Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

건설공사의 재해예방에 관한 연구

연길환,조희제,임경택,윤석천 忠南大學校 産業技術硏究所 1994 산업기술연구논문집 Vol.9 No.2

This study aims to review the problems and status of accident and construction projects also to find out the ways of preventing the works from accident. It can let them understand the reasons for the accidents and the way of promoting the awareness of safety. For the study the literature and previous studies are reviewed and then the field managers and safety management members were interviewed intent of status of field safety management and accidents data occurred at domestic construction fields.

상아질 접착제의 용매 증발이 접착 효율에 미치는 영향

조민우,김지연,김덕수,최경규 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.5

연구목적: 본 연구는 상아질 접착제의 잔류 용매가 접착 효율에 미치는 영향을 평가하고자 하였다. 연구 재료 및 방법: 본 연구에서는 5세대 2단계 산부식형 접착제와 7세대 단일과정 자가부식형 접착제를 사용하였다. 상아질 접착제의 증발률과 전환률, 적용 후 용매의 공기건조 방법에 따른 미세인장결합강도를 측정하였으며 접착 계면을 FE-SEM을 이용하여 관찰하였다. 결과: 1. 시간에 따라 접착제의 증발률은 증가하나 접착제의 종류에 따라 증가 양상이 서로 달랐다. 2. 대부분 전환률은 증발률에 비례하는 경향을 나타내었다. 3. 공기건조 방법에 따라 결합강도는 대부분 under군, control군, over군 순으로 증가하는 양상을 나타내었다. 4. FE-SEM에서 아세톤을 용매로 사용하는 접착제는 공기건조 방법에 따라 droplet이나 gap이 관찰되었다. 결론: 상아질 접착제의 잔류 용매는 접착 효율에 부정적인 영향을 미치기 때문에, 상아질 접착제의 선택과 사용 시 용매의 종류와 특징에 대한 적절한 이해가 필요할 것이다. Objectives: The purpose of this study is to evaluate bonding efficacy by means of measuring the effect of remained solvent on Degree of conversion(DC) and μTBS and FE-SEM examination. Materials and Methods: Two 2-step total etching adhesives and two single-step self etching adhesives were used in this study. First, volume weight loss of 4 dentin adhesives were measured using weighting machine in process of time in normal conditions and calculate degree of evaporation (DE). Reaction/reference intensity ratio were measured using micro-Raman spectroscopy and calculate DC according to DE. Then 2 experimental groups were prepared according to air-drying methods (under, over) and control group was prepared to manufacturer's instruction. Total 12 groups were evaluated by means of micro tensile bond strength and FE-SEM examination. Results: Degree of evaporation (DE) was increased as time elapsed but different features were observed according to the kind of solvents. Acetone based adhesive showed higher DE than ethanol and butanol based adhesive. Degree of conversion (DC) was increased according to DE except for S³ bond. In μTBS evaluation, bond strength was increased by additional air-drying. Large gaps and droplets were observed in acetone based adhesives by FE-SEM pictures. Conclusions: Additional air-drying is recommended for single-step self etching adhesive but careful consideration is required for 2-step total etching adhesive because of oxygen inhibition layer. Evaporation method is carefully chose and applied according to the solvent type.

증기압 멸균 소독에 의한 치과용 석고의 물성변화

김연웅,이용근,김광만,김경남,조리라,정경호 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.1

Gypsum products have potential contamination by infectious human pathogens, which could be transmitted from patient to the gypsum model via impression materials. Therefore disinfection and sterilization of gypsum products are recommended. Steam sterilization: namely autoclaving is widely available in dentistry. The aim of this study was to investigate degradation of mechanical properties for dental gypsum after steam sterilization. The effect of autoclaving on compressive strength, weight loss and dimensional accuracy of specimens of commercial die stone such as Crystal Rock(CR : Maruishi), Die Keen(DK : Columbus Dental), Resin Rock(RR : Whip Mix), Tuff Rock(TR : Talladium) were estimated, and experimental gypsums{(EP) : 0.5wt% accelerator, 0.02wt% retarder, 0.1wt% surface agent, 0.1wt% Poly Vinyl Acetate} were also measured the above properties after autoclaving. For each group, five cylindrical(6㎜ in diameter and 12㎜ long) specimens made with manufactures water:powder ratio. All specimens were dried in oven until reaching the constant mass. After dried, they were autoclaved at 121℃ for 20 minute, dried again. Compressive strength was measured before and after autoclaving using mechanical testing machine at a cross-head speed of 0.5㎜/min. The dimensional accuracy was estimated by length change of specimens with digital caliper before and after autoclaving. For statistical analysis, ANOVA Tukeys Studentized Range(HSD) test and T test were used. Compressive strength and weight were decreased in all samples after autoclaving. There is significant difference between CR, DK and RR, TR in after autoclaving(p<0.05). EP exhibited the highest compressive strength after autoclaving. The weight was significantly decreased in all group except RR after autoclaving(p<0.05). The dimensional accuracy was not significantly increased in all group except CR. As this result, we found that resin-containing dental stone is more appropriate for autoclaving in dentistry.

