세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

금강 수서생태계의 구조와 기능에 관한 연구 : 수질 및 미생물

이규승,김윤석,오덕환,이영하,임항식 忠南大學校 環境問題硏究所 1995 環境硏究 Vol.13 No.-

소백산맥의 주봉인 덕유산에서 발원하여 군산지역에서 황해로 유입되는 금강의 본류는 총 유로연장이 401.4km에 이르는 우리나라 4대강 중의 하나이며, 대전광역시, 충청남북도 및 전라북도 일부지역의 생활용수 및 산업용수의 공급원으로서 대단히 중요한 역할을 담당하고 있다. 1960년대 이후 계속적으로 추진된 경제개발과 공업화 정책에 의해 인구의 도시집중화, 생활수준의 향상등이 이루어지고, 이에 따라 양질의 생활용수 및 산업용수에 대한 수요는 계속적으로 크게 증가하고 있는 실정이다. 그러나 금강의 경우 도시의 생활하수, 공업단지로부터의 공업폐수, 유역의 축산폐수 등에 의해 수질을 비롯한 수환경의 악화가 점차 심화되고 있으며, 이에 따른 수서생태계의 변화도 매우 큰 것으로 보고되고 있다. 특히 최근에 조성된 바 있는 금강 하구둑이 94년 8월부터 가동되고 있고, 또 1995년 이후 금강종합개발이 중류와 하류지역을 중심으로 대대적으로 시행될 예정이어서 금강 본류의 수환경은 장차 많은 변화가 예상된다. 수환경의 변화는 생육하는 생물들의 종조성은 물론 군집의 구조와 기능에 큰 영향을 미치기 때문에 금강 본류의 수서생태계에도 많은 변화가 수반될 것으로 예측되고 있다. 이와 같이 금강본류를 대상으로 하는 사업이 시행된 후 야기되는 수환경의 변화는 장기간에 걸친 조사를 요구하며, 이로부터 얻은 결과를 사업 시행 이전의 조사 결과와 비교함으로써만이 효과적으로 그 경향을 파악할 수 있다. 또한 이러한 비교 분석은 수질관리를 위한 대책 수립과 효율적인 사후 환경관리를 모색하는데 대단히 중요하다. 이에 반하여 지금까지 금강을 대상으로 한 수환경 및 수서생태계의 조사 연구는 단편적인 분야에 대하여 극히 단기간 수행된 것이 대부분이고 정량분석이 아닌 정성분석에만 치중되었기 때문에 금강의 수질 및 생태게보존을 위한 자료로서의 기능을 발휘하지 못하고 있다. 이러한 관점에서 본 환경문제연구소에서는 장기(향후 5년 이상)적인 계획하에 금강본류를 대상으로 수서생태계의 구조와 기능을 조사ㆍ분석하고, 이를 통하여 금강의 수질 및 생태계보존과 수환경관리를 위한 기초자료를 수립하고자 한다. 이와 같이 본 환경문제연구소가 장기적으로 추진하려 하는 금강 수서생태계학술조사는 금강 수서생태계의 현황은 물론 금강을 대상으로 하는 여러가지 형태의 사업에 의한 변화 양상을 정확하게 파악하고, 이에 대한 적절한 대처 방안을 강구하게 할 수 있는 기초자료를 제공하여 줄 것으로 기대된다. 지금까지 금강에 대한 종합적인 생태계 조사가 이루어지지 않고 있음을 고려할 때, 본 학술조사는 장차 금강 수서생태계에 대한 연구의 지침이 될 것으로 기대되며, 이는 본교가 금강 생태계의 보존과 관리를 통하여 지역사회에 기여하는 중추적 학술기관으로서의 위치를 확고히 마련하는 계기가 될 것이다. 본 보고서는 이러한 연구계획의 1차년도 연구결과로서 수질 및 미생물, 수생식물, 어류, 동식물 플랭크톤, 수서곤충을 중심으로 금강 중ㆍ하류 유역 수서생태계를 구성하고 있는 주요 생물군의 구조와 기능을 정량적으로 조사ㆍ분석하는데 그 목적을 두었다. Heterotrophic microbial populations and physicochemical parameters of water samples collected from six stations on the Keum River have been examined from November 1994 to July 1995. The ranges for BOD and COD were 1.5-11.3㎎/L and 2.1-13.7㎎/L, respectively. At the station closest to the estuary, the values of BOD and COD were higher than at other stations, but those of PO₄-P were lower. Metal ions including Cu, Mn, Pb, Cd, and Hg were not detected at all. The population densities of heterotrophic bacteria amounted to 13.7-22.0% of the total bacteria. The densities of coliforms, Salmonella-Shigella, and yeasts were in the range of 49-538 cfu/ml, 16-271 cfu/100ml, and 30-259 cfu/㎖, respectively.

