Aflatoxin B_1 투여가 急性 肝 病變에 미치는 영향에 對한 病理學的 硏究

장선문,이충식,강대영 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In an attempt of elucidate the pathologic and histochemical changes of aflatoxin B_2-induced hepatie lesions, the present study in male Sprague-Dawley rats was undertaken to evalute the relation between pathologic change of the liver and histochemical changes of several enzymes : alkaline phosphatase, acid phosphatase and succinic dehydrogenase at 3. 9. 24. 48. 72, and 144 hours after single administration of aflatoxin B_1 by gastric tube. The aflatoxin B_1 was dissolved in peanut oil(0.5㎎ aflatoxin B_1 to 0.5ml peanut oil). Grouop of male rats received aflatoxin B_1 1㎎ per ㎏ of body weight, respectively. Control animals were given a similar volume of peanut oil alone. The results on morphologic and histochemical changes were as follows: 1. The necrotic change of liver was mainly periportal, and it became most severe at 24 hours and was more prominent in the large dose group than the small dose group. 2. Biliary ductular cell proliferation started to appear within 72 hours and after became regressed later in the small dose group, but it remained approximately 144 hours after exposure in the large dose group. 3. Electron microscopically, all three groups showed irregular nuclear membrane and nucleolar change characterized by segregation of granular and fibrillar elements. All the group showed cytoplasmie changes such as dilatation of rough endoplasmic reticulum with detached ribosome, hyperplasia of smooth endoplasmic reticulum, increased numbers of lipid droplets and mitochondrial alteration. The above change in the large dose group was more severe than those of the small dose group. 4. In the histochemical study of experimental animals, there was a decrease in the intensity of the staining reaction of alkaline phosphatase and succinic dehydrogenase within 24 hours and a progressive decrease in staining intensity was noted at 144 hours. And then the staining intensity of the acid phosphatase was similar to that shown by the control animal. In summary, the data obtained by present experiments indicate aflatoxin B_1 exerts periportal necrosis of hepatic lobules accompanied by bile ductular cell proliferation, and inhibition of protein synthesis, as jedged by elertron microscopic and histochemical changes.

Long second toe: its nail and skin changes

( Geun-hwi Park ),( Woo-il Kim ),( Min-young Yang ),( Won-ku Lee ),( Tae-wook Kim ),( Sung-min Park ),( Hyun-joo Lee ),( Gun-wook Kim ),( Hoon-soo Kim ),( Hyun-chang Ko ),( Byung-soo Kim ),( Moon-bum 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Nail can be affected by trauma, infection, and systematic disorders. In particular, the toe nails are exposed to the repeated and cumulative minor traumas, which usually result in thickened, splitted, and discolorated nails. Considering these, it can be inferred that the long second toe can be related to various nail and skin changes by repeated minor traumas such as friction in shoes, but the study or report on this topic hasn’t been done yet. Objectives: The purpose of this study was to introduce various nail and skin changes on long second toe. Methods: We analyzed the kinds of nail/skin changes of 50 patients with long second toe, enrolled at Pusan National University Hospitals (Busan and Yangsan) from 2013 to 2018. Results: There was no significant difference in the incidence of left and right toe and toenail, and 7 (14%) patients presented bilateral distribution. The most common nail change on long second toe was melanonychia (23, 46%), followed by subungual hematoma (15, 30%), onycholysis (2, 4%), subungual hyperkeratosis (2, 4%), onychomadesis (1, 2%), and retronychia (1, 2%). The most common skin change was corn (9, 18%), followed by paronychia (2, 4%). Twenty (20/50, 40%) patients also had other toe or toenail changes. Among them, the most common site was big toe/nail (13, 62%), and the most common change was subungual hematoma (8, 42%). Conclusion: Through this study, the dermatologists can recognize and diagnose the nail and skin changes of long second toe.

