Genetic effect of <i>CCR3</i> and <i>IL5RA</i> gene polymorphisms on eosinophilia in asthmatic patients

Lee, June-Hyuk,Chang, Hun Soo,Kim, Ji Hyun,Park, Se-Min,Lee, Yong Mok,Uh, Soo Taek,Rhim, Taiyoun,Chung, Il Yup,Kim, Yong-Hoon,Park, Byung Lae,Park, Choon-Sik,Shin, Hyoung Doo Elsevier 2007 The journal of allergy and clinical immunology Vol.120 No.5

<P><B>Background</B></P><P>Eosinophilic infiltration and peripheral blood eosinophilia in asthma require the cooperation of eosinophil-specific cytokines and chemokines and their receptors.</P><P><B>Objective</B></P><P>We investigated the association of polymorphisms in <I>CCR3</I> and <I>IL5RA</I> with asthma susceptibility or peripheral blood eosinophilia and the effects of the polymorphisms on receptor expression.</P><P><B>Methods</B></P><P>Polymorphisms in <I>CCR3</I> and <I>IL5RA</I> were identified and genotyped in 576 asthmatic patients and 180 healthy control subjects. CCR3 and IL-5 receptor α (IL-5Rα) protein expression on eosinophils was measured by means of flow cytometry.</P><P><B>Results</B></P><P>Although polymorphisms in <I>CCR3</I> were not associated with asthma susceptibility, the <I>CCR3</I> haplotype <I>ht2</I> showed a negative gene dose effect on the eosinophil count (<I>P</I> = .003–.009). <I>IL5RA c.−5091G>A</I> was weakly associated with eosinophil count. The effects of <I>ht2</I> were greater when paired with <I>IL5RA c.−5091A</I> (<I>P</I> = .001–.002). CCR3 protein expression was higher on eosinophils of asthmatic patients without <I>ht2</I> than in those with <I>ht2</I>. Asthmatic patients with the <I>IL5RA c.−5091A</I> allele showed higher IL-5Rα expression than those who were homozygous for the G allele.</P><P><B>Conclusion</B></P><P>The genetic association between <I>CCR3</I> polymorphisms and the number of circulating eosinophils was revealed as a novel finding. These associations were more pronounced when the <I>CCR3</I> polymorphisms were paired with polymorphisms in <I>IL5RA</I>. The protein expression levels of CCR3 and IL-5Rα on peripheral blood eosinophils are associated with the polymorphisms on their own genes.</P><P><B>Clinical implications</B></P><P>The identification of single nucleotide polymorphisms and haplotypes of <I>CCR3</I> and <I>IL5RA</I> might be useful in developing markers for intermediate phenotypes of eosinophil number and in designing strategies to control diseases related to hypereosinophilia.</P>

관상동맥질환을 동반한 모야모야병 1례

김동기,이영태,김기향,김성만,김대경,김두일,김동수 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Moyamoya disease is an occlusive intracranial arteriopathy with an abnormal cerebral vascular collateral networks. Although this disease is known to be associated with stenosis of extracranial arteries, such as the renal artery, a case associated with stenosis of the coronary artery is extremely rare. We report a case of coronary artery occlusive disease affected by Moyamoya disease. The patient was a 38-year-old female experiencing intermittent headache and exer tional chest pain. Cerebral MRI showed complete obstruction of both internal carotid arteries and abnormal cerebrovascular systems which confirmed Moyamoya disease. Coronary angiography showed a significant stenosis of left anterior descending coronary artery ostium with TIMI grade I collateral blood flow from the normal right coronary artery. After coronary artery stenting and medical treatment for headache, her subjective symptoms were relieved. In Moyamoya patient, an evaluation of the extracranial (especially coronary artery) vascular system should be considered if there are some evidences of coronary artery disease.

급성심근경색후 발생한 심실중격결손 2례

김태희,이재호,김애란,허민영,문치숙,정수룡,김대경,김두일,김동수 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Ventricular septal defect remains an infrequent but devastating complication of acute MI. Approximately 1∼3% of cases of acute myocardial infarction are complicated by ventricular septal rupture in the prethrombolytic era and the incidence has decreased to 0.2% with reperfusion therapy. This mechanical complication usually occurs within the first 10 to 14 days when necrotic tissue is most abundant and the collateral coronary circulation is not well developed. This lesion is generally associated with complete coronary obstruction rather than severe stenosis. Rupture of the ventricular septum is a severe mechanical complication of acute myocardial infarction, usually resulting in death unless surgical repair is performed. The bad prognosis of this event within the first 2 weeks indicates the need for early surgical rapair. This complication is more frequent after the first acute myocardial infarction in the elderly and secondary to a transmural myocardial infarction. We report two cases of ventricular septal rupture after acute myocardial infarction.

