Effect of Developmental Lead Exposure on the Expression of Hippocampal NMDA Receptor Subunit mRNA

Kim, Tae-Wan,Chung, In-Sung,Bae, Jae-Hoon,Shin, Dong-Hoon,Lee, Mi-Young,Kim, Joon-Sik 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

목적: in vivo 및 vitro에서 해마 신경세포의 발생단계별 NMDA 수용체 아단위 mRNA 발현에 대한 연 폭로 영향을 알아보고자 하였다. 방법: 흰쥐 해마 신경세포의 발생단계별 NMDA 수용체 NR2A, NR2B 아단위 mRNA 발현에 대한 연의 영향은 정상군과 연 폭로군의 출생 후 7일, 14일, 22일 흰쥐의 해마에서 in situ hybridization으로 mRNA 발현 정도를 densitometer로 측정하여 비교하였고, 연과 NMDA 의 세포독성은 해마 신경세포 일차배양 후 도립현미경을 이용한 형태학적인 관찰과 LDH 활성도를 이용하여 측정하였다. 결과: 연 과 NMDA 에 의한 세포독성에 대한 in vitro 실험에서 형태학적 소견과 LDH 활성도에서 해마 미성숙 신경세포와 성숙 신경세포사이의 차이가 있었으므로, 연과 NMDA 독성효과는 해마 신경세포의 발달 단계에 따라 차이가 있다. 정상군의 해마에서의 NR2A mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나, NR2B mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나 NR2B mRNA 발현은 연령의 증가에 따른 변화가 없었다. 연 폭로에 희한 NR2A mRNA 발현은 유의하게 감소하였으나(p<0.05), NR2B mRNA 발현은 변화가 나타나지 않았다. 만성적 연 폭로는 NR2A를 포함하는 NMDA 수용체를 감소시킬 수 있음을 알 수 있다. 결론: 연은 해마신경세포의 발생단계에서 NMDA 수용체 아단위 특히 NR2A mRNA 발현의 변화를 야기하여 시냅스 신호 전달에 영향을 나타냄을 알 수 있었다. Hippocampus

Associations of serotonergic genes with poststroke emotional incontinence

Kim, Jae‐,Min,Stewart, Robert,Kang, Hee‐,Ju,Bae, Kyung‐,Yeol,Kim, Sung‐,Wan,Shin, Il‐,Seon,Kim, Joon‐,Tae,Park, Man‐,Seok,Cho, Ki‐,Hyun,Yoon, Jin‐ John Wiley Sons, Ltd 2012 INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY Vol.27 No.8

<P><B>Objectives</B></P><P>Poststroke emotional incontinence (PSEI) has been associated with serotonergic dysfunction. Polymorphisms of serotonin transporter (5‐HTT) and serotonin 2a receptor (5‐HTR2a) genes may regulate serotonergic signaling at brain synapses, and this study was to investigate associations with PSEI in an East Asian population.</P><P><B>Methods</B></P><P>In 276 stroke cases, PSEI was diagnosed by Kim's criteria. Covariates included age, gender, education, history of depression or stroke, current depression, and stroke severity and location. Genotypes were ascertained for 5‐HTT gene‐linked promoter region (5‐HTTLPR), serotonin transporter intron 2 variable number tandem repeat, 5‐HTR2a 1438A/G, and 5‐HTR2a 102 T/C. Associations with PSEI were estimated by using logistic regression models, and gene–gene interactions were investigated by using the generalized multifactor dimensionality reduction method.</P><P><B>Results</B></P><P>PSEI was present in 37 (13.4%) patients. The 5‐HTT gene‐linked promoter region <I>s</I>/<I>s</I> genotype was independently associated with PSEI. No associations with STin2 VNTR and 5‐HTR2a genes were found, and no significant gene–gene interactions were identified.</P><P><B>Conclusions</B></P><P>Stroke patients with 5‐HTTLPR <I>s</I> allele had higher susceptibility to PSEI, which underlines the potential role of serotonergic pathways in its etiology. Copyright © 2011 John Wiley & Sons, Ltd.</P>