북서태평양에 서식하는 살오징어(Todarodes pacificus) 계군 분석에 대한 고찰

김정연,문창호,윤문근,강창근,김경렬,나태희,최은정,이충일,Kim, Jeong-Yun,Moon, Chang-Ho,Yoon, Moon-Geun,Kang, Chang-Keun,Kim, Kyung-Ryul,Na, Taehee,Choy, Eun Jung,Lee, Chung Il 한국해양학회 2012 바다 Vol.17 No.4

This paper reviews comparison analysis of current and latest application for stock identification methods of Todarodes pacificus, and the pros and cons of each method and consideration of how to compensate for each other. Todarodes pacificus which migrates wide areas in western North Pacific is important fishery resource ecologically and commercially. Todarodes pacificus is also considered as 'biological indicator' of ocean environmental changes. And changes in its short and long term catch and distribution area occur along with environmental changes. For example, while the catch of pollack, a cold water fish, has dramatically decreased until today after the climate regime shift in 1987/1988, the catch of Todarodes pacificus has been dramatically increased. Regarding the decrease in pollack catch, overfishing and climate changes were considered as the main causes, but there has been no definite reason until today. One of the reasons why there is no definite answer is related with no proper analysis about ecological and environmental aspects based on stock identification. Subpopulation is a group sharing the same gene pool through sexual reproduction process within limited boundaries having similar ecological characteristics. Each individual with same stock might be affected by different environment in temporal and spatial during the process of spawning, recruitment and then reproduction. Thereby, accurate stock analysis about the species can play an efficient alternative to comply with effective resource management and rapid changes. Four main stock analysis were applied to Todarodes pacificus: Morphologic Method, Ecological Method, Tagging Method, Genetic Method. Ecological method is studies for analysis of differences in spawning grounds by analysing the individual ecological change, distribution, migration status, parasitic state of parasite, kinds of parasite and parasite infection rate etc. Currently the method has been studying lively can identify the group in the similar environment. However It is difficult to know to identify the same genetic group in each other. Tagging Method is direct method. It can analyse cohort's migration, distribution and location of spawning, but it is very difficult to recapture tagged squids and hard to tag juveniles. Genetic method, which is for useful fishery resource stock analysis has provided the basic information regarding resource management study. Genetic method for stock analysis is determined according to markers' sensitivity and need to select high multiform of genetic markers. For stock identification, isozyme multiform has been used for genetic markers. Recently there is increase in use of makers with high range variability among DNA sequencing like mitochondria, microsatellite. Even the current morphologic method, tagging method and ecological method played important rolls through finding Todarodes pacificus' life cycle, migration route and changes in spawning grounds, it is still difficult to analyze the stock of Todarodes pacificus as those are distributed in difference seas. Lately, by taking advantages of each stock analysis method, more complicated method is being applied. If based on such analysis and genetic method for improvement are played, there will be much advance in management system for the resource fluctuation of Todarodes pacificus. 본 종설논문은 살오징어의 기존 및 최근에 새롭게 적용되고 있는 계군 분석방법들을 비교 분석하여 각 분석방법의 장단점과 분석방법간의 상호보완에 대하여 고찰하였다. 살오징어는 북서태평양의 넓은 지역을 회유하는 어종으로 생태계 및 상업적으로 중요한 자원이다. 살오징어는 해양환경변화의 생물학적 지표로서의 가능성을 평가 받고 있으며, 장단기적인 어획량 및 분포역의 변화가 환경 변화와 함께 나타난다. 예를 들어, 1987/1988 무렵에 발생한 기후체제전환 이후 한류성 어종으로 분류되는 명태의 어획량은 급감하여 현재까지 그 영향이 지속되고 있는 반면, 살 오징어 어획량은 크게 증가하였다. 현재까지 명태 어획량의 감소에 대하여 남획과 기후변화에 초점이 맞추어진 해석이 있으나, 뚜렷한 원인 분석은 이루어지지 않고 있다. 그 이유 중 한 가지는 계군 분석에 근거한 생태, 환경적 측면에 대한 정확한 원인 분석이 이루어지지 않고 있는 것과 관련이 된다. 계군은 유사한 생물학적 특징을 가진 개체들이 제한된 영역 내에서 유성생식과정을 통하여 동일한 유전자 풀(gene pool)을 공유하는 집단으로, 동일 계군을 형성하는 개체들은 산란에서 자원으로 가입 후 다시 재생산 과정에 이르기까지 시간 및 공간적으로 각기 다른 환경의 영향을 받을 수 있다. 따라서, 종에 대한 정확한 계군 분석은 자원의 효과적인 관리 및 급격한 변화에 대한 중요한 대응 방안의 역할을 할 수 있다. 살오징어 계군 분석에 적용된 주요 방법은 크게 4가지로 형태학적 방법, 생태학적 방법, 표지방류법, 유전학적 방법이 있다. 형태학적인 방법은 분석방법이 가장 간단하고 다수의 개체를 비교적 쉽게 분석할 수 있지만 각 형질들은 성장기간 동안 환경에 의해 영향을 많이 받게 되어 개체간의 차이가 생긴다. 생태학적 방법은 주로 개체의 생리적인 변화와 분포 및 회유상태, 기생충의 기생상태나 종류 및 기생률 등을 분석, 산란장의 차이를 알아보는 연구이며, 현재 활발히 연구되고 있는 방법으로 유사한 환경에서 생활하는 집단을 알 수 있지만 유전적으로 같은 집단인지는 알기 어렵다. 표지방류법은 직접적인 방법으로 계군의 회유 및 분포, 산란장의 위치를 파악할 수 있지만 수거가 어렵고 초기 단계에는 표식을 하기 어렵다. 수산생물의 계군 분석을 위한 유전학적 방법은 자원관리학적 연구에 관한 기본적 정보를 제공해 왔다. 계군 분석을 위한 유전학적 방법은 이에 사용하는 유전자 마커(marker)의 감도에 따라 결정되며, 유전자 마커의 다형성이 높은 것을 선택해야 한다. 계군 분석을 위한 유전자 마커로는 오랜 기간 동안 동위효소 다형이 사용되어졌으며, 최근에는 mitochondria, microsatellite와 같이 DNA 염기배열 중에서도 변이성이 높은 영역을 선택하여 마커로 이용한 연구가 증가되고 있다. 기존의 형태학적 방법, 표지방류법, 생태학적인 방법들은 살오징어의 생활사, 회유경로, 산란장의 변화 등을 밝혀내어 계군을 파악하는데 많은 기여를 하였지만 여전히 각 해역에 분포하는 살오징어의 계군을 파악하기에는 어려움이 있다. 최근에는 기존의 계군 분석이 지닌 장단점을 비교 분석하여 복합적인 방법의 계군 분석이 이루어지며, 이러한 정보들을 바탕으로 유전학적 방법을 보완한다면 살오징어 자원의 변동에 대한 관리 방안을 마련하는데 도움을 줄 것이다.

