Detection of single nucleotide polymorphisms using a biosensor-containing titanium-well array.

Lee, Joo-Kyung,Cho, Si-Hyeong,Lee, Jinyoung,Lee, Jung-Hwan,Kim, Ah Young,Park, Bae Ho,Park, Jin-Goo,Busnaina, Ahmed,Lee, Hea Yeon American Scientific Publishers 2013 Journal of nanoscience and nanotechnology Vol.13 No.1

<P>The rapid identification and verification of single nucleotide polymorphisms (SNPs) were demonstrated using a well array sensor containing anti-biofouling titanium (Ti). Probe single-stranded DNA (ssDNA) was immobilized inside a titanium-well array on amine-modified glass surfaces with anti-biofouling behavior via a streptavidin-biotin interaction. Fluorescence intensity changes originating from the hybridization of nucleic acids to protein-bound nucleic acids linked to Alexa Fluor (FL) 647 were observed. The protocol was highly sensitive and reproducible for the detection of DNA hybridization. Significant changes in fluorescence signals were observed when using target DNA with a single base mismatch, indicating that this method is applicable to SNP detection. The microarray technology for the detection of SNPs using anti-biofouling Ti and other methods can be used as a highly sensitive in vitro medical sensor, as highlighted by an increase in genotyping accuracy.</P>

[PF-0032] Identification of the CaAN3 Gene that Regulates Fruit-specific Anthocyanin Biosynthesis in Pepper(Capsicum annuum)

Jinyoung Byun(Jinyoung Byun ),Tae-Gun Kim(Tae-Gun Kim ),Joung-Ho Lee(Joung-Ho Lee ),Ning Li(Ning Li ),Soyoung Jung(Soyoung Jung ),Byoung-Cheorl Kang(Byoung-Cheorl Kang ) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Association of Serum Biomarkers With Pulmonary Involvement of Rheumatoid Arthritis Interstitial Lung Disease: From KORAIL Cohort Baseline Data

( Jinyoung Moon ),( Jeong Seok Lee ),( Young Im Yoon ),( Sung Hae Chang ),( Yeon Ah Lee ),( You-jung Ha ),( Eun Ha Kang ),( Yong-beom Park ),( Hwa Jung Lee ),( Jung-yoon Choi ),( Eun Young Lee ) 대한류마티스학회 2021 대한류마티스학회지 Vol.28 No.4

Objective. The increase in mortality in rheumatoid arthritis (RA) patients with interstitial lung disease (ILD) is well known. However, there are few studies on serum markers that can evaluate acute exacerbation or prognosis in RA-ILD patients. The purpose of this study was to identify the association between biomarkers and lung lesions in patients with RA-ILD. Methods. We analyzed 153 patients with serum samples in a prospective, multicenter cohort of Korean RA-ILD patients. The serum levels of biomarkers, matrix metalloproteinase (MMP-7), surfactant protein-D (SP-D), and Krebs von den Lungen-6 (KL-6) were measured and correlated with forced vital capacity (FVC), diffusing capacity for carbon monoxide (DLCO) and the results of computed tomography (CT). CT results were interpreted semi-quantitatively according to the extent of lung lesions (grade 1, 0%∼25%; grade 2, 26%∼50%; grade 3, 51%∼75%; grade 4, 76%∼100%). Results. MMP-7, SP-D, and KL-6 were negatively correlated with FVC (MMP-7, r=-0.267, p=0.001; SP-D, r=-0.250, p=0.002; KL-6, r=-0.223, p=0.006) and DLCO (MMP-7, r=-0.404, p<0.001; SP-D, r=-0.286, p=0.001; KL-6, r=-0.226, p=0.007). In addition, MMP-7, SP-D, and KL-6 tended to increase with higher grades of lung lesions on CT (MMP-7, p=0.013; SP-D, p<0.001; KL-6, p<0.001). Conclusion. MMP-7, SP-D, and KL-6 can be used to evaluate the functional and anatomical status of lung involvement in the RA-ILD patients. (J Rheum Dis 2021;28:234-241)

Phospholipase-catalyzed hydrolysis in an artificial cell membrane in the presence of melittin.

