체재하면서 관련기관을 방문하여 시설을 관찰하고 정부 담당자와 정신보건서비스기관의 전문가를 대상으로 면담조사를 실시하였다. 국가정신보건전략 이후 빅토리이주는 주정부의 리더십으로 재정문제해결과 정신보건사업 활성화를 이룩한 것을 살펴보았고 다양한 지역사회서비스의 특성을 살펴보았다. 호주의 지역정신보건의 전문화와 그 진행과정을 통해 우리가 주목할 점으로는, 우선 호주정부가 지역 정신보건에 대한 의지와 전략을 성실히 이행함으로써 재정적인 문제해결은 물론 정신장애인을 위한 지원을 확대한 점과 일정 인구비율에 따라 정신보건서비스가 지역적으로 균형이 잡혀 있고 비교적 접근가능성이 높은 점이다. 호주의 경험을 통해 우리의 정신보건 발전방안을 제안하였다. 중심단어 : 정신보건전략, 탈시설화, 지역정신보건, 팀접근 This study aims to introduce the national mental health strategy in Australia and to discuss about what it means to our mental health system. Starting 1992 Australia planned a national mental health strategy and fully implemented deinstitutionalization of the mentally ill. In order to understand the mental health system I visited community mental health services and had interviews with their mental health workers in the state of Victoria of Australia during my stay at the Mental Health Unit at the School of Social Work, University of Melbourne in the period of July and August, 2001. In the state of Victoria, the state government closed down all of the mental hospitals and incorporated into the general hospital and established the area-based mental health services except the acute inpatient units which have only 20 beds in the general hospital. Looking at the characteristics and problems of Australian community mental health, what we koreans can learn include the Government's will for the improvement of mental health services and the balance and the accessibility of mental health services in each area. The ways of developing our mental health system have been suggested. Key Words : mental health strategy, deinstitutionalization, community mental health, team approach