침 시술로 발생한 Non-O1, Non-O139 Vibrio cholerae에 의한 패혈증

임태섭,지아영,이중희,장수연,김인수,김영주,김범경,김승업,박준용,안상훈,한광협,김도영 Ewha Womans University School of Medicine 2013 EMJ (Ewha medical journal) Vol.36 No.S

Vibrio cholerae is mainly known to cause gastrointestinal infection after seawater exposure or raw seafood intake. It is rarely reported to cause cellulitis or sepsis, but threre has been no known case after acupuncture. Herein, We report a 56-year-old cirrhotic patient of non-O1, non-O139 Vibrio cholerae septicemia caused by cellulitis of both lower extremities after acupuncture.

랫트의 생체내 카드뮴 단독투여 및 카드뮴과 셀레늄 병용투여에 따른 필수원소의 변화

김동현,변정식,조영채,이범식,김광환,김정윤 忠南大學校 環境問題硏究所 1995 環境硏究 Vol.13 No.-

This study was performed to find out the changes of essential elements as Cu, Fe, Zn, and Ca in blood, liver, kidney and testis of rats. The experimental rats were divided into 4 groups that were consisted of control group(groupⅠ), 3.0㎎Cd/kg group(groupⅡ), 3.0㎎Cd +1.0㎎Se/kg group(groupⅢ) and 3.0mgCd+2.5mgSe/kg group(groupⅣ). Each group was intraperitoneal injection with difference dose of cadmium and selenium such as 3.0mgCd/kg, 3.0mgCd+1.0mgSe/kg and 3.0mgCd+2.5mgSe/kg. Rats were killed at the time of 3 day and 28 day, and Cd, Se, Cu, Zn and Ca concentration in several organs were determined by Atomic Absorption Spectrophotometry and inductively Coupled Plasma. Cadmium content in blood, liver, kidney and testis were significantly increased in the Cd alone injection group than Cd mixed with selenium injection groups. But, copper content were not significantly differences in control and each experimental group. Iron content were significantly increased in the Cd alone injection group than others groups. Zinc and calcium content were significantly decreased in the Cd alone injection group than other groups. On the other hand the essential element content in Cd mixed with selenium injection group were almost equaled with those of control group than Cd alone injection group.

Mineral trioxide aggregate가 인간치수세포에서 유전자 발현에 미치는 영향

김용범,손원준,이우철,금기연,백승호,배광식 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.3

이 연구에서는 mineral trioxide aggregate (MTA)를 in vitro로 인간치수세포에 적용하였을 때 유전자들의 변화를 조사하였다. 실험군은 MTA를 teflon tube (직경 10 mm 길이 2 mm)에 담아 4시간 경화시킨 후HDPCs에 적용하였고, 대조군은 빈 tub만을 적용하였다. 6, 24, 72시간 후 total RNA를 추출하여 microarray를 이용하여 분석하여, 2배 이상 또는 절반 이하의 변화를 보이는 유전자 중 선택적으로 역전사 중합효소 연쇄반응(reverse transcriptase polymerase chain reaction)을 사용하여 발현을 확인하였다. 24,546개의 유전자 중에서 109개의 유전자가 2배 이상 up-regulation되었으며(예. FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, TUFT1) 69개의 유전자가 50%이하로 down-regulation되었다(예. SMAD6, DCN). MTA는 bio-inert한 재료라기 보다는 치수세포에 다양한 경로로 영향을 주는 재료로 사료된다. 특히 치수세포의 분화와 증식에 관여하는 유전자의 변화에 영향을 주며 석회화 과정에 관여하는 유전자의 변화에 직접적인 영향을 주리라 사료된다. This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than twofold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were downregulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bioinert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.

뇌실내출혈로 인한 급성 수두증에서 관찰된 해리증후군

김광기,이경민,이정주,오종배,안태범,정상욱,남현우,박성호 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