Lee, Jinyoung,Lee, Joo-Kyung,Busnaina, Ahmed,Park, BaeHo,Lee, HeaYeon American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.1

<P>Biomimicry involves the use of the structure and function of biological systems as models for the design and engineering of materials and machines. An artificial cell membrane was developed using biomembrane components, and the membrane, formed by a lipid bilayer, was analyzed using surface plasmon resonance (SPR) to monitor hydrolysis by phospholipase (PL). The simultaneous atomic force microscope (AFM) images show that PL catalyzed the nanometer-scale hydrolysis of the artificial lipid biomembranes through enzymatic hydrolysis. In addition, it was confirmed that the combination of PL and melittin allowed the control of enzyme hydrolysis for the degradation of the lipid bilayer. Regarding the expected activating effect of melittin on hydrolysis, no difference with respect to the non-treated lipid membrane was observed in the AFM images. It is assumed that the partitioning of melittin into the membrane might prevent the binding or hydrolysis of Phospholipase A2 (PLA2). This study provides basic knowledge on a new approach for patterning biomimicking lipid membranes on a nano-scale.</P>

Combined Treatment with Demecolcine and 6-Dimethylaminopurine during Postactivation Improves Developmental Competence of Somatic Cell Nuclear Transfer Embryos in Pigs

Lee, Joohyeong,You, Jinyoung,Lee, Geun-Shik,Lee, Seung Tae,Hyun, Sang-Hwan,Lee, Eunsong Taylor Francis 2018 Animal biotechnology Vol.29 No.1

Tubercles patterns of the genus Linaeidea (Coleoptera: Chrysomelidae) from Korea

Jinyoung Park,HeeWook Cho,Jong Eun Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

The genus Linaeidea Motschulsky, 1860, contains six species, and is distributed in China, Japan, USSR, Europe, with only two species known in Korea (Gressitt and Kimoto, 1963; Seeno and Wilcox, 1982; Kimoto and Takizawa, 1994; Lee and An, 2001). Morphological notes of the immature stages and life history of Japanese L. aenea (Linne, 1758) were well studied by Kimoto (1962)and Kimoto and Takizawa (1994). Very little is known about the immature stages of this genus from Korea: only the larva of L. aenea have been briefly described and illustrated by Lee (1996). According to Hennig (1938), this genus is separable from the genus Chrysomela, in having sternal tubercles which are disappeared in the last instar larvae. However, the L. adamsi was not applied in the diagnosis character of this genus. The purpose of this results are to provide a key, detailed description, illustration and tubercles patterns of all known Korean species of genus Linaeidea as the basic data for the phylogenetic study the subfamily Chrysomelinae.

Acceleration of poly(L-lactide) degradation by TiO2 nanoparticles in sunlight.

Lee, Jinyoung,Lee, Ju-Kyoung,Park, Bae Ho,Busnaina, Ahmed,Lee, Hea Yeon American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.10

<P>Poly(L-lactide) (PLA) is known to eventually be degraded into water and carbon dioxide by the microorganisms of the natural world. Titanium dioxide (TiO2) has been used in the biomedical and bioengineering fields as a photocatalyst. The purpose of this research project is to evaluate the influence of TiO2 added to PLA films both before and after irradiation of the films with sunlight. The PLA-TiO2 films were prepared by the addition of TiO2 (size: > 100 nm) nanoparticles to PLA. The surface changes of the PLA film were investigated before and after the films were exposed to sunlight. Tiny holes were observed in the PLA film without TiO2 nanoparticles after irradiation with sunlight, whereas large pits formed in the PLA-TiO2 films. It is suggested that PLA was degraded by the ultraviolet rays in sunlight. In addition, the decomposition speed was thought to be enhanced by the addition of TiO2, which acted as a catalyst.</P>

Gold-layered calcium phosphate plasmonic resonants for localized photothermal treatment of human epithelial cancer

Kang, Jinyoung,Yang, Jaemoon,Lee, Jaewon,Oh, Seung Jae,Moon, Seyoung,Lee, Hong Jae,Lee, Sang Cheon,Son, Joo-Hiuk,Kim, Donghyun,Lee, Kwangyeol,Suh, Jin-Suck,Huh, Yong-Min,Haam, Seungjoo Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.19

