玉蜀黍 胚芽油의 變色現像 原因究明

李聖甲,成慶男 한경대학교 2004 論文集 Vol.26 No.1

Mixture of expressed & solvent extracted crude corn oil obtained from corn germ was carried out partial andlor total cooling process through heat exchange. Crude oil mixture at 40℃, 60℃ and 80℃ was storaged in storage tank under direct sunlight irradiation. Final deodorized(RBD) oil obtained through refining process used for storaged crude oil, it was determine color change of this RBD oil. RBD oils obtained were storaged in market and dark place as 0.9 ℓPET packaging bottle. Cause investigation of color reversion was attempted from Lovibond color change during storage period. At the result, color reversion wasn't appeared the linear correlation with refining process like to residual phosphorus con-tent, free fatty acids content and removal degree of pigments. As a result, cause of color reversion in corn oil was color fixation according to storage of crude oil at high temperature. Color fixed crude oil was not able to control at refining process. Prevention method of color reversion in corn oil was low temperature storage of crude oil at less than 40℃.

도태 젖소에서의 지방간 발생율에 관한 연구

이경갑 제주대학교 1993 논문집 Vol.37 No.-

. The liver of 203 slaughtered Holstein cows, were examined for their gross and histopathological observations. The fatty livers were classificated into mild(0 to 20%), moderate(20 to 40%) and severe(more than 40%). The results obtained were as follows; The number of cows with moderate and severs groups were 94(46.3%) and 43(21.2%), respectively. In the severe case with fatty changes, the size of the fatlet in the liver cell was larger than that of the mild ones. In the liver cells of the severe and moderate groups, the liver cell volume was increased, and the hepatic sinusoids were compressed by the liver cells. The nuclei were displaced to the periphery and lipogranulomas were present in the liver cells of the severe group.

Thioacetamide에 의한 BALB/c 마우스 간의 시간별 약물대사효소 억제 양상 : A Time-Course Study

이정운,고우석,김갑호,배연경,하현정,한상섭,천영진,정태천 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

Thioacetamide is a potent hepatotoxicant which requires metabolic activation by cytochrome P450s (P450s) for toxicity. In the present study, the elevation kinetic of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by thioacetamide treatment was investigated in male BALB/c mice. Inaddition, the inhibitory effects of thioacetamide on liver microsomal P450 enzymes were further investigated. Thioacetamide at 100 mg/kg/ was treated intraperitoneally for 6, 12, 24, 36, 48 and 72 hr. The blood was collected at the designated time for assaying the serum enzyme activities. To determine the P450 isozyme-specific activities. ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), and benzyloxyresorufin O-debenzylase (BROD) activities were determined for P450 1A1, 1A2 and 2B1, respectively, in liver microsomal fractions. The activities of ALT and AST were started to be elevated 6 hr after thioacetamide treatment andreached the maximun at 36 hr after the treatment. The elevated activities were dramatically recovered at 72 hr. The microscopic exmination of the liver specimen also showed a similar profile of hepatotoxicity. All P450-associated enzyme activities were time-dependently inhibited by the treatiment with thioacetamide. The maximum inhibition of P450 enzymes was observed 36 hr after the treatment. Because the inhibition of P450 enzymes by thioacetamide was time-dependent, our present results suggest that thioacetamide might inhibit P450 enzymes in mechanism-based inactivation.

Ethanolic Extract of Pancake Mixture Powder Supplemented with Helianthus tuberosus Enhances Antidiabetic Effects via Inhibiting Inflammatory Mediator NO Production

Lee, Kyoung-Dong,Sun, Hyeon-Jin,Lee, Mina,Chun, Jiyeon,Shin, Tai-Sun,Choi, Kap Seong,Shim, Sun-Yup The Korean Society for Microbiology and Biotechnol 2022 한국미생물·생명공학회지 Vol.50 No.2

Helianthus tuberosus is perennial plant as Compositae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixture powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro α-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3β activation in L6 cells. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory effects of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.

Biotinoyl domain of human acetyl-CoA carboxylase: Structural insights into the carboxyl transfer mechanism

Lee, Chung-Kyung,Cheong, Hae-Kap,Ryu, Kyoung-Seok,Lee, Jae Il,Lee, Weontae,Jeon, Young Ho,Cheong, Chaejoon Wiley Subscription Services, Inc., A Wiley Company 2008 Proteins Vol.72 No.2