The level of urinary aflatoxin M1 in Korean adults

Yong-Dae Kim1, Hyojin Kwon, Sun-In Moon, Sang-Yong Eom, Jung-Duk Park, Byung-Sun Choi, Seok-Joon Sohn, Young-Seoub Hong, Ho Kim, Ho-Jang Kwon, Ji-Ae Lim, Hae-Jung Yoon, Gwang-Jin Kim, Heon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Competitive ELISA methods were used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 Korean adults. Subjects were selected by random sampling in all areas of Korea, except Cheju-do. The recovery rate of AFM1 using this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/g creatinine). The level of AFM1 in males was statistically higher, compared with female subjects. However, the levels of AFM1 did not differ according to age. Subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas subjects in Kyeongnam-do showed the lowest concentration. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to approximately 0.1 microgram/day in Korean adults.

단일공법 복강경하 질식자궁절제술 110예의 고찰 및 다공법과의 비교

박병준 ( Byung Joon Park ),김용욱 ( Yong Wook Kim ),노덕영 ( Duck Yeong Ro ),김태응 ( Tae Eung Kim ),류기성 ( Ki Sung Ryu ),김장흡 ( Jang Heup Kim ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.7

목적: 통상적인 복강경기구만을 사용한 단일공법 복강경하 질식자궁절제술을 다공법 복강경하 질식자궁절제술과 비교하여 안전성 및 유용성을 평가하고자 하였다. 연구 방법: 2007년 4월부터 2009년 11월까지 인천성모병원에서 자궁근종 또는 자궁샘근증의 질환으로 단일공법 복강경하 질식 자궁절제술을 시행받은 환자 110명과 다공법 복강경하 질식자궁절제술을 시행받은 환자 110명, 총 220명의 환자를 대상으로 하였다. 우리는 모든 수술에서 통상적으로 사용되는 구부러지지 않는 일직선의 복강경기구를 사용하였다. 또한 단일공법 및 다공법 복강경하 질식자궁절제술 둘 다에서 수술자 (Kim, YW)의 이름을 따서 명명한 새로운 질단부 봉합 방법인 김씨 질구개 현수법 (Kim`s Vaginal Vault Suspension Method)을 사용하였다. 결과: 환자 나이, 수술 시간, 자궁의 무게, 혈색소 수치의 변화, 수혈 빈도, 수술 후 발열에서 두 군 간에 유의한 차이를 보이지 않았다. 환자들의 평균 나이는 46.7±7.0세(단일공법)와 45.5±6.3세(다공법)이었다. 평균 수술 시간은 87.2±21.0분(단일공법)과 83.3±20.3분(다공법), 평균 자궁의 무게는 261.4±139.7 g(단일공법)과 257.8±132.9 g(다공법), 평균 혈색소 수치의 변화는 1.1±0.7 g/dL(단일공법)와 1.2±0.6 g/dL(다공법)이었다. 두 군 모두에서 수술 중 장 또는 요로의 손상은 없었다. 두 군 모두에서 각각 한 건의 개복수술로의 전환이 있었다. 단일공법을 시행한 군에서 입원 기간이 유의하게 짧았다(2.6±0.6일 [단일공법], 3.3±0.7일 [다공법], P<0.05). 결론: 통상적으로 사용하는 구부러지지 않는 일직선의 복강경기구를 이용한 단일공법 복강경하 질식자궁절제술은 다공법 복강경하 질식자궁절제술에 대한 안전하고 유용한 대체 수술이 될 수 있다. Objective: To evaluate the safety and feasibility of single-port access laparoscopically assisted vaginal hysterectomy (SPA-LAVH) using conventional laparoscopic instruments compared to multi-port access laparoscopically assisted vaginal hysterectomy (MPA-LAVH). Methods: We reviewed the medical records of 220 patients with uterine leiomyoma or adenomyosis who underwent 110 SPA-LAVH and 110 MPA-LAVH in Incheon St. Mary`s Hospital between April 2007 and November 2009. We performed SPA-LAVH with conventional rigid straight laparoscopic instruments in all cases. We also performed a new vaginal cuff closure method, Kim`s Vaginal Vault Suspension Method, named after the operator (Kim, YW) in both SPA-LAVH and MPA-LAVH. Results: There was no significant difference in patients` age, operating time, uterine weight, hemoglobin change, frequency of blood transfusion, and incidence of postoperative fever between the two groups. The patients` mean age was 46.1±7.0 years (SPA-LAVH) and 45.5±6.3 years (MPA-LAVH). The mean operating time was 87.2±21.0 minutes (SPA-LAVH) and 83.3±20.3 minutes (MPA-LAVH). The mean uterine weight was 261.4±139.7 g (SPA-LAVH) and 257.8±132.9 g (MPA-LAVH). The mean hemoglobin change was 1.1±0.7 g/dL (SPA-LAVH) and 1.2±0.6 g/dL (MPA-LAVH). Neither bowel injury nor urinary tract injury occurred during the operation in the two groups. One of the SPA-LAVH and one of the MPA-LAVH cases were converted to abdominal total hysterectomy. The mean hospital stay time was shorter with SPA-LAVH (2.6±0.6 days [SPA-LAVH] and 3.3±0.7 days [MPA-LAVH], P<0.05). Conclusion: SPA-LAVH using conventional rigid straight laparoscopic instruments can be offered as a safe and feasible alternative to MPA-LAVH.

