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- 저자 : Li Defeng (검색결과 10 건)
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Defeng Li,Jinling Fan,Lin Du,Guoyan Ren 대한약학회 2024 Archives of Pharmacal Research Vol.47 No.2
Insulin resistance (IR) is a key factor in the pathogenesis of disrupted glucose metabolism. Although the extract of Glycyrrhizaglabra has shown signifi cant hypoglycemic activity, its bioactive components remain to be identifi ed, and theirmechanisms of action, especially on hepatocyte glucose metabolism, are yet to be explored. In the present study, the primarycompounds from Glycyrrhiza glabra [named prenylated fl avonoid fractions (PFFs)] have been identifi ed and their chemicalstructures have been elucidated. The therapeutic eff ects of PFFs extracted from G. glabra on glucose metabolism disordersand IR in high insulin-induced insulin-resistant HepG2 (IR-HepG2) cells have been determined. Glabridin (GLD) was usedas a control. The results indicated that, similar to GLD, PFFs increased glucose consumption, glucose uptake, and translocationof glucose transporter 4 to the plasma membrane in IR-HepG2 cells. In addition, they enhanced the activities ofglycogen synthase, glucokinase, and pyruvate kinase, while reducing the activities of phosphoenolpyruvate carboxykinaseand glucose-6-phosphatase. Furthermore, they activated the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt)pathway and suppressed the extracellular signal-regulated kinase/insulin receptor substrate-1 (ERK/IRS-1) pathway. Thesefi ndings suggest that, similar to GLD, PFFs can alleviate impaired glucose metabolism and alleviate IR in IR-HepG2 cells.
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Defeng Guan,Chenyang Li,Xiao Lv,Yongxiu Yang 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.5
Objectives: Pseudolaric acid B (PAB) has been shown to inhibit the growth of various tumor cells, but the molecular details of its function are still unknown. This study investigated the molecular mechanisms by which PAB induces apoptosis in HeLa cells. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to investigate the effect of PAB treatment in various cervical cancer cell lines. Annexin V/propidium iodide staining combined with flow cytometry and Hoechst 33258 staining were used to assess PAB-induced apoptosis. Additionally, we performed bioinformatics analyses and identified a paired box 2 (PAX2) binding site on the BAX promoter. We then validated the binding using luciferase and chromatin immunoprecipitation assays. Finally, western blotting assays were used to investigate PAB effect on the Wnt signaling and the involved signaling molecules. Results: PAB promotes apoptosis and downregulates PAX2 expression in HeLa cells in a time- and concentration-dependent manner. PAX2 binds to the promoter of BAX and inhibits its expression; therefore, PAX2 inhibition is associated with increased levels of BAX, which induces apoptosis of HeLa cells via the mitochondrial pathway. Additionally, PAB inhibits classical Wnt signaling. Conclusion: PAB effectively inhibits Wnt signaling and PAX2 expression, and increases BAX levels, which induce apoptosis in HeLa cells. Therefore, PAB is a promising natural molecule for the treatment of cervical cancer.
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Baoyue Li,Yonghua Yu,Jia Hu,Bingxin Cao,Yangfeng Yao,Defeng Xu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12
The vibration signal of rotating mechanical equipment contains a large amount of information that can be used for the fault diagnosis of rotating mechanical equipment. However, the vibration information is distributed in multiple dimensions, and a single-scale analysis cannot effectively reflect its damage characteristics, reducing the accuracy of fault diagnosis. Accordingly, an improved hierarchical fluctuation dispersion entropy (MHFDE) method based on the improved hierarchical processing is proposed. MHFDE can simultaneously mine low- and highfrequency features in the time series, avoiding information omission. Comparison results of the simulated signals show that the proposed method has the advantages of high stability and accurate measurement of complexity. In combination with the multi-cluster feature selection (MCFS) and kernel limit learning machine (KELM) optimized by whale optimization algorithm (WOA), a rotating machinery damage recognition method based on MHFDE-MCFS and WOA-KEM was proposed. Three sets of typical rotating machinery datasets are used to verify the effectiveness of the proposed method. The results show that this method can not only accurately and stably identify the damage types of the three selected machinery but also have a higher accuracy of damage recognition compared with the existing feature extraction methods.