<P>Acetyl-CoA carboxylase (ACC) catalyzes the first step in fatty acid biosynthesis: the synthesis of malonyl-CoA from acetyl-CoA. As essential regulators of fatty acid biosynthesis and metabolism, ACCs are regarded as therapeutic targets for the treatment of metabolic diseases such as obesity. In ACC, the biotinoyl domain performs a critical function by transferring an activated carboxyl group from the biotin carboxylase domain to the carboxyl transferase domain, followed by carboxyl transfer to malonyl-CoA. Despite the intensive research on this enzyme, only the bacterial and yeast ACC structures are currently available. To explore the mechanism of ACC holoenzyme function, we determined the structure of the biotinoyl domain of human ACC2 and analyzed its characteristics and interaction with the biotin ligase, BirA using NMR spectroscopy. The 3D structure of the hACC2 biotinoyl domain has a similar folding topology to the earlier determined domains from E. coli and P. shermanii. However, the local structures near the biotinylation sites have notable differences that include the geometry of the consensus “Met-Lys-Met” (MKM) motif and the absence of “thumb” structure in the hACC2 biotinoyl domain. Observations of the NMR signals upon the biotinylation indicate that the biotin group of hACC2 does not affect the structure of the biotinoyl domain, while the biotin group for E. coli ACC interacts directly with the thumb residues that are not present in the hACC2 structure. These results imply that, in the E. coli ACC reaction, the biotin moiety carrying the carboxyl group from BC to CT can pause at the thumb of the BCCP domain. The human biotinoyl domain, however, lacks the thumb structure and does not have additional noncovalent interactions with the biotin moiety; thus, the flexible motion of the biotinylated lysine residue must underlie the “swinging arm” motion. The chemical shift perturbation and the cross saturation experiments of the human ACC2 holo-biotinoyl upon the addition of the biotin ligase (BirA) showed the interaction surface near the MKM motif, the two glutamic acids (Glu 926, Glu 953), and the positively charged residues (several lysine and arginine residues). This study provides insight into the mechanism of ACC holoenzyme function and supports the swinging arm model in human ACCs. Proteins 2008. © 2008 Wiley-Liss, Inc.</P>

NMR elucidation of reduced B-Z transition activity of PKZ protein kinase at high NaCl concentration

Lee, Ae-Ree,Seo, Yeo-Jin,Choi, Seo-Ree,Ryu, Kyoung-Seok,Cheong, Hae-Kap,Lee, Shim Sung,Katahira, Masato,Park, Chin-Ju,Lee, Joon-Hwa Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>A Z-DNA binding protein (ZBP)-containing protein kinase (PKZ) in fish species has an important role in the innate immune response. Previous structural studies of the Zα domain of the PKZ from <I>Carassius auratus</I> (caZα<SUB>PKZ</SUB>) showed that the protein initially binds to B-DNA and induces B-Z transition of double stranded DNA in a salt concentration-dependent manner. However, the significantly reduced B-Z transition activity of caZα<SUB>PKZ</SUB> at high salt concentration was not fully understood. In this study, we present the binding affinity of the protein for B-DNA and Z-DNA and characterize its extremely low B-Z transition activity at 250 mM NaCl. Our results emphasize that the B-DNA-bound form of caZα<SUB>PKZ</SUB> can be used as molecular ruler to measure the degree of B-Z transition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The B-Z transition activity of caZα<SUB>PKZ</SUB> is uniquely dependent on salt concentration. </LI> <LI> caZα<SUB>PKZ</SUB> has reduced binding affinity for B-DNA at high NaCl. </LI> <LI> The equilibrium constant for the B-Z transition by a DNA-bound caZα<SUB>PKZ</SUB> was reduced. </LI> <LI> The chemical shifts of the B-DNA-bound form reflect the degree of B-Z transition. </LI> </UL> </P>

장기간 생존한 간전이를 동반한 부신피질 암종 1예

이현주,김명수,박효경,김대중,이유미,안철우,남재현,차봉수,송영득,한상원,임승길,김경래,이현철,허갑범 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

부신피질 암종은 매우 드문 질환으로 예후가 매우 나쁘다고 알려져 있으며 대부분의 경우에 큰 종괴와 stage Ⅳ의 진행된 상태로 진단된다. 가장 효과적인치료는 근치적 절제이며 수술적 절제만이 유일하게 치유할 수 있는 방법이며, 조기에 발견하여 완전절제를 하는 것이 장기생존율의 예후에 중요한 2가지 임상적 요인이다. Mitotane은 부신피질 암종에 사용되는 약제로 생존율을 증가시키는데 있어서 그 효과는 제한적이지만, 혈중농도를 감시하면서 적정 농도를 유지시킴으로서 생존율은 향상될 수 있다. 비록 근치적 절제가 되었다 하더라도 재발이나 전이가 매우 흔하며, 본 증례의 경우에 수술 후 16개월 만에 간전이가 발견되었으나 항암화학요법을 시행하면서 78개월간 생존하고 있는 간전이가 동반된 부신피질암종 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. An adrenal cortical carcinoma is a rare malignancy associated with poor prognosis. On diagnosis, most patients present with large tumor masses, which are often detected at an advanced stage. The most effective treatment is a complete resection, which is the only curative treatment for adrenal cortical carcinomas. The most important prognostic factor is a successful resection of the primary tumor, as long as it is low-grade and has not spread to distant sites. However, with advanced adrenal cortical carcinomas, with distant metastasis, there is no strict effective treatment program, and the prognosis is poor. The case of a 50-year-old female patient, presenting with an adrenal cortical carcinoma and Cushing's syndrome, who had a long-term survival of 78 months, is reported. The mass was completely resected on diagnosis, but 16 months later liver metastasis was discovered. She had received chemotherapy, with cisplatin, etoposide and bleomycin, for the liver metastasis for a period of 15 months, but with no response, furthermore, the size had increased after 10 months. Afterward, she received her 10th session of intrahepatic artery cisplatin chemotherapy and her 3rd hepatic artery embolization. Although the patient had a large degree of liver metastasis, this was tolerated. The tumor mass is presently not aggravated, and she still survives after 78 months (J Kor SOC Endocrinol 18:232-238, 2003).

