셀레콕시브 및 그 합성유도체들의 항암활성 스크리닝

박정란,강진형,구효정,노지영,류형철,박상욱,고동현,조일환,이주영,황다니엘,김인경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Selective COX (cyclooxygenase)-2 inhibitors including celecoxib have been shown to induce apoptosis and cell cycle changes in various tumor cells. New inhibitors are recently being developed as chemomodulating agents. We evaluated celecoxib and screened 150 synthetic compounds for anti-proliferative activities in vitro. Effects of celecoxib on COX activity, cell growth, cell cycle distribution, and apoptosis induction were determined in A549 COX-2 overexpressing human non-small cell lung cancer (NSCLC) cells. The COX inhibition of celecoxib increased with concentration up to 82% at 1μM after 24 hr exposure. Forty μM and 50μM of celecoxib induce G_1 arrest, and TUNEL-positive apoptotic cells, respectively. Among 150 compounds, several compounds were selected for having greater COX-2 inhibitory activity and higher selectivity than celecoxib with growth inhibitory activity. Celecoxib showed concentration-dependent COX inhibitory activity, and ability to induce cell cycle arrest and apoptosis in human NSCLC cells in vitro. Among synthetic analogues screened, several compounds showed promising in vitro activity as COX-2 inhibitory anticancer agents, which warrant further evaluation in vitro and in vivo.

Circuit Weight Training이 중년남성의 체격 및 체력에 미치는 영향

김연,김정주,고인태 경희대학교 체육과학연구소 1996 體育學論文集 Vol.24 No.-

The purpose of this study was to investigate the effect of circuit weight training on the physique, physical fitness. 20 middle ages man at B leisure town were selected as subject and then divided into two group; The forties group with 10 persons, fifties group with 10 persons. circuit weight training was three times a week for the period 8 weeks. To evaluate the charge of physique, skinfolds, muscular strength each group was measured. The pre test was enforced before taking the experiment, and the post test was operated after taking the experiment 8 weeks later. The results were as follows; 1.Significant change was increased in chest girth of forties group 2.54%(p〈.05), non significant change was fifties group 0.26%, significant change increased in the thigh girth of forties group 2.50%(p〈.001), fifties group 4.43(p〈.01), and non significant change was decreased in the waist girth of forties group 0.54, significant change was fifties group 1.24%(p〈.05) by after 8 weeks. 2.Significant change was decreased in triceps of forties group 16.81 (p〈.01), fifties group 18.10%(p〈.01), non significant change was in the abdomen of forties group 2.89%, significant change was in fifties group 5.02%(p〈.01), subscapular in the forties group 12.50%(p〈.01), fifties group 19.25%(p〈.01) after 8 weeks, and total thickness of forties group 13.93(p〈.01), fifties group 12.80%(p〈.01), significant change was increased in the body density of forties group 0.51%(p〈.05), fifties group 2.31%(p〈.01), LBM of forties group 2.86%(p〈.05), fifties group 0.2.31%(p〈.01), significant change was decreased in %Fat of forties group 11.91%(p〈.01), fifties group 8.60%, Fat of forties group 12.93%(p〈.01), fifties group 9.37%(p〈.01) by after 8 weeks. 3, Significant change was increased in the grip strength of forties group 8.64%(p〈.05), fifties group 6.24%(p〈.01), back strength of forties group 12.65%(p〈.01), fifties group 10.63%(p〈.01), trunk flexion of forties group 20.18%(p〈.001), fifties group 22.55%(p〈.01), non significant change was increased in the trunk extension of forties group 5.56%, fifties group 4.43% by after 8 weeks. In conclusion circuit weight training is effective as the method of all-round development physique, physical fitness.

두피 전체의 발모광을 보이는 정신분열병 1례

고정인,신성훈,이상열 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.1

Trichotillomania is characterized by recurrent pulling of one's hair, resulting in significant hair loss and involves many hours each day of pulling hair or thinking about pulling hair. Patients pulled hair from a variety of sites, including scalp, eyelashes, eyebrows, pubic region, face, and body, but the highest incidence and severity involved scalp hair. This symptom arises as a part of symptom complexes in a variety of psychiatric disorders such as obsessive compulsive disorder, depression, mental retardation, eating disorder and schizophrenic disorder. The authors experienced a case of trichotillomania in a 42 year old schizophrenic patient whose total hair in scalp was pull out. Several literatures were reviewed and psychiatric aspect of trichotillomania were discussed. For the treatment of this patient, haloperidol and supportive psychotherapy had been tried and been proven effective.