입원환자를 대상으로 한 대집단 싸이코드라마의 치료요인 연구

이후경,김선재,윤성철,이규항,홍향희,함웅 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.2

연구목적 : 본 연구는 개인정신치료와 집단정신치료의 훈련을 받은 정신과 의사라면 누구든지 쉽게 이용할 수 있는 우리나라 입원환자에게 적합한 대집단 싸이코드라마의 모델을 개발하고, 이를 입원환자에게 적용한 후 치료요인을 조사하여, 입원환자 치료에 도움이 되고자 하는 목적으로 본 연구를 시행하였다. 방 법 : 본 연구는 1998년 3월부터 1998년 9월까지 계요병원 입원환자 싸이코드라마에 참가한 모든 환자들과, 보조자아로 참석한 모든 치료팀을 대상으로 하였다. 대상자는 환자가 346명(남자 152명, 여자 194명)이었고, 치료팀은 총 60명이었다. 환자에게는 13가지 치료요인 척도, 싸이코드라마에 대한 일반적인 태도 척도, 도움이 되었던 점에 대해 기술하도록 한 설문지를 사용하였으며, 치료팀에게는 13가지 치료요인과 환자에게 도움이 되었던 점에 대해 기술하도록 한 설문지를 사용하였다. 환자집단은 관객, 보조자아의 역할을 한 환자, 공유(sharing)시간에 참여한 환자, 주인공으로 나누어 치료요인을 비교하였다. 결 과 : 먼저, 환자의 관점에서 본 치료요인은 다음과 같다. 1) 싸이코드라마에서의 역할에 관계없이 거의 대부분의 환자집단에서 실존적 인지와 일차가족집단의 교정적 감정경험을 매우 중시하였다. 2) 주인공, 보조자아의 역할을 한 환자, 공유시간에 참여한 환자와 같이 어떤 형태로든 싸이코드라마에 직접적으로 참여한 환자들은 사회화 기술의 발달을 중시하였으며, 보다 직접적인 형태인 행동으로 참가한 주인공이나 보조자아의 경우 카타르시스를 중시하였다. 3) 주인공을 제외한 모든 환자들은 희망의 고취를 중시하였다. 4) 관객의 경우 치료자와 환자에 대한 동일시와 보편성을 중시하였다. 환자와의 동일시는 공유에 참가한 환자의 경우 특히 중시하였고, 보편성은 보조자아로 참가한 환자의 경우 또한 중시하였다. 다음으로 치료팀이 평가한 치료요인을 환자들이 평가한 치료요인과 비교하면, 치료팀은 환자들보다 집단응집력을 더 중시하였으며, 반면 환자들이 중시한 사회화 기술의 발달과 치료자나 환자에 대한 동일시는 매우 낮게 평가하였다. 결 론 : 환자들의 역할에 따라 세분화하여 조사한 치료요인을 통해 입원환자 대집단 싸이코드라마의 특성과 환자의 역할에 따른 치료요인의 특성을 파악할 수 있었다. 본 모델은 입원환자 치료에 쉽게 적용될 수 있었으며, 전체적으로 많은 환자들에게 도움이 되었다. Objectives : This study was performed to make a model of large group psychodrama suitable for Korean inpatients and easy for psychiatrists trained in the individual psychotherapy and the group psychotherapy to use, to investigate the therapeutic factors after applying this model to inpatients, and then to contribute to the treatment of inpatients. Methods : The study subjects were all the inpatients and the therapists(the auxiliary ego's) who participated in psychodrama from March 1998 to September 1998. The subjects were composed of 346 inpatients (152 males, 194 female) and 60 therapists. To the inpatients 13-Therapeutic Factors Scale, Scale of General Attitude to Psychodrama, and the Questionnaire describing helpful things in psychodrama were administerd. To the therapists 13-Therapeutic Factors Scale and the Questionnaire describing helpful things in psychodrama were administered. The inpatient group were divided into 4 groups(the audience, the auxiliary ego's, the participants in sharing, the protagonists). The therapeutic factors among 4 groups were compared. Results : First, the therapeutic factors described by the inpatients group were as follows : 1) Most inpatients made great account of ‘existential factor’ and ‘the corrective recapitulation of the primary family group’ regardless of their roles in psychodrama. 2) The inpatients who participated in psychodrama directly as the protagonists, the auxiliary ego's, or the participants in sharing made much account of ‘development of socializing techniques’. The inpatients who participated in psychodrama more directly as the protagonists or the auxiliary ego's made much account of ‘catharsis’. 3) All the inpatients except the protagonists made much account of ‘instillation of hope’. 4) The audience made much account of 'identification' and 'universality'. Especially,'identification with members' was much accounted of by the participants in sharing. ‘Universality’ also was much accounted of by the auxiliary ego's. Next, when we compared the therapeutic factors in the inpatient group with those in the therapist group, the therapist group made much account of ‘group cohesiveness’ which the inpatient group made little account of. And the therapist group made no account of ‘development of socializing techniques’which the inpatient group made much account of. Conclusion : By dividing the inpatient group according to their roles, we found the characteristics of the inpatient large group psychodrama and of the therapeutic factors according to the roles of the inpatients. This model could be applied to the treatment for the inpatients easily and was helpful to many inpatients on the whole.