<P>We fabricated <I>de novo</I> biocompatible mineral plasmon resonants for localized and systemic treatment of cancer. Biodegradable calcium-phosphate gold nanocomposites were synthesized for inducing a superior surface plasmon resonance effect. The combination of therapeutic antibody, Erbitux<SUP>®</SUP> and NIR laser with nanocomposites demonstrated the potential for selective bimodal cancer treatment by combination of EGFR-induced signaling inhibition as a systemic treatment and localized photothermal effects caused by the NIR laser.</P> <P>Graphic Abstract</P><P>De novo smart calcium-phosphate gold nanocomposites as superior surface plasmon resonants are developed for localized photothermal and systemic treatment of epithelial cancer. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b822835c'> </P>

섬망 환자의 치료에서 Haloperidol과 Quetiapine의 효과 비교 : 전향성 개방 연구

이진영(Jinyoung Lee),김상헌(Sang Heon Kim),서유진(Eugene Seo),이준영(Joon Young Lee),심제용(Jaeyong Shim),이양현(Yanghyun Lee) 대한생물치료정신의학회 2006 생물치료정신의학 Vol.12 No.2

본 연구는 섬망 환자를 대상으로 haloperidol 또는 quetiapine으로 치료하였을 때의 치료 효과와 부작용을 비교 평가하였는데, haloperidol군과 quetiapine군 모두에서 치료 후에 섬망 증상의 현저한 호전을 보였고, 환자가 섬망 증상에서 호전되는 데 걸린 기간에서도 차이를 보이지 않았다. 또한 사용한 두 약물을 등가용량으로 비교하였을 경우 quetiapine이 haloperidol 보다 적은 용량으로 섬망의 치료에 효과적이라는 것을 보였다. 대부분의 환자가 심각한 부작용이 없이 섬망에서 호전되었으나, haloperidol을 사용하였던 1명의 환자에서 추체외로계 부작용으로 인해 약물을 중단하였고, quetiapine을 사용하였던 환자에서는 추체외로계 부작용을 호소하는 환자가 없었다. Quetiapine을 사용하였던 2명의 환자에서 진정작용을 호소하였으나 경한 정도였고 약물을 중단하지는 않았다. Quetiapine은 섬망의 치료에서 haloperidol과 동등한 효과를 보이며, 특히 추체외로계 부작용이 적기 때문에 노인 환자의 섬망 치료에서 보다 안전하게 사용할 수 있으리라는 가능성을 보였다. 본 연구는 섬망이 발생한 환자들을 전향성으로 평가하였다는 데 의의가 있겠지만, 향후에 더 많은 수의 환자를 대상으로 한 이중맹검 위약대조군 연구가 필요할 것이다. Objectives:Delirium is a complex psychiatric syndrome with an acute onset and a fluctuating course. Typical antipsychotics, especially haloperidol, have been used as the main tool for pharmacological treatment of delirium. Recently, the newly developed atypical antipsychotics have been used successfully in control of delirium. The purpose of this prospective, open-clinical trial is to compare the clinical efficacy and tolerability of haloperidol and quetiapine for the treatment of delirium. Methods:Between July 2005 and June 2006, 36 patients who were diagnosed as having delirium according to the criteria of the DSM-IV were enrolled in this study from psychiatric practice settings and from consultation department. They were randomly assigned to be treated with a flexible-dose regimen of haloperidol(n=19) or quetiapine (n=17). K-DRS, K-DRS-R-98, CGI, MMSE-K, and ESRS had been assessed repeatedly until the K-DRS score reached 12 or less. We assumed that the patient whose K-DRS score had reached 12 or less was recovered from delirium. Results:Sixteen subjects in haloperidol group and sixteen subjects in quetiapine group completed the study. The mean dosage of antipsychotics for delirium is 2.9mg(SD=1.8) of haloperidol and 39.8mg(SD=28.9) of quetiapine. K-DRS and K-DRS-R-98 scores in both groups were significantly decreased after individual treatments. But there were no significant differences of changing extent of CGI-Improvement, MMSE-K, K-DRS and K-DRSR- 98 scores between the groups. There was no significant difference in duration of treatment and clinical improvement between the groups. Most of the patients were well tolerated to the adverse events of each antipsychotic drug but only one patient who had been treated with haloperidol showed extrapyramidal symptom after the medication. Conclusion:Quetiapine may be more useful than haloperidol in old aged patients with delirium because of its safety and low dose effectiveness. This trial is a prospective, open-label, flexible dose study with a small sample size, so further double-blind placebo-controlled studies with a large sample size will be necessary.