An unusual abscess formation in the masticator space after acupressure massage: a case report

In-Chan Ko,Kyu-Ho Yoon,Kwan-Soo Park,Jeong-Kwon Cheong,Jung-Ho Bae,Kwon-Woo Lee,Young-Jai Chin 대한구강악안면외과학회 2015 대한구강악안면외과학회지 Vol.41 No.1

Clinical features of masticator-space abscess (MSA) are very similar to those of parotitis or temporomandibular disorder (TMD), making early differ- ential diagnosis difficult. Local causes of MSA include nerve block anesthesia, infection after tooth extraction, and trauma to the temporomandibular joint (TMJ); the systemic cause is immunodeficiency. Odontogenic causes account for most etiologies, but there are also unusual causes of MSA. A 66-year-old male patient visited the emergency room (ER) presenting with left-side TMJ pain three days after receiving an acupressure massage. He was tentatively diagnosed with conventional post-trauma TMD and discharged with medication. However, the patient returned to the ER with in- creased pain. At this time, his TMD diagnosis was confirmed. He made a third visit to the ER during which facial computed tomographic (CT) images were taken. CT readings identified an abscess or hematoma in the left masticator space. After hospitalizing the patient, needle aspiration confirmed pus in the infratemporal and temporal fossa. Antibiotics were administered, and the abscess was drained through an incision made by the attending physician. The patient’s symptoms decreased, and he was discharged.

두피 전체의 발모광을 보이는 정신분열병 1례

고정인,신성훈,이상열 圓光大學校 醫科大學 神經精神科學敎室 1998 圓光精神醫學 Vol.14 No.1

Trichotillomania is characterized by recurrent pulling of one's hair, resulting in significant hair loss and involves many hours each day of pulling hair or thinking about pulling hair. Patients pulled hair from a variety of sites, including scalp, eyelashes, eyebrows, pubic region, face, and body, but the highest incidence and severity involved scalp hair. This symptom asries as a part of symptom complexes in a variety of psychiatric disorders such as obsessive compulsive disorder, depression, mental retardation, eating disorder and schizophrenic disorder. The authors experienced a case of trichotillomania in a 42 year old schizophrenic patient whose total hair in scalp was pull out. Several literatures were reviewed and psychiatric aspect of trichotillomania were discussed. For the treatment of this patient, haloperidol and supportive psychotherapy had been tried and been proven effective.

국내산 과일 및 채소 추출물을 이용한 체중 조절 관련 기능성 식품소재 탐색

김인혜;김종배;조강진;황유진;황경아;고정숙;엄애선 한양대학교 2011 韓國 生活 科學 硏究 Vol.31 No.1

Obesity can be defined as a disorder of lipid metabolism and the control of this process is regulated by enzymes involved in lipid metabolic pathway. Obesity is also characterized increases in the number and size of adipocyte differentiated from fibroblastic pre-adipocytes in adipose tissue. The aim of this study was to screen ethanol extracts of 21 fruits and vegetables cultivated in Republic of Korea for identifying natural sources of lipase inhibitor and to investigate the effects of several extracts resulting in suppression of triglyceride (TG) accumulation in 3T3-L1 cells. Among the tested extracts, Diospyros kaki L. showed the highest contents of total sugar and the content of total phenolic compounds was detected 17 species while there was not detected in Ixeris dentate root and Allium fistulosum C. etc. We were screened 26 extracts of fruits and vegetables for pancreatic lipase inhibitory activity compared with orlistat. The strongest lipase inhibitory activities were shown by extract of Allium sativum L. stem, Allium sativum L. bulb, Apteryx australis, Diospyros kaki L. Malus Pumila, Perilla frutescens and Prunus mume regardless of concentrations treated. Eight extracts including Brassica juncea var. integrilolia had no effect on lipase inhibition. During the differentiation of 3T3-L1 preadipocyte to mature adipocyte, the cells were treated with carrier or 7 extracts(1, 5 and 10 ㎍/ml), which was shown to highest lipase inhibitory effects. We were quantified lipid contents secreted mature adipocytes and stained with Oil Red O. All cells treated with the Apteryx australis and Prunus mume significantly decreased TG contents than the control. These findings suggested that the screened fruit and vegetable could be a promising source as the effective and safe lipase inhibitors for isolating bioactive ingredients and developing functional foods.

