췌장암에서 Cyclooxygenase - 2 발현의 역할

김은경,이용욱,이규택,최규완,박동일,백승운,이준행,이종균,고광철,이풍렬,이종철,김재준,김완선,공구 대한소화기학회 2001 대한소화기학회지 Vol.38 No.3

Background/Aims: Accumulating evidence suggests that the use of NSAID may reduce the incidence of colorectal cancer. The likely mechanisms of these effects by NSAID is cyclooxygenase-2 (COX-2)-related inhibition of tumor proliferation and induction of apoptosis. The aim of our study was to examine possible roles and clinical significance of COX-2 expression in pancreatic cancer. Methods: Seventy-two pancreatic adenocarcinoma tissue specimens were obtained from surgical resection. After the immunohistochemical staining of the specimens, we examined proliferation activity (assayed by Ki-67 expression), apoptosis (by TUNEL stain), and microvessel density (by CD34 expression). We also investigated the relationship between the immunohistochemical expression of COX-2 and various clinicopathological characteristics. Results: The COX-2 positive rate in pancreatic epithelial cells was 41.7%. Proliferation index (PI) was significant higher in COX-2 positive specimens comparing to negative specimen (p=0.015) and the increase in intensity of COX-2 expression correlated with increasing PI (p=0.036). Apoptotic index (AI) was significantly higher in positivee COX-2 expression than in negative expression (p=0.044), but there was no significant difference in AI/PI between the COX-2 positive and negative specimens (p=0.44). The expression of COX-2 protein did not correlate with microvessel density, sex, age, differentiation, tumor size, stage, metastasis or patients survival. Conclusions: The expression of COX-2 enzyme in pancreatic cancer contributes to tumor proliferation, but is not related to apoptosis, angiogenesis or clinical characteristics. Further study is needed to examine the clinical usefulness of NSAID and COX-2 selective inhibitors.

간세포암과 주위 조직에서의 Transforming Growth Factor-beta 1과 그 수용체의 발현

최규완,백승운,최문석,김소정,이준혁,고광철,이풍렬,이종철,박상종,안병훈,김재준 대한소화기학회 2001 대한소화기학회지 Vol.37 No.4

Background/Aims: Transforming growth factor-beta 1 (TGF-β1) is a potent inhibitor of hepatocyte proliferation and its receptor is known to be involved in the carcinogenesis. This study was designed to compare the expression levels of the TGF-β1 and its receptor in neoplastic cells and its surrounding tissue and to assess their role in hepatic carcinogenesis. Methods: We measured the levels of TGF-β1 mRNA and TGF-β1 receptor II mRNA by semiquantitive RT-PCR for the tumor and its surrounding tissue of 30 patients with hepatocellular carcinoma (HCC). We analyzed the relationship between the expression of TGF-β1 mRNA, TGF-β1 receptor II mRNA and clinicopathological characteristics of HCC. Results: The expression of TGF-β1 mRNA showed no difference between HCC and its surrounding tissue, but the expression of TGF-β1 receptor II mRNA was lower in HCC than in its surrounding tissue (2.47±1.08 vs 3.53±1.50, p=0.00). There was no difference in the expression level of TGF-β1 mRNA and TGF-β1 receptor II mRNAo the clinicopathological characteristics of HCC. However, the ratio of TGF-β1 receptor II mRNA in HCC to that in surrounding tissue was more decreased in HCC with portal vein invasion (0.49±0.22 vs 0.77±0.21, p=0.01). Conclusions: Our data suggest that the loss of TGF-β1 receptor II may play an important role in carcinogenesis and tumor progression of HCC.

Glutaraldehyde 와 자동 세척기를 이용한 내시경 소독방법의 임상에서의 유용성

김영호,손희정,윤성원,최규완,이남용,백승운,이준행,고광철,이풍렬,이종철,송재훈,안병훈,김재준,최원혁,홍일철 대한소화기내시경학회 2001 Clinical Endoscopy Vol.22 No.6

