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정경권,정성부,엄기환 동국대학교 산업기술환경대학원 1998 산업기술논총 Vol.4 No.-
In this paper, we propose a nonlinear system identification method using genetic algorithm. We represent the nonlinear system as a parameter vector and a measurement vector. In order to identify the nonlinear system, we find the parameter vector using genetic algorithm. The parameter vector is regarded as a chromosome of gene. The error between the desired output and estimated output every sampling period is used to calculate the fitness of one gene. The simulation results showed the effectiveness of using the genetic algorithm in the nonlinear system identification.
온주밀감(Citrus unshiu Marc. var. Okitsu) 미분화 종바로부터 callus 유기 및 증식
진성범,홍경애,부경환,이도승,류기중 제주대학교 방사능이용연구소 2000 연구보고 Vol.14 No.-
조직배양을 통한 기관분화나 식물체 재분화 가 어려운 것으로 알려져 있는 온주밀감(Citrus unshiu Marc. var.Okitsu) 미분화 종자로부터 callus 유기 조건을 확립하였다. 온주밀감 성숙과실의 미분화 종자를 MT(Murashige and Tucker, 1969) 기본배지에 치상 6주 후에 미숙종자 양쪽 가장자리에 유기된 조그만white callus를 gibberellin 1mg/L, adenine 25mg/L, malt extract 500mg/L가 첨가된 MT 기본배지에 치상 하여 2주간격으로 계대배양하여 캘러스를 유지할 수 있었고, EME(Grosser and Gmitter, 1990a ) 배지에 4주 간격으로 계대배양함으로써 증식시킬 수 있었다.
사망진단서(사체검안서) 상의 선행사인으로부터 사망통계의 원사인이 선정되는 비율 : 3개 대학병원에서 교부된 사망진단서를 중심으로
박우성,박석건,정철원,김우철,탁우택,김부연,서순원,김광환,서진숙,부유경 한국의료QA학회 2004 한국의료질향상학회지 Vol.11 No.1
Background : To exatnine the problems intolved in writing practice of death certificates, we cotnpated the determination of underlying cause of death for wital statistics using recorded underlying cause of death in issued death statistics. Methods : We collected 688 rnortality certificates issue in year of 2,000 from 3 university hospitals. And we also collected vital statistics from ministry of statistics. The causes of death were coded by experienced medical record wpecialists. And causes of death determined at ministry of statistics for national vita statistics were mapped to causes of death recorded at each death certificates. The rate that underlying causes of death for vital statistics were derived from underlying causes of death recorded at issued death certificaties sere analysed. Results : 64.5% of underlying cause of death for could be derived from underlying cause of death recorded at issued death certificates, 8.6% derived from intermediate cause of death, and 3.9% derived from direct cause of death. In 23% of cases, underlying cause of death could not be derived using issued death certificates. The rate that underlying cause of death for vital statistics could be derived from underlying cause of death recorded at death certificates was different between 3 university hospitals. Ane the rate was also different between death certificates and postmortem certificates. We classified the causes of death using 21 major categories. The rate was different between diseases or conditions tha caused death too. Conclusion : When we examined the correctness of death certificate writing practice using abpve methods, cortectness of writing could not be told as satisfactory. There was difference in correctness of writing between hospotals, between death certificates and postmortem certificates, and between diseases and conditions that caused death. With this results, we suggested some strategy to improve the correctness of death certificate writing practice.
Boo, Kyung Hwan,Lee, Doseung,Nguyen, Quyen Van,Jin, Seong Beom,Kang, Seungtae,Viet, Cao Dang,Park, Se Pill,Lee, Dong-Sun,Riu, Key Zung The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.3
To better understand 20-hydroxyecdysone (20E) fluctuation and accumulation in perennial plant, 20E concentration in individual organs of Achyranthes japonica during reproductive growth stage were analyzed by high performance liquid chromatography (HPLC). Concentrations of 20E in root and floral part were much higher than those in stem and leaf during reproductive growth stage and rapidly increased from flowering stage in August to seed-setting stage in October, and thereafter decreased at the stage of seed maturing in November. In contrast, the 20E concentrations in stem and leaf gradually decreased during reproductive growth. In the analysis of detailed fluctuation of 20E in floral part, the 20E concentration was highest in the seed at the early stage of seed development, compared to flower, peduncle, seed coat, and/or seed in other growth stages, and decreased during seed maturation. The accumulation of 20E in reproductive organs, especially seed and root, suggests that 20E has a defensive role for protection of especially newly developing organs against phytophagous insects.