2003년 5월의 연무 관측시 에어로졸의 기상·물리·화학 특성

임주연,전영신,조경미,이상삼,신혜정 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.5

Severe haze, mist, and fog phenomena occurred in the central part of Korea during 15~25 May 2003 resulted in poor visibility and air quality. When these phenomena occurred, Korean peninsula was under the effects of anticyclone. The atmosphere was stable, and wind speed was so weak. Under this meteorological conditions, air quality was worse and worse. The characteristics of aerosol in Seoul, Incheon, and Gosan (Jeju) during this period are investigated from the PM_(10). TSP concentrations and aerosol number concentrations. Concentrations of PM_(10) and TSP measured at KMA increased upto 176 and 230㎍/㎥ on 22 May 2003, respectively. Aerosol number concentrations of size range from 0.82 to 6.06 ㎛ increased in Seoul on 17, 19, and 21~24 May 2003, and the concentrations of NO₂ and SO₂had maximum value of 0.165 ppm at Gwanak Mt. and 0.036 ppm at Guro-dong on 23 May 2003, respectively. Result from analysis on heavy metal elements showed high concentrations of Zn, Pb, Cr, Ni, Cu, and Cd during 20~24 May 2003. This event is examined by comprehensive analyses of synoptic weather conditions, satellite images, concentrations of suspended particles and air pollutants, and heavy metal elements.

동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

종격동 종괴를 의심케한 우대동맥궁 1례

김만우,백학연,정춘해,장경식,홍순표,조건국,김종진 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Developmental anomalies of the aortic arch, commonly known as vascular rings, are relatively rare congenital vascular anomalies which can compress the trachea and esophagus. One routine chest film of a patient who was checked and hepatoma-diagnosed in Internal Medicine, Chosun University Hospital left the room for doubt of mediastinal mass. But no subjective symptoms for the mass was told by the patient. This is to report our experience of a patient having right side aortic arch with aberrant left subclavian artery, as proved by diagnosis, but none of congenital heart disease. This right side aortic arch with aberrant left subclavian artery is the most frequent type among right aortic arch, the anomaly of which is usually detected from the incidental suspicion for mediastinal mass at routine chest film; because no symptoms are accompanied therewith.

경부외측종괴의 감별진단 및 접근에 대한 고찰

이경호,박현진,강지연,최희원,조용석,김경원,양수남 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

Most neck masses of specific cause occur in rather predictable locations within typical age groups. This allows a systematic approach to developing a working diagnosis and a differential diagnosis and management plan for the patient presenting with a neck mass. Pathologic masses of the neck will be divided into the following segment: 1. Masses of nonspecific location. 2. Masses in the submandibular region, 3. Masses in the parotid region, 4. Masses in the median-paramedian region, 5. Masses in the lateral neck region. The lateral neck region is the area posterior to the hyolaryngotracheal conduit, below the posterior belly of the digastric muscle and tip of the parotid gland, and extends down to the clavicle. The contents of this region include the large vessels, nerves and greatest number of lymph nodes of the neck, and lymph node metastasis in this region is usually from a primary lesion at the base of the tongue or elsewhere in the oropharynx. Common lateral neck masses include branchial cleft cyst, carotid body tumor, neurogenic tumor, pan-neck infection, cystic hygroma, hemangioma, lipoma and lymphoma. Especially lymphosarcoma. Hodgkin's disease, cystic hygroma, hemangioma and branchial cleft cyst and more common and often manifest in the young patients. Many head and neck disease processes manifest as neck masses. These conditions are treated by surgical excision, except for some inflammatory masses, and often those also must be excised before a diagnosis can be made. We retrospectively reviewed 5-year experiences in the treatment of lateral neck masses at our department to define better clinical characteristics of the masses and the optimal diagnostic and therapeutic approaches to these rare diseases.

Investigation of Quarantine pests inspected by hand-carried plant in Korea international airports and ports during 2017~2020

Kyoung-Yeon Cho,Deuk-Soo Choi 한국응용곤충학회 2020 한국응용곤충학회 학술대회논문집 Vol.2020 No.10

Electrical Characteristics of Poly(ethylene oxide)-urea Complex Films

Cho, Mi-Yeon,Cho, Kyoung-Ah,Kim, Sang-Sig The Korean Institute of Electrical and Electronic 2012 Transactions on Electrical and Electronic Material Vol.13 No.3

The electrical characteristics of complex films composed of poly(ethylene oxide) (PEO) and urea as a function of the urea concentration were examined in this study. Moreover, their structural characteristics were also compared. Depending on the urea concentration, the structural phases were classified as PEO+${\beta}$-phase composite, ${\beta}$-phase+${\alpha}$-phase composites, or ${\alpha}$-phase composite+urea. At urea concentrations below ~0.064 M, the ${\beta}$-phase was dominant in the complex film. Moreover, the conductance increased rapidly with an increase in the urea concentration. For urea concentrations ranging from ~0.064 to ~0.25 M, the ${\beta}$-phase was gradually substituted by the ${\alpha}$-phase. As the film was composed entirely of the ${\alpha}$-phase at urea concentrations greater than ~0.25 M, its conductance was decreased. In this study, the electrical characteristics observed for the different phases are analyzed and discussed.