정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

Influence of Bundle Diameter and Attachment Point on Kinematic Behavior in Double Bundle Anterior Cruciate Ligament Reconstruction Using Computational Model

Kwon, Oh Soo,Purevsuren, Tserenchimed,Kim, Kyungsoo,Park, Won Man,Kwon, Tae-Kyu,Kim, Yoon Hyuk Hindawi Publishing Corporation 2014 Computational and mathematical methods in medicine Vol.2014 No.-

<P>A protocol to choose the graft diameter attachment point of each bundle has not yet been determined since they are usually dependent on a surgeon's preference. Therefore, the influence of bundle diameters and attachment points on the kinematics of the knee joint needs to be quantitatively analyzed. A three-dimensional knee model was reconstructed with computed tomography images of a 26-year-old man. Based on the model, models of double bundle anterior cruciate ligament (ACL) reconstruction were developed. The anterior tibial translations for the anterior drawer test and the internal tibial rotation for the pivot shift test were investigated according to variation of bundle diameters and attachment points. For the model in this study, the knee kinematics after the double bundle ACL reconstruction were dependent on the attachment point and not much influenced by the bundle diameter although larger sized anterior-medial bundles provided increased stability in the knee joint. Therefore, in the clinical setting, the bundle attachment point needs to be considered prior to the bundle diameter, and the current selection method of graft diameters for both bundles appears justified.</P>

Effects of Interleukin-1β, Tumor Necrosis Factor-α and Interferon-γ on the Nitric Oxide Production and Osteoclast Generation in the Culture of Mouse Bone Marrow Cells

Kwon, Young-Man,Kim, Se-Won,Ko, Seon-Yle The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of 30μM SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration(3μM) of SNP enhanced it. Althogh the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone matabolism and bone pathophysiology.

연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

1RM 적용에 따른 강도별 웨이트트레이닝 운동이 혈역학 반응과 심근 부담율에 미치는 영향

권만근(Kwon, Man-Geun),김영주(Kim, Young-Joo) 한국체육과학회 2017 한국체육과학회지 Vol.26 No.1

The purpose of this study is to investigate the effect of weight exercise on physiological response according to weight by comparing the hemodynamic response and rate pressure product (RPP) according to weight exercise of each area at 60% and 80% of 1RM. Treadmill and 1RM tests were conducted in healthy 20’s men. Also, the ratio of bench press (BP), deadlift (DL), military press (MP), squat (SQ), and behind press (BNP) at 6 0 and 80% of 1RM according to HR, SBP, RPP, MRPP was measured. At 60% o f 1RM in HR, DL, MP, SQ, and BNP were significantly higher than BP (p<.001, p<.01, p<.001), and SQ was significantly higher than MP(p<.05). In SBP, DL was significantly higher than BP and MP(p<.01, p<.05), and SQ was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.01). In RPP, BP was significantly lower than DL, SQ, and BNP(p<.001, p<.001, p<.05), and DL was significantly higher than MP(p<.001). SQ was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.05). In the ratio to MRPP reach, DL was significantly higher than BP(p<.01), and SQ was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.01). At 80% of 1RM in HR, B P was significantly lower than D L, M P, SQ, a nd BNP(p<.001, p<.001, p<.001, p<.01), and SQ was significantly higher than BNP(p<.05). In SBP, DL and SQ was significantly higher than BP, MP, BNP(p<.001). In RPP, DL was significantly higher than BP, MP, and BNP(p<.001, p<.001, p<.01), and SQ was sigficantly higher than BP, MP, and BNP(p<.001). At MRPP reach ratio, DL was significantly higher than BP, MP, and BNP(p<.001, p<.01, p<.01), and SQ was significantly higher than BP, MP, and BNP(p<.001). In conclusion, when applying 60% and 80% of 1RM of each weight exercise, RPP was clealy increased according to high blood pressure response in DL and SQ, and there was lower heart rate compared with other exercise type in BP. The safe exercise prescription should be necessary when applying weight exercise for patients who need cautions of weight exercise.

