Nationalism and Ethnicity: Korean-Chinese Academics’ Views on China’s Multi-Ethnic Nationalism

Aihua Li 동북아역사재단 2016 Journal of Northeast Asian History Vol.13 No.1

Sparsity Adaptive Expectation Maximization Algorithm for Estimating Channels in MIMO Cooperation systems

( Aihua Zhang ),( Shouyi Yang ),( Jianjun Li ),( Chunlei Li ),( Zhoufeng Liu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.8

We investigate the channel state information (CSI) in multi-input multi-output (MIMO) cooperative networks that employ the amplify-and-forward transmission scheme. Least squares and expectation conditional maximization have been proposed in the system. However, neither of these two approaches takes advantage of channel sparsity, and they cause estimation performance loss. Unlike linear channel estimation methods, several compressed channel estimation methods are proposed in this study to exploit the sparsity of the MIMO cooperative channels based on the theory of compressed sensing. First, the channel estimation problem is formulated as a compressed sensing problem by using sparse decomposition theory. Second, the lower bound is derived for the estimation, and the MIMO relay channel is reconstructed via compressive sampling matching pursuit algorithms. Finally, based on this model, we propose a novel algorithm so called sparsity adaptive expectation maximization (SAEM) by using Kalman filter and expectation maximization algorithm so that it can exploit channel sparsity alternatively and also track the true support set of time-varying channel. Kalman filter is used to provide soft information of transmitted signals to the EM-based algorithm. Various numerical simulation results indicate that the proposed sparse channel estimation technique outperforms the previous estimation schemes.

Histone acetyltransferase inhibitors antagonize AMP-activated protein kinase in postmortem glycolysis

Qiong Li,Zhongwen Li,Aihua Lou,Zhenyu Wang,Dequan Zhang,Qingwu W. Shen 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: The purpose of this study was to investigate the influence of AMP-activated protein kinase (AMPK) activation on protein acetylation and glycolysis in postmortem muscle to better understand the mechanism by which AMPK regulates postmortem glycolysis and meat quality. Methods: A total of 32 mice were randomly assigned to four groups and intraperitoneally injected with 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR, a specific activator of AMPK), AICAR and histone acetyltransferase inhibitor II, or AICAR, Trichostatin A (TSA, an inhibitor of histone deacetylase I and II) and Nicotinamide (NAM, an inhibitor of the Sirt family deacetylases). After mice were euthanized, the Longissimus dorsi muscle was collected at 0 h, 45 min, and 24 h postmortem. AMPK activity, protein acetylation and glycolysis in postmortem muscle were measured. Results: Activation of AMPK by AICAR significantly increased glycolysis in postmortem muscle. At the same time, it increased the total acetylated proteins in muscle 45 min postmortem. Inhibition of protein acetylation by histone acetyltransferase inhibitors reduced AMPK activation induced increase in the total acetylated proteins and glycolytic rate in muscle early postmortem, while histone deacetylase inhibitors further promoted protein acetylation and glycolysis. Several bands of proteins were detected to be differentially acetylated in muscle with different glycolytic rates. Conclusion: Protein acetylation plays an important regulatory role in postmortem glycolysis. As AMPK mediates the effects of pre-slaughter stress on postmortem glycolysis, protein acetylation is likely a mechanism by which antemortem stress influenced postmortem metabolism and meat quality though the exact mechanism is to be elucidated.

Energy spread minimization in a cascaded laser wakefield accelerator via velocity bunching

Zhang, Zhijun,Li, Wentao,Liu, Jiansheng,Wang, Wentao,Yu, Changhai,Tian, Ye,Nakajima, Kazuhisa,Deng, Aihua,Qi, Rong,Wang, Cheng,Qin, Zhiyong,Fang, Ming,Liu, Jiaqi,Xia, Changquan,Li, Ruxin,Xu, Zhizhan American Institute of Physics 2016 Physics of plasmas Vol.23 No.5

Energy Efficiency Optimization for multiuser OFDM-based Cognitive Heterogeneous networks

( Bing Ning ),( Aihua Zhang ),( Wanming Hao ),( Jianjun Li ),( Shouyi Yang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.6

Reducing the interference to the licensed mobile users and obtaining the energy efficiency are key issues in cognitive heterogeneous networks. A corresponding rate loss constraint is proposed to be used for the sensing-based spectrum sharing (SBSS) model in cognitive heterogeneous networks in this paper. Resource allocation optimization strategy is designed for the maximum energy efficiency under the proposed interference constraint together with average transmission power constraint. An efficiency algorithm is studied to maximize energy efficiency due to the nonconvex optimal problem. Furthermore, the relationship between the proposed protection criterion and the conventional interference constraint strategy under imperfect sensing condition for the SBSS model is also investigated, and we found that the conventional interference threshold can be regarded as the upper bound of the maximum rate loss that the primary user could tolerate. Simulation results have shown the effectiveness of the proposed protection criterion overcome the conventional interference power constraint.

Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep

Liu, Shufang,Li, Hongbin,Song, Xuemei,Wang, Aihua,Wei, Caihong,Du, Lixin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.9

To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.

Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in nonheading Chinese cabbage

Guolin Zhou,Xia Li,Aihua Wang,Feng Zu,Zhenhua Hu,Jiazao Lin,Jinxing Tu 한국유전학회 2016 Genes & Genomics Vol.38 No.12

WS24-3 is a newly bred recessive genic male sterility line of the non-heading Chinese cabbage (Brassica rapa ssp. chinensis). Here, an F2 population was produced from the cross between WS24-3 and a male-fertile breeding line (WS135). The Illumina Brassica 60 K single nucleotide polymorphism (SNP) array was used for SNPs detecting between sterile and fertile bulks from the F2 population, and 62 SNPs were identified. BLAST analysis of the 62 SNPs revealed that the A2 chromosome of Brassica rapa genome contained 22 SNPs, whereas the other chromosomes did not contain more than 6 SNPs each. These data indicated that the potential target gene locus, named Bra2Ms, might be located on A2. Based on 10 of the 22 SNPs, allele-specific-polymerase chain reaction (AS-PCR) primers and single sequence repeat (SSR) primers were designed, 5 AS-PCR primers and 9 SSR primers showed difference between the bulks in electrophoretic determination. Analysis of these markers in F2 population revealed that Bra2Ms was genetically delimited to a region of 1.2 cM. We also detected two co-segregated markers SSRa2-951 and SSRa2-960 in this region. The markers identified in our study might facilitate the transfer of recessive genic male sterility alleles to other favorable genetic backgrounds. Furthermore, these markers will support a map-based clone of Bra2Ms.

Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non‑heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24‑3A

Liping Song,Xia Li,Feng Zu,Changbin Gao,Bincai Wang,Chufa Lin,Jinxing Tu,Aihua Wang,Guolin Zhou 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.

Classification and Identification of Bacteria in the Soil Treated by AcMNPV Using High-throughput Sequencing Technique

Yuejun Fu,Xing Li,Shuhua Zheng,Jun Du,Aihua Liang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) have been applied in the pesticide prevention as new bioinsecticides. Many studies have been carried out to assess the effects of insecticide on microorganism communities in different environments. However, little is known about whether the pesticides affect the microbial community in soil. Therefore, we performed high-throughput sequencing of V3–V4 hypervariable regions of the 16S rRNA genes from the bacteria in soil treated with AcMNPV and compared the difference of microbiota in these soil samples. In the study, a total of 80,301 validated reads were obtained, and the bacteria found belonged to 31 phyla and 748 genera. Statistical analysis showed that AcMNPV contributed the growth of Fusobacteria, Tenericutes, Cyanobacteria/Chloroplast, Actinobacteria, Gemmatimonadetes. AcMNPV inhibited the growth of Fibrobacteres, Crenarchaeota, Firmicutes, DeinococcusThermus, TM7, Chlorobi, Synergistetes, BRC1, Chlamydiae, Euryarchaeota, Planctomycetes, Acidobacteria, Verrucomicrobia, Bacteroidetes, Elusimicrobia, Nitrospira, Armatimonadetes, Proteobacteria, WS3, OD1, Chloroflexi, Spirochaetes. AcMNPV had no effect on SR1, OP11, Thermodesulfobacteria, Aquificae. Alpha Diversity analysis showed that the diversity of bacterial community for the soil treated with AcMNPV was lower than that of the soil before treatment or the control group. Meanwhile, the similarity of soil samples from AcMNPV treated group compared with samples from either untreated or prior treatment group was low as shown by Beta Diversity analysis. These findings provide previously unknown information about the impact of AcMNPV on the soil bacterial community structure and also lay a foundation for further investigations of AcMNPV how influences the development and progression of bioinsecticides.

Omp16, a conserved peptidoglycan-associated lipoprotein, is involved in Brucella virulence in vitro

Feijie Zhi,Dong Zhou,Junmei Li,Lulu Tian,Guangdong Zhang,Yaping Jin,Aihua Wang 한국미생물학회 2020 The journal of microbiology Vol.58 No.9

Brucella, the bacterial agent of common zoonotic brucellosis, primarily infects specific animal species. The Brucella outer membrane proteins (Omps) are particularly attractive for developing vaccine and improving diagnostic tests and are associated with the virulence of smooth Brucella strains. Omp16 is a homologue to peptidoglycan-associated lipoproteins (Pals), and an omp16 mutant has not been generated in any Brucella strain until now. Very little is known about the functions and pathogenic mechanisms of Omp16 in Brucella. Here, we confirmed that Omp16 has a conserved Pal domain and is highly conserved in Brucella. We attempted to delete omp16 in Brucella suis vaccine strain 2 (B. suis S2) without success, which shows that Omp16 is vital for Brucella survival. We acquired a B. suis S2 Omp16 mutant via conditional complementation. Omp16 deficiency impaired Brucella outer membrane integrity and activity in vitro. Moreover, inactivation of Omp16 decreased bacterial intracellular survival in macrophage RAW 264.7 cells. B. suis S2 and its derivatives induced marked expression of IL-1β, IL-6, and TNF-α mRNA in Raw 264.7 cells. Whereas inactivation of Omp16 in Brucella enhanced IL-1β and IL-6 expression in Raw 264.7 cells. Altogether, these findings show that the Brucella Omp16 mutant was obtained via conditional complementation and confirmed that Omp16 can maintain outer membrane integrity and be involved in bacterial virulence in Brucella in vitro and in vivo. These results will be important in uncovering the pathogenic mechanisms of Brucella.