Detection of Antibody to Candida albicans Proteinase in Sera from C. albicans-Cultured Patients and Healthy Controls

Kyoung-Ho Lee,Woon-Seob Shin,Hyun-Sook Park,Young Uh,In Ho Jang,Kap Jun Yoon,Choon-Myung Koh 大韓微生物學會 1997 大韓微生物學會誌 Vol.32 No.3

폐경후 여성에서 요중 안드로겐 대사물과 골밀도와의 상관 관계

이지현,허갑범,이현철,임승길,송영득,남수연,차봉수,원영준,김경래,권석호,정봉철 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.3

Background: Positive correlations between bone mass and androgen levels have been observed in premenopausal and postmenopausal women as well as in men. Androgen production was decreased in women with osteoporosis compared to that in age-matched controls. We hypothesized that androgen metabolism might be also deranged in osteoporosis. To clarify our hypothesis, we investigated the relationship between urinary metabolites of androgen and bone mineral density(BMD) in Korean postmenopausal osteoporotics. Methods: We examined the anthropometry and bone turnover marker in 67 postmenopausal women. BMD was measured by dual energy X-ray absorptiometry(DEXA). Serurn levels of estrone, estradiol, free testosterone were measured by radioirnmunoassay and serum level of sex hormone binding globulin(SHBG) was measured by two site immunoradiometric assay. The urinary metabolites of androgen were determined by gas chromatography-mass spectrometry (GC-MS) at Korean Institute of Science and Technology Doping Control Center. Results: 1. Spinal BMD had a positive correlation with height(r 0.3049, p$lt;0.05), weight (r=0.4114, p$lt;0.001) and body mass index (BMI, r=0.2638, p$lt;0,05). 2. Spinal and femoral neck BMD had no correlation with serum levels of estrone, estradiol and ten major urinary metabolites of androgen, but serum free testosterone had positive correlation with spinal BMD(r=0.3622, p$lt;0.01) and SHBG had negative correlation with femoral neck BMD (r= -0.2625, p$lt;(0.05). 3. Serum free testosterone in osteoporotics was lower than non-osteoporotics with spinal BMD(p$lt;0.05) and SHBG in patients with osteopenia was higher than non-osteopenic subjects with femoral neck BMD(p $lt;0.05). 4. In multiple stepwise regression analysis, weight and serum free testosterone were statistically significant for spinal BMD(R =0.3072). As for femoral neck BMD, weight was the independent determinant(R 0.1307). 5. Serum level of osteo#ealcin and urinary deoxypyridinoline/creatinine had a positive correlation with urinary 11-ketoandrosterone(p$lt;0.05). SHBG was positive correlation with osteocalcin (r=0.3190, p$lt;0.05). 6. Serum free testosterone (r= -0.2740, p$lt;0.05) decreased with aging. Conclusion: Our data suggest that androgen metabolism is not deranged in osteoporotics, but serum free testosterone is important than estrogen on postmenopausal osteoporosis after 5-10 years menopause. (J Kor Soc Endocrinol 12:450-462, 1997)

Preparation of carbon-containing, compressible, microporous, polymeric monoliths that regulate macroscopic conductivity

Lee, Kyoung Min,Kim, Hea Ji,Kang, Cheon-Soo,Tojo, Tomohiro,Chae, Ji Ae,Oh, Yuree,Cha, Min Chul,Yang, Kap Seung,Kim, Yoong Ahm,Kim, Hyungwoo The Royal Society of Chemistry 2019 Polymer chemistry Vol.10 No.7

<P>Porous polymer monoliths are of great importance as a multi-scale material in the fields of materials science and chemical engineering. Herein, we create compressible, microporous, composite monoliths that are capable of regulating macroscopic conductivity in response to external compressive force. The materials have been synthesized <I>via</I> a bottom-up approach and behave as sponge materials. The monolithic polymer networks could be prepared using various monomeric building blocks and further incorporated with carbon additives during condensation polymerization. Accordingly, physical properties, such as morphology, mechanical strength, and miscibility, were investigated. Taking advantage of microporosity of these frameworks, the carbon-containing composites were found to be fairly lightweight yet substantial, and non-conductive despite the presence of 10 wt% conductive carbon additives. However, the intrinsic compressibility of the networks significantly altered the resistivity of materials (<I>e.g.</I>, <I>ρ</I> < 10<SUP>2</SUP> Ω m) when the density of the materials increased following the application of an external force, which is comparable to doped silicon or other clays. As a proof of concept, we used the composite material as a switch for an electrical circuit. Therefore, we were able to repeatedly turn on and off a light-emitting diode in the circuit by hand.</P>