근로청소년의 산업재해와 음주양상과의 상호관계에 관한 산업정신의학적 연구

이길홍,김헌수,이규항,민병근 중앙대부설한국의과학연구소 1983 한국의과학 Vol.15 No.1

만성정신병환자의 재활치료를 위한 주간보호 모델

이규항,김한오,이후경,윤성철,성상경,함웅,윤배중 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 본 연구는 전문병원 위탁형 정신보건센터에서 실시한 주간보호의 전체적인 체계, 운영, 프로그램에 관한 모델을 소개하고, 이 모델을 주간보호에서 시행한 후 그 결과와 시행과정에서 나타난 특징을 분석하여, 우리 실정에 맞는 주간보호 모델을 발전시켜 만성정신병환자의 생활유지와 재활을 촉진시키기 위한 목적으로 실시되었다. 방 법 : 본 주간보호 모델(이하 본 모델이라 함)을 1997년 3월부터 1998년 2월까지 의왕시 정신보건센터에서 총 23명의 환자를 대상으로 실시하였다. PANSS, Quality of Life, 삶의 만족 척도를 주간보호 전과 주간보호 실시 3개월 이후에 조사하였다. 프로그램 유익성 척도, 13가지 치료요인 척도를 주간보호 1개월째와 주간보호 실시 3개월 이후에 조사하였다. 결 과 : 1) 본 모델은 만성정신병환자의 증상을 감소시키고 삶의 질을 향상시켜, 환자의 생활유지 및 재활에 도움을 주었다. 2) 본 모델은 다양한 형태의 집단치료들을 사용하여 만성정신병환자에게 유익을 주었으며, 특히 예술치료와 활동치료가 환자의 신체적, 심리적, 사회적 기능회복을 위해 중요한 역할을 하였다. 3) 본 모델에 작용하는 치료요인의 특징을 보면 주간보호 초기와 후기에 모두 중시된 치료요인은 치료자와의 동일시, 치료자의 조언, 일차가족집단의 교정적 감정경험이었다. 주간보호 초기에 중시된 치료요인은 집단응집력, 이타심이었으며, 주간보호 후기에는 희망의 고취가 중시되었다. 4) 본 모델은 여러 분야의 치료자들과 다양한 치료방법들을 전체적으로 통합하여 사용하는데 유용하였다. 결 론 : 본 모델은 만성정신병환자의 생활유지와 재활을 위해 우리 나라에서 효과적이면서 효율적으로 적용될 수 있을 것으로 생각된다. Objectives : The purpose of this study was to present general system, operation, and program of a day care model managed by a psychiatrist, to evaluate the results of performing this model, and thus to develop a day care model applied to Korean situation appropriately for promoting maintenance and rehabilitation of chronic psychotic patients. Methods : We performed this day care model(hereinafter called as this model) from March 1997 to February 1998. The subjects were composed of 23 psychotic patients. PANSS, Quality of Life Scale, Life Satisfaction Self-Rating Scale were used before beginning this model and after three months of performing this model. Program Helpfulness Scale, 13 Therapeutic Factors Scale were used at one months of day care and after three months of day care. Results : 1) This model helped maintenance and rehabilitation of chronic psychotic patients through reducing their symptoms and increasing quality of life. 2) This model used the various group therapy, especially creative art therapy and activity therapy, played an important role for recovering physical, psychological, and social functions. 3) Important therapeutic factors at early and late period of this model were identification with therapists, guidance of therapists, and the corrective recapitulation of the primary family group. Important therapeutic factors at early period were group cohesiveness and altruism, and at late period instillation of hope. 4) This model was useful for integrating the multidisciplinary therapeutic team and the various therapeutic methods. Conclusion : This model would be applied to day care in this country effectively and efficiently for maintenance and rehabilitation of chronic psychotic patients.

