마그네트론 스퍼터용 20kW급 플라즈마 전원장치 개발

서광덕,김상훈 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A

This paper describes a power supply aimed at the production of plasma and its control method for a magnetron sputter in thin film coating process of PVD(Physical Vapor Deposition). Plasma load changes its impedance characteristic easily according to operating conditions and frequently produces electric arc. So, in this paper, a plasma power supply with improved output control performance in the transient state for the plasma load is presented. Also, it includes a strategy that can detect arc rapidly and reduce arc energy effectively into a load. The validity of the proposed power supply through experimentation on 20kW system was proved.

효과적인 SCM 구축을 위한 Positioning & Strategy에 관한 사례연구

서장훈,양광모,박명규 한국생산성학회 2004 生産性論集 Vol.18 No.2

Recently, an enterprise's survival strategy is a difficult task, because the competition of a business is more complex ever. The research aims to demonstrate the dynamic structural change in a conceptual point of SCM positioning & strategy. But it has many problems since it is difficult and wide to define. Therefore, the Manager don't aware a total executable implementation even though the enterprise knows necessity of SCM. This paper focuses on a proposal of alternatives with reasonable positioning like most of enterprises willing to overcome such as carelessness and unready strategy of investment, additionally, the thesis should effort to find an element through an analysis of cases, a statistical method of effective SCM, and actual survey to propose an alternative between Positioning & Strategy in Enterprise SCM.

군위지역 오이 시설 재배지의 토양특성 변화

이동훈,최충렬,김광섭,김평열,서정우,박만 경북대학교 농업과학기술연구소 2006 慶北大農學誌 Vol.24 No.-

군위군 오이재배지역을 정밀토양도를 이용하며 토양특성을 조사하구 군위군 노지 및 시설오이 재배지토양의 물리화학적 특성을 조사하여, 연작과 관행적인 토양관리로 인하여 저하된 토양의 이화학적 특성을 오이재배에 적합한 토양으로 개량하기 위한 기초자료로 이용하고자 토양조사를 실시하였다. 조사지역은 군위군 군위읍 외랑리 오이재배단지로서 주요토양통은 토양토에 나타난 단북통과 신정들이 가장 넓게 분포하였으며, 점토함량은 시설재배지내 토양의 경우 양토이었으며, 시설재배지의 외부 토양의 경우 23.8%로 높게 나타났다. 오이 재배지 토양의 화학적 특성 결과에 대한 오이재배지 토양의 적정범위와비교하여 보면 일반적인 화학적 성질에서 서는 유효인산의 함량이 적정범위보다 높은 것을 알 수 있으며, 치환성 양이온 함량은 시설재배지의 외부토양은 적정범위에 속하나 시설재배지 내부토양은 모두 높은 것으로 나타났으며, 최상부 토양은 매우 높으나 l0cm이하는 깊이에 따라 뚜렷한 차이는 없었다. 오이 재배지 토양 중 중금속 함량은 모두 기준치보다 높게 나타났는데, 구리의 함량이 시설재배지가 노지재배지보다 높게 나타났다. The soils of plastic film houses in which intensive cultivation takes place suffer from the serious problems such as severe salinity and accumulation of heavy metals etc. The objective of this research was to examine the change in soil properties with repeated cucumber cultivation under plastic film house at Gunwi-Gun area. The soils were classified mainly to Danbuk and SinJung series. Clay content is lower in the soils of plastic film house than in the field soil. Available P205 and exchangeable cations were accumulated at the level higher than the optimum range. The content of extractable heavy metals were higher in the soils of plastic film house than in those of the field. For optimum yield, much attention should be paied to the management of soils by the pre-soil survey.

Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예

김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5

We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)

녹차 및 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용

임성훈,서정순,윤영주,김광원,유소영,김화숙,국중기,이병래,차종희,박재윤 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.5

본 연구는 치아우식증에 관련된 Mutans streptococci 표준균주 및 임상분리 균주의 성장억제를 유도할 수 있는 천연 생약추출물을 검색하기 위하여 실시되었다. 녹차추출물은 CHMC-2032를 사용하였으며, 결명자 추출물은 50% 에탄올을 이용하여 얻었다. 이들 추출물의 Mutans streptococci 표준균주 및 교정환자들에서 브라켓과 치면 사이의 경계부에서 분리된 각각 10 균주씩의 S. mutans 및 S. sobrinus에 대한 최소성장억제농도를 액체배지 희석법으로 구하였다. 그 결과 CHMC-2032의 S. mutans 및 S. sobrinus의 표준 균주, S. sobrinus의 대부분 임상분리 균주에 대한 최소성장억제농도는 5 mg/ml이었다. 그러나 결명자 추출물에 의한 S. mutans 및 S. sobrinus에 대한 세균 성장억제 효과는 미미하였다. 본 연구 결과 치아우식증의 예방적 측면에서 결명자차보다는 녹차를 마시는 것이 유리하며, CHMC-2032를 이용하여 구강양치용액을 제조하여 사용할 경우 교정환자를 포함하여 대부분의 사람에게서 치아우식증 예방 효과가 있으리고 추정된다. Mutans streptococci is the major causative factor in dental caries. Especially, orthodontic patients with fixed appliance are a risk group for dental caries. Because fixed appliances attached on teeth may change the environment of dental plaque, the enamel decalcification or dental caries around the bracket and band is a major side effect of orthodontic treatment. The aim of this study was to search plant extracts that have antimicrobial effect on mutans streptococci. Seed-extract of Casio tora were prepared with ethanol and CHMC-2032, the leaf-extracts from Camellia sinensis extract, was obtained extract, 2 type strains and 20 clinical isolates of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces in the orthodontic patients were used in this study. The minimal inhibitory concentration of CHMC-2032 was 5 mg/ml on the S. mutans KCTC 3065, S. sobrinus KCTC 3088, and 8 clinical isolates of S. sobrinus. However, there was no antibacterial effect of seed-extract of C. tora on mutans streptococci. These data suggest that green tea may be more effective than the tea prepared from C. tora in the prevention of enamel decalcification or dental caries around brackets.

