Evaluation of Combined Quantification of PCA3 and AMACR Gene Expression for Molecular Diagnosis of Prostate Cancer in Moroccan Patients by RT-qPCR

Maane, Imane Abdellaoui,El Hadi, Hicham,Qmichou, Zineb,Al Bouzidi, Abderrahmane,Bakri, Youssef,Sefrioui, Hassan,Dakka, Nadia,Moumen, Abdeladim Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.12

Prostate cancer (PCa) remains one of the most widespread and perplexing of all human malignancies. Assessment of gene expression is thought to have an important impact on cancer diagnosis, prognosis and therapeutic decisions. In this context, we explored combined expression of PCa related target genes AMACR and PCA3 in 126 formalin fixed paraffin embedded prostate tissues (FFPE) from Moroccan patients, using quantitative real time reverse transcription-PCR (RT-qPCR). This quantification required data normalization accomplished using stably expressed reference genes (RGs). A panel of twelve RG was assessed, data being analyzed using GenEx V6 based on geNorm, NormFinder and statistical methods. Accordingly, the hnRNP A1 gene was identified and selected as the most stably expressed RG for reliable and accurate gene expression quantification in prostate tissues. The ratios of both PCA3 and AMACR gene expression relative to that of the hnRNP A1 gene were calculated and the performance of each target gene for PCa diagnosis was evaluated using receiver-operating characteristics. PCA3 and AMACR mRNA quantification based on RT-qPCR may prove useful in PCa diagnosis. Of particular interesting, combining PCA3 and AMACR quantification improved PCa prediction by increasing sensitivity with retention of good specificity.

흰쥐 뇌파에 미치는 Halothane 마취 효과의 정량적 분석

박성근,이만기 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.2

An objective quantification of the level of halothane anesthesia by spectrum analysis of EEG recorded from the rat scalp was studied. The rats were divided into four groups according to exposure concentration of 1.0, 1.5, 2.0 and 2.5% halothane, and then bipolar EEG recording through one lead from the rat scalp was followed by power spectrum analysis. The density of each band (delta 1-3.5 Hz, theta 3.5-8 Hz, alpha 8-13 Hz, betal 13-18 Hz, beta2 18-21 Hz, and beta3 21-30 Hz) and total density, and median power frequency were calculated from the spectrums. These 8 parameters were taken for a stepwise or direct discriminant analysis. Predicted groups by the discriminant analysis for the EEG parameters were in accordance with 94.12% of the actual groups. The results suggest that the discriminant analysis for the EEG parameters derived from power spectrum can be applied to determination on the level of halothane anesthesia.

Acetylcholine에 의한 적출 십이지장 평활근 수축에 미치는 Calcium 길항제의 영향

손의동,이만기,박창호,김인겸,김중영 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.2

To compare antagonistic action of caloium antagonists on intestinal smooth muscle contraction, acetylcholine-induced phasic contraction (PC) and tonic contraction (TC) initiated by different calcium utilization were observed in the presence of various calcium antagonists by the use of isolated mouse duodenum suspended in Tyrode's solution. By verapamil at 2.2×10 exp(-6), 2.2×10 exp(-5) and 2.2×10 exp(-4) mM, PC was more decreased dose-dependently than TC. By nifedipine at 7.9×10 exp(-5) mM, PC was more decreased than TC, but at 7.9×10 exp(-6) mM it did not affect PC and TC. By diltiazem at 6.1×10 exp(-4) mM, PC was more decreased than TC, but at 2.4×10 exp(-4) and 2.4×10 exp(-5) mM it did not affect both of the contractions. By cobalt chloride at 0.3, 1.3, and 2.6 mM, TC was significantly decreased without affecting PC, but PC markedly decreased at 5.2 mM. These results reconfirmed the fact that cobalt ion-induced calcium antagonism is more related to calcium influx process than calcium release process in contrast with the actions of verapamil, diltiazem, and nifedipine in duodenal smooth muscle.