두통을 주소증으로 하여 입원한 환자 120예에 대한 임상적 고찰

권경만 ( Kyoung Man Kwon ),김광록 ( Koang Lok Kim ),신윤리 ( Yoon Ri Shin ),박선영 ( Sun Young Park ),박동일 ( Dong Il Park ) 경락경혈학회 2012 Korean Journal of Acupuncture Vol.29 No.4

Objectives: The Purpose of this study is to investigate clinical characteristics with 120 patients who have sufferred from headache and were treated. Methods: We classified 120 patients into several groups by International Headache Society(IHS) classification and evaluated the effects of oriental medical therapy on headache. Results: 1. According to the statistics, women patients were more than men. And 50s has highest number of headache patients. 2. In classification by IHS, tension type headache were highest. 3. 24.2% of patients had entered Department of Internal Medicine, Colleage of Oriental Medicine Dongeui Univ for treatment within six month of onset. 4. According to the statistics, headache of whole part has highest number of headache patients. 5. The most number of associated symptoms were nausea, vomiting, dyspepsia etc. 6. The most number of headache patient`s past history were hypertension. 7. In classification by oriental medical differentiation of symptom and signs, the rate of blood deficiency were highest. 8. 64.2% of patients said they were satisfied with treatment, reporting half the frequency of headache or better from before treatment. Conclusions: In conclusion, the present results suggest that oriental medical therapy has effects on headache.

Clinical Characteristics and Treatment Outcomes of Chronic Pulmonary Aspergillosis: a Retrospective Analysis of 70 Patients

( Byung Woo Jhun ),( Kyeong Man Jeon ),( Jung Seop Eom ),( Ji Hyun Lee,),( Gee Young Suh ),( Man Pyo Chung ),( O Jung Kwon ),( Won Jung Koh ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Backgroud: Chronic pulmonary aspergillosis (CPA) is uncommon and there are few data on the clinical characteristics, optimal therapeutic strategies, and outcomes. Methods: We retrospectively reviewed the records of patients with newly diagnosed CPA and assessed the clinical, radiologic, and laboratory responses, and outcomes between January 2008 and January 2012. Results: A total of 70 CPA patients were included. Median age was 55 years and 51 patients (72.9% ) were men. Fifty-seven patients (81.4%) had a history of pulmonary tuberculosis and 32 patients (45.7%) had non-tuberculosis mycobacterium (NTM) lung disease, 17 of whom had a history of NTM treatment and 15 was under NTM treatment. Sixty-nine patients (98.6%) received oral itraconazole, except one with oral voriconazole for a median duration of 6.2 months. Symptomatic improvement occurred in 52 patients (74.3%), radiologic improvement occurred in 29 (41.4%), and laboratory tests improved more than 60% of all patients. Thirty-six patients (51.4%) achieved treatment success, defined as symptomatic improvement after at least 6 months of therapy regardless of radiological improvement, and 4 of whom needed retreatment after a median of 8.4 months. There was no difference in clinical characteristics and outcomes according to NTM status and 10 (14.3%) discontinued therapy due to adverse reactions. Death occurred in 10 (14.3%) and median follow-up duration was 11.2 months. Conclusion: Antifungal therapy for about 6 months and reassessment of disease might be feasible treatment strategy in managing patients with CPA.