K-식(式) 침전관(沈澱管)에 의한 간질충란(肝蛭蟲卵) 간이검사법(簡易檢査法)에 관한 연구(硏究)

김교준,김상근,허민도,Kim, Kyo Joon,Kim, Sang Keun,Hu, Min Do 충남대학교 농업과학연구소 1982 Korean Journal of Agricultural Science Vol.9 No.2

피내반응법(皮內反應法)에 의(依)해 간질(肝蛭) 양성반응(陽性反應)으로 판정(判定)된 유우(乳牛)를 대상(對像)으로 분편검사(糞便檢査)를 실시(實施)하여 충란(蟲卵)을 확인(確認)한 후(後), 현재(現在) 사용(使用)되고 있는 간질(肝蛭) 충란검사법(蟲卵儉査法)들의 단점(短點)을 개선(改善)하기 위하여 잔재물(殘滓物)을 성력적(省力的)으로 제거(除去)하는 사(篩)의 조합법(組合法)과 K-식(式) 침전관(沈澱管)을 고안(考案)하여, 인위적(人爲的)으로 분즙(糞汁)에 충란(蟲卵)을 혼입(混入)하여 K-식(式) 침전관법(沈澱管法)에 따라 검사(檢査)한 결과(結果) 현재(現在)까지 보고(報告)된 타방법(他方法)들에 비(比)하여 조작(操作)이 간편(簡便)하고 검출율(檢出率)도 높아 이상적(理想的)인 방법(方法)으로 사료(思料)되었기에 그 개요(槪要)와 결과(結果)를 보고(報告)하는 바이다. 1. 간질충란(肝蛭蟲卵)을 여과(慮過)하고 잔재물(殘滓物)을 제거(除去)하기 사(篩)의 조합(組合)을 3단계(段階)(100mesh, 150mesh, 250mesh)로 조합(組合)하여 성력적(省力的)인 방법(方法)으로 개선(改善)하였다. 2. 3종(種)의 침전관중(沈澱管中) 제(第)III형(型)의 침전관(沈澱管)에 의(依)한 간질충란(肝蛭蟲卵) 회수율(回收率)이 가장 높았으므로 제(第)III형(型)을 K-식(式) 침전관(沈澱管)으로 지칭(指稱) 채택(採擇)하였다. 3. K-식(式) 침전관법(沈澱管法)에서 A-II, A-III, B-III, C-III형(型)의 1구(回) 경검성적(鏡檢成績)은 99~100%였으며 A-I, B-II, C-I 및 C-II형(型)은 71~91%였다. 4. A형(型)($5m{\ell}$, 5-10침전(沈澱)), B형(型)($10m{\ell}$, 10-10침전(沈澱)) 및 C형(型)($15m{\ell}$, 15-15침전(沈澱))은 각기(各其) 특징(特徵)을 가진 방법(方法)들로서 판정시간(判定時間)을 단축(短縮)하는데는 A형(型)이 가장 유용(有用)한 방법(方法)이었다. 5. K-식(式) 침전관법(沈澱管法)은 다른 검출법(檢出法)에 비(比)하여 가장 검출율(檢出率)이 높은 간편(簡便)한 방법(方法)이었으며 E. P. G가(價)도 동시(同時)에 얻을 수 있는 이상적(理想的)인 방법(方法)으로서 임상응용가치(臨床應用價値)가 매우 높은 것으로 사료(思料)되었다. These studies were carried out to investigate of detection of Fasciola egg from positive cattle faces in the intradermal reaction method by laborsaving composition of sieves and Kim's sedimental tube method. The results obtained are summerized as follows: 1. Detection method of Fasciola eggs was improved by 3 meshes(100mesh, 150mesh, 250mesh) for filtration of fecal fluid contained Fasciola eggs. 2. III type of tube in the 3 kinds of sedimental tube had the highest recovery rate of Fasciola egg. Thus, this type of tube was selected and called as Kim's sedimental tube. 3. A-II, A-III, B-III and C-III type by Kim's sedimental tube method had the recovery rates of eggs in the 1st dropping as ranged 99 to 100% and A-I, B-II, C-I and C-II as 71 to 91%. 4. All types as themselves contained the special aspects. In the these type was useful to determine the results in the urgent cases add to shorten the time of the diagnostic procedures. 5. Kim's sedimental tube method high recovery rate and convenient procedures as compared with other detection methods reported. In addition, through this method can also obatained E. P. C. value. Therefore, it is desirable that Kim's sedimental tube method be recommended by clinicians.

