수학 관련 CD-Rom Title을 활용한 통합 교육활동이 유아의 수학적 문제해결능력과 공간능력에 미치는 영향

윤경선(Youn Kyong-Seon),임영숙(Leem Young-Sook),정상녀(Jung Sang-Nyu) 한국어린이미디어학회 2004 어린이미디어연구 Vol.3 No.-

본 연구의 목적은 수학 관련 CD Rom Title을 활용한 통합 교육활동이 유아의 수학적 문제해결능력과 공간능력에 미치는 영향을 알아보고자 하는데 있다. 연구대상은 만 5세 유아 40명으로서 일주일에 2회씩 총12회 실험에 참여하였다. 실험집단은 수학 관련 CD Rom Title을 활용한 통합 교육활동에 참여하였으며, 비교집단은 수학 관련 CD Rom Title을 제공한 컴퓨터 활동에만 참여하였다. 본 연구의 검사도구는 Ward (1993)가 제작하고 염태옥(2002)이 사용한 수학적 문제해결능력 검사도구와 홍혜경(2001 a)이 개발한 공간능력 검사도구이다. 연구결과 수학적 문제해결능력은 CD Rom Title을 활용한 통합 교육활동 집단의 유아가 매우 의미 있게 높은 것으로 나타났으며 (t=3.798, p<.001) 공간능력에서도 의미 있게 높은 것으로 나타났다(t=2.042, p<.05). The present study examines the effect of integrated activities using CD-Rom Title on children's mathematic solving problem and spatial abilities. Subjects were 40 five-year-old kindergarten children assigned 10 control or experimental groups. The experimental group participated in the integrated activities using CD-Rom Title for forty minutes twice a week for six weeks. Haekyung Hong(2001)'s Spatial Ability Test For Young Children and Mathematic Problem Solving Ability Test used in Taeok Yum(2002)'s study were used for this study. The results showed that the experimental group exhibit more positive change in mathematic problem solving ability and spatial ability score.

말단거대증 환자에서 골교체율과 골밀도

이선화,조보연,이홍규,신찬수,김원배,김성연,박도준,박경수,김희진,김선욱 대한내분비학회 1999 Endocrinology and metabolism Vol.14 No.4

Background: Abnormalities of calcium homeostasis and bone remodelling were known in acromegaly, but controversy concerning the influence of chronically elevated serum growth hormone (GH)/insulin like growth factor-I (IGF-I) concentrations on bone metabolism has been existed. In this study, we assess the effect of chronically elevated serum GH/IGF-I levels on bone metabolism and bone mineral density (BMD) in patients with acromegaly and compare the markers of bone metabolism and BMD of active acromegaly according to gonadal function. Methods: We measured biochemical markers of bone turnover and BMD in 50 acromegalic patients (41 active disease, 9 inactive disease) and 41 control subjects. Results: Active acromegalic patients had significantly higher serum oteocalcin levels (13.8±7.7 versus 6.8±4.7, 6.0±-3.4 ng/mL, p$lt;0.05) and urinary type I cross-linked N-telopeptide (101.7±64.2 versus 49.3±33.3, 56.1±39.4 nM BCM/mM Cr, p$lt;0.05) compared with inactive acromegaly and control subjects. Serum IGF-I levels coed positively with serum osteocalcin levels(r=0.69, p$lt;0.05) and urinary type I cross-linked N-telopeptide (r=0.44, p$lt;0.05). In the female patients with active acromegaly, BMD (T-score) was elevated in the femoral neck(1.00±0.14 versus 0.89±0.12,p$lt;0.05) and trochanter (0.88±0.15 versus 0.77±0.11, p$lt;0.05), whereas BMD of lumbar spine(1.13± 0.17 versus 1.17±0.17, p$gt;0.05) and femoral Ward's triangle (0.78±0.16 versus 0.77+0.13, p$gt;0.05) were not different from those of control subjects. In the patients with active acromegaly, serum osteocalcin levels (16.4±8.8 versus 14.9±10.1 ng/mL, p$gt;0.05) as well as urinary type I cross-linked N-telopeptide (104.8±68.1 versus 122.0±80.3 nM BCM/mM Cr, p$gt;0.05) were not different according to gonadal function. Also, femoral and spinal BMD were not different according to the gonadal function. Conclusion: GH/IGF-I excess increase bone turnover and might achieve a positive bone balance at each remodelling cycle. Markers of bone turnover and BMD of skeletal bone were not in gonadal function in the patients with active acromegaly (J Kor Soc Endocrinol 14:688-697, 1999).

