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Phosphatidyl Inositol Metabolism and Initiation of DNA Synthesis
Lee, Myung Ae,Lee, Hyeong Ok,Jae, Cheol 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Intracellular inositol lipid hydrolysis involved in the initiation of DNA synthesis was examined. NIH 3T3 cells metabolically labeled with ³H-inositol were growth-arrested by serum starvation for 14 hours followed by 3mM hydroxyurea treatment for 6 hours and were allowed to initiate DNA synthesis in the normal media. The increased breakdown of phosphatidyl inositides into inositol 1,4,5-triphosphate and diacylglycerol was observed with the maximal DNA synthesis rate which was measured by the ³H-thymidine incorporation for 30 minutes during the progression of cell cycle into S phase. The incorporation of ^3H into phosphatidyl inositol-4-phosphate and inositol-l,4-bisphosphate in ³H-myo-inositol labeled cells was markedly increased with the initiation of DNA synthesis. These data suggest that increased phosphatidyl inositol turnover is associated with the initiation of DNA synthesis, and this process is in close relationship with the phosphorylation of phosphatidyl inositol into phosphatidyl inositol-4-phosphate by phosphatidyl inositol kinase, which is also phosphorylated into phosphatidyl inositol-4,5-bisphosphate by phospholipase C, which is in turn hydrolyzed to form inositol-1,4,5-triphosphate.
Lee, Jae-Won,Chun, Wanjoo,Kwon, Ok-Kyoung,Park, Hyun Ah,Lim, Yourim,Lee, Jae-Hyeon,Kim, Doo-Young,Kim, Jung Hee,Lee, Hyeong-Kyu,Ryu, Hyung Won,Oh, Sei-Ryang,Ahn, Kyung-Seop Elsevier 2018 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.64 No.-
<P><B>Abstract</B></P> <P>The increase in inflammatory cytokines and chemokines is a common denominator in the pathogenesis of acute lung injury (ALI) which are involved in the influx of inflammatory cells and lung damage. The aim of the present study was to evaluate the protective effect of 3,4,5-trihydroxycinnamic acid (THC) in lipopolysaccharide (LPS)-induced ALI. THC efficiently decreased the mRNA expression of interleukin-8 (IL-8) in LPS-stimulated A549 airway epithelial cells. THC induced heme oxygenase-1 (HO-1) expression in A549 cells. THC also increased the activation of AMP-activated protein kinase (AMPK) in A549 cells and RAW264.7 macrophages. In LPS-induced ALI in mice, THC significantly suppressed neutrophil influx and monocyte chemoattractant protein-1 (MCP-1) production in the bronchoalveolar lavage fluid (BALF). THC also attenuated the levels of neutrophil elastase (NE), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the BALF and serum. In addition, THC inhibited the expressions of inducible nitric oxide synthase (iNOS) and the activation of nuclear factor-kappa B (NF-κB) in the lung. These protective effects of THC were accompanied with HO-1 induction and AMPK activation. Taken together, the present study clearly demonstrates that THC significantly attenuates the LPS-induced ALI, suggesting that THC might be a valuable therapeutic adjuvant in airway inflammatory disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> THC reduces the mRNA expression of IL-8 in LPS-stimulated A549 airway epithelial cells. </LI> <LI> THC inhibits the influx of neutrophils and macrophages in the BALF of ALI mice. </LI> <LI> THC decreases the levels of NE, TNF-α and IL-6 in the BALF and serum. </LI> <LI> THC attenuates the recruitment of inflammatory cells and the production of MCP-1 in the lung. </LI> <LI> THC downregulates the activation of NF-κB and upregulates the activation of HO-1 and AMPK in the lung. </LI> </UL> </P>
Hyeong Ho Jo,Eun Young Kim,Jin Tae Jung,Joong Goo Kwon,Eun Soo Kim,Hyun Seok Lee,Yoo Jin Lee,Kyeong Ok Kim,Byung Ik Jang 대한소화기내시경학회 2022 Clinical Endoscopy Vol.55 No.2
Background/Aims: The diagnosis of intestinal tuberculosis (ITB) is often challenging. Therapeutic anti-tubercular trial (TATT) issometimes used for the diagnosis of ITB. We aimed to evaluate the changing pattern of fecal calprotectin (FC) levels during TATT inpatients with ITB. Methods: A retrospective review was performed on the data of 39 patients who underwent TATT between September 2015 andNovember 2018 in five university hospitals in Daegu, South Korea. The analysis was performed for 33 patients with serial FCmeasurement reports. Results: The mean age of the participants was 48.8 years. The final diagnosis of ITB was confirmed in 30 patients based on completemucosal healing on follow-up colonoscopy performed after 2 months of TATT. Before starting TATT, the mean FC level of the ITBpatients was 170.2 μg/g (range, 11.5-646.5). It dropped to 25.4 μg/g (range, 11.5-75.3) and then 23.3 μg/g (range, 11.5-172.2) afterone and two months of TATT, respectively. The difference in mean FC before and one month after TATT was statistically significant(p<0.001), and FC levels decreased to below 100 μg/g in all patients after one month of TATT. Conclusions: All ITB patients showed FC decline after only 1 month of TATT, and this finding correlated with complete mucosalhealing in the follow-up colonoscopy after 2 months of TATT.