K-식(式) 침전관(沈澱管)에 의한 간질충란(肝蛭蟲卵) 간이검사법(簡易檢査法)

김교준,김상근,허민도,Kim, Kyo-Joon,Kim, Sang-Keun,Hu, Min-Do 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.1

The studies carried out to investigate the detection method of Fasciola eggs from positive cattle feces in the intradermal reaction method with laborsaving composition of sieves and Kim's sedimental tube method. The results obtained are summarized as follows: 1. The detection method of Fasciola eggs was improved by 3 meshes (100mesh, 150mesh, 250mesh) for filteration of fecal fluid contained Fasciola eggs. 2. Third type of tube in the 3 kinds of sedimental tube had the highest recovers rate of Fasciola eggs. Thus, this type of tube was selected and called as Kim's sedimental tube. 3. A-II, A-III, B-III and C-III type by Kim's sedimental tube method had the recovery rates of eggs in the 1st drop as ranged 99 to 100% and, A-I, B-II, C-II as 71 to 91%. 4. These Type of tubes were useful to detect Fasciola eggs and to shorten the time of the diagnostic procedures. 5. The Kim's sedimental tube method showed high recovery rates and convenient procedures as compared with other detection methods reported. In addition, the EPG value can also be obtained by this method. Therefore, it is desirable that the Kim's sedimental tube method could be recommended to clinicians.

Identification of a novel peptide that selectively binds to Kidney Injury Molecule-1 (KIM-1) by phage displayed peptide library

Tae-Jun Kwon,Joon-Suk Park,Sang-Hyun Kim,Enamul Haque,Byung-Heon Lee,Sang Eun Kim,Choong-Yong Kim,Sang Kyoon Kim 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

초음파 C-scan을 이용한 터빈 블레이드 열차폐코팅의 등온열화에 의한 박리 평가 기법

이호걸(Ho-Girl Lee),김학준(Hak-Joon Kim),송성진(Sung-Jin Song),석창성(Chang-Sung Seok) 한국비파괴검사학회 2016 한국비파괴검사학회지 Vol.36 No.5

