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結核病棟 入院患者 喀痰에서 分離한 Nocardia屬의 同定과 藥齋 減受性에 關한 實驗
高春明,金駿杰,李正碩,李一善,李沅泳 최신의학사 1974 最新醫學 Vol.17 No.9
This study was. carried out for the identification and drug susceptibility of Nocardia species iso?lated from the sputum of pulmonary tuberculosis patients at Severance Hospital from May, 1973 to May, 1974. The results as follows: 1. Among the 138 experimental specimens, eleven of the Nocardia species were identified by the methods of direct microscopic observation and culture characteristics. 2. Of the eleven Nocardia species, 6 of the Nocardia species were identified N. asteroides, 3 of the N. brasiliensis and 2 of the strains could not identified with a ordinary methods. 3. Antimicrobial activity of experimental strains to the several antibiotics, Ampicillin, Oxacilin and Cloxacillin were excellent against to the tested groups and Streptomycin, Chloramphenical and Gantrisin were also moderated effective to the experimental group.
土壤에서 分離한 Streptomyces의 抗菌性에 關한 硏究
高春明,柳駿 최신의학사 1975 最新醫學 Vol.18 No.9
This study was carried out for the identification of Streptomyces sp. isolated from some different geographical region soils and with evaluation of the active strains of antibiotic-producing Streptomyces sp. The results were summarized as follows: 1. Five hundred and seventy three strains of the Streptomyces strains isolated from 270 soil samples. 2. Of the isolated Streptomyces sp., 29.6 percent of the active strains were isolated. 3. These active Streptomyces strains showed the antibacterial activity against P. vulgaris ATCC 1272, K. pneumoniae KFCC 1268, S. aureus KFCC 1261 and S. typhi 42-A-63. 4. It is evident that the serial dilution method of antibiotic sensitivity, testing is the method of choice but disk method also available method for the screening of the antibiotic producing micro organisms.
우리나라에서 시판되고 있는 각종 가축사료에 대한 미생물학적 연구 : 유독성 진균의 검색 및 톡신 분석
고춘명 中央醫學社 1984 中央醫學 Vol.47 No.6
These studies were carried out to detect the presence of mycotoxin producing fungi and detoxification of toxins in various kinds of poultry feedstuffs in Korea. The experimental were divided into two parts: first the mycologic and second the toxicologic study. The results of experiment were summarized as follows: From the 54 various samples, 349 strains of fungi were isolated. In 336 of 349 strains, there were possible to identify in 8 genera. Among the identified strains, the predominant genera were Penicillium sp.(24.9%), Aspergillus sp.(22.9%) and Alternaria sp.(20.6%). In cytotoxicity test in Hela cells, 12 strains showed toxic effect among the 16 strains which were producing toxin-like substances. In the detoxification study with ultraviolet irradiation, all of the experimental toxic substances were denatured by the ultraviolet irradiation of 24 hours.
방선균 WCM-9가 생산하는 Polyene 항진균성 항생물질(AF1)의 생물학적 성상
신운섭,정선호,이동희,이경호,김수기,박주영,고춘명 대한의진균학회 1996 대한의진균학회지 Vol.1 No.1
Background: Pathogenic fungi infect humans, especially immunocompromised patients, with superficial or deeply invasive pattern. In the past 20 years, fungal infections have been increased dramatically resulted by increment of organ transplantation, cancer, AIDS patients, or use of broad-spectrum antibacterial agents. Fungal infections are now important causes of morbidity and mortality of hospitalized patients. but there is no effective antifungal antibiotics as well as antibacterial antibiotics Objective: Effective new antifungal antibiotics are needed for the treatment of mycosis. So in an effort to develop effective antifungal antibiotics, we screened over 600 isolates of Streptomyces spp. from soil. Methods: Antifungal producing strain was selected using disk diffusion method, An antifungal substance (AF1) was purified with ethyl acetate extraction, silica gel column chromatography and reverse phase HPLC. MICs of AF1 were detected by agar dilition method. Results: The compound showed UV maxima of 307, 321, 340, 359 nm indicating methylpentaene. Minimum inhibitory concentrations of the AF1 were 3.7 ㎍/ml against mold, and 3.7 - 7.4 ㎍/ml against Candida species. AF1 was also active against Cryptococcus neoformans, with MIC of 0.9 ㎍/ml. The concentration of AF1 for K^+ ion release from human red blood cell and hemolysis were 5 ㎍/ml. Conclusion: The antibiotic purified from culture broth of Streptomyces sp. WCM-9 was a polyene antifungal antibiotic which have broad spectrum antifungal activity.
抗生劑( Siccanin 및 Azalomycin-F )處理에 依한 Candida albicans의 電子顯微鏡的 觀察 : Siccanin and Azalomycin-F
高春明,崔泰周,등永健 최신의학사 1973 最新醫學 Vol.16 No.6
The present study is an ultrafine structure of Candida albicans treated with Siccanin and Azalomycin F by means of electron microscopy. The results as follows: 1. In contrast to the bacteria, the normal Candida albicans contains nuclear envelop, mitochondria, endoplasmic reticulum, distinct cell wall, cell membrane and nucleus as observed in the eucaryotic cells. 2. Treated Candida albicans with Siccanin and Azalomycin-F was changed their morphology and cytoplasmic components, for examples, thicking of cell wall, partial delation of nuclear envelop, mictochondria, detachment of cell membrane from cytoplasm and increasing of lipid particles. 3. When the cells were treated with the tested antibiotics at 10 mcg per ml. for 60 minutes, the cellular damges were most severe.
등영건,高春明,金聖光,孫瑀鍾,崔大卿 최신의학사 1974 最新醫學 Vol.17 No.4
The purpose of present study is to determine the toxicity of several mycotoxin-producing fungi, named A. flavus ATCC 15517, A. parasiticus RIB 1037, A. toxicarium RIB 4002, P. citrinum SWU 238, P. islandicum IFO 5235, P. tardum IFO 5787, and P. brunneum by observing the ultrastructural changes in the mouse liver cells. The results as follows: 1. All of the experimental animals treated with reference strains were observed the focal necrosis and inflammatory infiltration of liver parenchymal cell. 2. It showed cytoplamic changes such as dilatation of endoplasmic reticulum, swelling of mitochondria, detachment of ribosomes, increased number of lipid droplets and glycogens. 3. Nuclear and nucleolar alterations were also noted the nucleolar capping, segregation of nucleolar elements and irregularity of nuclear envelopes.