Angiopoietin-like peptide 4 regulates insulin secretion and islet morphology

Kim, Hyun-Kyong,Kwon, Obin,Park, Kyeong-Han,Lee, Kyung Jin,Youn, Byung-Soo,Kim, Seung-Whan,Kim, Min-Seon Elsevier 2017 Biochemical and biophysical research communication Vol.485 No.1

<P><B>Abstract</B></P> <P>Insulin secretion from pancreatic islet β-cells is primarily regulated by the blood glucose level, and also modulated by a number of biological factors produced inside the islets or released from remote organs. Previous studies have shown that angiopoietin-like protein 4 (Angptl4) controls glucose and lipid metabolism through its actions in the liver, adipose tissue, and skeletal muscles. In this present study, we investigated the possible role of Angptl4 in the regulation of insulin secretion from pancreatic islets. Angptl4 was found to be highly expressed in the α-cells but not β-cells of rodent islets. Moreover, treatment of rodent islets with Angptl4 peptide potentiated glucose-stimulated insulin secretion through a protein kinase A-dependent mechanism. Consistently, Angptl4 knockout mice showed impaired glucose tolerance. In the cultured islets from Angptl4 knockout mice, glucose-stimulated insulin secretion was significantly lower than in islets from wild type mice. Angptl4 peptide replacement partially reversed this reduction. Moreover, Angptl4 knockout mice had dysmorphic islets with abnormally distributed α-cells. In contrast, the β-cell mass and distribution were not significantly altered in these knockout mice. Our current data collectively suggest that Angptl4 may play a critical role in the regulation of insulin secretion and islet morphogenesis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Angptl4 is expressed in pancreatic α-cells. </LI> <LI> Angptl4 stimulates insulin secretion from pancreatic islets. </LI> <LI> Angptl4-deficient mice show impaired insulin secretion and dysmorphic islets. </LI> </UL> </P>

A Qualitative Study on Changes in Children’s Social Relations inUnit Block Activities

김영옥,이순례,Kyong-Seon Youn 한국유아교육학회 2006 INTERNATIONAL JOURNAL OF EARLY CHILDHOOD EDUCATION Vol.12 No.1

The present study purposed to examine changes in social relations observed in children’s unit block activities. For this purpose, we collected data through participant observation twice a week for 16 weeks, so a total of 32 times, and analyzed the data. According to the results, unit block activities developed into playing alone, careless suggesting, continuous demanding, demanding and suggesting, meeting with others’ reaction, partial reaction to others’ suggestions, active acceptance of others’ suggestions, initiating and entering into conversation, and talking about subjects. These stages were categorized into playing within the own world, peeping into friends’ world, and sharing a world with friends. Thus, the results of the present study may be used as basic materials for understanding and expanding changes in children’s social relations based on the development of intersubjectivity in unit block activities.

Regulation of Energy Balance by the Hypothalamic Lipoprotein Lipase Regulator Angptl3

Kim, Hyun-Kyong,Shin, Mi-Seon,Youn, Byung-Soo,Kang, Gil Myoung,Gil, So Young,Lee, Chan Hee,Choi, Jong Han,Lim, Hyo Sun,Yoo, Hyun Ju,Kim, Min-Seon American Diabetes Association 2015 Diabetes Vol.64 No.4