가스터빈 블레이드는 고온 고압의 환경 아래 장시간 가동하기 위하여 초합금 모재에 세라믹 코팅으로 이루어진 열차폐코팅(thermal barrier coating, TBC)은 필수요소이다. 하지만 TBC 또한 가스터빈 가동 중 일정 열화온도 및 가동시간에서 top coat의 박리현상이 일어난다. TBC의 박리는 블레이드의 손상과 직결되므로 가스터빈의 안정적인 가동을 위해서 TBC의 박리 평가가 선행되어야 한다. 기존 비파괴평가 기법 연구는 산화알루미늄층(thermally grown oxide, TGO)의 생성 유무 또는 완전 박리의 정성적 평가가 이루어져 왔다. 본 연구에서는 TBC 박리를 정량적으로 평가하기 위해 초음파검사의 C-scan기법을 이용한 TBC의 부분박리손상 map을 구현하였다. 시편들은 1,100˚C로 등온열화하여 각각 열화시간을 변화시킨 시편들을 사용하였다. 단일 탐촉자를 이용한 펄스-에코법으로 C-scan을 수행하였고 TBC 내 부분박리를 검출하기 위해 초음파를 수침법으로 시편에 수직탐상하였다. 그리고 Rogers-Van Buren과 Kim의 이론 반사식을 이용하여 부분박리영역 지름이 1 mm부터 6 mm까지 부분박리지수를 도출했다. 이를 적용하여 각 부분박리지수에 따른 부분박리 손상 map을 영상화하였다. TBC는 열화시간이 증가할수록 부분박리지수에 관계없이 부분박리영역이 모두 증가함을 확인할 수 있었다. 또한 단일 시편 내에서 부분박리지수가 증가할수록 부분박리영역이 감소하는 것을 확인하였다. 부분박리손상 map의 부분박리영역에 따른 분포를 이용하여 TBC의 완전박리 기준과 잔여 수명을 또한 도출할 수 있었다. Thermal barrier coating (TBC) is an essential element consisting of a super-alloy base and ceramic coating designed to achieve long operational time under a high temperature and pressure environment. However, the top coat of TBC can be delaminated at certain temperatures with long operation time. As the delamination of TBC is directly related to the blade damage, the coupling status of the TBC should be assured for reliable operation. Conventional studies of nondestructive evaluation have been made for detecting generation of thermally grown oxide (TGO) or qualitatively evaluating delamination in TBC. In this study, the ultrasonic C-scan method was developed to obtain the damage map inside TBC by estimating the delamination in a quantitative way. All specimens were isothermally degraded at 1,100˚C with different time, having different partial delamination area. To detect partial delamination in TBC, the C-scan was performed by a single transducer using pulse-echo method with normal incidence. Partial delamination coefficients of 1 mm to 6 mm were derived by the proportion of the surface reflection signal and flaw signal which were theoretical signals using Rogers-Van Buren and Kim"s equations. Using the partial delamination coefficients, the partial delamination maps were obtained. Regardless of the partial delamination coefficient, partial delamination area was increased when degradation time was increased in TBC. In addition, a decrease in partial delamination area in each TBC specimen was observed when the partial delamination coefficient was increased. From the portion of the partial delamination maps, the criterion for delamination was derived.

Chronic Hepatitis B Infection Is Significantly Associated with Chronic Kidney Disease: a Population-based, Matched Case-control Study

Kim, Sung-Eun,Jang, Eun Sun,Ki, Moran,Gwak, Geum-Youn,Kim, Kyung-Ah,Kim, Gi-Ae,Kim, Do Young,Kim, Dong Joon,Kim, Man Woo,Kim, Yun Soo,Kim, Young Seok,Kim, In Hee,Kim, Chang Wook,Kim, Ho Dong,Kim, Hyun The Korean Academy of Medical Sciences 2018 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.33 No.42