DHA 유도체를 이용한 항염, 항노화, 피부장벽 강화용 화장품 원료의 개발

이미영 ( Miyoung Lee ),이길용 ( Gil-yong Lee ),서진영 ( Jinyoung Suh ),이경민 ( Kyung Min Lee ),이우정 ( Woojung Lee ),조희원 ( Hee Won Cho ),이종재 ( Jong-jae Yi ),서정우 ( Jeong-woo Seo ),최헌식 ( Heonsik Choi ) 대한화장품학회 2021 대한화장품학회지 Vol.47 No.1

피부 염증은 흉터, 노화 뿐만 아니라 아토피와 같은 질환으로 발전할 수 있어 이를 조절하는 것이 매우 중요하다. 본 연구에서는 최근 인체에서 염증을 조절하는 것으로 알려진 specialized pro-resolving mediators (SPMs)의 in vitro 합성과 화장품 적용 가능성을 확인하였다. 대두의 lipoxygenase를 이용하여 mono 또는 di-hydroxy docosahexaenoic acid가 혼합된 시료 S-SPMs를 제작하였고 효능 평가에 이용하였다. 먼저, UVB로 염증을 유도한 세포에서 TNF-α와 IL-6의 발현이 S-SPMs에 의해 감소하고, 미세먼지에 의해 유도된 nitric oxide (NO)의 생성 역시 감소하는 것을 확인하여 S-SPMs의 항염 효능을 확인하였다. 또한, S-SPMs을 처리한 조건에서 malondialdehyde (MDA) 생성이 감소하여 지질 과산화 억제능이 있음을 확인하였고 S-SPMs에 의한 matrix metalloproteinase-1 (MMP-1)의 발현 감소, procollagen type I의 함량 증가를 통해 collagen 분해를 억제하고 반대로 합성은 촉진함을 확인하였다. 끝으로 filaggrin과 loricrin의 발현이 S-SPMs에 의해 증가한 것을 확인하여 피부 장벽 강화 효능을 확인하였다. 위 결과를 토대로 S-SPMs은 피부의 염증 억제와 함께 손상회복, 주름개선 및 장벽 강화를 위한 소재로 활용 가능함을 확인하였다. It is very important to control the inflammation of the skin because it can develop into diseases such as atopy as well as scarring and aging. In this work, we recently identified the in vitro synthesis of specialized pro-resolving mediators (SPMs) known to control inflammation in the human body and the applicability of cosmetics. Using recombinant protein of lipoxygenase from Glycine max, we succeeded to prepare mixtures of mono- or di-hydroxy DHA named as S-SPMs and used them for in vitro efficacy test. To investigate anti-inflammatory effect of S-SPMs, mRNA level of TNF-α and IL-6 were analyzed. Under UVB exposed condition, expression of both were decreased by S-SPMs treatment. And we observed reduced production of nitric oxide (NO) by S-SPMs application under the condition with diesel particulate matter (DPM). At the same experimental condition, malondialdehyde (MDA) production was decreased by S-SPMs, indicating the inhibitory effect of S-SPMs in lipid peroxidation. In addition, S-SPMs treatment resulted in reduction of matrix metalloproteinases-1 (MMP-1) expression and elevation of procollagen type I synthesis. Together with this, mRNA level of filaggrin and loricrin were increased by S-SPMs, indicating enhancement of skin barrier function. These results demonstrate that S-SPMs is a good candidate to develop as a cosmetic ingredient for anti-inflammation, anti-wrinkle, and improvement of skin barrier function.