고혈압 환자에서 Leukoaraiosis 정도와 망막증과의 연관성 : 예비연구 Preliminary Results

신동익,한현정,서대희,이광훈 關東大學校 醫科大學 醫科學硏究所 2002 關東醫大學術誌 Vol.6 No.1

Changes in the cerebral white matter (leukoaraiosis;LA) are detected with increasing frequency by computed tomography (CT) and magnetic resonance imaging (MRI) in elderly patients with diabetes mellitus and hypertension. The purpose of this study is to determine whether the severity of retinopathy is correlated with degree of LA in patients with hypertension. Twenty-three consecutive patients with patients with hypertension admitted in the department of neurology were evaluated for degree of LA and severity of retinopathy. 8patients were 15female. The age distribution was between 52 and 85 years(mean 69.1years). The duration of hypertension was between 1 and 30 years(mean8.2 years). The degree of LA was correlated with severity of hypertensive retinopathy(p<0.05), especially in relatively young patient, short periods of disease, and female paitents(p<0.05). We can make a rough estimation of degree of LA with fundus examination without CT or MRI evaluations in hypertensive patients, especially in relatively young and female patients with short durations of disease. However, more broad study should be made for these value and relationship.

제1형 당뇨병 환자에서 췌도세포 동종이식의 반복시행

양태영,정인경,서인아,오은영,조건영,오승훈,김성주,정재훈,민용기,이명식,이문규,김광원,도영수,주성욱 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.4

연구배경:췌도세포 이식은 시술이 간편하고 안전하기 때문에 반복이식이 가능하여 그 동안 여러 센터에서 반복이식의 성공을 보고한 바 있다. 국내에서도 본 병원을 비롯하여 몇몇 센터에서 췌도세포 이식이 활발히 시도되고 있으나 사람에서의 동종이식 및 동종 이식의 반복시행은 보고된 바 없는 실정이다. 저자 등은 국내 최초로 췌도세포 동종이식을 시행하여, 혈청 C­펩타이드가 증가하고 인슐린 요구량이 감소하였으며, 혈당농도와 당화혈색소의 안정을 보였다. 그러나 이식 70일 후 다시 인슐린 요구량이 증가하고 C­펩타이드가 감소하여 췌도세포 이식을 다시 시행한 바 있다. 2차 이식 후 70여일이 지난 현재 다시 혈청 C­펩타이드가 증가하고, 인슐린 요구량이 더 감소되어 췌도세포 이식에서 반복 시행의 유용성을 보고하는 바이다. 방법:환자는 32세 남자로 17년전 당뇨병 진단 후 인슐린 치료 중이었으며, 3년전 부터는 만성신부전증으로 혈액툭석을 하고있었다. 1999년 12월 25일 신장이식을 하였고 3일 후 췌도이식을 하였으며, 두번째 이식은 70일 후 시행하였다. 췌도분리는 변형된 Recordi방법과 비연속성 자당 농도차(discontinuous density gradient)를 이용하였으며 분리한 췌도는 배양 후 환자의 신장기능이 정상화되고 미생물학 검사에서 음성을 확인한 후 경피경간으로 간문맥을 접근하여 16G 폴리 에틸렌 카테터를 이용하여 간실질에 이식하였다. 결과:1차 췌도이식시 순수분리전 췌도수는 210,000개, 순수분리후 획득한 췌도수는 90,000개, 순도 95%, 세포양 1.0mL 이었고, 2차 췌도이식시 순수분리전 췌도수는 420,000개, 순수분리후 획득한 췌도수는 370,000개, 순도 95%, 세포양 1.5mL 이었다. 1,2차 모두 간문맥을 통해 약 20분에 걸쳐 간실질에 주입하였다. 췌도이식전 인슐린요구량은 75∼75U/일, HbA1e 8∼10%, C­펩타이드 0.6ng/mL 였으며, 1차 췌도이식수 7일째 인슐린 요구량은 40U/일, C­펩타이드 1.5ng/mL, FPS 109mg/mL 였고, 40일 추적관찰 후 인슐린 요구량이 36U/일, C­펩타이드 1.8ng/mL, HbA1e 6.5∼7.0%로 안정되었다. 그러나 이식 50일째부터 인슐린 요구량이 50∼56U/일, C­펩타이드 0.6ng/mL, FPS 130∼200mg/dL로 혈당 조절이 불안정하여 다시 췌도이식을 시행하였다. 2차 이식후 50일이 경과한 현재, 인슐린 요구량은 26U/일, C­펩타이드 1.8ng/mL, FPS 90∼120mg/dL로 다시 안정되었다. 결론:췌도이식은 반복이식이 가능하며, 본 환자의 경우 인슐린요구량 감소, 혈당의 안정화 및 C­펩타이드가 상승하여 이식한 췌도의 기능을 확인할 수 있었고, 향후 스테로이드 등 면역억제가 유지 용량으로 감량되면 인슐린 요구량은 더 감소될 것으로 기대된다. Over the past 20 years, significant advances have been made in human islet transplantation. However, cases of prolonged insulin independence after islet allotransplantation have rarely been reported and over time, a slight, gradual decrease in insulin secretion appears to occur, as suggested by the lower C-peptide. Although preliminary clinical success achieved over the past few years has been considerably higher with whole pancreatic transplant than with isolated islet grafts, both approaches remain experimental. Islet grafts might gain, over time, increasing credibility and might eventually provide an easier alternative in terms of grafting procedures and patient management, as compared with the more "traumatizing" whole-pancreas transplantation. Also, using islet, re-transplantation is possible. But it is not known whether re-transplantation of islet could be suitable for those patients who lost grafted islet function. The aim of the present study was to investigate the benefits of re-transplantation of islet in previously simultaneous islets-kidney transplant (SIK) patient who have lost graft function. Methods : The recipient was a 32 year old male. First islet transplantation was underwent at December 25, 1999. However, the grafted islets lost function after 70 days. So we performed re-transplantation of islets. The isolation of islet was conducted sterilely on a laminarflow hood and isolated by a modified Recordimethod. The islet was injected slowly into the liver via a cannular placed in the potalvein for 20 minutes. Results : Transplanted islets were 90,000 IEq at first islet transplantation, 370,000 IEq at second islet transplantation. The insulin requirement was reduced from 75-85 to 35-40 U/day, the basal C-peptide level was 1.5 ng/mL at 7 days posttransplant Unfortunately, the grafted islets lost function after 70 days. After second transplantation, the insulin requirement was reduced to 26 U/day. Conclusions : Despite the continuous need for exogenous insulin therapy, islet transplantation can prevent wide glucose fluctuations, thus resulting in normalization of glycemic control and improvement in HbAlc, and also, show that islets can be successfully and safely re-transplanted intraportally in patients who have lost previously grafted islet function (J Kor Diabetes Asso 457~466, 2000).