<P><B>Background</B></P><P>Hepatitis B virus (HBV) infection leads to hepatic and extrahepatic manifestations including chronic kidney disease (CKD). However, the association between HBV and CKD is not clear. This study investigated the association between chronic HBV infection and CKD in a nationwide multicenter study.</P><P><B>Methods</B></P><P>A total of 265,086 subjects who underwent health-check examinations in 33 hospitals from January 2015 to December 2015 were enrolled. HBV surface antigen (HBsAg) positive cases (n = 10,048), and age- and gender-matched HBsAg negative controls (n = 40,192) were identified. CKD was defined as a glomerular filtration rate (GFR) < 60 mL/min/1.73 m<SUP>2</SUP> or proteinuria as at least grade 2+ of urine protein.</P><P><B>Results</B></P><P>HBsAg positive cases showed a significantly higher prevalence of GFR < 60 mL/min/1.73 m<SUP>2</SUP> (3.3%), and proteinuria (18.9%) than that of the controls (2.6%, <I>P</I> < 0.001, and 14.1%, <I>P</I> < 0.001, respectively). In the multivariate analysis, HBsAg positivity was an independent factor associated with GFR < 60 mL/min/1.73 m<SUP>2</SUP> along with age, blood levels of albumin, bilirubin, anemia, and hemoglobin A1c (HbA1c). Likewise, HBsAg positivity was an independent factor for proteinuria along with age, male, blood levels of bilirubin, protein, albumin, and HbA1c. A subgroup analysis showed that HBsAg positive men but not women had a significantly increased risk for GFR < 60 mL/min/1.73 m<SUP>2</SUP>.</P><P><B>Conclusion</B></P><P>Chronic HBV infection was significantly associated with a GFR < 60 mL/min/1.73 m<SUP>2</SUP> and proteinuria (≥ 2+). Therefore, clinical concern about CKD in chronic HBV infected patients, especially in male, is warranted.</P>

Hepatitis C Virus Infection Is Associated with Lower Levels of Serum Cholesterol, Triglyceride, and LDL-Cholesterol Level: A Population-Based, Matched Case-Control Study in Korea

( Sung Eun Kim ),( Eun Sun Jang ),( Moran Ki ),( Geum-Youn Gwak ),( Kyung-Ah Kim ),( Gi-Ae Kim ),( Do Young Kim ),( Dong Joon Kim ),( Man Woo Kim ),( Yun Soo Kim ),( Young Seok Kim ),( In Hee Kim ),( 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hepatitis C virus (HCV) entry and replication is associated with interruption of lipid metabolism. We aimed to investigate the association between anti-HCV positivity and serum lipid profiles in a nationwide, multicenter study. Methods: A total of 268,422 subjects who underwent health-check examination in nationwide 33 hospitals from Jan 2015 to Dec 2015 were enrolled. Data on the anti-HCV positivity, and biochemical laboratory results including serum levels of total cholesterol, triglyceride, LDL-cholesterol, and HDL-cholesterol were obtained and analyzed. Among the subjects, 1,360 anti-HCV antibody positive cases (0.6%), and 5,440 anti-HCV negative controls matched for age and sex to the cases were identified in same dataset. Results: Though body mass index (BMI) was not different between case and control group, the mean serum levels of total cholesterol (185 mg/dL), triglyceride (111.8 mg/dL), LDL cholesterol (117.4 mg/dL), and HDL cholesterol (54.5 mg/dL) were all significantly lower in anti-HCV positive group than in controls (192.5, 119.1, 125.1, and 55.8 mg/dL, respectively). By multivariable analyses using logistic regression, the independent factors associated with elevated serum total cholesterol (≥200 mg/dL) were age, male sex, anti-HCV positivity or HCV-RNA positivity. In addition, the independent factors associated with elevated serum triglyceride(≥150 mg/dL) were age, male sex, BMI (≥25kg/m2) HbA1C (≥5.5%), ALT (≥40IU/L), anti-HCV positivity or HCV-RNA positivity. Moreover, the independent factors associated with elevated LDL-cholesterol (≥130 mg/dL) were age, male sex, and anti-HCV positivity or HCV-RNA positivity. Conclusions: In this large population-based data, HCV infection independently associated with lower serum total cholesterol level, lower triglyceride level and lower LDL cholesterol level.