Effects of DK-002, a synthesized (6aS,cis)-9,10-Dimethoxy-7,11b-dihydro-indeno[2,1-c]chromene-3,6a-diol, on platelet activity

Lee, Ki-Seon,Khil, Lee-Yong,Chae, Sang-Ho,Kim, Deukjoon,Lee, Byung-Hoon,Hwang, Gwi-Seo,Moon, Chang-Hyun,Chang, Tong-Shin,Moon, Chang-Kiu 이화여자대학교 약학연구소 2008 藥學硏究論文集 Vol.- No.17

In the present study, the mechanism of antiplatelet activity of DK.-002, a synthesized (6aS,cis)-9.10-Dimethoxy-7,11b-dihydro-indeno|2,1-c ]chroniene-3.6a-diol, was investigated. DK-002 inhibited the thrombin, collagen, and ADP-induced rat platelet aggregation in a concentration- dependent manner, with 1C_(50) values of 120. 27, and 47 μM, respectively. DK-002 also inhibited thrombin-induced dense granule secretion, thromboxane A_(2) synthesis, and [Ca^(2+)], elevation in platelets. DK-002 did not show any significant effect on ADP-induced inhibition of cyclic AMP elevation by prostaglandin E_(1). but DK-002 was confirmed to inhibit ADP-induced [Ca^(2+)] elevation and shape change. DK-002 inhibited 4- bronw-A23187-induced [Ca^(2+)], elevation in the presence of creatine phosphate/creatine phosphokinase (CP/CPK, a ADP scavenging system) and indomethacin (a specific inhibitor of cyclooxygenase). DK-002 also inhibited Ca^(2+) mobilization in thrombin- or 4-bromo-A23187-stimulated platelets through its inhibitory effects on both Ca^(2+) release from intracellular stores and Ca^(2+) influx, in the presence of CP/CPK and indomethacin. Taken together, the present study shows that DK-002 has inhibitory effects on stimulation of platelets, and suggests that its antiplatelet activity might be related to the inhibitory mechanism on Ca^(2+) mobilization in stimulated platelets. ⓒ 2005 Elsevier Inc. All rights reserved.