<P>Hypothalamic lipid sensing is important for the maintenance of energy balance. Angiopoietin-like protein 3 (Angptl3) critically regulates the clearance of circulating lipids by inhibiting lipoprotein lipase (LPL). The current study demonstrated that Angptl3 is highly expressed in the neurons of the mediobasal hypothalamus, an important area in brain lipid sensing. Suppression of hypothalamic Angptl3 increased food intake but reduced energy expenditure and fat oxidation, thereby promoting weight gain. Consistently, intracerebroventricular (ICV) administration of Angptl3 caused the opposite metabolic changes, supporting an important role for hypothalamic Angptl3 in the control of energy balance. Notably, ICV Angptl3 significantly stimulated hypothalamic LPL activity. Moreover, coadministration of the LPL inhibitor apolipoprotein C3 antagonized the effects of Angptl3 on energy metabolism, indicating that LPL activation is critical for the central metabolic actions of Angptl3. Increased LPL activity is expected to promote lipid uptake by hypothalamic neurons, leading to enhanced brain lipid sensing. Indeed, ICV injection of Angptl3 increased long-chain fatty acid (LCFA) and LCFA-CoA levels in the hypothalamus. Furthermore, inhibitors of hypothalamic lipid-sensing pathways prevented Angptl3-induced anorexia and weight loss. These findings identify Angptl3 as a novel regulator of the hypothalamic lipid-sensing pathway.</P>

Hypothalamic Angptl4/Fiaf Is a Novel Regulator of Food Intake and Body Weight

Kim, Hyun-Kyong,Youn, Byung-Soo,Shin, Mi-Seon,Namkoong, Churl,Park, Kyeong Han,Baik, Ja Hyun,Kim, Jae Bum,Park, Joong-Yeol,Lee, Ki-up,Kim, Young-Bum,Kim, Min-Seon American Diabetes Association 2010 Diabetes Vol.59 No.11

<P><B>OBJECTIVE</B></P><P>The angiopoietin-like protein 4 (Angptl4)/fasting-induced adipose factor (Fiaf) is known as a regulator of peripheral lipid and glucose metabolism. In the present study, we investigated the physiological role of Angptl4 in central regulation of body weight homeostasis.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>Hypothalamic Angptl4 expression levels were measured using immunoblot assay during feeding manipulation or after administration of leptin, insulin, and nutrients. The effects of Angptl4 on food intake, body weight, and energy expenditure were determined following intracerebroventricular (ICV) administration of Angptl4 in C57BL/6 mice. Food intake, energy metabolism, and feeding responses to leptin, insulin, and nutrients were compared between Angptl4-null mice and their wild littermates. Finally, the relationship of hypothalamic AMP-activated protein kinase (AMPK) and Angptl4 was studied.</P><P><B>RESULTS</B></P><P>Hypothalamic Angptl4 expression levels were increased upon food intake or administration of leptin, insulin, and nutrients. Furthermore, central administration of Angptl4 suppressed food intake and body weight gain but enhanced energy expenditure. These effects were mediated via suppression of hypothalamic AMPK activities. Consistently, Angptl4-null mice displayed increased body weight and hypothalamic AMPK activity but reduced energy expenditure. Food intake following a fast was significantly greater in Angptl4-null mice, which was normalized by centrally administered Angptl4. Moreover, anorectic responses to leptin, insulin, and glucose were diminished in Angptl4-null mice. In contrast, Angptl4-null mice were resistant to diet-induced obesity, indicating obesity-promoting effects of Angptl4 under the condition of fat-enriched diet.</P><P><B>CONCLUSIONS</B></P><P>We have demonstrated that hypothalamic Angptl4 is regulated by physiological appetite regulators and mediates their anorexigenic effects via inhibition of hypothalamic AMPK activity. Therefore, Angptl4 appears to have an important role in central regulation of energy metabolism.</P>

Involvement of progranulin in hypothalamic glucose sensing and feeding regulation.