Chitosan 濾過 System을 利用한 새로운 中水道 工程의 開發과 溫泉水 淨化에 관한 硏究

김용범,백광욱,안병제,조영주,심재용 서울産業大學校 1994 논문집 Vol.39 No.1

각종 금속이나 중금속을 비롯하여 각종 오염물(SS, COD, BOD, CI 등)을 강력히 흡착제거하는 성능을 가진 chitosan(Cs)응집제와 여과사(SF) 및 활성탄 column(ACC)을 병용하여 hotel잡수와 온천사용수등의 정화실험을 행하여 생물학적 처리과정을 생략할 수 있는 사용수의 재활용 process(Fig. 1)를 개발하였다. 이 process에서 hotel 잡수의 경우 20ppm의 chitosan 투입으로 1차응집 처리하여 약 60%이상의 오염물을 제거한 후 여과사와 활성탄 column을 이용하여 최종적으로는 80~99%의 오염물을 제거할 수 있었다. 또한 온천수의 경우 20ppm의 chitosan 투입으로 1차응집 처리하여 약 50%이상의 COD와 BOD의 제거율을 달성할 수 있었다. 따라서 이러한 process를 산업현장에 적용할 경우 종전의 생물학적 공정을 생략하거나 소규모화하여 보다 간편하며 처리공간이나 경비등을 절감하면서도 사용수를 방류수 또는 재활용수 기준이내로 처리할 수 있음을 확인하였다. 본 연구는 수자원 보호나 수자원 재활용면에서 크게 기여 되리라 믿는다. A simplifid and economic process for recycling the used water discharged from a large scale residence(e. g. hotel and apartment etc.) was developed. The used water was purified through adsorption and flocculation of contaminants by chitosan at the first stage where the average removal rates of COD and BOD of the used water were found more than 60%. The previously treated water through flocculation by chitosan was subjected to filtration systems of a sand filter and an activated carbon column, and the average removal rates of COD and BOD were found more than 80%. The experimental results suggest that the biological process which is very popular in the treatment of the used water for the removal of COD and BOD can be omitted and expected to reduce a spacial and running expenditure. On the other hand, testing the used water discharged from hot springs with chitosan proves a possibility of development of a simple and economical process for the treatment of the used water to the criteria of recycled water quality without any addition of more complicated equipments and facilities.

논에서 물과 양분관리에 따른 메탄(CH_4), 아산화질소(N_2O) 배출 특성

김건엽,박상일,송범헌,신용광 한국환경농학회 2002 한국환경농학회지 Vol.21 No.2

물관리방법을 달리한 상시담수와 간단관개에서 메탄과 아산화질소의 배출 양상과 서로 다른 종류의 유기물원 및 질소비료 시용에 따른 메탄과 아산화질소 배출을 비교하고 저감 할 수 있는 방법을 찾기 위해 본 시험을 실시하였다. 물관리방법으로는 상시담수 (이앙∼출수 35일까지 담수)와 이앙 35일 이후 중간낙수한 간단관개 (중간낙수기간 20일),그리고 시비방법으로는 토양을 검정한 시비량인 토양검정, 토양 검정시비량+볏짚 돈분액비, LCU 완효성비료 그리고 N을 시용하지 않은 대조구 등을 처리하여 메탄 및 아산화질소 배출량을 측정하였다. 메탄 배출량은 간단관개보다 상시담수에서 높았고, 아산화질소 배출량은 이와 반대로 나타났다. 유기물과 비료시용 종류에 따른 메탄과 아산화질소 배출량은 액비와 검정시비+볏짚시용구에서 가장 높았으며, LCU 완효성비료구에서 가장 낮았다. 지구온난화잠재력으로 환산한 온실가스 배출은 물관리 조건에서 상시담수가 간단관개보다 170∼208% 높았으며, 요소를 시용하고 상시담수를 기준 한 것과 간단관개로 물 관리하여 각 처리에 따라 온실가스 배출을 비교하면 요소는 41.4%, LCU 완효성비료 55.8%로 온실가스 배출을 줄일 수 있는 것으로 나타났다. 그리고 비료이용효율은 LCU완효성비료, 액비>검정시비+볏짖>검정시비 순이었다. Emission of methane and nitrous oxide affected by nitrogen fertilizer materials were measured simultaneously in rice paddy fields under flooding and intermittent irrigation in 2000. Studies focused on mitigating CH_4 emission from rice paddy fields are summarized and the possibilities and limits applied to world's rice cultivation are discussed. The mitigation options are water management, soil amendments, organic matter management, different tillage, rotation, and cultivar selection. Altering water management, in particular promoting midseason aeration by short-term drainage, is one of the most promising strategies, although these practices may be limited to the rice paddy fields where the irrigation system is well prepared. The test site was divided into two water managements: a continuously flooded plot which was maintained flooded by constant irrigation from May to September, and an intermittently drained plot in which short-term (20days) draining practices were performed one times during the flooding period. By total emission of GHGs converted by global warming potential (GWP), flooding plots were higher 170~208% than intermittent irrigation plots. For emission of GHGs in fertilizer materials, it was high in the order of Swine slurry>Urea+Rice straw>Urea>LCU. Basing on GHGs emission of urea fertilization under flooding as baseline GWP of urea fertilization and Latex-coated urea under intermittent irrigation showed lower GHGs emission by 41.4% and 55.8%, respectively. In this case fertilizer use efficiency (kg unhulled rice/kg of applied N) were 18.2~20.2 and 18.7~19.0 and 9.3 and 5.8~6.6 for Swine slurry and LCU and Urea+Rice straw and Urea in the continuously flooded and intermittently drained plot.