개에서 동소성 간이식술시의 대사 및 혈역학적 변화

이창준,홍순용,신근만,최영룡,이영주,문종선 대한마취과학회 1993 Korean Journal of Anesthesiology Vol.26 No.3

Skilled and experienced anestheia is of great importance for patients undergoing orthotopic liver transplantation, because of multiple preexisting medical problems in such patients as well as the intraoperative problems of rapid hemodynamic, metabolic, and coagulation changes. In this study, the intraoperative hemodynamic and laboratory data were analyzed in ten dogs that underwent an orthotopic liver transplantation procedure by veno-venous bypass using Biopump. Liver transplantation can be divided into three distinct periods: stage I, or preanhepatic stage, which begins with the induction of anesthesia and continues until cross clamping of portal vein and IVC; stage II, or anhepatic stage, which begins at the anhepatic time and continues until the donor liver is reperfused by the recipients circulating blood; and stage III, or postanhepatic stage, which begins at the time of reperfusion and continues until the end of surgical procedure. The hemodynamic changes at the time of IVC and portal vein cross clamping were decreases in CVP, PCWP, and pulmonary artery pressure in spite of using Biopump. The significant metabolic alternations during anhepatic stage were decrease in blood glucose levels and increase in blood lactate levels. The more significant hemodynamic changes occurred at the time of reperfusion. Systolic pressure decreased suddenly to 58±6 mmHg and cardiac output decreased to 1.08±0.1l L/min. However heart rate, pulmonary artery pressure, CVP, and PCWP did not change significantly. During stage III, hyperglycemia occurred quite frequently. Significant abnormal coagulation chages could not be found, probably because the dogs were healthy. In conclusion, during anhepatic stage, we have to compensate for alternations of fluid balance. At the time of reperfusion, we should prevent severe hemodynamic changes and treat them immediately if they occur. However, it seems that glucose administration is not necessary to the liver recipient during stage II because there is no significant hemodynamic depression e to hypoglycemia at this time and hyperglycemia occurs later

4분과 : 미래 기후변화 시나리오에 따른 우리나라 소나무 임부의 재적 추정

김문일 ( Moon Il Kim ),이우균 ( Woo Kyun Lee ),( Gui Shan Gui ),( Hang Nan Yu ),최솔이 ( Sol E Choi ),김창길 ( Chang Gil Kim ),권태성 한국임학회 2014 한국산림과학회지 Vol.103 No.1

본 연구는 우리나라 주요 수종인 소나무림을 대상으로 RCP(Representative Concentration Pathway)8.5 시나리오에 따른 임목 재적의 시·공간적 변이를 예측하기 위해 수행되었다. 전국 규모의 예측을 위해 5차임상도와 국가산림자원조사 자료를 이용하였으며, 기후와 공간의 변이가 임목 생장에 미치는 영향을 반영하기 위해 기상 및 지형인자를 반영한 생장모형을 적용하였다. 모형의 검증을 위해 시, 도별 산림통계와 모형 결과를 비교한 결과, 비교적 높은 적합도를 보이는 것으로 나타났다. 기후변화를 고려하였을 때, 소나무림의 임분 재적은 현재 131 m3/ha에서2050년에는 212.42 m3/ha까지 증가 할 것으로 예측되었으며, 현재의 기후가 유지될 경우에는 221.92 m3/ha까지 증가할 것으로 예측되었다. 기후변화의 영향으로 인해 일부 고산지대를 제외한 대부분의 지역에서 소나무림의 생장률이감소할 것으로 예측되었으며, 특히 해안지역과 남부지역에서 생장률의 감소가 클 것으로 나타났다. 본 연구결과를 통해 기후변화가 소나무림 생장에 미치는 영향을 시·공간에 따라 정량화 할 수 있었으며, 이는 기후변화 적응을 고려한 산림관리 및 시업계획을 수립하는데 유용하게 활용될 수 있을 것이다. The main purpose of this study is to measure spatio-temporal variation of forest tree volume basedon the RCP(Representative Concentration Pathway) 8.5 scenario, targeting on Pinus densiflora forests which is the main tree species in South Korea. To estimate nationwide scale, 5th forest type map and National Forest Inventory data were used. Also, to reflect the impact of change in place and climate on growth of forest trees, growth model reflecting the climate and topography features were applied. The result of the model validation, which compared the result of the model with the forest statistics of different cities and provinces, showed a high suitability. Considering the continuous climate change, volume of Pinus densiflora forest is predicted to increase from 131 m3/ha at present to 212.42 m3/ha in the year of 2050. If the climate maintains as the present, volumeis predicted to increase to 221.92 m3/ha. With the climate change, it is predicted that most of the region, except for some of the alpine region, will have a decrease in growth rate of Pinus densiflora forest. The growth rate of Pinus densiflora forest will have a greater decline, especially in the coastal area and the southern area. With the result of this study, it will be possible to quantify the effect of climate change on the growth of Pinusdensiflora forest according to spatio-temporal is possible. The result of the study can be useful in establishing the forest management practices, considering the adaptation of climate change.