Kim, Hyun-Kyong,Shin, Mi-Seon,Youn, Byung-Soo,Namkoong, Churl,Gil, So Young,Kang, Gil Myoung,Yu, Ji Hee,Kim, Min-Seon Association for the Study of Internal Secretions 2011 Endocrinology Vol.152 No.12

<P>Progranulin (PGRN) is a secreted glycoprotein with multiple biological functions, including modulation of wound healing and inflammation. Hypothalamic PGRN has been implicated in the development of sexual dimorphism. In the present study, a potential role for PGRN in the hypothalamic regulation of appetite and body weight was investigated. In adult rodents, PGRN was highly expressed in periventricular tanycytes and in hypothalamic neurons, which are known to contain glucose-sensing machinery. Hypothalamic PGRN expression levels were decreased under low-energy conditions (starvation and 2-deoxy-D-glucose administration) but increased under high-energy condition (postprandially). Intracerebrovetricular administration of PGRN significantly suppressed nocturnal feeding as well as hyperphagia induced by 2-deoxyglucose, neuropeptide Y, and Agouti-related peptide. Moreover, the inhibition of hypothalamic PGRN expression or action increased food intake and promoted weight gain, suggesting that endogenous PGRN functions as an appetite suppressor in the hypothalamus. Investigation of the mechanism of action revealed that PGRN diminished orexigenic neuropeptide Y and Agouti-related peptide production but stimulated anorexigenic proopiomelanocortin production, at least in part through the regulation of hypothalamic AMP-activated protein kinase. Notably, PGRN was also expressed in hypothalamic microglia. In diet-induced obese mice, microglial PGRN expression was increased, and the anorectic response to PGRN was blunted. These findings highlight a physiological role for PGRN in hypothalamic glucose-sensing and appetite regulation. Alterations in hypothalamic PGRN production or action may be linked to appetite dysregulation in obesity.</P>

렛트에서 단기간의 갑상선 기능 변화에 따른 혈중 렙틴 농도의 변화

김민선,윤초야,조영민,정혜승,신찬수,박경수,김성연,조보연,이홍규,Stephen R. Bloom 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Background: Leptin, an adipocyte derived hormone, and thyroid hormone have similar effects on energy homeostasis, such that a shortage of both hormones is associated with decreased energy expenditure and increased body weight. Therefore, for the maintenance of energy homeostasis may require a close interaction between leptin and thyroid hormone. This study was performed to investigate the change in plasma leptin levels relating to short-term thyroid manipulation causing no significant change in body weight. Methods: Hypothyroidism was induced by surgical thyroidectomy and hyperthyroidism by subcutaneous injection of 50 g of L-T_3/100 g body weight/day, for 5 days, in 6∼8 weeks old male Wistar rats. Body weights and food intakes were monitored daily until sacrifice. Plasma samples were collected, and the thyroid stimulating hormone (TSH), free triiodothyronine (T_3) and leptin levels measured. The plasma leptin levels in rats with hypothyroidism and hyperthyroidism were compared with those of body weights at death and food intakes during the study, atched controls. Results: The rats treated with L-T_3 consumed equal amount of food as freely fed, rats but their final body weights were significantly lower (L-T_3 treated 220.0±1.8 vs. freely fed 226.0±2.0 g, p<0.05). There was no difference in food intake during study, and final body weight, between the thyroidectomised rats and their paired controls (thyroidectomised 220.4±1.7 vs. paired 223.9±4.7g, P=NS). Plasma leptin levels in the L-T3 treated rats were significantly lower than those in freely fed rats (L-T3 treated 1.7±0.1 vs. freely fed 4.8±0.2 ng/ml, p<0.005). Conversely, the thyroidectomised rats had higher plasma leptin levels, compared to those of their paired controls (thyroidectomised 4.8±0.3 vs. paired 1.7±0.1 ng/ml, p<0.005). Conclusion: The Plasma leptin levels in the rats were decreased by short term hyperthyroidism, while they were increased by short term hypothyroidism. These findings suggest that thyroid hormones may affect the production or secretion of leptin

당뇨병성 위무력증 환자에서의 Cisapride 의 효과

김민선,이명철,조보연,이홍규,고창순,민헌기,박경수,박용수,정재훈,김성연 대한당뇨병학회 1993 Diabetes and Metabolism Journal Vol.17 No.4

당뇨병성 말초 신경병증에 대한 PGE1의 효과

김민선,조보연,이홍규,신찬수,고창순,김성연,박경수,박건상,김진성,박형규,김현규,문병술 대한당뇨병학회 1995 Diabetes and Metabolism Journal Vol.19 No.4