2011 대구세계육상선수권대회 남자 높이뛰기 메달리스트들의 바이오메카닉스적 특성 분석

김의환 ( Eui Hwan Kim ),배영상 ( Young Sang Bae ),김성섭 ( Sung Sup Kim ),권문석 ( Moon Seok Kwon ),위웅량 ( Ung Ryang Wo ),김기만 ( Ki Man Kim ),이정민 ( Jeong Min Lee ) 한국운동역학회 2011 한국운동역학회지 Vol.21 No.5

The purpose of this study was to perform a kinematic analysis of the high jump techniques of the three men`s medalists at the 2011 IAAF Championships in Daegu (August 27-September 4, 2011), In particular, a three-dimensional coordinates method was used to analyze the last three strides before touchdown, the touchdown techniques, and the movements after takeoff toward the bar. An analysis of the, data for the biomechanical characteristics of the world`s best high jumpers could contribute to an improvement in the performance of a national high jumper. The first conclusion of the data analysis was that the arm movements of the gold medalist, J. Williams, had a single arm form, whereas the arm movements of the other medalists were a double arm form. Second, the difference in the knee joint angles upon touchdown and toe-off was 10˚. Third, J. Williams achieved his maximum CM height after takeoff (1.26m) using the maximum flexion of his knee joint. Fourth, the foot contact duration of A. Dmitrik (0.11 s) was the shortest among the medalists, and the ratio for his transformation of horizontal velocity to vertical velocity was the greatest (75.25%) among the three. Last, the maximum CM height of T. Barry was the greatest, and his foot contact duration was the longest.

중고령자에 있어서 적절한 운동처방의 연구

이배익,정일호,권만근 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.4

Recently, the diet of middle aged Korean men is changing. Instead of eating Korean foods exclusively, they are consuming more Western foods which are often unhealthy. In addition, they are exercising less. These factors are leading to a rise in lifestyle related disease, such as diabetes, and dangerous medical conditions like hypertension. As a result, some middle aged men have taken up light exercise, such as hiking, walking, swimming etc. However, they don't know how to set up an exercise program, i.e. frequency of exercise, intensity, duration, etc. A 15 week exercise class was set up 36 middle aged men. Before the class began, a physical fitness and blood test were administered to each subject. The same tests were repeated after the 15th week. Based on the initial results, individual programs were prepared for each participant. A step calorie counter was supplied to measure their exercise activity. The step count results were checked everday, and based on the results, the subjects were divided into three groups for analytical purposes. The firts group, sixteen subjects(44%), averaged a little over 8,000 steps per day. The second group, eight subjects(22%), averaged between 8-10,000 steps per day. The third group, twelve subjects(33%), averaged a little over 10,000 steps per day. After fifteen weeks, the total cholesterol, HDL cholesterol and LDL cholesterol of all subjects showed no significant change. The second and third group showing an especially marked decrease in blood sugar. Also, physical fitness improved for the second and third groups. In conclusion, both groups doing the most exercise showed improvement in their TG and blood sugar results. Blood pressure also decreased for these two groups, which was not true for the first group, also, physical fitness improved in the second and third groups, while the first group showed no significant change. It appears that exceeding a certain minimum level of exercise is required to achieve good results. What would happen if group 1 did the same exercise for longer than is weeks.

초음파 기관지 내시경으로 진단한 기관기관지 골연골형성증 1예

이천우,옥철호,정만홍,장태원,임성경,조은주,이신준,이혜원,구상건,천봉권 고신대학교 의과대학 2011 고신대학교 의과대학 학술지 Vol.26 No.2

Tracheobronchopathia osteochondroplastica (TO) is a rare benign disease of unknown etiology characterized by accumulation of calcium phosphate in the submucosa of large airways and benign proliferation of bone and cartilage resulting in nodular formation. We report a case of tracheobronchopathia osteochondroplastica diagnosed by Endobronchial ultrasonography in a 56-year-old man. Chest Computed Tomography revealed thickening of tracheal and bronchial wall, and multiple nodules through whole trachea. Endobronchial ultrasonography showed numerous submucosal nodules with hetero-echogenecity in the third and fourth layers. Histopathological examination revealed nonspecific bronchitis with squamous metaplasia and metaplastic ossification. We confirmed tracheobronchopathia osteochondroplastica. The patient's symptoms were successfully treated with antibiotics and oxygen supplyment. endobronchial ultrasonography can helpful diagnosis in tracheobronchopathia osteochondroplastica.