EFFECTS OF LIDOCAINE ON SOMATOSENSORY EVOKED POTENTIALS IN RAT VIBRISSA/BARREL CORTEX

남순현,최병주,김현정,이만기,김영진,이혜숙 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

본 실험은 삼차신경 자극으로 발생되는 체성 감각 유발 전위에 대한 국소마취제의 효과를 관찰하였다. 나트륨 통로차단을 통하여 약리작용을 나타내는 것으로 알려져 있는 리도카인를 뇌 피질에 국소 투여한 후 삼차신경의 체성 감각유발 전위의 강도및 지연시간을 측정하였다. 케타민으로 마취된 흰쥐의 대측성 구레나룻 자극후 뇌의 체성 감각영역으로부터 기록되는 유발전위를 분석한 결과, 리도카인을 뇌 피질에 국소 투여시 유발전위의 강도 및 지연시간의 감소가 나타났으며, 필드 전위의 형태는 이상성(양극성 및 음극성) 혹은 삼상성(양극성, 음극성 및 양극성)의 파형으로 나타났다. 필드 전위의 발생 부위는 뇌 피질의 중대뇌동맥의 상행지 상방영역이었다. 본 실험에서 나타난 초기 전위변동은 피질판 상층에 존재하는 신경세포의 탈분극 과정에 의하여 생성되고 후기 전위 변동은 피질판 상층에 존재하는 신경세포의 탈분극 과정에 의하여 생성되고 후기의 전위 변동은 동일 영역의 하층 신경세포에서 과분극 혹은 재분극이 발생한 결과라고 유추된다. 따라서 삼차신경계의 체성 감각 영역에서는 피질 상층및 하층의 과립성 피라미드 세포의 순차적인 활성화에 의하여 기본적인 신경 회로망이 형성되어 있으며 생리적 자극으로 유발되는 필드 전위는 이러한 신경망를 통하여 발생될 것으로 사료된다.

기관지 방선균증의 임상 1례

이은영,장태원,정만홍,옥철호,이규원,손창배,이지숙,신은경,서정아,백종현,김영옥 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Actinomycosis is a chronic suppurative infection, whitch infiltrates mucosa-associated tissues. Thoracic, pelvic and cervicofacial infections of actinomyces are not uncommon, but endobronchial actinomycosis is rarely reported. We report a case of a 29 year old man who presented with a recurrent pneumonia and chronic cough. Fiberoptic bronchoscopy revealed occlusion of the right. lower medio-basal segment bronchus with exophytic endobronchial mass. The diagnosis was confirmed histologically by demonstration of sulfur granules in the bronchoscopic biopsy of the mass. Intravenous administration of amoxacillin and clindamycin followed by oral amoxacillin and clindamycin therapy resulted in complete recovery.

백서에서 Chlorpromzaine,Haloperidol,Clozapine이 뇌파에 미치는 영향

정성훈,박영남,이만기,박진숙 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

To evaluate the differential elects of chlorpromazine, haloperidol, and clozapine on electroencephalographic (EEG) changes, EEG findings were compared in normal, relaxant, and schizophrenic animal models produced with MK-801 by using power spectral EEG analysis. Under pentobarbital anesthesia, the screw electrodes were implanted on the skull over the frontal cortex for EEG recording. The EEG was recorded before and after intraperitoneal administration of clozapine(5mg/kg), chlorpromazine(5mg/kg) and haloperidol(1mg/kg) in rats while awake(the normal group), rats pretreated with a relaxant pancuronium bromide(the relaxant group) and rats pretreated wish MK-801(0.2mg/kg, i.p., the MK-801 group). Spectrum analysis of the EEG was used to calculate the powers of each band (delta 1-4Hz; theta 4-8Hz; alpha 8-l3Hz; beta L 13-21Hz; beta H 21-30Hz; gamma 30-50Hz; and total 1-50Hz). The EEG activity was presented as the log value of the relative band power. In the relaxant group, clozapine increased the activity of the delta, theta and alpha bands. Chlorpromazine increased the activity of the betaL band as well as the delta, theta and alpha bands and it decreased the activity of the gamma band. However, haloperidol did not change the activity of all bands significantly. In the MK-801 group, clozapine increased the activity of the delta and gamma bands and chlorpromazine increased the activity of the delta, betah and gamma bands significantly, Haloperidol increased the activity of the alpha and betaL bands significantly. To evaluate the differential effects of antipsychotics on EEG between the groups, the ratio of power change after a drug as referenced to pre-drug power in each group was calculated and compared between the groups and was represented by star-like display. In the star-like display, clozapine showed the smallest change and caused a slight shift upwards and to the left, chlorpromazine showed irregular changes, haloperidol showed similar increases along each band.