Sarcomatoid Carcinoma Arising from Mature Cystic Teratoma

Ho-Chang Lee1, Seung-Myoung Son, Yong-Moon Lee, Ji Hae Koo, Song-Yi Choi, Ok-Jun Lee, Eun-Hwan Jeong 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

Malignant transformation of mature cystic teratoma (MCT) is rare. Sarcomatoid carcinoma is a neoplasm comprising malignant mesenchymal cells and a conventional carcinomatous area. Here, we report on a case of sarcomatoid carcinoma arising from an MCT in the left ovary of a 45-year-old female. A unilocular cyst consistent with MCT was observed; however, a nodule within the cyst was confirmed from the resected ovary. Microscopically, the nodule showed both squamous cell carcinoma and pleomorphic sarcomatous components admixing with each other. Lining epithelial cells at the periphery of the main tumor showed squamous metaplasia. When a sarcomatous component is observed in the ovary tumor, it is important to find a squamous cell component, either benign or malignant.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Association Between Gut Regulatory Hormones and Post-operative Weight Loss Following Gastrectomy in Patients With Gastric Cancer

Hye-Kyung Jung,Chung Hyun Tae,Hye Ah Lee,Ko Eun Lee,Chang Mo Moon,Seong Eun Kim,Ju Young Seoh,Joo-Ho Lee 대한소화기 기능성질환·운동학회 2022 Journal of Neurogastroenterology and Motility (JNM Vol.28 No.3

Background/AimsPost-operative weight loss in patients with gastric cancer lead to a poor quality of life and long-term survival. This study aims to evaluate the effects of gut regulatory hormones on post-operative weight loss in patients with subtotal gastrectomy for gastric cancer. MethodsThis prospective study was conducted for 12 months post-surgery in 14 controls and 13 gastrectomy patients who underwent subtotal gastrectomy for gastric cancer. Serum plasma ghrelin, glucagon-like peptide-1, gastric inhibitory peptide-1, peptide YY, insulin, and homeostatic model assessment for insulin resistance responses to a standardized test meal were recorded at multiple time points before and after gastrectomy at 4 and 12 months. ResultsThe mean weight difference between the pre-operative state and the 4-month period was significantly reduced to 6.6 kg (P = 0.032), but significant weight reduction was not observed from 4 months to 12 months. The plasma levels of glucagon-like peptide-1, gastric inhibitory peptide-1, and peptide YY were significantly increased 4 months postoperatively compared to the pre-operative state (all P = 0.035); however, pre-operative levels and relative changes over a period of 0-4 months of hormones were not correlated with body weight changes. Only the pre-operative ghrelin at peak had a negative correlation with changes in weight reduction in the 4 months after surgery (ρ = −0.8, P = 0.024). ConclusionsSignificant weight reduction was common after subtotal gastrectomy for gastric cancer with a negative correlation pre-operative plasma ghrelin levels. Incretin hormones are modestly but significantly increased after subtotal gastrectomy; however, these changes did not affect the weight changes.

Design of Digital Filter to minimize An Afterimage Phenomenon occurring in processing Image Signal

Moon, O Hak,Lee, Bong Geun,Lee, Ho Nam,Lee, Young Ho,Kang, Bong Soon,Hong, Chang Hee 동아대학교 정보기술연구소 2001 情報通信硏究所論文誌 Vol.8 No.2

In this paper, we propose a method, an optimized architecture of a device with an image signal process of a field unit to minimize the flick phenomenon that happens in direction of a color temperature at a color tone change. The proposed IIR loop filter has an optimized architecture and reduced hardware compared with previous filters. In order to achieve the optimization for the hardware complexity. It is designed by time-multiplexing architecture. The proposed IIR loop filter is synthesized by suing the STD90 0.35um cell library.