Construction and Profiling of a cDNA Library from Young Fruit of Satsuma Mandarin
( Kyung Hwan Boo ),( Dae Woon Kim ),( Somi Kim Cho ),( Seong Beom Jin ),( Jae Hoon Kim ),( Hyo Yeon Lee ),( Key Zung Riu ) 한국식물학회 2007 Journal of Plant Biology Vol.50 No.4
To profile gene expression in the early stage of fruit development from ``Nichinan No. 1`` satsuma mandarin (Citrus unshiu Marc.), we isolated total mRNA at 30 d after flowering. A cDNA library was prepared from mature mRNAs and a total of 2350 cDNA clones were partially sequenced. In all, 1914 ESTs were acquired after the removal of the vector sequence and filtering over a minimum length of 150 nucleotides. A total of 763 unigenes, consisting of 138 contigs and 625 singletons, was identified after assembly of those ESTs. According to our homology search with BLASTX against the NCBI database, the deduced amino acid sequences of 253 unigenes were homologous to proteins with known function and 242 unigenes were significantly matched to proteins with putative or unknown functions. The remaining 268 showed no significant similarity to any protein sequences found in the public database with matches higher than an E value of 10-5. The 253 unigenes matched to proteins with known function were then manually assigned to 10 cellular functional categories using a modified MIPS MATDB classification. The expression level of each gene was analyzed based on the redundancy of cDNA clones in each contig that comprised more than 10 ESTs. Here, the most abundant gene expressed in young fruits was for a chitinase precursor. A miraculin-like protein and a lectin-related protein precursor were also abundant.
In vitro plant regeneration of Aster scaber via somatic embryogenesis.
Boo, Kyung Hwan,Cao, Dang Viet,Pamplona, Reniel S,Lee, Doseung,Riu, Key-Zung,Lee, Dong-Sun Japan Society for Bioscience, Biotechnology, and A 2015 Bioscience, Biotechnology, and Biochemistry Vol.79 No.5
<P>We established an in vitro plant regeneration system via somatic embryogenesis of Aster scaber, an important source of various biologically active phytochemicals. We examined the callus induction and embryogenic capacities of three explants, including leaves, petioles, and roots, on 25 different media containing different combinations of α-naphthalene acetic acid (NAA) and 6-benzyladenine (BA). The optimum concentrations of NAA and BA for the production of embryogenic calli were 5.0 μM and 0.05 μM, respectively. Media containing higher concentrations of auxin and cytokinin (such as 25 μM NAA and 25 μM BA) were suitable for shoot regeneration, especially for leaf-derived calli, which are the most readily available calli and are highly competent. For root induction from regenerated shoots, supplemental auxin and/or cytokinin did not improve rooting, but instead caused unwanted callus induction or retarded growth of regenerated plants. Therefore, plant growth regulator-free medium was preferable for root induction. Normal plants were successfully obtained from calli under the optimized conditions described above. This is the first report of the complete process of in vitro plant regeneration of A. scaber via somatic embryogenesis.</P>
Anti-bacterial and Anti-viral Activity of Extracts from Paeonia lactiflora Roots
Boo, Kyung-Hwan,Lee, Do-Seung,Woo, Jin-Kyu,Ko, Seung-Hee,Jeong, Eun-Hyoung,Hong, Quan-Chun,Riu, Key Zung,Lee, Dong-Sun The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
Methanol extracts of Paeonia lactiflora roots (Radix Paeaniae Lactiflora) had antibacterial activity against Bacillus subtilis. These extracts generated superoxide radicals in the B. subtilis lysate. However, the radicals were strongly detoxified by the addition of superoxide dismutase, an indication that superoxide radicals are important in the antibacterial actions of the extracts. The root extracts also showed a pivotal inhibition role in trafficking of viral glycoprotein in virusinfected baby hamster kidney (BHK) cells. The methanol extract effectively inhibited syncytium formation in a concentration-dependent, and it did not affect glycoprotein synthesis. These results suggest that oxygen radical affect the transport process of viral glycoprotein without its synthesis.