식이 상태와 카페인 투여가 일회적 운동 시 흰쥐 가자미근의 제 4형 당수송체에 미치는 영향

김광찬,김종오,오명진,윤재석,서태범,윤진환,정일규 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to evaluate the effects of diet condition and caffeine ingestion prior to long distance treadmill running on the GLUT-4 protein expression in Sprague-Dawley rats' soleus muscle. Total is rats were used For subjects and assigned to six groups: Fasting control(FaC group), feeding control(FeC), asting+exercise(FaE), feeding +exercise(FeE), Fasting +caffeine+exercise(FaCE), and Feeding+caffeine+exercise(FeCE). Each animal belongs to fasting group was forced to be in fasting state at least for 14 hours, The food was provided to each rat in feeding group 12minutes prior to treadmill running and the caffeine was ingested through the stainless steel tube 60minutes prior to exercise. All rats in exercise groups were forced to perform treadmill running for B9 minutes at the speed of 18m/min, and 0%grade, immediately after exercise, all animal was anesthetized by Chlorofolm (DC Chemical, Co, Ltd) and about 10ml of blood was drawn from each animal's heart and than soleus muscle was exposed and separated from the rat's leg. we found that the GLUT-4 in feeding groups increased much significantly compared to fasting groups(p<.05). The exercise in fasting state appeared to suppress the expression of GLUT-4 protein different from the exercise in feeding state. Cafreine ingestion prior to treadmill running of 60 minutes induced the significant increase or GLUT-4 protein expression both in fasting or feeding states.

초등학생의 과학에 대한 인지모니터링 기능의 효율성 분석

고광병,고세환,김범기,최관순 한국과학교육학회 2004 한국과학교육학회지 Vol.24 No.6

본 연구는 초등학생의 과학에 대한 인지모니터링 기능이 어느 정도인지, 또한 학년별 수준은 어떠한지를 알아보는데 그 목적이 있다. 연구 대상은 대전시에 위치하는 5개 학교에서 2~6학년까지 각 1개반을 표집하여 총 1001명을 대상으로 2~4학년용인 과학 인지모니터링 검사지Ⅰ(TSCMⅠ)와, 4~6학년용인 과학 인지모니터링 검사지Ⅱ(TSCMⅡ)를 개발하여 검사를 실시하였다. 학년별, 성별 과학 인지모니터링 기능의 효율성에 차이가 있는지를 알아보기 위해 분산 분석과 Scheffe´검증을 실시하였다. 연구 결과에 의하면 2~4학년의 인지모니터링 기능은 63.4%, 4~6학년에서는 57.4%의 효율성을 갖는 것으로 나타났다. 인지모니터링 기능의 효율성을 학년별로 구분 할 때, 3, 4학년은 2학년보다 유의미하게 높게 나타났으며, 5, 6학년이 4학년보다 더 높게 나타났다. 성별로 보면 2, 3학년은 여학생이 남학생보다 높았으며, 4~6학년은 성별 차이가 나타나지 않았다. The purpose of this study was to investigate the effectiveness of cognitive monitoring skills of elementary students on science by the grade and sex. The subject of this study was 1001 elementary students of 2~6 grade from 5 schools. The instrument of study, TSCM Ⅰ(Test of Science Cognitive Monitoring Ⅰ) for 2~4 grade and TSCM Ⅱ for 4~6 grade were developed. The results of this study showed that the effectiveness of cognitive monitoring skills was 63.4% for 2~4 grade and 57.4% for 4~6 grade. The effectiveness of cognitive monitoring skills of 3 and 4 graders was significantly higher than 2, while 5 and 6 was higher than 4 graders. Girls were higher than boys for 2 and 3 grade, but 4?6 graders were not significantly different by the sex.

뒷채움재 특성변화에 따른 교대구조물 해석

정범석,김광수 동의대학교 산업기술개발연구소 2005 産業技術硏究誌 Vol.19 No.-

The compaction-induced earth pressures are much greater than the at-rest values near the surface of a compacted backfill. At large depth, the overburden pressure induced by the weight of the overlying backfill is significantly larger than the vertical stresses applied during compaction. Therefore, in short walls, the magnitude of the total horizontal force on the wall may be controlled by compaction-induced earth pressures. In higher wall, the total horizontal force on the wall may be controlled by the at-rest pressures for normally consolidated soil backfill. From a series of bridge abutment analyses, various sets of design parameters versus backfill height relationships for different densities and different internal friction angles of the backfill are developed. The influence of changing the backfill material on the design factors for the type of bridge abutment increases with increasing wall heights. Depending on the properties of the backfill material, a certain load combination can give harmful effect to the wall structure specially in the poorly designed drainage systems. The structural design must be adjusted by several methods until the analysis results matched the target values of the design parameters from each code. Therefore, use of new backfill material for the type of bridge abutment is recommended for a close examination of its properties and economical advantages.

정형외과 영역에 있어서 Ketorolac Tromethamine과 복합제(Aspirin/Acetaminophen/Caffeine Anhydrous)와의 비교 임상적 연구

이광진,임상덕,김석범 최신의학사 1989 最新醫學 Vol.32 No.2