Cu(001) 표면에 성장시킨 Ni 초박막의 구조 연구

閔恒基,李圭燮 弘益大學校 科學技術硏究所 1995 科學技術硏究論文集 Vol.6 No.-

The growth of Ni deposited on Cu(001) surface at room temperature was studied by LEED(Low Energy Electron Diffraction) I/V analyses. For the Ni thin films of thickness from 1 ML(mono layer), the subsurface Ni layer below 1 ML Cu surface layer is found to be the optimum structure. This result is also consistent with the growth mode predicted by the first principles total energy calculation.

습식화학법에 의한 TiO_(2)/수산화아파타이트 생체복합재료의 제조

황규홍,정항철,이종국 조선대학교 생산기술연구소 2003 生産技術硏究 Vol.25 No.1

TiO₂/Hydroxyapatite (HAp,Ca_10(PO₄)_(6)(OH)₂) composite powders were prepared by mixing of the TiO₂ and the HAp powders which had been synthesized through sol-gel, precipitation, hydrothermal and mechanochemical-hydrothermal methods The mixing ratio was fixed with 1 1 ratio (Ti0₂/HAp,wt %). TiO₂/HAp composite powders showed different microstructures depending on their particle size and shape The nano-sized HAp particles were coated on the surface of large TiO₂ particles, whereas they were well mixed and dispersed when both TiO₂ and HAp were nanocrystallites.

근치적 위절제술을 시행한 위암 환자에서 보조요법으로서 5-Fluorouracil, Epirubicin과 5-Epirubicil,Cisplatin의 복합화학요법의 효과 비교

최정혜,안명주,한동수,손주현,전용철,박훤겸,백홍규,이홍기,남영수 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.2

To compare 5-fluorouracil plus epirubicin (FE) to 5-fluorouracil plus cisplatin (FP) chemotherapy as adjuvant therapy for patients with resected gastric cancer. Between August 1995 and March 2000, 46 patients with completely resected gastric cancer received six courses of FE (5-fluorouracil 1000mg/m2/day, D2-D5, epirubicin 70mg/m2, D1) or FP (5-fluorouracil 1000mg/m2/day, D2-D5, cisplatin 70mg/m2, D1) chemotherapy. The 23 patients were assigned to each treatment group. A total of 127 courses of treatment were given both FE and FP group. The FP group tended to show more risk of overall death rate. But there were no differences between FE and FP groups in terms of overall survival or disease-free survival. Both treatment arms were generally well tolerated to chemotherapy. We concluded to be no significant differences between FE and FP groups in terms of overall survival or disease-free survival. To define the efficacy of adjuvant chemotherapy for gastric adenocarcinoma, further prospective randomized trials with large number of patients should be warranted.

정신분열병의 양성아형과 음성아형에 따른 사회적 지지망의 차이

윤성철,이규항,양익홍 大韓神經精神醫學會 1994 신경정신의학 Vol.33 No.2

In this study, positive versus negative symptoms of schizophrenia were examined in relation to social networks. The subjects consisted of 36 positive symptom(PS) and 35 negative symptom(NS) groups of schizophrenics admitted to the Anyang Neuropsychiatric Hospital. There was no significant difference in sociodemographic data(ie. sex, age, education etc) between the two groups. Structurally, the social network of NS group was smaller and contained lesser number of friends and family members than PS group. Furthermore, with respect to the functional aspect of social network. 1) The frequency of contact, comfortability, and material support given by the patients were much less in NS group. However, there was no difference in emotional, material, and informative support between the two groups. 2) In contrast with the PS group, in the 'most important person' category of social network in the NS group, the proportion of 'other people' (fellow patients, hospital staff etc) was much higher than that of friends and family members. 3) All negative symptoms correlated negatively with the size of social network. However, no such relationship existed among PS group. These results indicated close relationships existing between different types of schizophrenic symptoms and the structural and functional aspect of social network.