Background/Aims: Safety of endoscopic procedures has been a major issue over the last 10 years. Most endoscopy units use 2% glutaraldehyde and automated endoscope reprocessors (AERs) for disinfecting gastrointestinal endoscopes. We attempted an in-use evaluation of the current reprocessing procedures. Methods: Thirty flexible endoscopes were randomly collected just after upper endoscopic examinations and were disinfected using 2% glutaraldehyde in an AER. Cultures were taken from biopsy channels (S-l), tip of the insertion tubes (S-2), umbilical cords (S-3), and angulation knobs (S-4). Results: In 63,3% (19/30) of endoscopes, there was no microbial contamination after disinfection procedures. The culture positive rates of S-l, S-2, S-3, and S-4 samples were 20,0%, 0.0%, 3.3%, and 20.0%, respectively. Microorganisms of 13 species were identified, but there was no pathogen related with reported infectious complications after endoscopic procedures. Conclusions: Current disinfection procedure using 2% glutaraldehyde and an AER appears to be very effective in decontaminating patient-used endoscopes. Low level microbial contamination of endoscopes after conventional reprocessing methods may not impose great risk on patients.

역류성 식도염 환자에서 Helicobacter pylori 치료 1 년 후 증상과 내시경적 변화

김영호,손희정,최규완,박동일,백승운,이준행,고광철,이풍렬,이종철,임윤정,김재준 대한소화기내시경학회 2001 Clinical Endoscopy Vol.24 No.1

광섬유 센서를 이용한 절연유의 방전신호 및 감쇄특성 측정

김태영,김상준,남진호,서광석,이수묵 고려대학교 공학기술연구소 1999 공학논문집 Vol.37 No.1

This paper presents the preliminary results on the application of optical fiber sensor(OFS) for the measurements of discharging signal and degradation in insulation oil such as transformer and silicone oil. An OFS system using a Mach-Zehnder interferometry technique was built to detect attenuation of acoustic signal produced by discharging. Simultaneous measurements were made of electrical signal and OFS signal from discharge. It was found that the arcing signal produced by needle-sphere electrode system could be detected by OFS installed in an oil bath. With increasing OFS distance from an arcing spot, the attenuation of acoustic signal became greater. From the results of sound attenuation for various insulation oils, the linear relationship was observed between the attenuation signal and viscosity in log scale. Details of these results are discussed.

신장이식에 대한 마취경험

김종래,박광원,문영삼,정인석,손영은 대한마취과학회 1982 Korean Journal of Anesthesiology Vol.15 No.1

활동성 크론병에서 초기 스테로이드 치료 반응에 따른 임상적, 생화학적, 병리학적 인자들에 관한 연구

김영호,장재권,김지은,최규완,박동일,도재혁,백승운,고광철,이풍렬,이종철,현재근,안병훈,김재준,류민규 대한소화기내시경학회 2001 Clinical Endoscopy Vol.22 No.6

Background/Aims: The predictory factors of the response to initial steroid therapy in active Crohn's disease has been controversial in numerous literature reviews. We evaluated any predictory faetor of the response to initial steroid therapy in active Crohn`s disease patients, Methods: The medical records of 32 patients with active Crohn`s disease who clinically responded to oral steroid therapy were retrospectively reviewed. The steroid responsive group was defined as the one showing maintenance of response for more than one month from steroid withdrawal and the steroid dependent group as the one showing relapse or exacerbation during steroid tapering or within 30 days from steroid withdrawal. The clinical, biochemical, and pathologic factors were evaluated. Results: There were 22 male and 10 female patients. The mean age was 28.9 years. The number of steroid responsive and dependent group was 22 (68.8%) and 10 (31.2%), respectively. There were no significant differences between these two groups in age, sex, time to diagnosis, perianal lesion, extent of disease, extraintestinal manifestations, presence of granuloma, presenting features, hemoglobin, ESR, and CRP, except serum albumin level. Conclusions: Serum albumin level was significantly lower in steroid dependent group than steroid responsive group, reflecting severe inflammation in steroid dependent group.

고에너지 전자빔투사방법을 사용한 TiB2/Ti 표면합금화 재료의 제조 연구

구양모,이성학,어광준,김낙준,오준철 대한금속재료학회(대한금속학회) 1998 대한금속·재료학회지 Vol.36 No.12

The present study is concerned with the fabrication and the microstructural analysis of a TiB₂/Ti surfacealloyed material fabricated by high-energy electron beam irradiation. The mixture of TiB₂ powders and flux was deposited on a Ti-10V-2Fe-3Al alloy substrate, and then electron beam was irradiated on these mixture using an electron beam accelerator. The melted region of about 1.5 ㎜ thickness was homogeneously formed without pores or cracks, and was composed of primary and eutectic TiB borides in the β phase matrix. The formation of these TiB borides greatly improved hardness, especially high-temperature hardness up to 450℃. These findings suggested that the surface-alloying method using high-energy electron beam irradiation was economical and useful to the development of new advanced materials with improved high-temperature properties.

