Microbial conversion of major ginsenosides in ginseng total saponins by Platycodon grandiflorum endophytes

Cui, Lei,Wu, Song-quan,Zhao, Cheng-ai,Yin, Cheng-ri The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4

Background: In this study, we screened and identified an endophyte JG09 having strong biocatalytic activity for ginsenosides from Platycodon grandiflorum, converted ginseng total saponins and ginsenoside monomers, determined the source of minor ginsenosides and the transformation pathways, and calculated the maximum production of minor ginsenosides for the conversion of ginsenoside Rb1 to assess the transformation activity of endophyte JG09. Methods: The transformation of ginseng total saponins and ginsenoside monomers Rb1, Rb2, Rc, Rd, Rg1 into minor ginsenosides F2, C-K and Rh1 using endophyte JG09 isolated by an organizational separation method and Esculin-R2A agar assay, as well as the identification of transformed products via TLC and HPLC, were evaluated. Endophyte JG09 was identified through DNA sequencing and phylogenetic analysis. Results: A total of 32 ${\beta}$-glucosidase-producing endophytes were screened out among the isolated 69 endophytes from P. grandiflorum. An endophyte bacteria JG09 identified as Luteibacter sp. effectively converted protopanaxadiol-type ginsenosides Rb1, Rb2, Rc, Rd into minor ginsenosides F2 and C-K, and converted protopanaxatriol-type ginsenoside Rg1 into minor ginsenoside Rh1. The transformation pathways of major ginsenosides by endophyte JG09 were as follows: $Rb1{\rightarrow}Rd{\rightarrow}F2{\rightarrow}C-K$; $Rb2{\rightarrow}C-O{\rightarrow}C-Y{\rightarrow}C-K$; $Rc{\rightarrow}C-Mc1{\rightarrow}C-Mc{\rightarrow}C-K$; $Rg1{\rightarrow}Rh1$. The maximum production rate of ginsenosides F2 and C-K reached 94.53% and 66.34%, respectively. Conclusion: This is the first report about conversion of major ginsenosides into minor ginsenosides by fermentation with P. grandiflorum endophytes. The results of the study indicate endophyte JG09 would be a potential microbial source for obtaining minor ginsenosides.

Behavior Logic of Burmese Army Taking over Power in 2021——Based on Cost-Benefit Analysis

Cui Lei,Wang Miao 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.7 No.3

Since Myanmar started the process of transforming its political system, the country has progressively developed a political structure of dual power centers (Diarchy), with the NLD and the military taking turns holding the reins of power. Both sides are engaged in what might be described as a a military-political game, and fight without breaking. However, on February 1, 2021, when the new federal parliament of Myanmar conducted its first meeting, the Myanmar military decided to take over the rule, and the country entered a one-year state of emergency, known as the "February 1" Incident. As a result of this action by Myanmar’s military, a political environment that was stable has been disrupted. The causes are discussed in academic circles. There are objective reasons that the development and changes of political environment at home and abroad have greatly reduced the political space of the Burmese army, and there are subjective reasons that Min Onlai’s personal political ambition or his fear of war crimes trial after retirement should not be underestimated. This instance of political power seizure has been researched by scholars. there is no dearth of explanatory power, yet there are still some flaws. Therefore, this paper based on a cost-benefit analysis conducted from the viewpoint of the Burmese military, that the cost of taking over the regime is less than its benefit. Therefore, when the net income is positive, there is a high probability of taking over the regime.

Chemical Constituents from Rhizomes of Curculigo capitulata

Lei Di,Kai-Jin Wang,Cui-Cui Zhu,Ning Li 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.10

CHISELED NICKEL HYDROXIDE NANOPLATES GROWTH ON GRAPHENE SHEETS FOR LITHIUM ION BATTERIES

LEI-LEI TIAN,XIAN-YONG WEI,QUAN-CHAO ZHUANG,CHAO WU,RUI-LUN XIE,ZHI-MIN ZONG,YONG-LI CUI,SHI-GANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

The morphologies and structures of Ni(OH)2–graphene hybrid materials were tailored by using different mineralizers in this work. It was revealed that the synergic effects of the highly oxidized graphene sheets and the mineralizers played a crucial role in controlling the morphology and structure of the nanocomposites, and Na2CO3 is a very effective mineralizer for growing chiseled 2D nanoplates of Ni(OH)2 on graphene sheets. When produced with NaOH, fragmental Ni(OH)2 crystals with irregular shapes erratically decorated on graphene sheets. In contrast, chiseled Ni(OH)2 hexagonal nanoplates grown on graphene sheets were obtained when Na2CO3 was used as the mineralizer. These unique 2D–2D nanoarchitectures with higher contact area between the nanocrystals and graphene substrate can increase the interfacial interaction and then efficiently improve the structural stability of the composite material, thus exhibiting an enhanced Li storage capacity and excellent cycling performance of 562 mAh g-1 after the 36th cycle.