Lee Seung-Su,Kim Hyoung-Geun,Park Eun-Ha,Kim Kwang Joong,Bang Myun-Ho,Kim Gayoung,Jeon Hyeong-Ju,Lee Chung-Gi,Shin Min-Chul,Kim Dae-Ok,Baek Nam-In 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.6
All parts of Thanakha (Hesperethusa crenulata R.) have been used as traditional skin care herbal material in Myanmar. In this study, coumarins from H. crenulata R. bark were isolated through solvent extraction, systematic solvent fractionation, and repeated column chromatography. Spectroscopic analyses using ESI–MS, 1D NMR (1H and 13C), 2D NMR (gHSQC and gHMBC), specific rotation, circular dichroism, and IR spectrometry revealed three coumarins 2R-7-hydroxy-8-(2,3-dihydroxy-3-methylbutyl)-coumarin (compound 1), peucedanol (compound 2), and methylpeucedanol (compound 3), which were first isolated from Thanakha tree. Antioxidant capacities of three coumarins decreased as follows: compound 2 > compound 3 > compound 1. Treatments of lipopolysaccharide-induced THP-1 human monocytic cells with compounds 2 and 3 at 378.8 μM and 359.7 μM inhibited tumor necrosis factor-α production by approximately 32.7% and 13.3%, respectively, compared with the negative control. In summary, these results suggest that Thanakha bark extracts can be used as a potent antioxidant and anti-inflammatory source for cosmetic ingredients.
Lee, Mee-Young,Yuk, Ji-Eun,Kwon, Ok-Kyung,Oh, Sei-Ryang,Lee, Hyeong-Kyu,Ahn, Kyung-Seop Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-
<P>We investigated whether Zuonin B exerts immunological effects on RAW264.7 cells. Zuonin B, isolated from flower buds of <I>Daphne genkwa</I>, suppressed the levels of nitric oxide and prostaglandin E<SUB>2</SUB>, as well as proinflammatory cytokines, such as tumor necrosis factor-<I><I>α</I></I> and interleukin-(IL-) 6, in lipopolysaccharide-stimulated macrophages. Moreover, the compound inhibited cyclooxygenase-2 and inducible nitric oxide synthase expression. Zuonin B attenuated NF-kappaB (NF-<I><I>κ</I></I>B) activation via suppressing proteolysis of inhibitor kappa B-alpha (I<I><I>κ</I></I>B-<I><I>α</I></I>) and p65 nuclear translocation as well as phosphorylation of extracellular signal-regulated kinase 1/2 and c-Jun N-terminal kinase. Additionally, IL-4 and IL-13 production in ConA-induced splenocytes was inhibited by Zuonin B. In conclusion, the anti-inflammatory effects of Zuonin B are attributable to the suppression of proinflammatory cytokines and mediators via blockage of NF-<I><I>κ</I></I>B and AP-1 activation. Based on these findings, we propose that Zuonin B is potentially an effective functional chemical candidate for the prevention of inflammatory diseases.</P>