CS 약침의 항염증 및 항산화 활성에 대한 연구

김학주 ( Hak Ju Kim ),김수경 ( Soo Kyung Kim ),최일환 ( Il Hwan Choi ),정철 ( Chul Jung ),여서원 ( Hsu Yuan Lu ),두경희 ( Kyeong Hee Doo ),김민경 ( Min Kyung Kim ),조승연 ( Seung Yeon Cho ),박정미 ( Jung Mi Park ),고창남 ( Chang 대한면역약침학회 2012 대한면역약침학회지 Vol.1 No.1

Objectives: CS has been used as effective pain reducing pharmacoacupunture in clinical fields, however its constituents and physiological activity have not been yet certified by experiment. This experimental research was to analyze the component of CS Yakchim and prove its anti-oxidant and anti-inflammatory effect. Methods: Content of protein, glucose, crude fat and crude ash was calculated. Anti-oxidant effect was measured using 1,1-diphenyl-2-picryl hydrazyl(DPPH) free radical scavenging activity. Anti-inflammatory effect was measured with COX-2 kit and RAW 264.7 macrophage. the concentration of TNF-α and IL-6 was measured by Enzyme-linked immuno-sorbent assay. Results: CS Yakchim is consist of 0.17% of protein, 0.32% of glucose, 95.19% of crude fat and 0.32% of crude ash. CS showed better anti-oxidant effect than its control sample. CS COX-2 inhibiting capacity was better than its Indomethacin control sample. Inflammatory cytokine production was reduced but not at transcription level. Conclusions: These results showed that CS Yakchim is consist protein, glucose, crude fat and crude ash. CS Yakchim anti-oxidant and anti-inflammatory effect.

Solar Cells: Amorphous Zinc Stannate (Zn₂SnO₄) Nanofibers Networks as Photoelectrodes for Organic Dye‐Sensitized Solar Cells (Adv. Funct. Mater. 25/2013)

Choi, Seung‐,Hoon,Hwang, Daesub,Kim, Dong‐,Young,Kervella, Yann,Maldivi, Pascale,Jang, Sung‐,Yeon,Demadrille, Renaud,Kim, Il‐,Doo WILEY‐VCH Verlag 2013 Advanced Functional Materials Vol.23 No.25

<P>Highly porous amorphous Zn<SUB>2</SUB>SnO<SUB>4</SUB> electrodes are prepared using electrospinning techniques and combined with organic or ruthenium dyes to fabricate dye‐sensitized solar cells. As reported by Sung‐Yeon Jang, Renaud Demadrille, Il‐Doo Kim, and co‐workers on page 3146, the devices based on 3‐μm‐thick electrodes and the organic dyes demonstrate significantly improved performances compared to those using the ruthenium complex. Using this approach, solar cells with power conversion efficiencies up to 3.7% are obtained. </P>

HPLC-ICP/MS 와 ICP/MS를 이용한 FAPAS 07187 쌀의 총비소, 무기비소, 카드뮴 측정

노현명 ( Hyun Myung Noh ),김지영 ( Ji Young Kim ),김원일 ( Won Il Kim ),( Anitha Kunhikrishnan ),김민지 ( Min Ji Kim12 ),김경진 ( Gyeong Jin Kim ),박기문 ( Ki Moon Park ),류지혁 ( Ji Hyock Yoo ),김두호 ( Doo Ho Kim ),이영자 ( Y 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

Metal (loid)s such as environmental contaminants affect food safety. Metal (loid)s exist trace extremely in agriculture products. Therefore it is important to analyze accurately about trace these metals. High resolution techniques and quality controls are needed to analyze these materials. In order to achieve quality results, standardized methods and techniques are required. So ISO(International Organization for Standardization) of laboratory proficiency testing is determination of laboratory testing performance by means of inter-laboratory comparison. The values have been derived as a consensus of a number of laboratories using a variety of methods. Proficiency tests were applied to analyze the metal(loid)s [inorganic arsenic (As), total As and cadmium (Cd)] in powdered rice FAPAS 07187 (Food Analysis Performance Assessment Scheme; February-April 2013). The rice powder sample was extracted with 1% HNO3 for inorganic As for microwave-assisted extraction using water at 95℃ for 90 min and their analysis by HPLC/ICPMS. Also, HNO3 for total As and Cd using microwave-assisted extraction using at 200℃ for 50 mins and analysis by ICP/MS. Validation check was performed for accuracy using CRM (NMIJ CRM 7503-a) for inorganic As, and CRM (IRMM-Rice CRM) for total arsenic and cadmium. The result were satisfactory. FAPAS 07187 the z-scores of inorganic As, total As and Cd in powdered rice were-1.8,-0.9 and 0.2(lzl<2, respectively).

Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

A soft biomolecule actuator based on a highly functionalized bacterial cellulose nano-fiber network with carboxylic acid groups

Wang, Fan,Jeon, Jin-Han,Park, Sukho,Kee, Chang-Doo,Kim, Seong-Jun,Oh, Il-Kwon The Royal Society of Chemistry 2016 SOFT MATTER Vol.12 No.1

<P>Upcoming human-related applications such as soft wearable electronics, flexible haptic systems, and active bio-medical devices will require bio-friendly actuating materials. Here, we report a soft biomolecule actuator based on carboxylated bacterial cellulose (CBC), ionic liquid (IL), and poly (3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) electrodes. Soft and biocompatible polymer-IL composites were prepared via doping of CBC with ILs. The highly conductive PEDOT: PSS layers were deposited on both sides of the CBC-IL membranes by a dip-coating technique to yield a sandwiched actuator system. Ionic conductivity and ionic exchange capacity of the CBC membrane can be increased up to 22.8 times and 1.5 times compared with pristine bacterial cellulose (BC), respectively, resulting in 8 times large bending deformation than the pure BC actuators with metallic electrodes in an open air environment. The developed CBC-IL actuators show significant progress in the development of biocompatible and soft actuating materials with quick response, low operating voltage and comparatively large bending deformation.</P>

일제에 의해 제작된 만주지도의 현황과 연구에 대한 소고

김두일 원광대학교 2007 圓光軍事論壇 Vol.- No.3

The purpose of this study is to survey the present situation of collection of and research on the Manchurian maps made by Imperial Japan. 'Maps on Foreign Countries' were made on vast areas from Kamchaka to the north to Australia to the south and from India to the west to Alaska to the east. The maps are now kept mainly in colleges and research institutes in addition to the National Diet Library in Japan. Such countries as USA, England, Korea and China also have maps. For example, Seoul National University in South Korea has almost complete set of 1: 500,000-scale map and several small scale maps on Manchuria. Various materials on the process of map-making exist in Japan. Several volumes of the chronicles on the Land Survey Bureau of Imperial Japan are available in addition to a few materials on the process of surveying and map-making on Manchuria. Systematic research on 'Maps on Foreign Countries' in Japan is still in the early stage, mainly focusing on the inventory of maps within Japan and abroad. Such topics as cartographic characteristics of maps, map contents and their military use, international cooperation for the study of these maps and others should be studied for the understanding of Manchurian maps.

Cisplatin에 의한 마우스의 급성 신부전 완화에 대한 Endothelin A 수용체의 차단 효과

김일두,안도환 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.1

Background : Acute renal failure (ARF) can be pathophysiologically classified to hemodynamically-mediated or nephrotoxic type, and in either case, endothelin (ET)-1 expression is enhanced in renal tissues. Hemodynamically-mediated ARF is known to be mitigated by ETA receptor (ETAR) blocker but not ETB receptor (ETBR) blocker. There is poorly understood on mitigation of renal injuries by ETAR or ETBR blockade in nephrotoxic ARF. Therefore, This study examined whether nephrotoxic ARF induced by cisplatin is functionally and/or histologically ameliorated by ETAR blocker BQ123. Materials and Methods : Male C57BL/6 mice are intraperitoneally injected with vehicle (saline), cisplatin or cisplatin+BQ123. Cisplatin was single administrated with a dose of 16 mg/kg/day and BQ123 with a dose of 24 mg/kg/day once a day for 4 consecutive days including a baseline day. Urine flow, excretion of electrolytes and protein, and creatinine clearance were measured. Histological changes were observed through periodic acid-Schiff stain. Real time RT-PCR was performed to evaluate expression of ET-1 and ETAR mRNA. Distribution of ET-1 peptide within renal cortex was examined by immunohistochemistry. Results : Body weight, urine flow, excretion of protein, Na, K, and creatinine clearance tended to decrease by cisplatin administration compared to vehicle treatment, but these parameters did not return to the normal level by combined administration of cisplatin and BQ123. Necroses of several proximal tubules were observed in the cisplatin group, indicating that mild nephrotoxicity occured. In the cisplatin+BQ123 group, this change seemed to increase, rather than decrease. Expression of ET-1 and ETAR mRNA was 6 and 2-fold higher in the cisplatin group than the saline group, respectively. BQ123 treatment reversed ETAR mRNA expression to the basal level but not ET-1 mRNA. Immnuoreactive ET-1 was mainly distributed within proximal tubule cells in all three groups. Immunoreactivity of ET-1 was enhanced in both the cisplatin group and the cisplatin+BQ123 group compared to the saline group, but was similar between the cisplatin group and the cisplatin+BQ123 group. Conclusion : Administration of a ETAR blocker BQ123 in cisplatin-induced nephrotoxic ARF is not likely to be effective in amelioration of renal injuries.