허혈성 대장염의 임상 양상과 예후 인자

이미숙,손희정,강태욱,최규완,박동일,백승운,이준행,고광철,이종철,임윤정,이풍렬,김갑철,김재준 대한소화기내시경학회 2001 Clinical Endoscopy Vol.22 No.2

Background/Aims: Colonic ischemia is recognized as the most common vascular disorder of the intestines in the elderly. The severity of the disease may vary considerably with the prevalent benign transient forms, being amenable to conservative treatments and the severe gangrenous forms, requiring surgery. Correct diagnosis is usually made after the ischemic episode is over and colonic ischemia is frequently misdiagnosed. The aim of this study is to identify the clinical patterns and prognostic factors of ischemic colitis. Methods: We found 60 patients with nonoccusive ischemia of the large intestine, We analyzed age, sex, symptoms, signs, associated disease, location, endoscopy, hematologic, histologic, radiologic finding and treatment, retrospectively. Results: The mean age was 60.2±6.3 years. The most common symtoms were abdominal pain or bloody diarrhea. The most common sign was diffuse abdominal tenderness. The most common hematologic findings were anemia and leukocytosis. Associated medical illnesses wer diabetes, rhematoid arthritis and cerebrovascular accident, et al. Both computed tomography (CT) and endoscopy were useful diagnostic tools. The most common involved site was sigmoid colon. 46 patients were treated conservatively and 14 patients were managed surgically. Conclusions: Male gender, those with rebound tenderness, severe anemia, right or total colon involvement as well as rheumatoid arthritis had a poor prognosis. Both CT and endoscopy were useful in predicting prognosis.

최근 국내외에서 육성된 무궁화 127 품종 중 조경적 활용가치가 높은 유망품종 선발

김광호 ( Kim Kwang-ho ),이춘석 ( Lee Chun-suk ),강호철 ( Kang Ho-chul ) 한국전통조경학회(구 한국정원학회) 2016 한국전통조경학회지 Vol.34 No.3

