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Pharmacogenomic landscape of patient-derived tumor cells informs precision oncology therapy
Lee, Jin-Ku,Liu, Zhaoqi,Sa, Jason K.,Shin, Sang,Wang, Jiguang,Bordyuh, Mykola,Cho, Hee Jin,Elliott, Oliver,Chu, Timothy,Choi, Seung Won,Rosenbloom, Daniel I. S.,Lee, In-Hee,Shin, Yong Jae,Kang, Hyun J Nature Pub. Co 2018 Nature genetics Vol.50 No.10
Ku, Bonsu,Yun, Hye-Yeoung,Lee, Kyung Won,Shin, Ho-Chul,Lee, Sang-Rae,Kim, Chang Hyen,Park, Hwangseo,Yi, Kyu Yang,Lee, Chang Hoon,Kim, Seung Jun Elsevier 2018 Bioorganic & medicinal chemistry Vol.26 No.18
<P><B>Abstract</B></P> <P>Cytosolic protein tyrosine phosphatase epsilon (cyt-PTPε) plays a central role in controlling differentiation and function of osteoclasts, whose overactivation causes osteoporosis. Based on our previous study reporting a number of cyt-PTPε inhibitory chemical compounds, we carried out a further and extended analysis of our compounds to examine their effects on cyt-PTPε-mediated dephosphorylation and on osteoclast organization and differentiation. Among five compounds showing target selectivity to cyt-PTPε over three other phosphatases <I>in vitro</I>, two compounds exhibited an inhibitory effect against the dephosphorylation of cellular Src protein, the cyt-PTPε substrate. Moreover, these two compounds caused destabilization of the podosome structure that is necessary for the bone-resorbing activity of osteoclasts, and also attenuated cellular differentiation of monocytes into osteoclasts, without affecting cell viability. Therefore, these findings not only verified anti-osteoclastic effects of our cyt-PTPε inhibitory compounds, but also showed that cyt-PTPε expressed in osteoclasts could be a putative therapeutic target worth considering.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Ionic Conductivity of Anion Receptor Grafted Siloxane Polymers for Solid Polymer Electrolytes
Lee, Won-Sil,Kim, Dong-Wook,Lee, Chang-In,Woo, Seong-Ihl,Kang, Yong-Ku The Korean Electrochemical Society 2011 Journal of electrochemical science and technology Vol.2 No.1
We have prepared siloxane polymers grafted with trifluoromethane-sulfonylamide and oligoether side chains for solid polymer electrolytes with enhanced ionic conductivity. The grafted trifluoromethane sulfonylamide groups seem to be effective as an anion recepting site to enhance the ionic conductivity of the solid polymer electrolyte. The anion receptor grafted siloxane polymers showed one order of magnitude higher ionic conductivity than the siloxane polymers without anion receptor grafts. The fitting parameter A of the VTF plot which was related to the carrier density of the electrolyte increased with increasing the number of grafted anion receptor. The results of experiment indicate that the anion-complexing site of the anion receptor grafted polymer host effectively traps the anions. The anion receptor grafted polymer was found to be a promising material for lithium polymer batteries.
Increased productivity of the baculovirus expression vector system by combining enhancing factors
Lee, Ji-Hoon,Gwak, Won-Seok,Bae, Sung-Min,Choi, Jae-Bang,Han, Beom-Ku,Woo, Soo-Dong 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol. No.
<P><B>Abstract</B></P> <P>The baculovirus expression vector system uses the polyhedrin promoter for the expression of foreign proteins. To increase the polyhedrin promoter activity, vectors were constructed by combining two transcription enhancers and the vp39 promoter. The transcription enhancers included homologous region 5 (hr5) and burst sequences (BSs). Seven vectors were constructed using combinations of these transcription enhancers, and their expression levels were compared in <I>Autographa californica</I> multicapsid nuclepolyhedrovirus (AcMNPV) and <I>Bombyx mori</I> NPV (BmNPV) using enhanced green fluorescent protein (EGFP). Our results indicate that most of the constructs increased the expression level of EGFP using the polyhedrin promoter. In particular, the vp39 promoter and BSs were the most effective in BmNPV and AcMNPV, respectively. Synergistic effects of the enhancing factors were observed only in AcMNPV by the vp39 promoter and BSs. The hr5 negatively affected the expression level of EGFP using the polyhedrin promoter and other enhancers in both viruses. The enhancing effect obtained by combining the three factors was higher in BmNPV than in AcMNPV. This was the first study to perform a comparative evaluation of enhancing factors that affect the expression efficiency of foreign protein using the polyhedrin promoter in both AcMNPV and BmNPV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Burst sequence (BS) repeats and vp39 promoter were most effective in BEVS. </LI> <LI> The hr5 negatively affected the expression levels of the polyhedrin promoter. </LI> <LI> Synergistic effect was observed only in AcMNPV by the vp39 promoter and BSs. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
SiC Single Crystal Growth on Dual Seed with Different Surface Properties
Lee, Sang Il,Jung, Jung Young,Park, Mi Seon,Lee, Hee Tae,Lee, Doe Hyung,Lee, Won Jae,Hong, Soon Ku,Yeo, Im Gyu,Kim, Heung Rak,Chun, Myong Chuel Trans Tech Publications, Ltd. 2013 Materials science forum Vol.740 No.-
<P>SiC crystal ingots were grown on 6H-SiC dual-seed crystal with different surface properties by a PVT (Physical Vapor Transport) technique. And then SiC crystal wafers sliced from the SiC ingots were systematically investigated in order to find out the dependence of surface properties for seed on the polytype formation. While n-type SiC crystals exhibiting the 4H polytype were grown on seed crystal having high root-mean-square (rms) value, 6H-SiC crystals were grown on seed having lower rms value. However, 6H polytype was maintained on on-axis and off-axis seeds during the entire growth period. The crystal quality of 6H-SiC single crystals grown on on-axis seed were revealed to be slightly better than that of 6H-SiC crystal grown on off-axis seed.</P>
Role of Glutathione Conjugation in 1-Bromobutane-induced Immunotoxicity in Mice
Lee, Sang-Kyu,Lee, Dong-Ju,Jeon, Tae-Won,Ko, Gyu-Sub,Yoo, Se-Hyun,Ha, Hyun-Woo,Kang, Mi-Jeong,Kang, Won-Ku,Kim, Sang-Kyum,Jeong, Tae-Cheon Korean Society of ToxicologyKorea Environmental Mu 2010 Toxicological Research Vol.27 No.2
Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 mg/kg in corn oil once for dose response or treated orally with 1-BB at 1500 mg/kg for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.