Interspecies hybrids of Panax ginseng Meyer new line 0837 and Panax quinquefolius generated superior F1 hybrids with greater biomass and ginsenoside contents

Kwang-Hoon Seo,Padmanaban Mohanan,Dong-Uk Yang,Yu Jin Kim,Woo-Saeng Kwon,DEOK-CHUN YANG 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4

Panax ginseng and Panax quinquefolius are important herbs with similar benefi ts and medicinal properties. Both possess ginsenosides, the pharmacologically active components, abundantly in their roots. In an attempt to establish ginseng cultivars with improved agronomical characters and higher ginsenoside content, reciprocal interspecifi c hybrids were generated by artifi cial pollination of emasculated fl owers. Three elite cultivars of P. ginseng , types Jakyung, Chungkyung, and Hwangsook, and two new lines, 0837 and 0702, were used to assess interspecifi c hybrid potential. Among F 1 reciprocal hybrids generated using line 0837 as either the maternal or paternal parent showed excellent agronomical traits and hybrid vigor. They showed strong root phenotypes with many lateral and fi ne roots resulting in 10 to 20% higher ginsenoside content compared to the parental lines. Among the major ginsenosides, pharmacologically active Re, Rb1, Rb2, and F1 were enriched. They accounted for 70% of the protopanaxadiol ginsenosides in 0837/ Pq and Pq /0837 crosses, displaying strong maternal dominance. Thus, based on our results, we conclude that P. ginseng line 0837 is superior compared to already existing lines for performing interspecifi c hybridization with promising outcomes in root quality and ginsenoside content.

Interspecies Hybrids of Panax ginseng Meyer New Line 0837 and Panax quinquefolius Generated Superior F1 Hybrids with Pharmacologically Enriched Ginsenosides

Kwang Hoon Seo(서광훈),Padmanaban Mohanan,Dong Uk Yang(양동욱),Yu Jin Kim(김유진),Woo Saeng Kwon(권우생),Jong Chan Ahn(안종찬),Jin Kyu Park(박진규),Deok Chun Yang(양덕춘) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.2

Characterization of Culture and Media Conditions for in vitro Propagation of Hippophae rhamnoides

Kwang Hoon Seo(서광훈),Padmanaban Mohanan(파드마나반 모하난),Sun Ju Park(박선주),Hee Ae Kim(김희애),Do Hyun Kim(김도현),Seul Ki Choi(최슬기),Deok Chun Yang(양덕춘) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.1