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Bio Clean Room(BCR)의 멸균을 위한 산소 클러스터이온 발생 장치 개발에 관한 연구
박동일(Dong-il Park),정광섭(Kwang-Seop Chung),김영일(Young-Il Kim),김성민(Sung-Min Kim) 대한설비공학회 2013 설비공학 논문집 Vol.25 No.1
Bio Clean Room(BCR) and pharmaceutical product manufacturing facilities require careful assessment of many factors, including HVAC, controls, room finishes, process equipment, room operations, and utilities. Flow of equipment, personnel, and product must also be considered along with system flexibility, redundancy, and maintenance shutdown strategies. It is important to involve designers, operators, commissioning staff, quality control, maintenance, constructors, validation personnel, and the production representative during the conceptual stage of design. Critical variables for room environment and types of controls vary greatly with the clean space’s intended purpose. It is particularly important to determine critical parameters with quality assurance to set limits and safety factors for temperature, humidity, room pressure, and other control requirements. In this paper, oxygen cluster ion equipment was utilized in order to enhance the indoor air quality and to prevent the airborne infection of ward in hospital. Moreover, the performance test of the equipment was also performed in order to develop the optimal sterilization system of BCR using the equipment.
Mongolian gerbil에서 Helicobacter pylori 감염 후 발생되는 Microsatellite Instability에 관한 연구
박동일 ( Dong Il Park ),김민경 ( Min Kyung Kim ),김재준 ( Jae J. Kim ),유태우 ( Tae Woo Yoo ),박정호 ( Jung Ho Park ),김홍주 ( Hong Joo Kim ),조용균 ( Yong Kyun Cho ),손정일 ( Chung Il Sohn ),전우규 ( Woo Kyu Jeon ),김병익 ( Byung 대한내과학회 2006 대한내과학회지 Vol.71 No.1
목적: H. pylori (HP) 감염성 위염에서 HP는 DNA 손상복구기능에 장애를 유발하여 위 상피세포의 유전자에 발생하는 돌연변이를 축적시켜 위암의 발생 위험을 증가시킬 수 있다. 본 연구는 Mongolian gerbil (MG)animal model을 이용한 in vivo 실험에서 만성적인 HP감염이 위 점막의 MSI를 발생시키는 지 알아보고자 하였다. 방법: 7주된 수컷 MG에 HP CA20 균주를 감염시킨 후 일정한 시점마다 동물을 희생시켜 위를 절제하였다. 위염부위의 위 상피를 미세박리하여 DNA를 추출한 후 Fam이나 Hex로 표지시킨 다섯 가지 gerbil MSI marker를 이용하여 중합효소연쇄반응을 실시한 후 310 genetic analyzer를 이용하여 MSI 분석을 시행하였다. 결과: MSI는 정상부위(식도)에서는 관찰되지 않았고, 위염이 없는 부위에 비해 위염부위에서 빈번히 관찰되었다(p<0.05). 위염부위 중 6예에서는 low level MSI를 1예에서는 high level MSI를 관찰하였다(p<0.05). 결론: 본 연구로 MG에서 만성적인 HP 감염이 MSI유발에 관여함을 입증하였다. 이와 같이 HP는 위상피세포내의 DNA mismatch repair 단백 발현을 저하시켜 위상피세포 유전자에 발생하는 돌연변이의 축적을 유발할 수 있을 것으로 생각한다. Background: During Helicobacter pylori (HP) gastritis, the organisms cause an impairment of DNA repair that results in accumulation of mutations in the genome of epithelial cells and an increased risk of gastric carcinoma. The aim of this study was to determine whether microsatellite instability (MSI) could be induced by chronic HP infection in the Mongolian gerbil model. Methods: Seven-weeks old, specific pathogen-free male Mongolian gerbils were infected with the HP strain CA20. They were sacrificed at the each time points (1, 2, 3, 6, 9, 12 months). Areas of gastritis were carefully microdissected. DNA from tissues was analyzed for the presence of MSI by genomic DNA amplification with five gerbil microsatellite markers per sample. Gerbil markers labelled with Fam or Hex were obtained to perform MSI analysis using the 310 Genetic Analyzer. Results: MSI was more frequent in the area of gastritis than in that of normal (p<0.05). Six showed MSI-low and one showed MSI-high within the area of gastritis (p<0.05). Conclusions: This study shows that chronic HP infection in the Mongolian gerbil induces MSI. This finding indicates that HP infection causes a decrease in DNA MMR proteins in epithelial cells of the stomach that may reach critically low levels, allowing for the accumulation of mutations such as those seen in microsatellite regions.(Korean J Med 71:17-23, 2006)
박동일(Dong Il Park),임성균(Sung-Kyun Lim),곽윤근(Yoon Keun Kwak) 대한기계학회 2005 대한기계학회 춘추학술대회 Vol.2005 No.5
There have been many researches about the driving mechanism for off-road mobility and the various utilities of rescue robot constantly. In this paper, a new type of driving mechanism for rescue robot which has a variable geometry single-track is introduced to achieve such objectives as surveillance, reconnaissance and rescue activity. It was designed to improve the performance of overcoming stairs and energy efficiency and to minimize its weight and size. We analyzed the design parameters for several steps of climbing stairs and confirm the possibility of realization of presented driving mechanism from various experiments.