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Settlement behaviours and control measures of twin-tube curved buildings-crossing shield tunnel
Jianwei Jia,Ruiqi Gao,Defeng Wang,Jianjun Li,Ziwen Song,Jinghui Tan 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.84 No.5
Settlement control techniques are critical for the safety of shield tunnel constructions, especially for facing complex situations. In this study, the shield tunnel structure from Huaita east road station to Heping Road station in Xuzhou metro No.3 line (China) is taken as engineering background, which has various complex problems of the upper-soft and lower-hard composite stratum conditions, twin curve shield tunnels, and underpass the foundation of the piled raft. The deformation characteristics of shield tunnelling passing through buildings are explored. Subsequently, comprehensive research methods of numerical simulation and field measurement are adopted to analyzing the effectiveness of settlement control by using the top grouting technique. The results show that the settlement of the buildings has obvious spatial characteristics, and the hysteresis effect can be obviously observed in soil deformation caused by shield construction. Meanwhile, the two shield constructions can cause repeated disturbances, reducing the soil deformation's hysteresis effect. Moreover, the shield tunnel's differential settlement is too large when a single line passes through, and the shield construction of the outer curve can cause more significant disturbance in the tunnel than the inside curve. Notably, the proposed process control parameters and secondary topgrouting method can effectively control the deformation of the shield tunnel, especially for the long-term deformation.
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( Qianqian Zhang ),( Xiehao Wang ),( Defeng Zhang ),( Meng Long ),( Zhenbing Wu ),( Yuqing Feng ),( Jingwen Hao ),( Shuyi Wang ),( Qian Liao ),( Aihua Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8
Fish mycobacteriosis is a common bacterial disease in many species of freshwater and marine fish and has caused severe loss of fish production. Mycobacterium marinum has been the most prevalent pathogen observed in several outbreaks of mycobacteriosis of farmed sturgeons in China. However, the immune responses and pathology of sturgeons in mycobacterial infection are rarely studied. Therefore, we used the Illumina RNA-seq method to analyze the transcriptome profile of Acipenser schrenckii challenged with Mycobacterium marinum. To begin, 168,220 non-redundant contigs were acquired from the infection and control groups, and among these, 33,225 contigs have acquired annotations. A total of 4,043 differently expressed (DE) contigs between the two groups were identified, and among these, 2479 were upregulated and 1564 were down-regulated in the infected fish. A total of 1,340 DE contigs with acquired annotations in KEGG were enriched for 124 pathways including the TNF signaling pathway, and the Toll-like receptor signaling pathway. The roles of DE genes involved in significant pathways and other processes were discussed. The 2,209 DE contigs that have yet to acquire proper annotation may represent candidate genes associated with infection in sturgeons and are expected to serve as immunogenetic resources for further study. To our best knowledge, this is the first transcriptome study on sturgeons under bacterial infection.
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Huilai Liu,Minshu Cui,Yao Liu,Defeng Kong,Zhihao Li,Rohan Weerasooriya,Xing Chen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-
Sulfamethoxazole (SMX) is a widely used antibiotic drug in medical applications resulting in its ubiquityin water and wastewater. Most of the treatment processes are inefficient in destroying SMX in water. Theheterogeneous electro-Fenton (EF)process shows promise in the non-selective degradation of antibioticdrugs in water and wastewater. Fe2O3/MXene-x (x is the ferric ion loading) was fabricated on 2DMXene carriers by hydrothermal method. The Fe2O3/MXene-x substrates were characterized by electronmicroscopic, X-ray, and molecular spectroscopic methods. Degradative products of SMX were identifiedby free radical quenching and LC-MS measurements. Electron micrographs show that Fe2O3 particulatesare uniformly distributed on MXene nanosheets. Fe2O3/MXene-x coated graphite was used as a cathodewith titanium anode for in situ generation of H2O2 required to produce free radicals (e.g. OH and O2 – ) todestroy SMX. Three possible SMX degradation routes by the electro-Fenton process were proposed. SMXand intermediates are eventually mineralized as CO2 and H2O. The new electrocatalyst shows reducedmetal ions leaching and good stability in repeated use cycles. This work offers a new electro-Fentonmethod to destroy antibiotic drugs in water and wastewater.