Overexpression of the AtSTK Gene Increases Salt, PEG and ABA Tolerance in Arabidopsis

Lei Bing,Cui-Cui Feng,Jing-Lan Li,Xiao-Xu Li,Baocun Zhao,Yin-Zhu Shen,Zhan-Jing Huang,Rong-Chao Ge 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6

AtSTK (At5g02800), which is a serine-threonineprotein kinase gene of Arabidopsis thaliana, was cloned, andits function was studied. The study found that the overexpressionof AtSTK could significantly improve the ability of A. thaliana to tolerate salt, PEG, and ABA stresses. RT-PCRanalysis revealed that the expression of the AtSTK genecould be obviously induced by salt, PEG, and ABA. Theexamination of the physiological characteristics showed thatthe overexpression of AtSTK in Arabidopsis significantlyreduced the plasma membrane permeability, significantlyincreased the proline content, and decreased the MDA content. These changes may reflect the physiological mechanismsthrough which AtSTK overexpression improves stress resistancein Arabidopsis. In addition, the overexpression of the AtSTKgene significantly antagonised the inhibitory effect of highconcentrations of exogenous ABA on Arabidopsis seedgermination. The subcellular localisation results showed thatAtSTK is located in both the cytosol and the nucleus. Theexamination of its tissue-specific expression showed thatAtSTK is expressed in various Arabidopsis tissues and isparticularly strongly expressed in the vessels. The signallingpathway analysis indicated that AtSTK might transfer thesalt stress signal in Arabidopsis through the MAPK pathway.

3-Dimensional Laboratory Experimental Validation of Sharp-Interface Optimization Model for the Control of Saltwater Intrusion

Lei Shi,Lei Cui,Sung-hun Hong,Namsik Park 대한토목학회 2006 대한토목학회 학술대회 Vol.2006 No.10

Salinity Estimation for a Coastal Pumping Well

Lei Shi(시뢰),Namsik Park(박남식),Lei Cui(최뢰),Kyungsoo Seo(서경수) 대한토목학회 2007 대한토목학회 학술대회 Vol.2007 No.10

A Secret Sharing Scheme Based on AES

Jie Cui,Lei Chen,Yiming Zhang,Zhiqiang Xie,Hong Zhong 보안공학연구지원센터 2014 International Journal of Security and Its Applicat Vol.8 No.6

In order to solve the key setting difficulty and the key security problem in the file encryption, key distributed storage technology may be a proper choice to help improve the safety of the key. In the paper, a novel secret sharing scheme is proposed by AES encryption algorithm for file confidentiality, dynamic key generation mechanism to generate keys, multi-secret-sharing ideas on key pre-treatment, using Shamir threshold scheme for secret dispersed storage. Finally, a few tests are carried out and the test results suggest that the efficiency of the whole scheme is good.

Chemical Constituents from Rhizomes of Curculigo capitulata

Di, Lei,Wang, Kai-Jin,Zhu, Cui-Cui,Li, Ning Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.10

단백질 종류 및 첨가수준이 In Vitro 발효특성과 반추위 미생물 성장에 미치는 효과

최뢰광(Cui, Lei Guang),송만강(Song, Man Kang) 충북대학교 동물생명과학연구소 2013 동물생명과학연구 Vol.5 No.-

본 실험은 서로 다른 단백질 원의 첨가 수준이 반추위액의 발효성상 및 미생물 체단백질 합성에 미치는 영향을 조사하기 위하여 연속배양 장치에서 일련의 in vitro 실험을 수행하였는 바, 가용성 단백질 중 peptone 또는 casein을 각각 0.5%와 1%씩 첨가할 경우 반추위 미생물에 의 한 발효와 미생물 생산량에 미치는 영향을 조사하였다. pH는 배양기간 동안 단백질원과 그 수준에 관계없이 낮아지는 경향이었고 대조구에 비하여 단백질원 첨가구에서 높았다(P<0.01). Ammonia-N의 농도는 단백질원을 첨가한 처리구들이 대조구에 비하여 유의적으로 높았으며 같은 단백질원 첨가구 중 첨가수준이 증가함에 따라 높았다(P<0.0001). 배양액중 total VFA 함량은 단백질원을 첨가한 처리구들의 경우 대조구에 비하여 높았으며, 첨가수준이 증가함에 따라 높게 나타났다(P<0.0001). Acetate의 농도는 배양시간이 경과함에 따라 감소하는 경향이었으나(P<0.05), propionate의 농도는 일정한 수준을 유지하였다(P>0.05). 따라서 C2/C3의 비율도 감소하였는데 peptone 1% 첨가구(2.68)에서 가장 낮았다(P>0.05). 이상의 결과를 종합해보면 단백질 분해로 생성된 암모니아에 비해 반추위 미생물 성장에 필요한 에너지 공급이 충분하지 못함으로서 단백질과 에너지의 동조화를 이루지 못하여 미생물 합성에 부정적인 영향을 미친 것으로 사료된다. In vitro continuous culture study was conducted to investigate the effect of protein source(peptone or casein) and it"s addition level(0.5% and 1%) to the culture solution on fermentation characteristics and rumen microbial growth. Ruminal pH was decreased with protein source. the control was lowest in pH among the treatments(P<0.0006). Ammonia concentration was higher in protein added treatments than in control, and its concentration was higher(P<.0001) for 1% added treatment than 0.5% added treatment. Control was lowest in total volatile fatty acid concentration in the culture solution rumen among treatments, and 1% addition was higher than 0.5% addition(P<.0001). Acetate(C2) proportion was decreased in all treatments as incubation time passed, and peptone addition at 1% level was lowest(P<0.05) among treatments. The C2/propionate ratio was decreased in all treatments as incubation time passed. There were no significant differences in ruminal microbial protein synthesis among protein sources and among addition levels. Results from this experiment indicate that protein source and its addition level did not significantly improve ruminal fermentation and microbial protein synthesis.