대입스트레스증후군에 관한 정신의학적 연구

백명기,이길홍,이규항,민병근 중앙대학교 의과대학 의과학연구소 1988 中央醫大誌 Vol.13 No.1

The author attempted to analyze the effect of collage entrance test stress on adolescent‘s mental disorder to secure effective measures coping with collage entrance test stress syndrome. The subjects consisted of 29 third grade high school students and 24 repeatees who were admitted to the neuropsychiatric department of Chung Ang University Hospital and Yongsan Hospital from January 1981 to September 1987. Wide spectra of data from the personal history were obtained through administration of KARI-M questionnaire developed by Korean Adolescent Research Institute, medical records available through psychiatric interview and MMPI and opinions of staff psychiatrists, psychiatric residents, nurses and social workers. The collected data were processed by IBM PC AT computer, using SPSS program, and were statistically analyzed by findings were as follows: 1. Among the total number of psychiatrically hospitalized adolescent inpatients, 11.9% was found to be third graders of senior high school and repeatees, of which 56.6% was found to be hospitalized due to college entrance test stress. A66.7 percent of repeatees, as compared to 48.3% of third graders of senior high school, experienced college entrance test stress. Increment of hospitalization rate due to college entrance test stress markedly changed from 35.7% in 1985-1986 to 70.6% in 1987. 2. The diagnostic categories of examinees were classified as neurotic disorder (30.2%), psychotic disorder (28.3%), adjustment disorder (18.9%), affective disorder (15.1%) and drug dependence (7.5%) etc in the order of frequency. Minor neurotic disorders such as somatization disorder and anxiety disorder were more frequently observed in the third graders of senior high school who were preparing college entrance examination, whereas the control group was more likely to be serious schizophrenic. 3. Third grade examinees with test stress who had been relatively stable in premorbid adaptation level experienced moderate level of stress related especially to changes in school life such as poor academic performance, whereas the control group was found to be suffered from stress arising from changers in family life. 4. The main complaint items of examinees were tension (30.2%), depressed affect (24.5%), suicidal idea (18.9%), persecutory idea (17.0%), headache (15.1%), broken sleep (15.1%), dizziness (15.1%), shyness (15.1%), in these orders, respectively. Also found, among them, were frustration (11.3%), dysphoria (9.4%), loss of interest (9.4%), rage (9.4%), numbness (9.4%), and restlessness (9.4%) etc. In regard to the complaining symptom complex, examinees showed the most frequently somatization (30.2%), and next, depression (18.9%), anxiety (13.2%), sleep disturbance (11.3%) and psychotic symptom complex (11.3%). Examinees with college entrance test stress much more complained of somatizations including headche, abnormal sensation of extremities etc, and anxieties such as tension and restlessness. 5. Examinees with college entrance test stress were more oftenly led to the hospital by their parents and admitted to open wards for about 2 weeks, but were more likely to be rehospitalized due to recurrence of symptoms. It was tended to be more likely that third graders visit the hospital during the first week of March to June, while repeatees were hospitalized most frequently during January and February immediately after the entrance examination. 6. A 34.0 percent of patient‘s guardians showed a lot of resistances to therapeutic milieu, not desiring of exposure of their patient’s demerits, but insisting on their children being treated medically under their consents, further lacking of the insight concerning their children‘s mental problems. This result suggested that the parents tend to deny their children’s problems due to college entrance test stress. It was found that reassurance technique was most effective in order to reduce parent‘s resistances to therapeutic situations. 7. A93.0 percent of examinees showed various resistance including to therapeutic systems. Somatization (22.6%), rebelliousness (18.9%), persistent avoidance (18.9%) and intellectual pursuit (7.5%) etc. In particular, college entrance test stress group showed more often persistent avoidance and intellectual pursuit than control group. 8. The main drugs prescribed to the examinees with college entrance test stress were amitriptyline (20.8%), chlorpromazine (18.9%), and thioridazine (17.0%) etc. Particularly antidepressants such as amitriptyline were more frequently prescribed to patients with college entrance test stress (46.8%) than to the control patients (30.4%). 9. A 67.9 percent of examinees were found to have personality disorders before hospitalization. Significant among these personality disorders were dramatic cluster (37.7%) including histrionic personality, borderline personality, and antisocial personality, anxious cluster (20.8%), an odd cluster (9.4%) such as schizoid personality and paranoid personality. 10. The analysis of MMPI revealed that examinees showed more significant hysterical and hypochondriacal profiles and less significant psychopathic profiles and neurasthenic profiles. These results taken together suggest final conclusions as follows: 1. The college entrance test stress groups show much more depressive trend, various fluctuation in school life, more sensitivity in stress perception, short stay in hospital and higher rate of rehospitalization, even though more stable in premorbid adaptation ability and against anxiety, as compared with control groups. The college entrance test stress patients tend to be hospitalized to open ward most frequently in the first week of March to June which is the first semester of school period. Most patients receive drug treatments including antipsychotic, antimanic, and anticonvulsant drugs along with group therapy, rather than behavior therapy. 2. Repeatees with the college entrance test stress have more seriously psychopathological problems, as compared to the graders showed higher psychotic trend due to family conflict and/or schooling avoidant introverted disposition.