본 연구는 조경수용 무궁화 우수품종 보급을 위한 유전자원 수집과 특성을 평가하기 위해 최근 국내외에서 육성된 127품종의 1년생 접목묘를 이용하여 2014년부터 2015년까지 2년 동안 생육 및 형태적 특성조사를 실시하였다. 수집된 품종 중 화색 및 꽃이특이한 품종을 조사하여 무궁화를 조경수로 활용도를 높이는데 기여하고자 하였으며 얻어진 결과는 다음과 같다. 최근 국내외에서 육성된 품종들의 화색별 분류로는 크게 분홍색 단심(pink color with red eye spot), 흰색 단심(white color withred eye spot), 붉은 자주색 단심(purplish red color with red eye spot), 분홍보라색 단심(violet pink color with red eye spot), 심홍색단심(crimson color with red eye spot), 흰색 바탕에 붉은색 무늬의 이중색(asadal), 흰색(white color), 푸른색(blue color) 등 총 8종류로 분류되었다. 화색이 붉은 자주색으로서 적색이 강한 꽃으로 국내에서 육성된 품종으로 ‘광명’, ‘난파’, ‘노스페이스’, ‘불새’, ‘비단’,‘송암’, ‘영창’, ‘적퍼소’, ‘키호’, ‘탐라’, ‘화수레드’, ‘환희’ 등이 있었으며, 국외에서 육성되어 국내에 도입된 품종으로는 ‘Aphrodite’,‘Dr. Uemoto’, ‘Freedom’, ‘Pink Cup’, ‘PS 80-1’, ‘Purpureus Variegatus’, ‘Red Giant’, ‘Woodbridge’ 등이 있었다. 화색이 분홍보라색으로서 특이한 품종으로는 ‘꼬마’, ‘도투락’, ‘명미’, ‘병화’, ‘산처녀’, ‘태화’, ‘Hikari-hanagasa’, ‘Little Kim Violet’ 등이 있었다. 화색이 심홍색으로 기존의 꽃에서는 볼 수 없는 특이한 품종으로 ‘종무’와 ‘Ruffled Satin’ 등이 있었다. 최근 국내외에서 육성된 품종중 조경수용 소재로 보급할 수 있는 품종중 기존 품종에 비해 꽃의 크기가 큰 품종으로 무궁화 종간교잡종인 Hibiscus hybrid ‘Daewangchun’, ‘Daeil’,‘Jina’ 등이 있었다. 반면 꽃이 작고 조경적 가치가 높은 품종으로 ‘꼬마’, ‘미백’, ‘안동’, ‘릴킴’, ‘은하수’ 등이 있었다. 백단심계 무궁화‘꼬마’ 품종은 수고가 작은 왜성형으로 꽃의 크기가 5.2cm로 작은 소형화 품종으로 나타났다. 기존 품종에 비해 단심의 길이가 긴품종으로 ‘훈장’과 ‘하이리레드’가 있었다. 홍단심계 무궁화 ‘훈장’은 꽃의 크기는 12.0cm로 중간크기이나 단심길이는 4.8cm로 지금까지육성된 품종중 가장 긴 품종이었다. 또한 홍단심계 무궁화 ‘하이리레드’의 단심길이 역시 4.2cm로 꽃잎 길이에 비해 단심의 길이가 길어 조경적 가치가 높은 것으로 판단되었다. This study was carried out to characterize 127 recently developed cultivars of Rose of Sharon (Hibiscus spp.) in Korea and foreign Countries for landscape uses. The examined factors were growth characteristics such as tree height of a 1-year grafted plant(cm), plant type, growth habit, leaf characteristics such as shape and size, flower characteristics such as color, shape, size, and red eye during 2014 and 2015 for landscape uses. The results are obtained as follows; In the results of flower color of the 127 recently developed cultivars, pink color with red eye spot, white color with red eye spot, purplish red color with red eye spot, violet purple color with red eye spot, crimson color with red eye spot, asadal, blue color with red eye spot, and white color were distributed. In the flower characteristics, Hibiscus hybrid ‘Daewangchun’ had the largest flower size of 16.0cm out of the 127 cultivars. H. syriacus ‘Antong’, ‘Lil Kim’, and ‘Ggoma’ were cultivars with smaller flowers than other cultivars. H. syriacus ‘Hunjang’ had largest red eye, of 5.2cm of length compared to the other cultivars. The cultivars with unique flower color for landscape uses are H. syriacus ‘Kwangmyung’, ‘Nanpa’, ‘North face’, ‘Bulsae’, ‘Bidan’, ‘Songam’, ‘Youngchang’, ‘Jukpeoso’, ‘Kiho’, ‘Tamla’, ‘Hwasoored’ and ‘Hwanhee’. These flowers had a purplish red color and were developed in Korea. H. syriacus ‘Aphrodite’, ‘Dr. Uemoto’, ‘Freedom’, ‘Pink Cup’, ‘PS 80-1’, ‘Purpureus Variegatus’, ‘Red Giant’, ‘Woodbridge’ also had unique flowers with a purplish red color and were introduced from foreign countries. In addition, cultivars with violet pink flowers were H. syriacus ‘Ggoma’, ‘Doturak’, ‘Myungmi’, ‘Byunghwa’, ‘Sancheonyu’, ‘Taehwa’, ‘Hikari-hanagasa’, and ‘Little Kim Violet’. ‘Jongmoo’ and ‘Ruffled Satin’ had flowers with crimson color. Therefore, the new cultivars with unique flower colors were a promising cultivars to a woody landscape plant. Cultivars with large flower sizes were Hibiscus hybrid ‘Daewangchun’, Hibiscus hybrid ‘Daemang’, and Hibiscus hybrid ‘Jina’. H. syriacus ‘Ggoma’, ‘Mibeak’, ‘Antong’, ‘Lil Kim’, and ‘Eunhasu’ had small flower sizes. Cultivars with long red eye were H. syriacus ‘Hunjang’ and H. syriacus ‘Hi Lea Red’. Therefore, the new cultivars, Hibiscus hybrid ‘Daewangchun’, Hibiscus hybrid ‘Daemang’, Hibiscus hybrid ‘Jina’ with large flower sizes, H. syriacus ‘Ggoma’, ‘Mibeak’, ‘Antong’, ‘Lil Kim’, and ‘Eunhasu’ with small flower sizes, H. syriacus ‘Hunjang’ and H. syriacus ‘Hi Lea Red’ with long red eye, were promising cultivars to a woody landscape plant.