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The putative role of carboxylesterase 2 in insecticide tolerance in Bemisia tabaci
Soyoung Kang,Deok-Ho Kwon,Jung Hun Oh,Si Hyeock Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The hemipteran whitefly Bemisia tabaci (Gennadius) is one of the most destructive pests damaging more than 600 agricultural crop species worldwide. The B and Q biotypes are most widely spread in Korea but they are not distinguishable based on morphological characters. Carboxylesterase 2 (Coe2) was determined to be 5.9 times more expressed in B biotype compared to Q biotype. Comparison of deduced amino acid sequences of Coe2 (595 a.a.) showed a total of 3.85% polymorphisms between B and Q types but no differences in major active sites. Quantitative real-time PCR revealed that both B and Q biotypes possess a single copy of coe2, suggesting that the overexpression of Coe2 in B biotype is likely due to overtranscription. To determine the putative role of Coe2 in insecticide tolerance, esterases were separated by native isoelectric focusing (IEF) and inhibited by various insecticides. The putative Coe2 band was apparently inhibited by pyrethroid and organophosphate insecticides, but not by imidacloprid. These findings suggest that overexpression of Coe2 confers chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration.
Development of protein and DNA markers for the identification of Bemisia tabaci biotypes B and Q
Soyoung Kang,Bum Joon Kim,Hye Jung Lee,Si Hyeock Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
The hemipteran whitefly Bemisia tabaci (Gennadius) is one of the most destructive pests damaging more than 600 agricultural crop species worldwide. The B and Q biotypes are most widely spread in Korea but they are not distinguishable based on morphological characters. In order to search for protein markers that can be employed for rapid and accurate diagnosis of biotypes, two-dimensional PAGE (2DE) in conjunction with mass spectroscopic analysis were conducted. Eleven biotype-specific spots were repeatedly identified during three repetitions of 2DE and analyzed by Q-TOF. One of the B type-specific protein spots was identified as carboxylesterase 2 (Coe2). The transcript level of coe2 was determined to be 6 times higher in B type than in Q type by quantitative real-time PCR. In addition, comparison of genomic DNA sequence of coe2 between B and Q types identified a biotype-specific intron, from which specific primer sets were designed. One-step PCR using these biotype-specific primers successfully distinguished the two biotypes in a high accuracy. Availability of the biotype-specific protein and DNA markers will greatly improve the detection of B. tabaci biotype in the field.
Soyoung Jung(정소영) 한국HCI학회 2012 한국HCI학회 학술대회 Vol.2012 No.1
Interface and layout design of smart devices' Operating System (OS) tend to be simpler, lighter and more intuitive than before. Compare with when the smartphones emerged and now, the interface and layout design of smartphones has certainly different styles. The evolvement has been towards to be simpler, lighter and more intuitive. The trends are that physical numbers of layers are getting bigger but each interface has less contents, the size of text for icon is getting smaller and the icon design also getting similar and looks very harmonious. Until now this trend, simpler, lighter and more intuitive is accelerating; the guidance for interface and layout design encourage this trend however, there are no empirical evidence. In this study will investigate the four trends in smart devices OS; first the number of layers in layout design, second the number of contents in each layers according to the screen size, third the text size according to icons size and screen size: it means more intuitive, lastly the icons are looks harmonious style.
Soyoung Jung,양우열,성인하 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.10
An observation on the relationship between stick-slip phenomena and chatter scratch generation during chemical-mechanical polishing (CMP) was made using finite element analysis. The motivation for this work is based on the questions about the possibility of the stickslip phenomena in the CMP process and the quantitative relation between the stick-slip behaviors and the formation characteristics of the scratch. The finite element simulation results showed that stick-slip friction could occur during CMP and it could be seen only in the case of the interactions with the particles trapped inside pad surface. Also, the stick-slip period increased with the density of interacting wafer surface material and the particle size had a significant effect on the stick-slip period and magnitude. It is expected that this work will aid the development of chatter scratch reduction technology.
Jung, Soyoung,Cho, Won,Lee, Hee Jung,Oh, Moonhyun WILEY-VCH Verlag 2009 Angewandte Chemie Vol.48 No.8
<P>This template will self-destruct: A unique particle-growth mechanism involves growth of new coordination polymers on the surface of initially formed hexagonal blocks and concomitant dissolution of the blocks to form hexagonal tubes (see scheme and scanning electron, optical, and fluorescence microscopy images). Calcination of the tubes yields ZnO particles of the same shape. <img src='wiley_img/14337851-2009-48-8-ANIE200804816-content.gif' alt='wiley_img/14337851-2009-48-8-ANIE200804816-content'> </P> <B>Graphic Abstract</B> <P>This template will self-destruct: A unique particle-growth mechanism involves growth of new coordination polymers on the surface of initially formed hexagonal blocks and concomitant dissolution of the blocks to form hexagonal tubes (see scheme and scanning electron, optical, and fluorescence microscopy images). Calcination of the tubes yields ZnO particles of the same shape. <img src='wiley_img/14337851-2009-48-8-ANIE200804816-content.gif' alt='wiley_img/14337851-2009-48-8-ANIE200804816-content'> </P>