기분장애 환자의 스트레스와 정서반응 : 우울, 불안, 분노 반응

박민철,오상우,류미경,피상순,고정인 대한생물치료정신의학회 1999 생물치료정신의학 Vol.5 No.2

Objectives : This study was investigated to demonstrate the relationship between stress and emotional responses of depression, anxiety, and anger in patients with mood disorder.Methods : Fifty patients with major depressive disorder diagnosed by DSM-IV were compared to those with bipolar manic disorder. All subjects were evaluated for perceived stress, Beck depression inventory, dysfunctional attitude, state-trait anxiety, state-trait anger and anger expression. The data were analyzed by t-test, correlation, and multiple regression analysis.Results : The results were as follows.1. Depressive group had significantly higher score of perceived stressful life events, depression, state anxiety and trait anxiety than manic group.2. Perceived stress in depressive group was positively correlated with depression, anxiety, state and trait anger, anger expression and sex, however that in manic group was positively correlated with state anger and anger expression.3. In depressive group, trait anxiety, trait anger, age, and dysfunctional attitude predicted 70.6% of variance on depression, perceived stress predicted only 1.2%. Trait anxiety predicted 67.6% for state anxiety, and trait anger and trait anxiety predicted 41.5% for state anger, trait anger and sex predicted 34.0% for anger expression.4. In manic group, education and trait anger predicted 28.7% of variance of depression, and trait anger predicted 38.3% for state anxiety. Trait anger and education predicted 29.4% for state anger, and state anger and sex, state anxiety predicted 36.3% for anger expression.Conclusion : The results suggest that emotional responses to perceived stress in depressive group are related to depression, anxiety, and anger but those in manic group are related to anger.

운동부하테스트에 따른 정상혈압자의 혈압상승도가 고혈압 발병요인에 미치는 영향

권은영,김정주,고인태 경희대학교 체육대학 한국체육과학연구소 2003 體育學論文集 Vol.31 No.-

It is well established that the elevation of systolic blood pressure during exercise is the risk factor for cardiovascular diseases. The objective of this study is to examine whether the elevation of systolic blood pressure during exercise is correlated with the pathogenesis of hypotension To fulfill this objective, this study assigned 31(46.2±7.0 yr) middle-aged women to low group(n=17) and high group(n=14) according to the criteria for the classification of hypertension(JNC-VI, 1997). Following this, this study analyzed cardiovascular factors(SBP, DBP, HR, VO₂, RER) and blood components(LDLC, HDLC, TC, TG, HDLC/LDLC). The results of the present study were as follows: (1) SBP was significantly increased at resting state, at 4 minutes, at 6 minutes and at endpoint(P<.05), (2) There was significant difference between SBPmax and SBPrest(P<.05), (3) DBP was significantly increased solely at resting state SBPmax (P<.05), (4) RER was significantly increased at 10 minutes. In addition, a statistical analysis revealed that SBPmax-SBPrest was significantly correlated with age(r=0.336), SBPmax(r=0.827) and DBPmax(r=0.335). In conclusion this study indicates that the monitoring of such cardiovascular indicators as SBP, DBP, HR, VO₂ and RER is essential in health promotion in patients with obesity or normal healthy people as weil as patients with hypertension.

Disc 적층법에 의한 ZrO_2/Ni계 경사기능재료의 제조에 관한 연구

尹正模,高仁用,朴豊玉 全北大學校 1996 論文集 Vol.42 No.-

Doctor blade is the method for getting the FGM by the steps of making slurry and stacking, drying, de-greasing, pressing sintering method after adding binder, deflocculant, plasticizer as the additives and ethyl alcohol as the solvent to the powder material. In this experiment, a study on the development of manufacture of FGM, we tried to magnify the size of FGM on the basis of spray stacking method, sintering method and powder stacking method.

DNA Conformation Probe로서의 Anionic Potential Reporter 분자의 구조 연구 : Ⅰ. Nalidixic Acid의 공간군 결정 Ⅰ. The Space Group Determination of Nalidixic Acid

오인근,고동성,이천배,서정인,서일환 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.1

The space group of nalidixic acid(1-ethyl-1, 4-dihydro-7-methyl-4-oxo-1, 8-naphthyridine-3-carboxylic acid, C_12H_12N_2O_3), a clinically important antibiotics, has been determined by single crystal X-ray photographic data collected three-dimensionally using Weissenberg method with Cu Kα radiation (λ=1.5418 Å), as the structural data is required and important for the elucidation of the molecular mechanism of the inhibitory action of the antibiotics on DNA synthesis. The space group is P2_1/c with four molecules in the unit cell of dimensions, a=8.98±0.15, b=13.30±0.03, c=9.50±0.16Å, and β=99.5±0.1°.