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Experimental study on wave motion of partial air cushion supported catamaran
Jinglei Yang,Hanbing Sun,Xiaowen Li,Defeng Wu 대한조선학회 2023 International Journal of Naval Architecture and Oc Vol.15 No.-
To study the performance of partial air cushion-supported catamaran (PACSCAT), which is sailing with a flexible air seal in waves, this paper uses the model tests, and research the motion characteristics of PACSCAT at different wavelengths, by monitoring the boundaries of heave, pitch, acceleration, air cushion pressure. Firstly this paper introduces the test situation of PACSCAT in the towing tank and introduces the test method, equipment, and test conditions. Second, this paper extracts the test data, analyzes the features of heave, pitch, acceleration, and air cushion pressure, and studies their linear and nonlinear changes in regular waves. Thirdly this paper analyzes the nonlinear air leakage flow of the bow of PACSCAT in detail under the test conditions and studies the internal and external free liquid surface under different conditions.
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Microbial short-chain fatty acids: a bridge between dietary fibers and poultry gut health — A review
Ali Qasim,Ma Sen,La Shaokai,Guo Zhiguo,Liu Boshuai,Gao Zimin,Farooq Umar,Wang Zhichang,Zhu Xiaoyan,Cui Yalei,Li Defeng,Shi Yinghua 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10
The maintenance of poultry gut health is complex depending on the intricate balance among diet, the commensal microbiota, and the mucosa, including the gut epithelium and the superimposing mucus layer. Changes in microflora composition and abundance can confer beneficial or detrimental effects on fowl. Antibiotics have devastating impacts on altering the landscape of gut microbiota, which further leads to antibiotic resistance or spread the pathogenic populations. By eliciting the landscape of gut microbiota, strategies should be made to break down the regulatory signals of pathogenic bacteria. The optional strategy of conferring dietary fibers (DFs) can be used to counterbalance the gut microbiota. DFs are the non-starch carbohydrates indigestible by host endogenous enzymes but can be fermented by symbiotic microbiota to produce shortchain fatty acids (SCFAs). This is one of the primary modes through which the gut microbiota interacts and communicate with the host. The majority of SCFAs are produced in the large intestine (particularly in the caecum), where they are taken up by the enterocytes or transported through portal vein circulation into the bloodstream. Recent shreds of evidence have elucidated that SCFAs affect the gut and modulate the tissues and organs either by activating G-protein-coupled receptors or affecting epigenetic modifications in the genome through inducing histone acetylase activities and inhibiting histone deacetylases. Thus, in this way, SCFAs vastly influence poultry health by promoting energy regulation, mucosal integrity, immune homeostasis, and immune maturation. In this review article, we will focus on DFs, which directly interact with gut microbes and lead to the production of SCFAs. Further, we will discuss the current molecular mechanisms of how SCFAs are generated, transported, and modulated the pro-and anti-inflammatory immune responses against pathogens and host physiology and gut health. The maintenance of poultry gut health is complex depending on the intricate balance among diet, the commensal microbiota, and the mucosa, including the gut epithelium and the superimposing mucus layer. Changes in microflora composition and abundance can confer beneficial or detrimental effects on fowl. Antibiotics have devastating impacts on altering the landscape of gut microbiota, which further leads to antibiotic resistance or spread the pathogenic populations. By eliciting the landscape of gut microbiota, strategies should be made to break down the regulatory signals of pathogenic bacteria. The optional strategy of conferring dietary fibers (DFs) can be used to counterbalance the gut microbiota. DFs are the non-starch carbohydrates indigestible by host endogenous enzymes but can be fermented by symbiotic microbiota to produce shortchain fatty acids (SCFAs). This is one of the primary modes through which the gut microbiota interacts and communicate with the host. The majority of SCFAs are produced in the large intestine (particularly in the caecum), where they are taken up by the enterocytes or transported through portal vein circulation into the bloodstream. Recent shreds of evidence have elucidated that SCFAs affect the gut and modulate the tissues and organs either by activating G-protein-coupled receptors or affecting epigenetic modifications in the genome through inducing histone acetylase activities and inhibiting histone deacetylases. Thus, in this way, SCFAs vastly influence poultry health by promoting energy regulation, mucosal integrity, immune homeostasis, and immune maturation. In this review article, we will focus on DFs, which directly interact with gut microbes and lead to the production of SCFAs. Further, we will discuss the current molecular mechanisms of how SCFAs are generated, transported, and modulated the pro-and anti-inflammatory immune responses against pathogens and host physiology and gut health.
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