수종 자궁수축제의 마우스의 적출자궁 수축력에 미치는 효과의 비교

이상엽,주영은,이만기 啓明大學校 醫科大學 1983 계명의대학술지 Vol.2 No.1

마우스 비임신적출자궁 5예와 임신전기적출자궁 6예에 자궁수축제 oxytocin, PGF, ergonovine maleate를 투여하여 다음 결과를 얻었다. 비임신자궁에서 oxytocin과 PGF는 ergonovine maleate보다 가가 23배, 26배의 효능을 가졌고, oxytocin은 PGF와 ergonovine maleate보다 약 126배, 145배의 효력을 가졌다. 임신전기자궁에서 oxytocin과 PGF는 ergonovine maleate보다 각각 1.6배, 1.8배의 효능을 가졌고, oxytocin은 PGF보다 약 9배, ergonovine maleate보다 약 5배의 효력을 가졌다. Oxytocin은 임신자궁보다 비임신자궁에 효력이 강하나, PGF와 ergonovine maleate는 비임신자궁보다 임신자궁에 효력이 강하게 나타났다. The response of the isolated uterus from pregnant (gestational period less than 10 days) and nonpregnant mouse to uterine stimulants, oxytocin, prostaglandin F₂α (PGF₂α )and ergonovine, was observed in low calcium Tyrode's solution. Potency was represented by the value of pD₂ which is defined as the negative logarithm of the concentration producting half- maximal response to each drug. In the non-pregnant uterus, the pD₂ values of oxytocin, PGF₂α, and ergonovine were 8.86, 6.76, and 6.70, respectively. In the pregnant uterus, the values were 8.45, 7.50, and 7.75, respectively. A maximal response produced by oxytocin was similar to that by PGF₂α in both pregnant and nonpregnant uterus. However, the maximal response to ergonovine in the nonpregnant uterus was one-twenty fifth the response to oxytocin and PGF₂α, and in the pregnant uterus it was a half the response to them.

기관지 탄분 섬유화증 환자의 임상 경과와 고감도 C-반응 단백(high sensitivity C-reactive protein, hsCRP)과의 상관 관계 비교

이규원,옥철호,장태원,정만홍 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Background: To investigate the association between bronchial anthracofibrosis(AF) and tuberculosis(TB). and the clinical utility of high sensitivity C-reactive protein(hsCRP) for diagnosis of active pulmonary TB in patients with bronchial AF. Method: Eighty three patients(62 women and 21 men ranging in age from 55 to 87), who were diagnosed with bronchial AF by a bronchoscopy, were enrolled in this study. The hsCRP was measured in patient's blood. The hsCRP results were compared with the bacteriological, and clinical finding. Results: The common respiratory symptoms were cough(70%), sputum(35%), dyspnea(20%), and hemoptysis(10%). The sputum AFB smear, culture were positive in 20(22.8%) and 37(44.5%) patients, respectively. The level of hsCRP in patients with postive culture was significantly higher than that of patients with negative culture.(4.67±7.5, 0.90±1.59, p=0.005). Of 63 patients with negative smear, the level of hsCRP in patients(n=18) with positive culture was higher than that of patients(n=45) with negative culture.(3.39±5.78, 0.92±1.50, p=0.013). Of 20 patients with negative smear and positive culture who were empirically treated with anti-TB medication, 11 patients showed radiologic response. and their level of hsCRP was higher than that of 9 patients who showed no radiologic response.(1.56±1.89, 0.81±0.10,p=0.03) Conclusion: hsCRP appears to be useful diagnostic tool for predicting active pulmonary tuberculosis. in patients with bronchial AF.

Methylphenidate 투약에 따른 주의력결핍 과잉운동장애 아동의 정량화 뇌파의 변화

이병대,정성훈,이만기,장수민,백지령,임효덕 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.1

Fogarty Catheter를 이용한 소아 기도 이물의 제거 1례 : 증례 보고

김예나,옥철호,장태원,정만홍 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Foreign body aspiration is a common problem in children necessitating prompt recognition and early treatment to minimize the potentially serious and sometimes even fatal conseqeunce. We report the case of a 7-year-old boy with a button lodged in his right main bronchus. Under general anesthesia, ultrathin bronchoscopy identified the foreign body which was then removed by the combined use of a Fogarty catheter and extraction forcep. This case differs from previous reports, in that the forcep extraction through working channel of ultrathin scope was used simultaneously with Fogarty catheter even in relately small airway of children.